期刊
FOOD CHEMISTRY
卷 132, 期 4, 页码 1872-1882出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2011.12.020
关键词
Peptide; ACE inhibitory; Antioxidant; ROS; Antioxidative enzymes; Pacific cod skin
资金
- Marine Bioprocess Research Centre
- Ministry of Land, Transport and Maritime, Republic of Korea
Seafood processing by-product, Pacific cod (Gadus macrocephalus) skin, was utilised to purify an active peptide with ACE inhibitory and antioxidant activities. Gelatin was extracted from the skin and it was hydrolysed with gastrointestinal endopeptidases (pepsin, trypsin and alpha-chymotrypsin). Assay-guided purification of the hydrolysate resulted in an active peptide, Leu-Leu-Met-Leu-Asp-Asn-Asp-Leu-Pro-Pro (1301 Da). The peptide showed potent non-competitive ACE inhibition (IC50 = 35.7 mu M) and effectively protects cellular macromolecules from reactive oxygen species (ROS) mediated damage. The peptide significantly reduced the oxidation levels of membrane lipids, proteins and DNA in RAW264.7 cells by effectively scavenging the intracellular ROS. Moreover, it was found that the peptide treatment upregulated the m-RNA expression of cellular antioxidative enzymes (superoxide dismutase, glutathione and catalase) and thereby enhanced the intracellular antioxidant mechanisms. These findings suggest that Pacific cod skin could be effectively bioconverted to produce a bioactive peptide, which could be used as a functional food ingredient to control ACE activity and oxidative stress. (C) 2011 Elsevier Ltd. All rights reserved.
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