期刊
FOOD CHEMISTRY
卷 132, 期 1, 页码 537-543出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2011.10.054
关键词
Biogenic amines; Fish; Solid phase extraction; Liquid chromatography-tandem mass spectrometry
资金
- Universitat de Valencia [UV-ESTPC-09-5713]
Biogenic amines on fish tissue are formed as a result of bacterial contamination and spoilage during storage. A new method based on liquid chromatography (LC) and tandem mass spectrometry (MS/MS) using a triple quadrupole (QqQ) analyser was developed for the analysis of eight biogenic amines (cadaverine, histamine, phenylethylamine, putrescine, spermine, spermidine, tyramine and tryptamine) in fish tissues. Sample preparation was performed by extraction with trichloroacetic acid 5% and solid phase extraction clean up with STRATA X cartridge. The MS/MS method was validated and compared with a method based on the analysis of dansyl derivatives by LC and fluorescence detector (FD). MS/MS achieved higher sensitivity (from 0.02 mg kg(-1) for spermidine and phenylethylamine to 0.2 mg kg(-1) for spermine) when compared to FD (from 1 mg kg(-1) for putrescine and tyramine to 4 mg kg(-1) for histamine); MS/MS method showed higher precision too, with intraday relative standard deviations (RSDs) from 1% to 4% with respect to those obtained with FD method (from 3% to 17%). Recovery study was conducted at two different fortification levels and the average ranged from 71% to 93% for all of the studied compounds with RSDs lower than 18%. Matrix-matched standards were used to counteract matrix effect observed in MS/MS determination. The applicability of the method was demonstrated by the analysis of biogenic amines in fish obtained from commercials of Valencia. (C) 2011 Elsevier Ltd. All rights reserved.
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