期刊
FOOD CHEMISTRY
卷 109, 期 3, 页码 647-653出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2007.12.072
关键词
acetylgestagen; multi-residues; heterologous ELISA; fat
A new heterologous ELISA method for detecting acetylgestagen multi-residues in animal fat was developed. An antibody generated has high cross-reactivity with relative progestogens (up to 50%) and no cross-reactivity with other steroids (<0.1%) in homologous ELISA. Three heterologous immunoassay were developed and one of them improved not only sensitivity but also the class-selectivity compared with the homologous assay against these progestogens. The IC50 for four acetylgestagens, chlormadinone acetate (CMA), 17 alpha-hydroxyprogesterone acetate (HPA), megestrol acetate (MEGA) and medroxyprogesterone acetate (MPA) were, 4.5, 2.5, 2.9 and 1.8 mu g/L, respectively. The cross-reactivity for CMA, HPA and MEGA were 40%, 72% and 62% when they were compared with MPA. Progestogens recoveries from spiked swine fat averaged between 61% and 78%. Results obtained from LC/MS/MS method showed the heterologous ELISA method developed was reliable and suitable for rapid screening the four progestogens residues in fat tissues. (C) 2008 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据