Article
Biochemistry & Molecular Biology
Mohamed A. A. Abdella, Samia A. Ahmed, Mohamed E. Hassan
Summary: Protease from Bacillus thuringiensis strain-MA8 was immobilized onto Alginate/dextrose beads with an immobilization yield of 77.6%. The immobilized protease showed higher thermal stability, lower activation energy, and a longer half-life compared to the free enzyme. The Alg/dex/protease also exhibited increased Km and Vmax values, indicating enhanced catalytic efficiency. Furthermore, it displayed good reusability after multiple hydrolysis cycles.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Shalu Aggarwal, Saiqa Ikram
Summary: In this study, trypsin was effectively immobilized on the surface of ZnO/Chitosan nanocomposite through covalent attachment. The size, structure, surface morphology, and elemental composition of the nanoparticles and nanocomposite were studied. Optimal immobilization conditions and catalytic activity were investigated. The results showed improved efficiency and stability of the immobilized trypsin, suggesting its potential application in biotechnological processes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biotechnology & Applied Microbiology
Janaina de Souza Lima, Ana Paula Serafini Immich Boemo, Pedro Henrique Hermes de Araujo, Debora de Oliveira
Summary: The research found that both adsorption and covalent bonding were effective methods for immobilizing endoglucanase on kaolin, with the enzyme's catalytic activity maintained after immobilization. Adsorption was shown to be simpler, cheaper, and faster than covalent bonding, making it a promising technique for future studies.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Environmental Sciences
Hem Kanta Sharma, Chunbao (Charles) Xu, Wensheng Qin
Summary: Optimizing fermentation parameters for higher yield of bacterial enzymes is crucial for industries. Serratia marcescens HK2 shows potential in producing xylanase and glucose isomerase, utilizing low cost agricultural residues, and could be a promising candidate for food and biofuels industries.
WASTE AND BIOMASS VALORIZATION
(2021)
Article
Nanoscience & Nanotechnology
Chao Zhong, Guorong Li, Wenchang Tian, Dan Ouyang, Yin Ji, Zongwei Cai, Zian Lin
Summary: Enzyme immobilization is crucial for its application in various fields. Covalent organic frameworks (COFs) are ideal for enzyme immobilization due to their controlled porous structures. However, constructing COFs-based enzyme immobilization systems with high activity using existing methods is still challenging.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Nanoscience & Nanotechnology
Yuting Lu, Yibo Song, Lingling Peng, Xiaoping Rao, Kok Bing Tan, Shu-feng Zhou, Guowu Zhan
Summary: Metal-organic frameworks (MOFs) have shown potential as host materials for enzyme immobilization and protection. In this study, ZIF-8nanocubes were self-assembled onto yeast as a biological template to obtain hybrid Y@ZIF-8. The size, morphology, and loading efficiency of the assembled ZIF-8 nanoparticles on yeast templates could be controlled by adjusting synthetic parameters. The use of a cross-linking agent greatly enhanced the relative enzyme activity of Y@ZIF-8@t-CAT and improved its cycling stability.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biotechnology & Applied Microbiology
Mohamed E. E. Hassan, Ghada E. A. Awad, Mohamed S. S. MohyEldin, Bakry M. M. Haroun, Ahmed I. I. El-Diwany, Magdy M. M. Elnashar
Summary: Carrageenan is a common carbohydrate used in the entrapment industry for immobilizing cells and enzymes. By grafting carrageenan, it can produce fructose by covalently immobilizing glucose isomerase. Fructose is widely used in the beverage, food production, and pharmaceutical industries as a sweetener.
BIOTECHNOLOGY LETTERS
(2023)
Article
Biotechnology & Applied Microbiology
Hengmin Miao, Maosheng Li, Xiaochun Sun, Jikun Xia, Yanqing Li, Jiao Li, Fang Wang, Jiakun Xu
Summary: A series of stable mesoporous silica sieves with different pore sizes were synthesized, and the effects of pore size and adsorption methods on protein immobilization efficiency were studied. The SBA-15 with a pore size of 7.2 nm showed the best loading capacity, while the SBA-15 with a pore size of 9.8 nm showed the highest retained catalytic ability.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Environmental Sciences
Akash Balakrishnan, Mahendra Chinthala
Summary: The research on graphitic carbon nitride (g-C3N4) for energy and environmental remediation has attracted significant attention due to its excellent electronic structure and properties. However, challenges such as high recombination rate and mass loss during recovery operations have been identified. Various strategies and preparation methods for overcoming these difficulties and improving the photocatalytic applications of g-C3N4 based materials have been discussed in this review.
Article
Chemistry, Analytical
Monica Jimenez-Carretero, Ylenia Jabalera, Alberto Sola-Leyva, Maria P. Carrasco-Jimenez, Concepcion Jimenez-Lopez
Summary: The immobilization of enzymes on magnetic nanoparticles has attracted interest in detecting contaminants in water samples due to their magnetic control, concentration, and reuse capabilities. In this study, nanoassemblies composed of inorganic or biomimetic magnetic nanoparticles were developed for the detection of trace amounts of organophosphate pesticides and antibiotics in water. Enzyme immobilization was achieved through electrostatic interaction or covalent bonds, with covalent bonding yielding better results. The optimized nanoassemblies demonstrated the ability to detect pollutants at low concentrations and provided enhanced stability and reuse of the enzymes.
Article
Chemistry, Applied
Ning Chen, Baogen Chang, Nian Shi, Fuping Lu, Fufeng Liu
Summary: A novel cross-linked enzyme aggregates (CLEAs) catalyst was prepared using precipitation and cross-linking techniques for isomaltulose production. The immobilized enzyme showed excellent stability, reusability, and conversion efficiency, making it highly suitable for industrial applications.
Article
Polymer Science
Yaaser Q. Almulaiky, J. Alkabli, Reda M. El-Shishtawy
Summary: Modified polymer design has been used successfully for enzyme immobilization, offering improved stability and expanded operational conditions. In this study, amine-terminated polymers were synthesized and utilized for the capture and conversion of silver ions into silver nanoparticles for enzyme immobilization. Extensive characterization techniques were employed to investigate the structural composition and properties of the support materials. The immobilized enzymes exhibited better storage stability and broader pH and temperature adaptability compared to the free enzyme.
Article
Polymer Science
Evren Sel, Ahmet Ulu, Burhan Ates, Suleyman Koytepe
Summary: In this study, P(MMA-co-PEG500MA) copolymers were successfully utilized for CAT enzyme immobilization, with P3 sample selected as the model support achieving a high immobilization yield of 76%. The immobilized enzyme exhibited improved tolerance and stability, showcasing good reuse potential.
Article
Chemistry, Physical
Mozhgan Razzaghi, Ahmad Homaei, Roohullah Hemmati, Dariush Saberi, Soudabeh Kavousipour
Summary: Cellulose nanocrystals/gold nanoparticle hybrids were synthesized using hydrothermal treatment without toxic chemicals. The immobilized enzyme showed increased optimal temperature and retained higher activity and efficiency under extreme conditions.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Review
Chemistry, Inorganic & Nuclear
Nisha Gupta, Esmil Beliya, Jai Shankar Paul, S. K. Jadhav
Summary: This review focuses on recent research and development in the field of nanoarmoured alpha-amylase. Immobilization of alpha-amylase on nanomaterials offers the potential for improved reusability and stability. Nanomaterials, with their high surface to volume ratio and easy fabrication, provide excellent support for alpha-amylase immobilization, leading to the production of robust nanobiocatalysts.
COORDINATION CHEMISTRY REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Dilek Alagoz, S. Seyhan Tukel, Deniz Yildirim
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2015)
Article
Biochemistry & Molecular Biology
Dilek Alagoz, Deniz Yildirim, Hatice Korkmaz Guvenmez, Damla Sihay, S. Seyhan Tukel
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2016)
Article
Chemistry, Organic
Baris Binay, Dilek Alagoz, Deniz Yildirim, Ayhan Celik, S. Seyhan Tukel
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
(2016)
Article
Biochemistry & Molecular Biology
Dilek Alagoz, S. Seyhan Tukel, Deniz Yildirim
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2016)
Article
Biochemistry & Molecular Biology
Dilek Alagoz, Ayhan Celik, Deniz Yildirim, S. Seyhan Tukel, Baris Binay
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
(2016)
Article
Horticulture
Okan Ozkaya, Deniz Yildirim, Omur Dundar, S. Seyhan Tukel
SCIENTIA HORTICULTURAE
(2016)
Article
Biochemistry & Molecular Biology
Deniz Yildirim, S. Seyhan Tukel
BIOCATALYSIS AND BIOTRANSFORMATION
(2014)
Article
Biotechnology & Applied Microbiology
Deniz Yildirim, S. Seyhan Tukel, Dilek Alagoz
BIOTECHNOLOGY PROGRESS
(2014)
Article
Biochemistry & Molecular Biology
Deniz Yildirim, S. Seyhan Tukel, Ozlem Alptekin, Dilek Alagoz
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
(2013)
Article
Biochemistry & Molecular Biology
S. Seyhan Tukel, Fitnet Hurrem, Deniz Yildirim, Ozlem Alptekin
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
(2013)
Article
Biochemistry & Molecular Biology
Deniz Yildirim, S. Seyhan Tukel, Ozlem Alptekin, Dilek Alagoz
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
(2014)
Article
Biochemistry & Molecular Biology
Dilek Alagoz, S. Seyhan Tukel, Deniz Yildirim
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
(2014)
Article
Biochemistry & Molecular Biology
Deniz Yildirim, S. Seyhan Tukel
PROCESS BIOCHEMISTRY
(2013)
Article
Biotechnology & Applied Microbiology
Seyhan Tukel, Pinar Basim Sahin, Deniz Yildirim
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2013)
Article
Sermin Gul, Ramazan Bilgin, Seyhan Tukel
ASIAN JOURNAL OF CHEMISTRY
(2011)
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.