期刊
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2021.827552
关键词
myoglobin; SBA-15; immobilization; catalytic activity; reusability
资金
- National Natural Science Foundation of China [32171263]
- Special Project of Major Scientific and Technological Innovation in Shandong Province [2018SDKJ0303-1-2223]
A series of stable mesoporous silica sieves with different pore sizes were synthesized, and the effects of pore size and adsorption methods on protein immobilization efficiency were studied. The SBA-15 with a pore size of 7.2 nm showed the best loading capacity, while the SBA-15 with a pore size of 9.8 nm showed the highest retained catalytic ability.
A series of stable mesoporous silica sieves (SBA-15) with different pore sizes (9.8, 7.2, and 5.5 nm) were synthesized using a hydrothermal method. The resulting mesoporous material was then utilized for protein immobilization using myoglobin (Mb) as the target protein. The effects of pore size and adsorption methods on the immobilization efficiency of Mb in a mesoporous material were studied. The SBA-15 with a pore size of 7.2 nm showed the best loading capacity, reaching 413.8 mg/g. The SBA-15 with a pore size of 9.8 nm showed the highest retained catalytic ability (92.36%). The immobilized enzyme was more stable than the free enzyme. After seven consecutive assay cycles, Mb adsorbed by SBA-15 (Mb/SBA-15) and Mb adsorbed by SBA-15 and crosslinked with glutaraldehyde (Mb/G/SBA-15) retained 36.41% and 62.37% of their initial activity, respectively.
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