Extraction of phenolic compounds from Vitex agnus-castus L.

The primary objective of this study was to valorized Vitex agnus-castus residues in terms of phenolic compounds. The effects of extraction time (30-360 min), solid to liquid ratio (0.1-0.3 g(DryBiomass)/ml(Solvent)), type of solvent and different tissue types (leave, roots and seeds) on total polyphenols, o-diphenols, total flavonoids and anthocyanins were evaluated. The highest total polyphenol (31.5 mg(CaffeicAcidEquivalent)/g(DryBiomass)) and o-diphenol (12.4 mg(CaffeicAcidEquivalent)/g(DryBiomass)) contents were obtained from methanolic extract of leaves after 180 min using a solid/liquid ratio of 0.1 g(DryBiomass)/ml(Solvent), while total flavonoids, reached a maximum value of 19.4 mg(CatechinEquivalent)/g(DryBiomass) after 360 min under the same conditions. Roots of V. agnus-castus were found to be a good source of anthocyanins with the highest yield of 0.62 mg(MalvidinEquivalent)/g(DryBiomass) using ethanol as a solvent (180 min and 0.2 g(DryBiomass)/ml(Solvent)). The maximum antiradical power (178.5 mu l(extract)/mu g(DPPH)) was exhibited by the methanolic leave extract obtained after 360 min at solid/liquid ratio of 0.3 g(DryBiomass)/ml(Solvent). (C) 2012 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.