期刊
FOOD AND AGRICULTURAL IMMUNOLOGY
卷 26, 期 3, 页码 317-324出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2014.914469
关键词
EDC; nadifloxacin; chicken muscle; indirect competitive enzyme-linked immunosorbent assay (ELISA)
资金
- Key Programmes from MOST [2012BAD29B04]
- MOF
- MOE [201310135, JUSRP51308A]
A convenient and specific competitive enzyme-linked immunosorbent assay (ELISA) for nadifloxacin (NDF) was developed. The modified 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) method was employed to synthesise the artificial antigen of NDF. The indirect competitive ELISA developed to detect NDF has a concentration at 50% inhibition (IC50) of 0.72 ng/mL and a limit of detection of 0.04 ng/mL. This ELISA kit could be used as a screening method to detect and control the illegal content of NDF in food products. In this study, the kit was applied to NDF detection in chicken muscle and produced a satisfactory result with recovery rates from samples spiked with NDF of 92% (10 ng/mL) and 101% (50 ng/mL).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据