期刊
FOOD AND AGRICULTURAL IMMUNOLOGY
卷 26, 期 2, 页码 204-217出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2014.884056
关键词
residue; malachite green; indirect competitive; chemiluminescent enzyme immunoassay
资金
- National Natural Science Foundation of China [31201361]
- Science and Technology Project of Guangzhou City [11BppZXcc2100021]
- Science and Technology Project of Guangdong Province [2010A032000001-4, 2012A020100002]
- National Spark Program [2012GA780001]
- Program of Pearl River Young Talents of Science and Technology in Guangzhou, China [2013J2200080]
An indirect competitive chemiluminescent enzyme immunoassay method was developed for screening malachite green (MG). Assay conditions, including the concentration of coating, antibody dilution, incubation time, dilution buffer, ionic strength and pH, were optimised. Under the optimised conditions, coating antigen concentration was 125 ng/mL; and dilution fold of antibody was 40,000. The 50% inhibition values of 0.22 ng/mL for MG were achieved with a limit of detection of 0.01 ng/mL, the linear range was from 0.03 ng/mL to 3.27 ng/mL. The assay showed a little cross-reactivity of 3.4%, 2.7% and 1.0% with methylene blue, brilliant green and crystal violet, respectively, and negligible cross-reactivity with other analogues of MG. The developed method has been used to quantify MG in seafood samples. The recovery of MG ranged from 82.43% to 108.0% at four concentrations (0.1, 1, 3 and 5 ng/mL), and the coefficients of variation were less than 13%. A comparison results between the high-performance liquid chromatography and the developed assay showed good relativity. The results indicated that the assay was a sensitive and stable method for screening MG.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据