Quantification of 2-Acetyl-1-pyrroline and Other Rice Aroma Volatiles Among Indian Scented Rice Cultivars by HS-SPME/GC-FID

A method for quantitative determination of 2-Acetyl-1-pyrroline (2AP) and other aroma volatiles using HS-solid phase micro extraction (SPME)/GC-FID in scented rice has been developed. Extraction at 80 A degrees C for 30 min pre-incubation followed by 20-min adsorption from 1 g rice containing 300 mu l of odour-free water were the optimum conditions for quantification. Calibration curves of aroma volatiles were obtained by standard addition approach. The optimised conditions were employed for quantitative analysis in 33 scented and two non-scented rice samples. Highest amount of 2AP was recorded in Indrayani Brand 2 (0.552 ppm), followed by Kamod (0.418 ppm) and Basmati Brand 5 (0.411 ppm). Rice types (Basmati, Ambemohar, Kolam, Indrayani and local) significantly contributed to the variation in 2AP, hexanal, nonanal, decanal, benzyl alcohol, vanillin, guaiacol and indole. The method developed suffices the need of rapid and reliable method for quantification of 2AP and other aroma-related volatiles from aromatic rice cultivars.