4.3 Article

Colonization and probiotic effects of lactic acid bacteria in the gut of the abalone Haliotis gigantea

期刊

FISHERIES SCIENCE
卷 75, 期 5, 页码 1285-1293

出版社

SPRINGER JAPAN KK
DOI: 10.1007/s12562-009-0138-5

关键词

Abalone; Colonization; Lactic acid bacteria (LAB); Probiotics; Volatile short-chain fatty acid (VSCFA)

资金

  1. Ministry of Education, Science, and Culture of Japan [19880014]
  2. Grants-in-Aid for Scientific Research [19880014] Funding Source: KAKEN

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Strains of lactic acid bacteria (LAB) were isolated from several different sources and evaluated in vitro for potential probiotic effects in abalones. Two isolates (Lactobacillus sp. strain a3 and Enterococcus sp. strain s6) were highly resistant to bile salt and/or gastric juice and inhibited the growth of three abalone pathogens (Listonella anguillarum, Vibrio harveyi, and V. carchariae). Each of the LAB isolates was used to supplement diet of the abalone Haliotis gigantea for a period of 3 weeks. One group of animals received Lactobacillus sp. strain a3 added to commercial dry feed, one group received Enterococcus sp. strain s6 added to the feed, and a control group received only standard commercial feed. Culturable LAB counts of gut homogenates indicate the a3 colonized in the gut of abalones. Digestive enzyme activities and the concentrations of a number of volatile short-chain fatty acids (VSCFA) were elevated in the gut of abalones receiving feed supplemented with the two LAB strains. These results indicate that dietary supplementation can enable LAB colonization or persistence in the gut of abalone species and can potentially enhance probiotic effects.

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