Article
Chemistry, Applied
Ran Li, Shuhong Li, Zhiguang Chen, Yu Jin, Shulei Li, Song Li, Zhizi Bai
Summary: The study on grass carp stefin A gene reveals its heat and acid-base tolerance, inhibitory effects on enzymes, and highest expression level in the hepatopancreas. The results provide insights into the physical and biochemical properties of grass carp stefin A.
Article
Immunology
Changyong Mu, Qiwang Zhong, Yan Meng, Yong Zhou, Nan Jiang, Wenzhi Liu, Yiqun Li, Mingyang Xue, Lingbing Zeng, Vikram N. Vakharia, Yuding Fan
Summary: In this study, a recombinant baculovirus expressing VP35-VP4 proteins from GCRV was generated and used to vaccinate grass carp through oral administration. The results showed that the vaccine induced both non-specific and specific immune responses, providing protection against GCRV infection with a relative percent survival rate of 56%. This study suggests that the silkworm pupae powder could be used as an effective oral vaccine for grass carp.
Article
Fisheries
Hongquan Wang, Hong Yang, Rui Li, Yaoguo Li, Tiaoyi Xiao
Summary: By comparing the expression variation of 13 immune factors in different tissues, it was found that the high gene expression in the coagulation complement and cytokine interaction pathways may be the main reason for the strong resistance of barbel chub to GCRV. However, high gene expression was not a factor affecting GCRV resistance between grass carp populations.
AQUACULTURE INTERNATIONAL
(2021)
Article
Fisheries
Chaobin Qin, Wenli Zhao, Xiao Yan, Guokun Yang, Liping Yang, Ronghua Lu, Daming Pi, Guoxing Nie
Summary: The study suggests that adiponectin plays critical roles in regulating glucose metabolism in fish. It promotes glycolysis, gluconeogenesis, and glycogen synthesis, increases glycogen content in the hepatopancreas, and reduces basal blood glucose level. Adiponectin A and adiponectin B have opposite regulatory effects on glucose uptake.
AQUACULTURE NUTRITION
(2022)
Article
Fisheries
Pengfei Chu, Yuchao Zhu, Luqian Xu, Xiangyu Yao, Yan Liang, Xiaojun Zhang
Summary: This study identified and analyzed the functional role of ATG4C, a protein involved in autophagy, in grass carp. ATG4C was found to be widely distributed in grass carp tissues, with high expression in the intestine. It showed a strong response in immune tissues after infection with grass carp reovirus (GCRV), indicating its involvement in the immune response. Additionally, the study confirmed the induction of autophagy and apoptosis by GCRV invasion, and revealed that ATG4C positively regulated autophagy while restricting apoptosis, leading to inhibition of GCRV replication.
Article
Fisheries
Wei Wei, Junya Wang, Qingyu Min, Zhao Jia, Kangyong Chen, Hao Feng, Jun Zou
Summary: The study shows that CCL19a2 and CCL19b can promote migration of immune cells and are involved in recruitment of antigen presenting cells in fish. These chemokines exhibit activity and significance both in vitro and in vivo.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Immunology
Qingqing Tian, Xingchen Huo, Qian Liu, Chunrong Yang, Yongan Zhang, Jianguo Su
Summary: This study developed a prophylactic grass carp reovirus (GCRV) vaccine using virus-like particle (VLP) assembly technology, and the highly immunogenic candidates in the vaccine were VP4, VP56, and VP35. Expression of VP4, VP56, and VP35 in both Escherichia coli and Pichia pastoris systems resulted in successful assembly of uniform, stable, and non-toxic VP4/VP56/VP35 VLPs. Vaccination with P. pastoris VLPs significantly improved survival rate, IgM levels, tissue viral load, and antioxidant immune-related indicators in grass carp.
Article
Fisheries
Jianhua Feng, Zhao Jia, Gaoliang Yuan, Xiaozhen Zhu, Qin Liu, Kaizheng Wu, Junya Wang, Jun Zou
Summary: This study investigates the expression and antibacterial activities of three grass carp beta-defensin peptides. The results show that these peptides are constitutively expressed in tissues, with the highest expression in the skin. They also demonstrate broad antibacterial activity against fish bacterial pathogens.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Fisheries
Baohong Xu, Hang Su, Changjun Chen, Qiaolin Liu, Tiaoyi Xiao, Yi Liu, Zhao Lv, Hongquan Wang, Zheyu Wen
Summary: The interaction of C5a and C5aR in grass carp infected with grass carp reovirus promotes the activation of p38 and ERK proteins, leading to increased expression of IL-6 and TNF-alpha and induction of an inflammatory response. These findings provide a theoretical basis for regulating the interaction of C5a and C5aR to alleviate grass carp hemorrhagic diseases.
AQUACULTURE REPORTS
(2023)
Article
Virology
Rui Jiang, Jie Zhang, Zhiwei Liao, Wentao Zhu, Hang Su, Yongan Zhang, Jianguo Su
Summary: This study investigated the latent infection of GCRV-II in asymptomatic grass carp with GCRV infection or exposure history. It was found that GCRV-II was detectable only in the brain of grass carp during latent infection, unlike the multi-tissue distribution observed in natural infection. Additionally, GCRV-II distribution in grass carp was notably affected by ambient temperature. This study provides insights into the mechanisms of GCRV-II latent infection and reactivation, contributing to the prevention and control of GCRV pandemics.
Article
Virology
Ling Yang, Jianguo Su
Summary: In this study, it was found that GCRV can only infect leukocytes in vitro, while erythrocytes and thrombocytes are not susceptible. Additionally, the expression levels of immune response-related genes were significantly up-regulated in infected leukocytes.
Article
Fisheries
Guokun Yang, Xiaomin Liang, Shuangyang Xu, Huimin Cai, Limei Ma, Xulu Chang, Yanmin Zhang, Liping Yang, Xiaolin Meng
Summary: This study focused on the involvement of Igf3 in fish reproduction, particularly in grass carp, by isolating the gene and assessing its functions through various experiments. Igf3 was found to regulate gene expression in multiple tissues, affecting growth-related genes and glucose metabolism. This research is the first to reveal the mechanism by which Igf3 regulates the expression of growth-related genes in teleosts.
Article
Virology
Wentao Zhu, Meihua Qiao, Meidi Hu, Xingchen Huo, Yongan Zhang, Jianguo Su
Summary: This study found that Type II grass carp reovirus (GCRV-II) rapidly invades grass carp through the nostril, gill, and skin on the head, rather than merely by adhesion. Further investigations revealed that GCRV-II invades grass carp via the nostril-olfactory system-brain axis, and viral replication occurs.
Article
Fisheries
Xiaoqing Xiong, Hong Yang, Chunhua Ding, Beibei Qin, Yadong Deng, Liming Xiong, Xiaoyan Liu, Yaoguo Li, Tiaoyi Xiao, Zhao Lv
Summary: In this study, a newly discovered integrin αvβ3 in grass carp was found to interact with viral proteins and mediate antiviral immune response. Furthermore, the expression of this integrin was significantly upregulated in various tissues of grass carp after infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Yong Jin, Li Li, Jun Hua Li, Pin Nie
Summary: In this study, the PGRP2 gene and its variants were cloned and characterized in grass carp, showing their expression and activation in different tissues. This provides important insights into the PGRP members in grass carp.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Virology
Yang Gao, Xingchen Huo, Zhensheng Wang, Gailing Yuan, Xiaoling Liu, Taoshan Ai, Jianguo Su
Summary: This study successfully prepared an oral immunization vaccine using Bacillus subtilis VP56-2 subunit, which effectively increased the survival rate of grass carp and enhanced important immune markers, alleviated tissue lesions, and reduced viral load after viral challenge.
Review
Fisheries
Wentao Zhu, Jianguo Su
Summary: Phagocytosis is a fundamental immune function against foreign microorganisms and abnormal cells, with blood being a crucial site for this process. Professional phagocytes such as monocytes/macrophages and neutrophils play a primary role in phagocytosis and pathogen treatment, while non-professional phagocytes including thrombocytes, B cells, and erythrocytes perform auxiliary roles in phagocytosis and exercise unique immune functions in the teleost immune system.
REVIEWS IN AQUACULTURE
(2022)
Article
Immunology
Chi Zhang, An-Qi Liu, Chu Zhang, Lan-Hao Liu, Jianguo Su, Yong-An Zhang, Jiagang Tu
Summary: This study reveals the virus-host combat mechanism between Cyprinid herpesvirus 3 (CyHV-3) and its host. It shows that CyHV-3 evades host antiviral immunity through its ORF89 protein, while the upregulated host miR-722 targets ORF89 to impede CyHV-3 replication.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Fisheries
Jiao Chang, Wentao Zhu, Xingchen Huo, Meihua Qiao, Chunrong Yang, Yongan Zhang, Jianguo Su
Summary: In this study, a recombinant Lactobacillus casei expressing multiple fragments of the key outer capsid protein of grass carp reovirus (GCRV) was constructed, and an oral microcapsule live vaccine (SCL-56310-500) was prepared. The results showed that this vaccine effectively enhanced the immune response and suppressed GCRV replication in fish, leading to a significantly higher survival rate.
Article
Fisheries
Yanqi Zhang, Jianguo Su
Summary: This review discusses the unique biological effects of IL-2 family cytokines in regulating differentiation, survival, and activation of multiple lymphocyte lineages, as well as their expression profiles, receptors, signaling transductions, and functions in teleosts. The IL-2 family cytokines in teleosts show relatively conserved functions in lymphocyte proliferation, differentiation, development, and inflammation, similar to their counterparts in mammals.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Immunology
Wentao Zhu, Yanqi Zhang, Zhiwei Liao, Xingchen Huo, Chunrong Yang, Yongan Zhang, Jianguo Su
Summary: This study reveals the strong antibacterial activity of type I interferon IFN1 in teleosts, which is mediated by phagocytosis and induction of immune factors and complement components. These findings shed light on the functional role of interferons in bacterial infections.
JOURNAL OF IMMUNOLOGY
(2023)
Article
Fisheries
Weixiang Zhang, Zhenjun Zhao, Jiancheng Zhou, Weicheng Wang, Jianguo Su, Gailing Yuan
Summary: This study evaluates the feasibility and biological activity of carboxymethyl chitosan (CMCS) loaded with Ctenopharyngodon idella interferon-gamma 2 (CiIFN-gamma 2), and examines the resistance to Aeromonas hydrophila infection in grass carp. The results show that CMCS-CiIFN-gamma 2 nanoparticles can enhance the expression of pro-inflammatory cytokines and the effective uptake of the protein, leading to improved immune protection against bacterial diseases in aquaculture.
Article
Fisheries
Qian Liu, Xingchen Huo, Qingqing Tian, Pengxu Wang, Fengxia Zhao, Chunrong Yang, Jianguo Su
Summary: The oral P-MCP-FlaC vaccine effectively controls LMBV infection in largemouth bass, improving the survival rate and up-regulating protective enzyme activity and immune gene expression.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Aotian Ouyang, Mengwei Zhang, Gailing Yuan, Xiaoling Liu, Jianguo Su
Summary: This study reveals that chitooligosaccharide (COS) has the ability to reverse cortisol-induced immunosuppression in fish and enhance the immune activity of macrophages. Oral administration of COS directly absorbed through the intestine significantly improves the innate immunity of blunt snout bream, leading to increased survival and reduced tissue damage. Thus, COS offers potential strategies for immunosuppression prevention and control in fish.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Virology
Wentao Zhu, Meihua Qiao, Meidi Hu, Xingchen Huo, Yongan Zhang, Jianguo Su
Summary: This study found that Type II grass carp reovirus (GCRV-II) rapidly invades grass carp through the nostril, gill, and skin on the head, rather than merely by adhesion. Further investigations revealed that GCRV-II invades grass carp via the nostril-olfactory system-brain axis, and viral replication occurs.
Article
Immunology
Qingqing Tian, Xingchen Huo, Qian Liu, Chunrong Yang, Yongan Zhang, Jianguo Su
Summary: This study developed a prophylactic grass carp reovirus (GCRV) vaccine using virus-like particle (VLP) assembly technology, and the highly immunogenic candidates in the vaccine were VP4, VP56, and VP35. Expression of VP4, VP56, and VP35 in both Escherichia coli and Pichia pastoris systems resulted in successful assembly of uniform, stable, and non-toxic VP4/VP56/VP35 VLPs. Vaccination with P. pastoris VLPs significantly improved survival rate, IgM levels, tissue viral load, and antioxidant immune-related indicators in grass carp.
Article
Multidisciplinary Sciences
Rui Jiang, Wentao Zhu, Zhiwei Liao, Chunrong Yang, Jianguo Su
Summary: TLR7 plays a crucial role in sensing viral ssRNA and dsRNA, but there are differences in the mechanism of dsRNA sensing between fish and tetrapods.
Article
Virology
Rui Jiang, Jie Zhang, Zhiwei Liao, Wentao Zhu, Hang Su, Yongan Zhang, Jianguo Su
Summary: This study investigated the latent infection of GCRV-II in asymptomatic grass carp with GCRV infection or exposure history. It was found that GCRV-II was detectable only in the brain of grass carp during latent infection, unlike the multi-tissue distribution observed in natural infection. Additionally, GCRV-II distribution in grass carp was notably affected by ambient temperature. This study provides insights into the mechanisms of GCRV-II latent infection and reactivation, contributing to the prevention and control of GCRV pandemics.
Article
Fisheries
Pengxu Wang, Xingchen Huo, Fengxia Zhao, Qian Liu, Qingqing Tian, Chunrong Yang, Jianguo Su
Summary: This study aimed to investigate the potential effectiveness of VD3 in treating Largemouth bass ranavirus (LMBV). The results showed that feeding Largemouth bass with 15000 IU/Kg and 20000 IU/Kg of VD3 significantly suppressed and cleared LMBV pathogens. In addition, VD3 treatment increased the expression levels of key immune-related genes and enhanced antioxidant and immune activities in the serum. Tissue pathology sections also revealed a dose-dependent relationship between VD3 supplementation and tissue damage.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Maolin Lv, Jingjing Zhang, Weicheng Wang, Rui Jiang, Jianguo Su
Summary: This study corrected the number of grass carp TLR20 members, identified a new TLR20 member, and analyzed its evolutionary and structural characteristics. The results showed that TLR20 plays an important role in antiviral and antibacterial defense.
FISH AND SHELLFISH IMMUNOLOGY REPORTS
(2023)
