Production of prone-to-aggregate proteins

A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli

(2013) Simone Balzer et al.   Microbial Cell Factories

Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels

(2013) Susan Schlegel et al.   Microbial Cell Factories

Unusual biophysics of intrinsically disordered proteins

(2012) Vladimir N. Uversky   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm

(2012) Julie Lobstein et al.   Microbial Cell Factories

Specific Recognition of p53 Tetramers by Peptides Derived from p53 Interacting Proteins

(2012) Ronen Gabizon et al.   PLoS One

Stabilizing Additives Added during Cell Lysis Aid in the Solubilization of Recombinant Proteins

(2012) David J. Leibly et al.   PLoS One

The Ability to Enhance the Solubility of Its Fusion Partners Is an Intrinsic Property of Maltose-Binding Protein but Their Folding Is Either Spontaneous or Chaperone-Mediated

(2012) Sreejith Raran-Kurussi et al.   PLoS One

Single pH buffer refolding screen for protein from inclusion bodies

(2012) Bruno Coutard et al.   PROTEIN EXPRESSION AND PURIFICATION

Tobacco etch virus protease retains its activity in various buffers and in the presence of diverse additives

(2012) Changsheng Sun et al.   PROTEIN EXPRESSION AND PURIFICATION

Interactions of formulation excipients with proteins in solution and in the dried state

(2011) Satoshi Ohtake et al.   ADVANCED DRUG DELIVERY REVIEWS

Molecular origins of surfactant-mediated stabilization of protein drugs

(2011) Hyo Jin Lee et al.   ADVANCED DRUG DELIVERY REVIEWS

The N-domain of Escherichia coli phosphoglycerate kinase is a novel fusion partner to express aggregation-prone heterologous proteins

(2011) Jong-Am Song et al.   BIOTECHNOLOGY AND BIOENGINEERING

Chemical Synthesis and Expression of the HIV-1 Rev Protein

(2011) Peter Siman et al.   CHEMBIOCHEM

The variable detergent sensitivity of proteases that are utilized for recombinant protein affinity tag removal

(2011) James M. Vergis et al.   PROTEIN EXPRESSION AND PURIFICATION

An overview of enzymatic reagents for the removal of affinity tags

(2011) David S. Waugh   PROTEIN EXPRESSION AND PURIFICATION

Selection of soluble protein expression constructs: the experimental determination of protein domain boundaries

(2010) Michael R. Dyson   BIOCHEMICAL SOCIETY TRANSACTIONS

Production of a Soluble Disulfide Bond-Linked TCR in the Cytoplasm of Escherichia coli trxB gor Mutants

(2010) Nathaniel Liddy et al.   MOLECULAR BIOTECHNOLOGY

Screening optimized protein purification protocols by coupling small-scale expression and mini-size exclusion chromatography

(2010) Elisa Sala et al.   PROTEIN EXPRESSION AND PURIFICATION

Effect of Additives on Protein Aggregation

(2009) Hiroyuki Hamada et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

Mechanisms of Protein Aggregation

(2009) John Philo et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

Potential inaccurate quantitation and sizing of protein aggregates by size exclusion chromatography: Essential need to use orthogonal methods to assure the quality of therapeutic protein products

(2009) John F. Carpenter et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Use of folding modulators to improve heterologous protein production in Escherichia coli

(2009) Olga Kolaj et al.   Microbial Cell Factories

The C-terminal domain of the HIV-1 Vif protein is natively unfolded in its unbound state

(2009) T. H. Reingewertz et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Thioredoxins and glutaredoxins as facilitators of protein folding

(2008) Carsten Berndt et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Protein aggregation kinetics, mechanism, and curve-fitting: A review of the literature

(2008) Aimee M. Morris et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

The Structure and Interactions of the Proline-rich Domain of ASPP2

(2008) Shahar Rotem et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Hyper-acidic protein fusion partners improve solubility and assist correct folding of recombinant proteins expressed in Escherichia coli

(2008) Zhurong Zou et al.   JOURNAL OF BIOTECHNOLOGY

Molecular basis of the interaction between the antiapoptotic Bcl-2 family proteins and the proapoptotic protein ASPP2

(2008) C. Katz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Tuning Escherichia coli for membrane protein overexpression

(2008) S. Wagner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study

(2008) Nicola A. Burgess-Brown et al.   PROTEIN EXPRESSION AND PURIFICATION

The impact of kosmotropes and chaotropes on bulk and hydration shell water dynamics in a model peptide solution

(2007) Daniela Russo   CHEMICAL PHYSICS