Article
Biotechnology & Applied Microbiology
Yanjuan Liu, Mengru Zhang, Hang Yuan, Zhurong Zou
Summary: The study enhanced the activity of Escherichia coli DnaJ by using a hyper-acidified protein fusion strategy, showing improved solubility and activity of target proteins. The hyper-acidified DnaJ variants not only aid in correct folding of proteins into soluble and active forms, but also enhance heat tolerance in E. coli or yeast.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Oliver Millington, Stephanie Montanaro, Anastasia Leventis, Ashish Sharma, Simon A. Dowland, Nipun Sawhney, Kealan J. Fallon, Weixuan Zeng, Daniel G. Congrave, Andrew J. Musser, Akshay Rao, Hugo Bronstein
Summary: This work designs, synthesizes, and characterizes a series of novel dimers based on the high-triplet-energy, SF-active chromophore, 1,6-diphenyl-1,3,5-hexatriene (DPH). Transient absorption spectroscopy and fluorescence lifetime studies show that five of the dimers display iSF activity, with varying time constants for singlet fission. Close spatial proximity and minimal through-bond communication are crucial for balancing the rate of SF against the reverse recombination process.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biotechnology & Applied Microbiology
Jinhak Kwon, Hyeongjun Cho, Seungmin Kim, Yiseul Ryu, Joong-Jae Lee
Summary: Enterokinase plays a role in regulating biological processes in intestinal mucosa and accelerating protein digestion. A production strategy using solubility enhancers like thioredoxin, phosphoglycerate kinase, or maltose-binding protein can enhance solubility and bacterial expression level of enterokinase. This demonstrates the potential of combinatorial design of fusion proteins for efficient production of recombinant proteins in bacteria.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Chemistry, Physical
Lichun Sun, Yichuan Chen, Mengtao Sun
Summary: The study proposes a method to measure ESIPT using plasmon-enhanced hyper-Raman scattering and two-photon excitation fluorescence, allowing observation of very weak emissions. Plasmon can enhance resonance absorption and fluorescence simultaneously.
JOURNAL OF PHYSICAL CHEMISTRY C
(2022)
Article
Biochemistry & Molecular Biology
Ying Wang, Siqi Li, Ze Zhou, Lifen Sun, Jing Sun, Chuanpu Shen, Ranran Gao, Jingyuan Song, Xiangdong Pu
Summary: This study elucidated the functional characteristics of CsCCD2, including its catalytic activity towards different substrates. The results showed that using specific tags and optimizing expression conditions resulted in increased soluble production of CsCCD2 protein. This study is significant for the resolution of CsCCD2 crystal structure and the synthesis of crocins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xinyu Wang, Jiaxuan Li, Xiaoshan Lin, Yi Zhang
Summary: The s-oph enzyme, derived from the cloned s-oph gene, showed efficient degradation of PVA with high specificity and stability. This enzyme has the potential for practical application in alleviating plastic pollution and protecting the environment.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Xinyu Wang, Jiaxuan Li, Xiaoshan Lin, Yi Zhang
Summary: This study successfully obtained an enzyme capable of efficiently, specifically, and stably degrading PVA by cloning and expressing the s-oph gene. This enzyme has good potential for practical application in ameliorating plastic pollution and protecting the environment.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Chemistry, Physical
Huaxin Zhang, Honglin Wang, Hongmei Yang, Dan Zhou, Qinghua Xia
Summary: Water-soluble amino-terminated SiQDs were successfully synthesized without extra reductants or catalysts, showing excellent fluorescence properties and stability. SiQDs could stably bind with human serum albumin and have potential biomedical applications.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Chemistry, Applied
Ying Li, Zhong Zhang, Alireza Abbaspourrad
Summary: The study found that soluble complexes were achieved with iota-carrageenan, xanthan gum, and high-methoxyl pectin when complexed with phycocyanin, showing good colloidal stability without phase separation at pH 3.0 before and after heating. The electrostatic complexation between phycocyanin and these three polysaccharides was reinforced by hydrophobic interactions, with the iota-carrageenan-phycocyanin complex exhibiting the best colloidal and color stabilities.
FOOD HYDROCOLLOIDS
(2021)
Article
Biochemistry & Molecular Biology
Cara L. Loomis, Simone Brixius-Anderko, Emily E. Scott
Summary: The study finds that adrenodoxin can regulate the ligand binding and catalytic activity of CYP11B1 enzyme, reducing the dissociation constant of substrate binding and the inhibitory effect of inhibitors. Comparison between different CYP11B enzymes reveals significant differences in the effects of adrenodoxin, likely due to variations in their sequences and structures. These findings underscore the importance of considering P450/redox partner interactions when evaluating new inhibitors.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Nathania Puspitasari, Cheng-Kang Lee
Summary: Hydrophobins are highly surface-active proteins that can reverse surface hydrophobicity through self-assembly at interfaces, with potential applications in surface functional modifications. By fusing cellulose-binding domain with hydrophobins, soluble expression in E. coli can be enhanced for rapid isolation and purification. This strategy also retains the interfacial activity of hydrophobins and accelerates PETase hydrolysis against PET fiber.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Lidia Ruiz-Llorente, M. Cristina Vega, Francisco J. Fernandez, Carmen Langa, Nicholas W. Morrell, Paul D. Upton, Carmelo Bernabeu
Summary: sEng is the soluble form of endoglin, expressed at high levels in various diseases. It serves as a marker of cardiovascular damage and tumor development, plays a pathogenic role in conditions like preeclampsia, and inhibits angiogenesis and tumor proliferation, migration, and invasion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Baixi Zhang, Tong Zhu, Xintian Huang
Summary: This study successfully improved the solubility of recombinant linoleic acid isomerase in Escherichia coli by exploring different combinations of promoters and fusion tags. Among the nine recombinant strains, the one containing the T7 promoter and MBP fusion tag showed the highest solubility at 86.2%. The study also investigated the optimal catalytic conditions and inhibitory substances for MBP-PAI.
Article
Food Science & Technology
Akihiro Nakamura, Hitomi Ohboshi, Madoka Sakai, Kei Nomura, Setsuko Nishiyama, Hiroko Ashida
Summary: This study investigated the extraction conditions and properties of water-soluble polysaccharides from kidney bean fibers, highlighting their protein dispersing and stabilizing properties under acidic conditions.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Multidisciplinary Sciences
Shashank Shekhar, Andrew T. Moehlman, Brenden Park, Michael Ewnetu, Charles Tracy, Iris Titos, Krzysztof Pawlowski, Vincent S. Tagliabracci, Helmut Kramer
Summary: The study reveals that Allnighter (Aln), a pseudokinase, plays a crucial role in maintaining normal sleep and neuronal structural plasticity in Drosophila photoreceptors by regulating proteostasis. Aln protein is secreted by different neurons but not expressed in photoreceptors, yet it is retrogradely endocytosed by photoreceptors. Inhibition of photoreceptor synaptic release reduces Aln levels in lamina neurons, indicating the involvement of secreted Aln in a feedback loop. Moreover, aln mutants exhibit reduced night time sleep, suggesting a molecular link between dysregulated proteostasis and sleep, which are characteristics of ageing and neurodegenerative diseases.
NATURE COMMUNICATIONS
(2023)