Engineering stereoselectivity of ThDP-dependent enzymes

(S)-Selective MenD variants from Escherichia coli provide access to new functionalized chiral α-hydroxy ketones

(2013) Robert Westphal et al.   CHEMICAL COMMUNICATIONS

CC bond formation using ThDP-dependent lyases

(2013) Michael Müller et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

TCA Cycle Involved Enzymes SucA and Kgd, as well as MenD: Efficient Biocatalysts for Asymmetric C–C Bond Formation

(2013) Maryam Beigi et al.   ORGANIC LETTERS

Improving Transketolase

(2013) Adeline Ranoux et al.   TOPICS IN CATALYSIS

Influence of Organic Solvents on Enzymatic Asymmetric Carboligations

(2012) Tina Gerhards et al.   ADVANCED SYNTHESIS & CATALYSIS

A standard numbering scheme for thiamine diphosphate-dependent decarboxylases

(2012) Constantin Vogel et al.   BMC BIOCHEMISTRY

Enhancement of the Substrate Scope of Transketolase

(2012) Adeline Ranoux et al.   CHEMBIOCHEM

A pH-Based High-Throughput Assay for Transketolase: Fingerprinting of Substrate Tolerance and Quantitative Kinetics

(2012) Dong Yi et al.   CHEMBIOCHEM

Many of the functional differences between acetohydroxyacid synthase (AHAS) isozyme I and other AHASs are a result of the rapid formation and breakdown of the covalent acetolactate-thiamin diphosphate adduct in AHAS I

(2012) Inna Belenky et al.   FEBS Journal

Crystal structure of a ring-cleaving cyclohexane-1,2-dione hydrolase, a novel member of the thiamine diphosphate enzyme family

(2012) Alma Steinbach et al.   FEBS Journal

Modular microfluidic reactor and inline filtration system for the biocatalytic synthesis of chiral metabolites

(2012) Brian O'Sullivan et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Rational substrate and enzyme engineering of transketolase for aromatics

(2012) Panwajee Payongsri et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Benzaldehyde lyase-catalyzed diastereoselective C–C bond formation by simultaneous carboligation and kinetic resolution

(2012) Christoph R. Müller et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

α-Hydroxy-β-keto acid rearrangement–decarboxylation: impact on thiamine diphosphate-dependent enzymatic transformations

(2012) Maryam Beigi et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Significant Catalytic Roles for Glu47 and Gln 110 in All Four of the C−C Bond-Making and -Breaking Steps of the Reactions of Acetohydroxyacid Synthase II

(2011) Maria Vyazmensky et al.   BIOCHEMISTRY

Using Substrate Analogues To Probe the Kinetic Mechanism and Active Site ofEscherichia coliMenD

(2011) Maohai Fang et al.   BIOCHEMISTRY

S-Selective Mixed Carboligation by Structure-Based Design of the Pyruvate Decarboxylase from Acetobacter pasteurianus

(2011) Dörte Rother neé Gocke et al.   ChemCatChem

Cation–π interactions: Complexes of guanidinium and simple aromatic systems

(2011) Fernando Blanco et al.   CHEMICAL PHYSICS LETTERS

C−C Bond-Forming Lyases in Organic Synthesis

(2011) Margarita Brovetto et al.   CHEMICAL REVIEWS

Functional Plasticity and Allosteric Regulation of α-Ketoglutarate Decarboxylase in Central Mycobacterial Metabolism

(2011) Tristan Wagner et al.   CHEMISTRY & BIOLOGY

Oxidation-hydroxymethylation-reduction: a one-pot three-step biocatalytic synthesis of optically active α-aryl vicinal diols

(2011) Saravanakumar Shanmuganathan et al.   GREEN CHEMISTRY

Cyclohexane-1,2-Dione Hydrolase from Denitrifying Azoarcus sp. Strain 22Lin, a Novel Member of the Thiamine Diphosphate Enzyme Family

(2011) A. K. Steinbach et al.   JOURNAL OF BACTERIOLOGY

Directed evolution to re-adapt a co-evolved network within an enzyme

(2011) John Strafford et al.   JOURNAL OF BIOTECHNOLOGY

Stability, activity, and selectivity of benzaldehyde lyase in supercritical fluids

(2011) Dessy Natalia et al.   JOURNAL OF SUPERCRITICAL FLUIDS

Conversion of Pyruvate Decarboxylase into an Enantioselective Carboligase with Biosynthetic Potential

(2011) Danilo Meyer et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Benzaldehyde lyase catalyzed enantioselective self and cross condensation reactions of acetaldehyde derivatives

(2011) Peruze Ayhan et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Enantioselective Intermolecular Aldehyde-Ketone Cross-Coupling through an Enzymatic Carboligation Reaction

(2010) Patrizia Lehwald et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Double Duty for a Conserved Glutamate in Pyruvate Decarboxylase: Evidence of the Participation in Stereoelectronically Controlled Decarboxylation and in Protonation of the Nascent Carbanion/Enamine Intermediate,

(2010) Danilo Meyer et al.   BIOCHEMISTRY

The Thiamine diphosphate dependent Enzyme Engineering Database: A tool for the systematic analysis of sequence and structure relations

(2010) Michael Widmann et al.   BMC BIOCHEMISTRY

α,α′-Dihydroxyketone formation using aromatic and heteroaromatic aldehydes with evolved transketolase enzymes

(2010) James L. Galman et al.   CHEMICAL COMMUNICATIONS

Enzyme-catalyzed C–C bond formation using 2-methyltetrahydrofuran (2-MTHF) as (co)solvent: efficient and bio-based alternative to DMSO and MTBE

(2010) Saravanakumar Shanmuganathan et al.   GREEN CHEMISTRY

The Crystal Structure of Human Transketolase and New Insights into Its Mode of Action

(2010) Lars Mitschke et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure and Reactivity of Bacillus subtilis MenD Catalyzing the First Committed Step in Menaquinone Biosynthesis

(2010) Alice Dawson et al.   JOURNAL OF MOLECULAR BIOLOGY

Non-α-hydroxylated aldehydes with evolved transketolase enzymes

(2010) Armando Cázares et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Structural and functional analysis of Vitamin K2 synthesis protein MenD

(2009) Amit Priyadarshi et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Thiamin diphosphate in biological chemistry: exploitation of diverse thiamin diphosphate-dependent enzymes for asymmetric chemoenzymatic synthesis

(2009) Michael Müller et al.   FEBS Journal

MenD as a versatile catalyst for asymmetric synthesis

(2009) Anja Kurutsch et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Investigation of the carboligase activity of thiamine diphosphate-dependent enzymes using kinetic modeling and NMR spectroscopy

(2009) Mariya Kokova et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Cyclohexane-1,2-dione hydrolase: A new tool to degrade alicyclic compounds

(2009) Sonja Fraas et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Comparative characterisation of thiamin diphosphate-dependent decarboxylases

(2009) Dörte Gocke et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Active-Site Engineering of Benzaldehyde Lyase Shows That a Point Mutation Can Confer Both New Reactivity and Susceptibility to Mechanism-Based Inhibition

(2009) Gabriel S. Brandt et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Multidisciplinary Approach Toward the Rapid and Preparative-Scale Biocatalytic Synthesis of Chiral Amino Alcohols: A Concise Transketolase-/ω-Transaminase-Mediated Synthesis of (2S,3S)-2-Aminopentane-1,3-diol

(2009) Mark E. B. Smith et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Application of a modified Mosher’s method for the determination of enantiomeric ratio and absolute configuration at C-3 of chiral 1,3-dihydroxy ketones

(2009) James L. Galman et al.   TETRAHEDRON-ASYMMETRY

Enhancing and Reversing the Stereoselectivity ofEscherichia coliTransketolaseviaSingle-Point Mutations

(2008) Mark E. B. Smith et al.   ADVANCED SYNTHESIS & CATALYSIS

α,β-Unsaturated Aldehydes as Substrates for Asymmetric CC Bond Forming Reactions with Thiamin Diphosphate (ThDP)-Dependent Enzymes

(2008) Anabel Cosp et al.   ADVANCED SYNTHESIS & CATALYSIS

Rational Protein Design of ThDP-Dependent Enzymes—Engineering Stereoselectivity

(2008) Dörte Gocke et al.   CHEMBIOCHEM

Thiamin Diphosphate Catalysis: Enzymic and Nonenzymic Covalent Intermediates

(2008) Ronald Kluger et al.   CHEMICAL REVIEWS

Directed evolution of transketolase substrate specificity towards an aliphatic aldehyde

(2008) E HIBBERT et al.   JOURNAL OF BIOTECHNOLOGY

Specificity and Reactivity in Menaquinone Biosynthesis: The Structure of Escherichia coli MenD (2-Succinyl-5-Enolpyruvyl-6-Hydroxy-3-Cyclohexadiene-1-Carboxylate Synthase)

(2008) Alice Dawson et al.   JOURNAL OF MOLECULAR BIOLOGY

Enantioselective CC Bond Ligation Using RecombinantEscherichia coli-Whole-Cell Biocatalysts

(2007) Pablo Domínguez de María et al.   ADVANCED SYNTHESIS & CATALYSIS

A microplate-based evaluation of complex denaturation pathways: Structural stability ofEscherichia coli transketolase

(2007) Jean P. Aucamp et al.   BIOTECHNOLOGY AND BIOENGINEERING