Article
Oncology
Seiichiro Ishihara, Hisashi Haga
Summary: Matrix stiffness is critical for cancer progression, regulated by transcription factors. Stiff extracellular matrices trigger mechanotransduction, influencing cancer and stromal cells. YAP/TAZ transcription factors respond to matrix stiffness and play a crucial role.
Article
Multidisciplinary Sciences
Hugh Xiao, Ryan Y. Nguyen, Ryan LaRanger, Erica L. Herzog, Michael Mak
Summary: Cellular interactions with the extracellular matrix play a key role in modulating biological processes, yet the mechanical regulation associated with these interactions is not well characterized. Through a novel image analysis platform, combined with drug studies and quantitative analysis, researchers are able to provide insights into cell mechanics and its implications in a 3D collagen gel.
SCIENTIFIC REPORTS
(2021)
Article
Physiology
Li Liu, Elisabeth Bankell, Catarina Rippe, Bjorn Moren, Karin G. Stenkula, Bengt-Olof Nilsson, Karl Sward
Summary: Myocardin related transcription factors play a key role in smooth muscle cell differentiation, with both anti-inflammatory and pro-inflammatory properties observed in different cell types. The impact of these factors on inflammatory signaling pathways remains complex and cell-type dependent.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Engineering, Biomedical
Yaru Guo, Feng Mei, Ying Huang, Siqin Ma, Yan Wei, Xuehui Zhang, Mingming Xu, Ying He, Boon Chin Heng, Lili Chen, Xuliang Deng
Summary: This study demonstrates that matrix stiffness promotes sprouting of endothelial cells and regulates the formation of tip cells through a specific signaling axis. The findings have significant implications for biomaterial design and treatment of pathological conditions.
BIOACTIVE MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Austin Lai, Peter Thurgood, Charles D. Cox, Chanly Chheang, Karlheinz Peter, Anthony Jaworowski, Khashayar Khoshmanesh, Sara Baratchi
Summary: This study investigates the influence of extracellular matrix (ECM) composition on the mechanosensitivity of the Piezo1 ion channel in response to hemodynamic forces. The presence of ECM proteins does not affect the sensitivity of Piezo1 to shear stress, but different ECM proteins regulate Piezo1 sensitivity depending on the shear stress level. These findings demonstrate that ECM/integrin interactions play a role in Piezo1 mechanosensitivity and provide new insights into how Piezo1 senses shear stress.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Nanoscience & Nanotechnology
Austin Lai, Peter Thurgood, Charles D. Cox, Chanly Chheang, Karlheinz Peter, Anthony Jaworowski, Khashayar Khoshmanesh, Sara Baratchi
Summary: The composition of the extracellular matrix affects the mechanosensitivity of the Piezo1 channel in response to hemodynamic forces. Different ECM proteins can regulate the sensitivity of Piezo1 to shear stress, while integrins play important roles in this process.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Biochemistry & Molecular Biology
Tonia L. Vincent, Oliver McClurg, Linda Troeberg
Summary: The extracellular matrix (ECM) plays a crucial role in tissue homeostasis and disease, acting as a mechano-sensor and mechano-effector in cartilage. It is involved in perceiving and responding to mechanical load, releasing growth factors for cartilage repair and protection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Sebastian E. Amos, Yu Suk Choi
Summary: Metastatic cascade poses a significant challenge to cancer patient survival, with mechanical stimuli and microenvironment playing crucial roles in the metastatic process.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Yijun Zheng, Mitchell K. L. Han, Renping Zhao, Johanna Blass, Jingnan Zhang, Dennis W. Zhou, Jean-Remy Colard-Itte, Damien Dattler, Arzu Colak, Markus Hoth, Andres J. Garcia, Bin Qu, Roland Bennewitz, Nicolas Giuseppone, Aranzazu del Campo
Summary: The study introduces a unique molecular machine that uses light-driven rotatory molecular motor to apply forces at cell-matrix and cell-cell junctions, pulling on cell membrane receptors. These forces have physiologically-relevant magnitude and occur within relevant time scales for mechanotransduction, showing potential for manipulating living cells at the molecular scale.
NATURE COMMUNICATIONS
(2021)
Review
Physiology
Ting Guo, Cindy Wantono, Yuyong Tan, Feihong Deng, Tianying Duan, Deliang Liu
Summary: This article provides a comprehensive view of the role of matrix stiffness in the pathological progression of hepatic disease, particularly liver cirrhosis and carcinoma. The regulators that modulate matrix stiffness, including ECM components, MMPs, and crosslinking modifications, are discussed. The latest advances in the research on matrix mechanics in regulating intercellular signaling and cell phenotype are classified, with a focus on hepatic stellate cells, hepatocytes, and immunocytes. The molecular mechanism of sensing and transducing mechanical signaling is highlighted, and the current progress in targeting ECM stiffness for the treatment of liver disease is detailed.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Inmaculada Navarro-Lerida, Anna M. Aragay, Alejandro Asensio, Catalina Ribas
Summary: All processes in human physiology rely on homeostatic mechanisms, and autophagy is a critical modulator of homeostatic balance. Understanding the regulation and mechanisms of autophagy is of great importance in both protective and detrimental functions. The identification of selective receptors involved in autophagy regulation is an open question and represents a new area of research. G-protein coupled receptors (GPCRs), specifically G alpha q, play a fundamental role in the control of cellular homeostasis and autophagy. Exploring the integration of GPCR-G proteins machinery in response to different stresses could provide insights into new signaling pathways and therapeutic strategies.
Article
Chemistry, Multidisciplinary
Qian Sun, Fang Pei, Man Zhang, Bo Zhang, Ying Jin, Zhihe Zhao, Qiang Wei
Summary: This study focuses on understanding the role of the curved structure in the extracellular matrix (ECM) network in regulating stem cell mechanotransduction. The fabrication of a curved nanofiber network promotes cell bridge formation, leading to cell lineage commitment towards osteogenic differentiation.
Article
Cell Biology
Kathrin Weber, Sven Hey, Pasquale Cervero, Stefan Linder
Summary: Podosomes are highly dynamic actin-rich structures found in various cell types, especially monocytic cells, serving functions such as adhesion, mechanosensing, and extracellular matrix degradation. These functions are enabled by an intricate architecture and the coordinated recruitment of over 300 different components during podosome formation and turnover. Mechanoregulatory processes play a significant role across different stages of the podosome lifecycle, at both individual podosome and sub-and superstructure levels.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Antonios N. Gargalionis, Kostas A. Papavassiliou, Efthimia K. Basdra, Athanasios G. Papavassiliou
Summary: This review explores the role of the mechanistic target of rapamycin (mTOR) in regulating tumor mechanobiology. The mTOR signaling pathway is involved in the regulation of cancer cell growth, invasion, and metastasis through the activation of signaling cascades. The article discusses the association between mTOR signaling components and tumor mechanobiology, as well as mechanisms related to the therapeutic efficacy of antitumor drugs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Daphne O. Asgeirsson, Michael G. Christiansen, Thomas Valentin, Luca Somm, Nima Mirkhani, Amin Hosseini Nami, Vahid Hosseini, Simone Schuerle
Summary: The study presents methods for spatiotemporally probing and manipulating extracellular matrix networks at a scale relevant to cells using magnetic microprobes (mu Rods). Techniques leverage 3D magnetic field generation, physical modeling, and image analysis to examine and apply mechanical stimuli to fibrous collagen matrices. The study determined shear moduli and modeled the effects of proximity to rigid surfaces and local fiber densification, demonstrating 3D actuation and pose extraction of fluorescently labelled mu Rods in response to magnetic torques.
Article
Dentistry, Oral Surgery & Medicine
T. Kouskoura, Y. El Fersioui, M. Angelini, D. Graf, C. Katsaros, M. Chiquet
JOURNAL OF DENTAL RESEARCH
(2016)
Article
Cell Biology
Kim S. Midwood, Matthias Chiquet, Richard P. Tucker, Gertraud Orend
JOURNAL OF CELL SCIENCE
(2016)
Article
Physiology
Matthias Chiquet, Susan Blumer, Manuela Angelini, Thimios A. Mitsiadis, Christos Katsaros
FRONTIERS IN PHYSIOLOGY
(2016)
Article
Dentistry, Oral Surgery & Medicine
Maria B. Asparuhova, Deniz Kiryak, Meizi Eliezer, Deyan Mihov, Anton Sculean
JOURNAL OF PERIODONTAL RESEARCH
(2019)
Article
Physiology
Joel Beyeler, Christos Katsaros, Matthias Chiquet
FRONTIERS IN PHYSIOLOGY
(2019)
Article
Dentistry, Oral Surgery & Medicine
Maria B. Asparuhova, Vivianne Chappuis, Alexandra Stahli, Daniel Buser, Anton Sculean
CLINICAL ORAL INVESTIGATIONS
(2020)
Article
Chemistry, Physical
Cristina Nica, Zhikai Lin, Anton Sculean, Maria B. Asparuhova
Article
Dentistry, Oral Surgery & Medicine
Ludovica Parisi, Daniel Buser, Vivianne Chappuis, Maria B. Asparuhova
Summary: The study aimed to investigate the effects of bone-conditioned medium (BCM) on the biological characteristics of deproteinized bovine bone mineral (DBBM) within a clinically relevant short-term period. The results showed that BCM extracted within 20 minutes or 24 hours significantly enhanced the adhesive, proliferative, and osteogenic differentiation properties of cells grown on BCM-coated DBBM.
CLINICAL ORAL INVESTIGATIONS
(2021)
Article
Chemistry, Physical
Zhikai Lin, Cristina Nica, Anton Sculean, Maria B. Asparuhova
Editorial Material
Immunology
Matthias Chiquet
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Immunology
Thomas Imhof, Anamaria Balic, Juliane Heilig, Ruth Chiquet-Ehrismann, Matthias Chiquet, Anja Niehoff, Bent Brachvogel, Irma Thesleff, Manuel Koch
Summary: The continuously growing mouse incisor provides a model for studying stem cell regulation and organ renewal. Deficiency in tenascin-W/TNN in mice leads to reduced body weight and lifespan, major defects in periodontal tissues, and decreased hedgehog signaling in mesenchymal stem cells of the incisor. This deficiency affects periodontal remodeling, increases nerve fiber branching, reduces food intake through periodontal pain, and impacts the rate of incisor renewal and neurovascular sonic hedgehog secretion.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Maria B. Asparuhova, Alexandra Stahli, Kevin Guldener, Anton Sculean
Summary: The novel volume-stable collagen matrix was found to positively impact cellular migration and adhesion, growth factor adsorption and release, as well as the dynamics of the hemostatic system, ultimately facilitating the progression of soft tissue healing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Zhikai Lin, Cristina Nica, Anton Sculean, Maria B. Asparuhova
Summary: Recent research has shown that HADM and CCM have a favorable influence on the migration, adhesion, and proliferation of osteoprogenitor cells, as well as promoting osteogenic differentiation. Coating the collagen-based matrices with EMD or rBMP-2 can significantly enhance osteogenic differentiation on all matrices.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Maria B. Asparuhova, Dominic Riedwyl, Ryo Aizawa, Clemens Raabe, Emilio Couso-Queiruga, Vivianne Chappuis
Summary: Healing after tooth extraction involves reparative processes affecting both alveolar bone and soft tissues. This study investigated the activation of molecular signals during the healing process and found that extraction socket soft tissue (ESsT) showed a regenerative advantage compared to connective tissue graft (CTG). Mechanical stimulation and growth factors such as transforming growth factor-beta 1 (TGF-beta 1) and insulin-like growth factor-1 (IGF-1) were found to influence the osteogenic potential of ESsT cells. These findings suggest the potential clinical use of ESsT as a graft in dental implant placement surgeries.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Dentistry, Oral Surgery & Medicine
Maria B. Asparuhova, Jordi Caballe-Serrano, Daniel Buser, Vivianne Chappuis
INTERNATIONAL JOURNAL OF ORAL SCIENCE
(2018)