Article
Cell Biology
Maria P. Clemente-Olivo, Miguel Hernandez-Quiles, Rinske Sparrius, Miesje M. van der Stoel, Vera Janssen, Jayron J. Habibe, Janny van den Burg, Aldo Jongejan, Paula Alcaraz-Sobrevals, Robert van Es, Harmjan Vos, Eric Kalkhoven, Carlie J. M. de Vries
Summary: This study found that FHL2 is expressed in pre-adipocytes and needs to be downregulated during the early stages of adipogenesis. Overexpression of FHL2 significantly inhibits adipocyte differentiation and interacts with NFAT5 to mediate this inhibition. FHL2 expression increases with aging, potentially affecting mesenchymal stem cell differentiation, particularly adipocyte differentiation.
CELLULAR SIGNALLING
(2023)
Article
Cell Biology
Thi An Vu, Ingrid Lema, Imene Hani, Lydie Cheval, Laura Atger-Lallier, Vilayvane Souvannarath, Julie Perrot, Melanie Souvanheuane, Yannick Marie, Sylvie Fabrega, Anne Blanchard, Jerome Bouligand, Peter Kamenicky, Gilles Crambert, Laetitia Martinerie, Marc Lombes, Say Viengchareun
Summary: Under hypertonicity, miR-324-5p and miR-30c-2-3p are recruited to regulate MR expression and signaling, affecting renal function and sodium retention.
Article
Immunology
Khalid Muhammad, Delicia Xavier, Stefan Klein-Hessling, Muhammad Azeem, Tabea Rauschenberger, Krisna Murti, Andris Avots, Matthias Goebeler, Matthias Klein, Tobias Bopp, Malte Sielaff, Stefan Tenzer, Sigrid Moeckel, Jose Aramburu, Cristina Lopez-Rodriguez, Andreas Kerstan, Edgar Serfling
Summary: NFAT5 is a key transcription factor in keratinocytes that plays a crucial role in regulating the expression of matrix proteases, which in turn impacts skin development and epidermal integrity in adults.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Yen-Yi Zhen, Chien-Hsing Wu, Hung-Chun Chen, Eddy Essen Chang, Jia-Jung Lee, Wei-Yu Chen, Jer-Ming Chang, Pei-Yun Tseng, Yue-Fang Wang, Chi-Chih Hung
Summary: The renal epithelial barrier plays multiple roles in maintaining body fluids, electrolyte balance, and urine production. This study shows that LMO7 protein is expressed in renal tubule epithelial cells and has a role in withstanding osmotic pressure. The findings suggest that LMO7 regulates FAK activation to maintain the integrity of the renal epithelial barrier.
Editorial Material
Genetics & Heredity
Ivona Aksentijevich
Summary: Immune responses require a delicate balance - too weak or too strong a response can cause issues. Spleen tyrosine kinase plays a crucial role in multiple signaling pathways, and its gain-of-function alterations can lead to various problems, including hypogammaglobulinemia and predisposition to B cell lymphoma.
Review
Biochemistry & Molecular Biology
Ashwin K. Chetty, Byung Hak Ha, Titus J. Boggon
Summary: This review focuses on the signaling pathways mediated by Rho family small GTPases and the specific roles of type II PAKs. The selectivity of Rho GTPases for PAK family members and the molecular basis for substrate recognition and regulation of signaling are discussed. The cross talk between Rho family small GTPases and the involvement of PAKs in diseases are also addressed.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Leszek A. Kleczkowski, Abir U. Igamberdiev
Summary: Free magnesium (Mg2+) functions as a signal of adenylate (ATP+ADP+AMP) status in cells, controlled by the equilibrium of adenylate kinase (AK) and membrane-bound adenylate- and Mg2+-translocators. Changes in [Mg2+] impact enzyme activities, metabolism, and processes like photosynthesis, under normal and stress conditions. Magnesium signaling is likely widespread in all eukaryotic and prokaryotic organisms due to the ubiquitous nature of AK and its role in adenylate equilibration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Do Hyeong Gwon, Song I. Kim, Seoung Hun Lee, Chan Noh, Yeojung Kim, Sangwon Yun, Won Hyung Lee, Jun Young Oh, Dong Woon Kim, Jinpyo Hong, Sun Yeul Lee
Summary: NFAT5 deficiency may alleviate formalin-induced inflammatory pain by upregulating mTOR expression and downregulating its downstream factors in spinal dorsal neurons.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Lauren C. Kinkead, Samantha J. Krysa, Lee-Ann H. Allen
Summary: This study investigated the contributions of ERK and p38 MAP kinase, PI3K, AKT, and NF-kappa B to neutrophil survival. The results showed that p38 alpha, PI3K alpha, and NF-kappa B play important roles in the delayed apoptosis of infected cells.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Qian Wang, Chenqi Tao, Abdul Hannan, Sungtae Yoon, Xuanyu Min, John Peregrin, Xiuxia Qu, Hongge Li, Honglian Yu, Jean Zhao, Xin Zhang
Summary: The patterning of epithelial buds in lacrimal gland development is determined by the interplay between PI3K, Ras, and ERK signaling pathways. PI3K is activated by both IGF and FGF signaling, and also promotes ERK signaling through direct interaction with Ras. Inhibition of EGF signaling is essential for induction of lacrimal gland buds, and the cross-talk among FGF, IGF, and EGF signaling is mediated by PI3K, MAPK, and mTOR.
Article
Biology
Gary K. L. Chan, Samantha Maisel, Yeonjoo C. Hwang, Bryan C. Pascual, Rebecca R. B. Wolber, Phuong Vu, Krushna C. Patra, Mehdi Bouhaddou, Heidi L. Kenerson, Huat C. Lim, Donald Long, Raymond S. Yeung, Praveen Sethupathy, Danielle L. Swaney, Nevan J. Krogan, Rigney E. Turnham, Kimberly J. Riehle, John D. Scott, Nabeel Bardeesy, John D. Gordan
Summary: Genetic alterations that activate protein kinase A (PKA) are found in many tumor types. The downstream oncogenic signaling mechanisms of PKA activation in human cancer were explored using phosphoproteomics and kinase activity profiling. Two signaling networks, RAS/MAPK components and an AURKA/GSK3 sub-network, were identified, both affecting MYC oncoproteins. Primary mechanism of PKA effects on MYC was found to be translation, which could be blocked with the eIF4A inhibitor zotatifin, suggesting a potential treatment strategy for PKA-driven cancers.
Review
Biochemistry & Molecular Biology
Manali Tilak, Jennifer Holborn, Laura A. New, Jasmin Lalonde, Nina Jones
Summary: Glioblastoma multiforme (GBM) is a deadly cancer with limited response to existing therapies. Subtypes of GBM with distinct genetic signatures show aberrant activation of signal transduction pathways. Current research focuses on understanding these molecular alterations to develop more efficient targeted therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Huifen Zhen, Yarong Yao, Haibo Yang
Summary: In this study, the role of SAFB2 in breast cancer and its underlying mechanism was investigated. It was found that SAFB2 was downregulated while NFAT5 was highly expressed in breast cancer cell lines. Overexpression of SAFB2 inhibited proliferation, migration, and invasion, and induced apoptosis of breast cancer cells. SAFB2 interacted with NFAT5 mRNA and destabilized it. Overexpression of NFAT5 counteracted the anti-proliferative, anti-metastatic, and pro-apoptotic effects of SAFB2. Mechanistically, SAFB2 inhibited the Wnt/beta-catenin signaling pathway, which was partially restored by NFAT5.
MOLECULAR BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Guangze Zhao, Sana Aghakeshmiri, Yankuan T. Chen, Huifang M. Zhang, Fione Yip, Decheng Yang
Summary: NFAT5 is sensitive to both osmotic and non-osmotic stress signals, playing a protective role against detrimental effects. Dysregulation of NFAT5 expression may cause pathological conditions, dictated by target gene regulation and signaling pathway activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Yatian Li, Zhenyue Gao, Yuhong Wang, Bo Pang, Binbin Zhang, Ruxin Hu, Yuqing Wang, Chao Liu, Xuebin Zhang, Jingxuan Yang, Mei Mei, Yongzhi Wang, Xuan Zhou, Min Li, Yu Ren
Summary: This study found that NFAT5 lysine methylation is a determinant of the response to temozolomide (TMZ) therapy. Activation of EGFR induces phosphorylated EZH2 (Ser21) binding and triggers NFAT5 methylation at K668. Methylation prevents NFAT5 degradation and cytosol localization restriction, leading to NFAT5 protein stabilization, nuclear accumulation and activation. Inhibition of NFAT5 K668 methylation can improve TMZ efficacy in xenograft and patient-derived xenograft (PDX) models.
NATURE COMMUNICATIONS
(2023)
Review
Hematology
Charlene V. Chabata, James W. Frederiksen, Bruce A. Sullenger, Ruwan Gunaratne
CURRENT OPINION IN HEMATOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Cheng-Wei Lin, Chieh-Wen Lo, Chia-Ni Tsai, Ting-Chun Pan, Pin-Yin Chen, Ming-Jiun Yu
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Virology
Shih-Chin Hsu, Chia-Ni Tsai, Kuan-Ying Lee, Ting-Chun Pan, Chieh-Wen Lo, Ming-Jiun Yu
JOURNAL OF VIROLOGY
(2018)
Article
Biochemical Research Methods
Ting-Chun Pan, Chieh-Wen Lo, Weng Man Chong, Chia-Ni Tsai, Kuan-Ying Lee, Pin-Yin Chen, Jung-Chi Liao, Ming-Jiun Yu
JOURNAL OF PROTEOME RESEARCH
(2019)
Article
Virology
Chia-Ni Tsai, Ting-Chun Pan, Cho-Han Chiang, Chun-Chiao Yu, Shih-Han Su, Ming-Jiun Yu
JOURNAL OF VIROLOGY
(2019)
Article
Cell Biology
Lucie Yammine, Aniela Zablocki, William Baron, Fabiola Terzi, Morgan Gallazzini
Article
Physiology
Shu-Ting Lin, Chia-Ching Ma, Kuang-Ting Kuo, Yin-Fang Su, Wei-Ling Wang, Tzu-Hsien Chan, Shih-Han Su, Shih-Che Weng, Chian-Huei Yang, Shuei-Liong Lin, Ming-Jiun Yu
FRONTIERS IN PHYSIOLOGY
(2019)
Article
Virology
Cho-Han Chiang, Yen-Ling Lai, Yu-Ning Huang, Chun-Chiao Yu, Christine C. Lu, Guann-Yi Yu, Ming-Jiun Yu
JOURNAL OF VIROLOGY
(2020)
Article
Medicine, Research & Experimental
Mohamad Zaidan, Martine Burtin, Jitao David Zhang, Thomas Blanc, Pauline Barre, Serge Garbay, Clement Nguyen, Florence Vasseur, Lucie Yammine, Serena Germano, Laura Badi, Marie-Claire Gubler, Morgan Gallazzini, Gerard Friedlander, Marco Pontoglio, Fabiola Terzi
Article
Biochemistry & Molecular Biology
Charlene V. Chabata, James W. Frederiksen, Lyra B. Olson, Ibtehaj A. Naqvi, Sharon E. Hall, Ruwan Gunaratne, Bryan D. Kraft, Loretta G. Que, Lingye Chen, Bruce A. Sullenger
Summary: This study explores the combination of anticoagulant aptamers with unfractionated heparin (UFH) to address limitations of UFH use. The combination significantly enhances anticoagulation, supports blood circulation, and can be rapidly reversed. This finding has important implications for improving anticoagulant therapy in acute care patients.
NUCLEIC ACID THERAPEUTICS
(2022)
Article
Virology
Chun-Chiao Yu, Pei-Chen Lin, Cho-Han Chiang, Shu-Tang Jen, Yen-Ling Lai, Shih-Chin Hsu, Lee-Chiang Lo, Jing-Jer Lin, Nei-Li Chan, Ming-Jiun Yu
Summary: In this study, we discovered for the first time that the ATP-binding domain of NS3 is involved in NS5A phosphorylation. In vitro assays showed that casein kinase I alpha is a very potent kinase responsible for NS5A phosphorylation at serines 225, 232, and 235. Our data suggest that ATP binding by NS3 probably results in conformational changes that recruit casein kinase I alpha to phosphorylate NS5A, initially at S225 and subsequently at S232 and S235. Our discovery reveals intricate requirements of the structural integrity of NS3 for NS5A hyperphosphorylation and HCV replication.
JOURNAL OF VIROLOGY
(2022)
Article
Cell Biology
Eloise Marques, Maraiza Alves Teixeira, Clement Nguyen, Fabiola Terzi, Morgan Gallazzini
Summary: The Lcn2 gene plays a critical role in preventing mitochondrial dysfunction in kidney injury. The study found that LCN2 expression decreases mitochondrial mass and function, causing mitochondrial fragmentation, and the mechanism is related to mTOR activity.
Article
Physiology
Kit Yee Wong, Wei-Ling Wang, Shih-Han Su, Chin-Fu Liu, Ming-Jiun Yu
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2020)
Article
Physiology
Wei-Ling Wang, Shih-Han Su, Kit Yee Wong, Chan-Wei Yang, Chin-Fu Liu, Ming-Jiun Yu
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2020)
Article
Physiology
Kuang-Ting Kuo, Chan-Wei Yang, Ming-Jiun Yu
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2018)