Review
Biochemistry & Molecular Biology
Anton Sergeevich Shakhov, Aleksandra Sergeevna Churkina, Anatoly Alekseevich Kotlobay, Irina Borisovna Alieva
Summary: This review summarizes information about the specific features of the centrosome and its relationship with the cell function of highly specialized cells. The participation of the centrosome in the functional activity of these cells, as well as its role as a universal regulatory and distribution center, controlling the dynamic morphology of animal cells, is discussed. The direct involvement of the centrosome in the performance of functions by specialized cells is also highlighted.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Ophthalmology
Mohit Parekh, Alessandro Ruzza, Paola Gallon, Diego Ponzin, Sajjad Ahmad, Stefano Ferrari
Summary: This study compared the difference in various endothelial graft preparation methods and endothelial cell culture between tissues preserved in serum-based and synthetic medium. The results indicated that the complete synthetic organ culture series is safe and advantageous for advanced endothelial keratoplasty graft preparation procedures and HCEnC culture as it is free from animal or animal-derived products.
ACTA OPHTHALMOLOGICA
(2021)
Article
Ophthalmology
Agathe Hribek, Thomas Clahsen, Jens Horstmann, Sebastian Siebelmann, Niklas Loreck, Ludwig M. Heindl, Bjorn O. Bachmann, Claus Cursiefen, Mario Matthaei
Summary: This study aimed to assess the correlation of CEC density with alterations of collagen composition of DM in FECD patients. The presence of a geographic pattern fibrillar layer in DEC was unveiled, which can be imaged by slit-lamp biomicroscopy, and the CEC density in the fibrillar layer region significantly decreased.
AMERICAN JOURNAL OF OPHTHALMOLOGY
(2021)
Article
Medicine, General & Internal
Usanee Reinprayoon, Monthira Jermjutitham, Ngamjit Kasetsuwan
Summary: This study found that with the increasing severity of Fuchs' endothelial corneal dystrophy (FECD), the rate of endothelial cell loss increased and the corneal biomechanical properties changed. In the advanced stage, the corneas significantly altered their viscoelastic behavior by decreasing resistance.
FRONTIERS IN MEDICINE
(2021)
Article
Ophthalmology
Diego G. Ogando, Rajalekshmy Shyam, Edward T. Kim, Yen-Chiao Wang, Chia-Yang Liu, Joseph A. Bonanno
Summary: The study successfully replicated the corneal edema phenotype of the conventional Slc4a11 KO model using an inducible KO model, validating its effectiveness and providing a tool for further research on the sequence of cellular events.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Review
Cell Biology
Mostafa Khalili, Maryam Asadi, Houman Kahroba, Mohammad Reza Soleyman, Helder Andre, Effat Alizadeh
Summary: The cornea is a transparent avascular tissue that focuses light on the retina. Restoring vision in patients with corneal endothelium dysfunction often requires corneal transplantation, but donor limitations pose challenges. An emerging alternative to transplantation is corneal endothelium tissue engineering, which uses scaffold-based and scaffold-free methods.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Cell & Tissue Engineering
Fatma Zehra Erkoc-Biradli, Alp Ozgun, Meftune Ozgen Ozturk-Oncel, Merve Marcali, Caglar Elbuken, Osman Bulut, Rifat Rasier, Bora Garipcan
Summary: The study demonstrated that designing cell substrates mimicking the native CE microenvironment promotes the formation of CE cell monolayers with desirable cell density.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Review
Physiology
Dietmar Vestweber
Summary: VE-PTP, predominantly expressed in vascular endothelial cells, regulates vascular functions by controlling its substrates and shows therapeutic potential in various pathophysiological settings.
Article
Ophthalmology
Sheldon R. Gordon, Samantha Dimovski, Nicholas Kocik
Summary: The study investigated the binding of lectin soybean agglutinin (SBA) to the corneal endothelium cell surface during organ culture using Sprague-Dawley rats. The results showed that SBA binding occurred in a punctate pattern after 48 hours of organ culture and only nectin-3 was responsible for this binding. The emergence of nectin-3 around the cell periphery in organ culture suggests its necessary role in maintaining monolayer integrity and barrier function.
EXPERIMENTAL EYE RESEARCH
(2022)
Article
Ophthalmology
Angela Gomez, Andres Serrano, Enrique Salero, Arianna Tovar, Guillermo Amescua, Anat Galor, Robert W. Keane, Juan Pablo de Rivero Vaccari, Alfonso L. Sabater
Summary: Chronic corneal endothelial cell loss, induced by pro-inflammatory cytokines like TNF-alpha and IFN-gamma, is associated with inflammasome activation and subsequent pyroptosis. Inhibition of inflammasome presents a promising therapeutic target for the treatment of chronic CEC loss.
EXPERIMENTAL EYE RESEARCH
(2021)
Review
Ophthalmology
Marianne O. Price, Jodhbir S. Mehta, Ula Jurkunas, Francis W. Price
Summary: The cornea protects and focuses incoming light for the eye. Precise control of corneal hydration by endothelial cells is vital for transparency and visual health. Advances in corneal transplantation offer new options for treating dysfunction, while research into alternative treatments continues.
PROGRESS IN RETINAL AND EYE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Seongjun So, Yoonkyung Park, Soon Suk Kang, Jongsuk Han, Jeong Hye Sunwoo, Whanseo Lee, Jin Kim, Eun Ah Ye, Jae Yong Kim, Hungwon Tchah, Eunju Kang, Hun Lee
Summary: Corneal endothelial cells (CECs) cannot proliferate or recover, causing decreased cell density and loss of pump/barrier function. The study investigated the efficacy and safety of transplanting induced pluripotent stem cell (iPSC)-derived CECs into a corneal endothelial dysfunction (CED) rabbit model. Results showed successful differentiation of iPSCs into CECs and improved corneal transparency after transplantation. iPSC-derived CECs may be a promising cellular resource for treating CED.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell & Tissue Engineering
Ingrida Smeringaiova, Tor Paaske Utheim, Katerina Jirsova
Summary: The corneal endothelium is crucial for maintaining corneal transparency, and its dysfunction is currently challenging to treat. Cadaveric corneas appear to be the best cell source for graft preparation, despite the limited natural proliferative capacity of native endothelial cells and their tendency to undergo phenotype changes under ex vivo culture conditions.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Ophthalmology
Chunxiao Dong, Dulei Zou, Haoyun Duan, Xiangyue Hu, Qingjun Zhou, Weiyun Shi, Zongyi Li
Summary: Stem cell therapy using retinal pigment epithelium (RPE) cells holds promise for treating corneal endothelial dysfunction. The study showed that RPE cells have high structural and functional similarities to corneal endothelial cells (CECs). Injection of primary RPE cells and human embryonic stem cell (hESC)-derived RPE cells effectively restored corneal clarity and thickness in rabbits. This study is the first to demonstrate the feasibility of RPE cells as an equivalent substitute for CECs.
Article
Biochemistry & Molecular Biology
Xin Wang, Chunxiao Dong, Qingjun Zhou, Haoyun Duan, Dulei Zou, Yajie Gong, Bochao Ma, Zongyi Li, Weiyun Shi
Summary: The study suggests that PARP1 inhibition can protect corneal endothelium from UVA-induced damage and exert anti-apoptotic effects by controlling the phosphorylation levels of JNK1/2 and p38 MAPK.
