Review
Immunology
Yanran Li, Qiyuan Yang, Dongjie Cai, Hongrui Guo, Jing Fang, Hengmin Cui, Liping Gou, Junliang Deng, Zhisheng Wang, Zhicai Zuo
Summary: Resistin, initially proposed as a link between obesity and diabetes in mice, is considered a pro-inflammatory molecule expressed in immune cells in humans, playing a regulatory role in chronic inflammatory diseases, metabolic diseases, infectious diseases, and cancers. However, increasing evidence shows that it functions as a host defense peptide with wide-spectrum anti-microbial activity and immune modulation, limited microbial product-induced inflammation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
Jianhong Ouyang, Yiyun Zhu, Weijing Hao, Xia Wang, Huaixin Yang, Xinyi Deng, Tingting Feng, Yong Huang, Haining Yu, Yipeng Wang
Summary: In this study, three host defense peptides (HDPs) exhibited potent and broad-spectrum antimicrobial activity against aquatic pathogenic bacteria and effectively modulated anti-infective immune responses in largemouth bass. They also showed potential in combating biofilm-related infections and significantly enhancing the survival rate of bacteria-challenged largemouth bass while decreasing bacterial resistance compared to conventional antibiotics. These results suggest the promising use of these HDPs as novel anti-infective agents in largemouth bass culture.
Article
Engineering, Environmental
Jian Lu, Yuxuan Zhang, Jun Wu, Jianhua Wang
Summary: This study investigated the impact of antimicrobial peptide intervention on antimicrobial resistance in aquaculture by analyzing the abundance of antibiotic resistance genes (ARGs) in various samples. The results showed that cecropin treatment significantly reduced the absolute abundance of ARGs, while antibiotic treatment had the opposite effect. Network analysis revealed that cecropin treatment could decrease the occurrence of multi-drug resistant bacteria and the correlation between genera and ARGs.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Microbiology
Frank R. DeLeo, Adeline R. Porter, Scott D. Kobayashi, Brett Freedman, Mingju Hao, Jianping Jiang, Yi-Tsung Lin, Barry N. Kreiswirth, Liang Chen, Joanna B. Goldberg
Summary: Klebsiella pneumoniae is a significant cause of healthcare-associated infections worldwide, with different strains causing infections in individuals with comorbidities and healthy individuals. The emergence of strains with both multidrug resistance and hypervirulence complicates the understanding of the immune response against this pathogen.
Review
Infectious Diseases
Isabel Tobin, Guolong Zhang
Summary: The rise of antimicrobial resistance has led to an urgent need for antibiotic alternatives in disease control and prevention. Host defense peptides (HDPs), with their antimicrobial and immunomodulatory properties, offer a promising solution to treating infections without the risk of developing resistance. Polyphenols, natural compounds found in plants, have been found to stimulate HDP synthesis and show potential as antibiotic alternatives.
Review
Pharmacology & Pharmacy
Hashem Etayash, Robert E. W. Hancock
Summary: Amphiphilic antimicrobial polymers and surface engineered polymeric-brush-tethered HDP are promising strategies that aim to overcome limitations of HDPs, enhance physicochemical properties and therapeutic performance, and address unmet therapeutic needs.
Review
Infectious Diseases
Patrick R. Lennard, Pieter S. Hiemstra, Peter H. Nibbering
Summary: The combination of host defence peptides (HDPs) and antibiotics shows promise in the treatment of antimicrobial resistant (AMR) bacterial infections. Despite biases in the current literature, studies suggest that effective combinations of HDPs and antibiotics hold potential for future treatment of AMR infections.
Article
Biochemistry & Molecular Biology
Aaron P. Decker, Abraham F. Mechesso, Guangshun Wang
Summary: Unlike the alpha-helical and beta-sheet antimicrobial peptides (AMPs), amino acid-rich AMPs have limited knowledge. This article conducted a systematic study on rich AMPs (>25%) from different life kingdoms using the Antimicrobial Peptide Database (APD) and program R. Of the 3425 peptides analyzed, 724 rich AMPs were identified. The distribution of rich AMPs differs among animals, bacteria, and plants. Certain amino acids are frequently observed in rich AMPs, while others are occasionally observed or not yet found. These findings can guide peptide design and rich AMPs show promise as future antibiotics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Zhuo Deng, Wentao Lyu, Guolong Zhang
Summary: Enhancing the synthesis of endogenous host defense peptides (HDPs) has emerged as a novel antibiotic-free approach to infectious disease control and prevention. In this study, a high throughput screening assay was used to identify HDP inducers specifically for poultry. Two compounds, entinostat and tucidinostat, were found to effectively promote HDP gene expression in chicken macrophages and intestinal tissue without triggering an inflammatory response.
Article
Chemistry, Multidisciplinary
Zhongqian Qiao, Wenjing Zhang, Yueming Wu, Weinan Jiang, Ning Shao, Jiayang Xie, Guixue Xia, Qimeng Chen, Zhefeng Liu, Jingcheng Zou, Jiawei Gu, Shifang Luan, Haodong Lin, Runhui Liu
Summary: Wound infections are a significant health issue caused by the invasion and spread of pathogens in wound sites. However, antibiotic resistance, particularly in MRSA, has made it challenging to treat these infections. To address MRSA-infected wounds, an antibacterial peptide polymer-loaded hyaluronic acid (HA) hydrogel (Gel-HA@P) has been developed. This design mimics host defense peptides, exhibiting potent antibacterial activity, low susceptibility to drug resistance, and good stability. Gel-HA@P demonstrates effective activity against MRSA, low toxicity, and promotes wound healing. This strategy provides an antibiotic-free approach for the healing of MRSA-infected wounds.
SCIENCE CHINA-CHEMISTRY
(2023)
Article
Engineering, Environmental
Ming-Kang Jin, Qi Zhang, Wen-Lu Zhao, Zhi-Heng Li, Hai-Feng Qian, Xiao-Ru Yang, Yong-Guan Zhu, Hui-Jun Liu
Summary: The study found that fluoroquinolone antibiotics have toxic effects on soil animals, including activating defense responses, altering gut microbiota, and increasing the risk of antibiotic resistance genes.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Wentao Lyu, Dehui Mi, Paige N. Vinson, Yingping Xiao, Guolong Zhang
Summary: The study aimed to identify small-molecule compounds that can induce the synthesis of endogenous HDPs as novel antimicrobial agents. Through high-throughput screening, 110 compounds were identified, among which mocetinostat showed strong ability to enhance HDP gene expression and the antibacterial activity of chicken macrophages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Pengxiang Li, Yifang Cui, Fangfang Guo, Jiahui Guo, Xiaoya Cao, Jun Lin, Baoan Ding, Fuzhou Xu
Summary: This study aimed to characterize the chicken host defense peptides (HDPs) responses to Campylobacter jejuni (C. jejuni) infection. In vitro experiments showed that C. jejuni infection suppressed the expression of certain HDPs but increased the expression of others. In vivo experiments in chickens demonstrated that C. jejuni infection initially up-regulated the expression of HDPs, but this expression decreased over time. The findings suggest that C. jejuni evades the chicken immune system by suppressing the expression of chicken HDPs.
VETERINARY MICROBIOLOGY
(2023)
Article
Microbiology
Darya V. Poshvina, Diana S. Dilbaryan, Alexey S. Vasilchenko
Summary: Antibiotic resistance is a significant issue in the medical field, and Staphylococcus aureus poses a serious threat as it is responsible for many infections and has high mortality rates. We studied the gene expression of gausemycin A-resistant S. aureus and found that changes in the cell wall and cell membrane are crucial for bacterial resistance. In the late-exponential phase, genes involved in cell wall turnover, membrane charge, phospholipid metabolism, stress response, and proteolytic system showed increased expression, while in the stationary phase, genes involved in phospholipid metabolism and proteolytic system showed decreased expression.
Article
Chemistry, Physical
Tanya Verma, Anchal Aggarwal, Shikha Singh, Sumit Sharma, Saurabh Jyoti Sarma
Summary: The discovery of antibiotics has significantly improved the treatment of infectious diseases and increased human life expectancy. However, the development of antibiotic resistance by microorganisms remains a major concern. The sensitivity and resistivity of bacteria play a crucial role in the effectiveness of antibiotics.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Cell Biology
Rune Troelsgaard Pedersen, Thomas Kruse, Jakob Nilsson, Vibe H. Oestergaard, Michael Lisby
JOURNAL OF CELL BIOLOGY
(2015)
Article
Biochemistry & Molecular Biology
Emil Peter Thrane Hertz, Thomas Kruse, Norman E. Davey, Blanca Lopez-Mendez, Jon Otti Sigurdsson, Guillermo Montoya, Jesper V. Olsen, Jakob Nilsson
Letter
Cell Biology
Jamin B. Hein, Emil P. T. Hertz, Dimitriya H. Garvanska, Thomas Kruse, Jakob Nilsson
NATURE CELL BIOLOGY
(2017)
Article
Multidisciplinary Sciences
Gang Zhang, Thomas Kruse, Blanca Lopez-Mendez, Kathrine Beck Sylvestersen, Dimitriya H. Garvanska, Simone Schopper, Michael Lund Nielsen, Jakob Nilsson
NATURE COMMUNICATIONS
(2017)
Article
Biochemistry & Molecular Biology
Thomas Kruse, Marie Sofie Yoo Larsen, Garry G. Sedgwick, Jon Otti Sigurdsson, Werner Streicher, Jesper V. Olsen, Jakob Nilsson
Article
Infectious Diseases
Anders S. Andersen, Dorthe Sandvang, Kirk M. Schnorr, Thomas Kruse, Soren Neve, Bo Joergensen, Tonny Karlsmark, Karen A. Krogfelt
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2010)
Article
Cell Biology
Thomas Kruse, Gang Zhang, Marie Sofie Yoo Larsen, Tiziana Lischetti, Werner Streicher, Tine Kragh Nielsen, Sara Petersen Bjorn, Jakob Nilsson
JOURNAL OF CELL SCIENCE
(2013)
Article
Multidisciplinary Sciences
Karina T. Simonsen, Gorm Nielsen, Janni Vester Bjerrum, Thomas Kruse, Birgitte H. Kallipolitis, Jakob Moller-Jensen
Article
Multidisciplinary Sciences
Tanja Schneider, Thomas Kruse, Reinhard Wimmer, Imke Wiedemann, Vera Sass, Ulrike Pag, Andrea Jansen, Allan K. Nielsen, Per H. Mygind, Dorotea S. Ravents, Soren Neve, Birthe Ravn, Alexandre M. J. J. Bonvin, Leonardo De Maria, Anders S. Andersen, Lora K. Gammelgaard, Hans-Georg Sahl, Hans-Henrik Kristensen
Article
Biochemistry & Molecular Biology
Thomas Kruse, Sebastian Peter Gnosa, Isha Nasa, Dimitriya Hristoforova Garvanska, Jamin B. Hein, Hieu Nguyen, Jacob Samsoe-Petersen, Blanca Lopez-Mendez, Emil Peter Thrane Hertz, Jeanette Schwarz, Hanna Sofia Pena, Denise Nikodemus, Marie Kveiborg, Arminja N. Kettenbach, Jakob Nilsson
Article
Biochemistry & Molecular Biology
Yumi Ueki, Michael A. Hadders, Melanie B. Weisser, Isha Nasa, Paula Sotelo-Parrilla, Lauren E. Cressey, Tanmay Gupta, Emil P. T. Hertz, Thomas Kruse, Guillermo Montoya, A. Arockia Jeyaprakash, Arminja Kettenbach, Susanne M. A. Lens, Jakob Nilsson
Summary: The study reveals the structure and function of the PP2A-B56-hSgo1 complex during mitosis, highlighting the essential role of a conserved pocket on the B56 regulatory subunit for hSgo1 binding and cohesion protection. Additionally, it shows that hSgo1 inhibits the binding of PP2A-B56 substrates and that PP2A-B56 dephosphorylates Cdk1 sites on hSgo1 to regulate cohesin interactions. Overall, the research provides important insights into cohesion protection during mitosis.
Article
Biochemistry & Molecular Biology
Robert F. Shearer, Dimitris Typas, Fabian Coscia, Sofie Schovsbo, Thomas Kruse, Andreas Mund, Niels Mailand
Summary: Ubiquitin conjugation to substrate proteins regulates cellular processes and the K27 linkage type is essential for human cell proliferation. K27-linked ubiquitylation predominantly occurs in the nucleus and its disruption impairs nuclear ubiquitylation dynamics and cell cycle progression. K27-linked ubiquitylation supports cell fitness by facilitating p97-dependent processing of ubiquitylated nuclear proteins.
Article
Biochemistry & Molecular Biology
Jamin B. Hein, Hieu T. Nguyen, Dimitriya H. Garvanska, Isha Nasa, Thomas Kruse, Yinnian Feng, Blanca Lopez Mendez, Norman Davey, Arminja N. Kettenbach, Polly M. Fordyce, Jakob Nilsson
Summary: Investigating the specificity of phosphoprotein phosphatases (PPPs) has been challenging, but this study introduces a novel approach, MRBLE:Dephos, to determine phosphatase preferences. Using this method, the authors identified amino acid preferences of residues surrounding the dephosphorylation site for PP1 and PP2A-B55, and discovered key dephosphorylation sites during mitotic exit that are consistent with MRBLE:Dephos results.
MOLECULAR SYSTEMS BIOLOGY
(2023)
Article
Cell Biology
T Kruse, B Blagoev, A Lobner-Olesen, M Wachi, K Sasaki, N Iwai, M Mann, K Gerdes
GENES & DEVELOPMENT
(2006)