Article
Pharmacology & Pharmacy
Casey-Tyler Berezin, Nikolas Bergum, Glenda M. Torres Lopez, Jozsef Vigh
Summary: Opioids are effective analgesics, but their dangerous side effects need to be considered. Research on opioid pharmacokinetics provides crucial information on drug effects. This study found that morphine accumulates in the mouse retina at higher concentrations than in the brain and that reduced P-gp expression may be the cause. P-gp was identified as the main opioid transporter at the blood-retina barrier, and its expression correlated with morphine concentration in the retina.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Environmental Sciences
Yuzhou Gu, Shengjie Hao, Kaiyuan Liu, Mengqin Gao, Bing Lu, Feiyin Sheng, Li Zhang, Yili Xu, Di Wu, Yu Han, Shuying Chen, Wei Zhao, Xiaoming Lou, Xiaofeng Wang, Peng Li, Zhijian Chen, Ke Yao, Qiuli Fu
Summary: This study explored the effects of PM2.5 on the inner blood-retinal barrier, finding that exposure to PM2.5 resulted in retinal inflammation, vascular dilation, and damage to the iBRB.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Review
Pharmacology & Pharmacy
Patrick T. Ronaldson, Thomas P. Davis
Summary: Ischemic stroke is a major cause of morbidity and mortality globally. Despite positive results in preclinical studies, only one compound has advanced to Phase III clinical trial evaluation. This may be due to the neglect of transport mechanisms at the blood-brain barrier and neurovascular unit. This review highlights the role of BBB transporters in drug delivery and their expression in different brain cell types.
Review
Pharmacology & Pharmacy
Valerio Taggi, Mario Riera Romo, Micheline Piquette-Miller, Henriette E. Meyer zu Schwabedissen, Sibylle Neuhoff
Summary: Drug transporters are crucial for maintaining chemical balance and homeostasis in tissues, as well as for the absorption, distribution, and elimination of clinically important drugs. Diseases can alter the expression and function of drug transporters. However, there is limited knowledge on transporter regulation in critical protective barriers, which requires further research.
Article
Pharmacology & Pharmacy
C. Goldeman, M. Andersen, A. Al-Robai, T. Buchholtz, N. Svane, B. Ozgur, B. Holst, E. Shusta, V. J. Hall, L. Saaby, P. Hyttel, B. Brodin
Summary: This study investigated the differentiation of human induced pluripotent stem cells (hiPSCs) into brain capillary endothelial-like cells (BCEC) for potential use in drug delivery research. The results demonstrated that the BCEC monolayers exhibited properties of the blood-brain barrier, including barrier tightness, high dynamic range, and functional transport capabilities.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Michele Galluccio, Lara Console, Lorena Pochini, Mariafrancesca Scalise, Nicola Giangregorio, Cesare Indiveri
Summary: Membrane transport systems play a crucial role in maintaining cell homeostasis and drug interactions. E. coli is a preferred host for expressing human membrane proteins, but their hydrophobicity and structural differences pose challenges. This review summarizes various strategies for expressing human transport systems in bacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Health Care Sciences & Services
Miriam Saiz-Rodriguez, Dolores Ochoa, Pablo Zubiaur, Marcos Navares-Gomez, Manuel Roman, Paola Camargo-Mamani, Sergio Luquero-Bueno, Gonzalo Villapalos-Garcia, Raquel Alcaraz, Gina Mejia-Abril, Estefania Santos-Mazo, Francisco Abad-Santos
Summary: For patients with type 2 diabetes, the drug metformin is commonly prescribed, but the response to this drug varies greatly among individuals. In order to study the impact of transporter variations on metformin pharmacokinetics, a study was conducted on healthy participants without confounding factors. This study is the first of its kind to consider demographic characteristics and all transporters involved in metformin distribution. The pharmacokinetic parameters of metformin were found to be influenced by age, sex, ethnicity, and several transporter polymorphisms.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Review
Pharmacology & Pharmacy
Lenka Hruba, Viswanath Das, Marian Hajduch, Petr Dzubak
Summary: Nucleoside-based drugs, widely used in the treatment of various diseases, have been hindered by drug resistance. This review explores the mechanisms of resistance, including changes in membrane transporters, activating kinases, drug elimination strategies, and DNA damage repair mechanisms. The critical analysis in this review points towards novel therapeutic strategies such as targeted therapies, new nucleoside analogs, and synergistic drug combinations to overcome resistance and improve patient outcomes.
BIOCHEMICAL PHARMACOLOGY
(2023)
Review
Cell Biology
Ilenia Martinelli, Seyed Khosrow Tayebati, Daniele Tomassoni, Giulio Nittari, Proshanta Roy, Francesco Amenta
Summary: This article reviews the development and technical analysis of brain and retinal organoids, and discusses their potential in modeling neurological and retinal diseases, as well as their use as screening tools. Furthermore, potential future directions, such as dedicated biobanks, are proposed.
Article
Pharmacology & Pharmacy
Alexandre Bourdin, Manon Ortoli, Remi Karadayi, Lauriane Przegralek, Florian Sennlaub, Bahram Bodaghi, Xavier Guillonneau, Alexandre Carpentier, Sara Touhami
Summary: Sonication with microbubble-assisted low-intensity pulsed ultrasound is a non-invasive method to transiently open the blood-retina barrier (BRB), increasing retinal diffusion of systemic drugs without noticeable side-effects.
Review
Biochemistry & Molecular Biology
Felix Yemanyi, Kiran Bora, Alexandra K. Blomfield, Zhongxiao Wang, Jing Chen
Summary: The retina is a light-sensing tissue that relays information to the brain for vision. The blood-retinal barrier (BRB) contributes to maintaining homeostasis and protecting the retina from toxins. Disruption of the inner BRB (iBRB) can lead to conditions like retinal edema and vision loss. Understanding the mechanisms of iBRB formation, maintenance, and breakdown is crucial for potential therapeutic targets and drug delivery strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Gokce Gurler, Nevin Belder, Mustafa Caglar Beker, Melike Sever-Bahcekapili, Gokhan Uruk, Ertugrul Kilic, Muge Yemisci
Summary: This study reveals the presence of RFC1 protein in the inner blood-retinal barrier (BRB) and its role as an acute regulator in both normal and ischemic retinas.
FLUIDS AND BARRIERS OF THE CNS
(2023)
Review
Biology
Lara Console, Mariafrancesca Scalise
Summary: Extracellular vesicles, including exosomes, play a crucial role in promoting chemoresistance in cancer cells through various mechanisms.
Article
Biochemistry & Molecular Biology
Anne Cloe Voirin, Morgane Chatard, Anne Briancon-Marjollet, Jean Louis Pepin, Nathalie Perek, Frederic Roche
Summary: Several sleep-related breathing disorders can lead to neurological diseases, such as cognitive impairment, due to repeated hypoxia stresses. However, the consequences of repeated intermittent hypoxia on the blood-brain barrier (BBB) are not well recognized. This study compared two methods of intermittent hypoxia induction and found that both hydralazine and intermittent physical hypoxia progressively altered BBB integrity, with an increase in Na-Fl permeability and a decrease in tight junction protein concentration. The study also observed an adaptation of the BBB after the third cycle of intermittent hypoxia exposure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Craig Russell, Majad Hussain, David Huen, Ayesha S. Rahman, Afzal R. Mohammed
Summary: This study explores the use of DNA microarrays as a high throughput screening tool to identify markers for predicting bioavailability and formulation-driven physiological responses. Significant changes in gene expression were found as a result of formulation exposure, including genes related to drug transport and metabolism.
PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY
(2021)
Article
Developmental Biology
Christiane Albrecht, Larry Chamley, D. Stephen Charnock-Jones, Sally Collins, Hiroshi Fujiwara, Thaddeus Golos, Solene Grayo, Natalie Hannan, Lynda Harris, Kiyotake Ichizuka, Nicholas P. Illsley, Mitsutoshi Iwashita, Sampada Kallol, Abdulla Al-Khan, Gendie Lash, Takeshi Nagamatsu, Akitoshi Nakashima, Kaoru Niimi, Masataka Nomoto, Christopher Redman, Shigeru Saito, Kenji Tanimura, Masatoshi Tomi, Hirokazu Usui, Manu Vatish, Bryce Wolfe, Eiko Yamamoto, Perrie O'Tierney-Ginn
Article
Chemistry, Medicinal
Tomohiro Nishimura, Yuichiro Sano, Yu Takahashi, Saki Noguchi, Yasuo Uchida, Akinori Takagi, Takahiro Tanaka, Satomi Katakura, Emi Nakashima, Masanori Tachikawa, Tetsuo Maruyama, Tetsuya Terasaki, Masatoshi Tomi
JOURNAL OF PHARMACEUTICAL SCIENCES
(2019)
Article
Multidisciplinary Sciences
Mai Inagaki, Tomohiro Nishimura, Takeo Nakanishi, Hiroaki Shimada, Saki Noguchi, Shin-ichi Akanuma, Masanori Tachikawa, Ken-ichi Hosoya, Ikumi Tamai, Emi Nakashima, Masatoshi Tomi
Article
Pharmacology & Pharmacy
Ken Kurosawa, Koji Chiba, Saki Noguchi, Tomohiro Nishimura, Masatoshi Tomi
DRUG METABOLISM AND DISPOSITION
(2020)
Article
Pharmacology & Pharmacy
Saki Noguchi, Moeko Okochi, Hayumi Atsuta, Rika Kimura, Ayaka Fukumoto, Kyoko Takahashi, Tomohiro Nishimura, Masatoshi Tomi
Summary: OAT4 may play a role in the excretion of azilsartan, candesartan, carboxylosartan, and valsartan, as well as olmesartan. Only OAT4-mediated dianion transport is Cl- sensitive.
DRUG METABOLISM AND PHARMACOKINETICS
(2021)
Article
Pharmacology & Pharmacy
Saki Noguchi, Akinori Takagi, Takahiro Tanaka, Yu Takahashi, Xiaole Pan, Yuka Kibayashi, Ryo Mizokami, Tomohiro Nishimura, Masatoshi Tomi
Summary: The study revealed that ENT1 expression was higher than ENT2 in triple-negative breast cancer cell lines, but both transporters had minimal contribution to fluorouracil uptake and showed no correlation with fluorouracil sensitivity. It suggests that neither ENT1 nor ENT2 significantly affects the sensitivity of TNBC cell lines to fluorouracil.
BIOPHARMACEUTICS & DRUG DISPOSITION
(2021)
Article
Biochemistry & Molecular Biology
Xiaole Pan, Saki Noguchi, Misuzu Ando, Tomohiro Nishimura, Masatoshi Tomi
Summary: The study found that overexpression of miR-126 can inhibit the invasive ability of JEG-3 cells by targeting LIN28A, without affecting proliferation and migration. In addition, miR-126-mediated downregulation of LIN28A may contribute to the onset/deterioration of preeclampsia.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Meeting Abstract
Developmental Biology
Masatoshi Tomi, Takehiro Nomura, Saki Noguchi, Tomohiro Nishimura
Meeting Abstract
Pharmacology & Pharmacy
Tomohiro Nishimura, Yu Ishikawa, Saki Noguchi, Masatoshi Tomi
DRUG METABOLISM AND PHARMACOKINETICS
(2020)
Meeting Abstract
Pharmacology & Pharmacy
Saki Noguchi, Moeko Tobita, Hayumi Atsuta, Rika Kimura, Ayaka Fukumoto, Tomohiro Nishimura, Masatoshi Tomi
DRUG METABOLISM AND PHARMACOKINETICS
(2020)
Meeting Abstract
Developmental Biology
Minako Tanabe, Saki Noguchi, Tomohiro Nishimura, Masatoshi Tomi
Meeting Abstract
Developmental Biology
Tomohiro Nishimura, Masaya Takahashi, Hikari Araki, Saki Noguchi, Masatoshi Tomi
Meeting Abstract
Developmental Biology
Saki Noguchi, Kanako Furugori, Tomohiro Nishimura, Emi Nakashima, Masatoshi Tomi
Meeting Abstract
Developmental Biology
Mai Inagaki, Tomohiro Nishimura, Takeo Nakanishi, Hiroaki Shimada, Saki Noguchi, Shin-ichi Akanuma, Masanori Tachikawa, Emi Nakashima, Ken-ichi Hosoya, Ikumi Tamai, Masatoshi Tomi
Article
Chemistry, Medicinal
M. Isawa, R. Tashir, C. Naruse, Y. Yamaguchi, H. Itoh, T. Nishimura, M. Tomi, H. Shimada, H. Saito, M. Mochizuki, E. Nakashima