Article
Parasitology
Huijiao Jiang, Xiaoyi Wang, Lijiao Guo, Xiaowu Tan, Xianwei Gui, Zhenyu Liao, Zhiwei Li, Xueling Chen, Xiangwei Wu
Summary: The study demonstrated that SU11248 effectively inhibited the growth of E. multilocularis metacestodes and reduced neovascular lesions. The expression of pro-angiogenic factors VEGFA, VEGFR2, and p-VEGFR2 was significantly decreased in the metacestode tissues after SU11248 treatment. It also showed some anthelmintic effects in vitro, damaging the cellular structure of the metacestodes. Moreover, SU11248 was less toxic to certain cells and completely inhibited EPC migration.
PARASITES & VECTORS
(2023)
Review
Chemistry, Analytical
Ketki Bagwe, Noah Gould, Kendall R. Johnson, Alexander R. Ivanov
Summary: Tissues and cell populations exhibit high levels of heterogeneity at the cellular level, making it important to analyze smaller sample sizes to capture subtle differences between cells. Capillary electrophoresis coupled with mass spectrometry (CE-MS) is a powerful technique for proteomics and metabolomics analysis at the single-cell level, and this review focuses on its applications and recent advances in sample preparation, separation, MS acquisition, and data analysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Immunology
Pei Zhou, Zhen Zhou, Meiduo Huayu, Lei Wang, Lin Feng, Yang Xiao, Yao Dai, Mingyuan Xin, Feng Tang, Runle Li
Summary: In this study, a multi-epitope vaccine GILE containing dominant epitopes of Echinococcus Multilocularis (E. multilocularis) infection was successfully constructed. The GILE vaccine showed good immunogenicity and antigenicity, providing a theoretical and experimental basis for the prevention and treatment of E. multilocularis infection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Congshan Liu, Jianping Cao, Haobing Zhang, Mark C. Field, Jianhai Yin
Summary: The study suggests that tapeworm-secreted extracellular vesicles (EVs) may promote angiogenesis in Echinococcus infections, revealing central mechanisms of Echinococcus-host interactions.
MICROBES AND INFECTION
(2023)
Article
Biochemical Research Methods
Martha Beatriz Morales-Amparano, Alejandra Valenzuela-Corral, Gabriela Ramos-Clamont Montfort, Luz Vazquez-Moreno, Abraham Escobedo-Moratilla, Guillermo Pastor-Palacios, Cesare Ovando-Vazquez, Luis M. Teran, Jose Angel Huerta-Ocampo
Summary: Pecan pollen is a significant cause of allergic respiratory disease, with 17 IgE binding proteins identified in this study. These findings are crucial for the development of specific diagnosis and treatment for pecan pollen allergy.
JOURNAL OF PROTEOMICS
(2021)
Article
Chemistry, Analytical
Daoyang Chen, Zhichang Yang, Xiaojing Shen, Liangliang Sun
Summary: Top-down proteomics (TDP) is an ideal approach for deciphering the histone code and requires new analytical tools with high-capacity separation and highly sensitive detection of proteoforms. In this study, capillary zone electrophoresis (CZE) -MS/MS was used to identify a large number of histone proteoforms with higher sensitivity compared to traditional methods.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Sille Stepanova, Vaclav Kasicka
Summary: This review provides an overview of recent advances and applications of capillary electrophoresis and microchip capillary electrophoresis methods in proteomics and peptidomics. It covers topics such as sample preparation, hyphenation with mass spectrometry, and multidimensional separations. New developments in both bottom-up and top-down approaches in proteomics are discussed, along with various applications in protein posttranslational modifications, biological and biochemical research, clinical peptidomics and proteomics, and food analysis.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Emmanouil Mavrogeorgis, Harald Mischak, Agnieszka Latosinska, Justyna Siwy, Vera Jankowski, Joachim Jankowski
Summary: In recent years, CE-MS has been increasingly used in clinical research for chronic and age-associated diseases, allowing for comprehensive assessment of small molecular weight proteins and peptides. The study evaluated the analytical performance of CE-MS in terms of reproducibility, variability, and efficiency in peptide detection, showing high correlation among different runs and low variation in signal intensity. These findings support the value of CE-MS for clinical application.
Article
Chemistry, Analytical
Julien Slagboom, Chiel Kaal, Arif Arrahman, Freek J. Vonk, Govert W. Somsen, Juan J. Calvete, Wolfgang Wuster, Jeroen Kool
Summary: Snakebite envenoming is a global issue, and characterizing snake venom can help in developing efficient antivenoms. Various analytical methods such as venomics have been used to analyze the composition and properties of snake venom toxins. The study involves sample preparation, separation, detection, and quantitation techniques.
MICROCHEMICAL JOURNAL
(2022)
Article
Parasitology
Sebastian Miles, Javier Magnone, Joaquin Garcia-Luna, Sylvia Dematteis, Gustavo Mourglia-Ettlin
Summary: This study provides insights into the post-translational modifications (PTMs) of proteins in E. granulosus s.l., including the identification of new PTMs and the enzymes involved. These findings expand our knowledge on PTMs in platyhelminthes.
Article
Biochemistry & Molecular Biology
Marie-Jia Gou, Murat Cem Kose, Jacques Crommen, Cindy Nix, Gael Cobraiville, Jo Caers, Marianne Fillet
Summary: By combining different separation strategies to increase the number of identified proteins in a sample, it can aid in antigen discovery research. Studies have shown that the combination of CZE-DTIMS-QTOF and microfluidic RPLC can provide unique information.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Meletia Kallianta, Eftychia Pappa, Heleni Vastardis, Christos Rahiotis
Summary: Mass Spectrometry (MS) is a highly sensitive and universal analytical technique that has rapidly developed in recent decades. It has various applications in dentistry, including research on dental materials and agents, laboratory analysis of biospecimens, and real-time diagnostics in oral surgery and pathology. MS can provide valuable insights into the clinical behavior, toxicity, antimicrobial properties, and biomarkers of dental materials and agents. It is also effective in detecting biomarkers in saliva and providing a comprehensive understanding of cellular functions through metabolite analysis. Additionally, MS shows promising potential for surgical approaches to oral malignant disorders. Overall, MS has revolutionized dental research and has the potential to advance personalized dentistry.
Review
Biochemistry & Molecular Biology
Ruben Agregan, Noemi Echegaray, Maria Lopez-Pedrouso, Radwan Kharabsheh, Daniel Franco, Jose M. Lorenzo
Summary: Proteomics has made significant advances in the field of medicine and has applications in other scientific areas, such as the study of milk. Milk proteomics can help characterize milk samples and products in detail, with significant applications in industry for quality control and improvement.
Article
Parasitology
Grazia Biosa, Piero Bonelli, Salvatore Pisanu, Stefania Ghisaura, Cinzia Santucciu, Angela Peruzzu, Giovanni Garippa, Sergio Uzzau, Giovanna Masala, Daniela Pagnozzi
Summary: The proteome of hydatid cyst fluids of Echinococcus granulosus and other ovine parasites were analyzed, and potential diagnostic biomarkers for cystic echinococcosis (CE) were identified, which can predict CE in sheep and differentiate between different types of parasites.
Article
Biochemical Research Methods
Kendall R. Johnson, Michal Gregus, Alexander R. Ivanov
Summary: In this study, the combination of high-field asymmetric waveform ion mobility spectrometry (FAIMS) with ultrasensitive capillary electrophoresis hyphenated with tandem mass spectrometry (CE-MS/MS) was used for deep proteome coverage of low nanogram amounts of digested cell lysates. The optimized CE-FAIMS-MS/MS method showed significant improvements in protein and peptide identification, as well as detection of multiphosphorylated peptides.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Parasitology
Zhe Cheng, Shan Zhu, Liang Wang, Fan Liu, Huimin Tian, Theerakamol Pengsakul, Yanhai Wang
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2015)
Article
Parasitology
Zhe Cheng, Fan Liu, Mengya Dai, Jianjian Wu, Xiu Li, Xinrui Guo, Huimin Tian, Zhijie Heng, Ying Lu, Xiaoli Chai, Yanhai Wang
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2017)
Article
Infectious Diseases
Zhe Cheng, Fan Liu, Xiu Li, Mengya Dai, Jianjian Wu, Xinrui Guo, Huimin Tian, Zhijie Heng, Ying Lu, Xiaoli Chai, Yanhai Wang
PLOS NEGLECTED TROPICAL DISEASES
(2017)
Article
Cell Biology
Qinxi Li, Shuyong Lin, Xuan Wang, Guili Lian, Zailian Lu, Huiling Guo, Ka Ruan, Yanhai Wang, Zhiyun Ye, Jiahuai Han, Sheng-Cai Lin
NATURE CELL BIOLOGY
(2009)
Article
Multidisciplinary Sciences
Zhe Cheng, Fan Liu, Shan Zhu, Huimin Tian, Liang Wang, Yanhai Wang
Article
Toxicology
Zhe Cheng, Huimin Tian, Hongran Chu, Jianjian Wu, Yingying Li, Yanhai Wang
TOXICOLOGY LETTERS
(2014)
Article
Cell Biology
Sangun Lee, Yanhai Wang, Sung Ouk Kim, Jiahuai Han
Article
Infectious Diseases
Zhe Cheng, Fan Liu, Huimin Tian, Zhijian Xu, Xiaoli Chai, Damin Luo, Yanhai Wang
PLOS NEGLECTED TROPICAL DISEASES
(2019)
Article
Microbiology
Zhe Cheng, Zhijian Xu, Huimin Tian, Fan Liu, Xiu Li, Damin Luo, Yanhai Wang
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2020)
Article
Microbiology
Chonglv Feng, Zhe Cheng, Zhijian Xu, Ye Tian, Huimin Tian, Fan Liu, Damin Luo, Yanhai Wang
Summary: This study describes the cell cycle regulation mechanism of germinative cells in the tapeworm Echinococcus multilocularis, which is influenced by the interaction between the parasite and the host. Host EGF regulates the G1/S phase of the cell cycle through the EGFR-ERK-EmCyclinD pathway.
FRONTIERS IN MICROBIOLOGY
(2022)
