Article
Endocrinology & Metabolism
Young Gun Park, Ji-Yeon Lee, Chongtae Kim, Young-Hoon Park
Summary: This study found that experimental diabetic retinopathy causes gradual activation of retinal microglia and migration of macrophages, with the activation changing typically with the progression of the disease.
JOURNAL OF DIABETES RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Michal Wszola, Marta Klak, Anna Kosowska, Grzegorz Tymicki, Andrzej Berman, Anna Adamiok-Ostrowska, Joanna Olkowska-Truchanowicz, Izabela Uhrynowska-Tyszkiewicz, Artur Kaminski
Summary: The study investigated the use of streptozotocin (STZ) to induce diabetes in mice, leading to over 90% destruction of beta-cell mass. However, STZ doses below 300 mg/kg did not show significant changes in islet area and insulin-positive areas in mice.
Article
Nutrition & Dietetics
Junhui Shen, Li Zhang, Yuanqi Wang, Zhiqing Chen, Jian Ma, Xiaoyun Fang, Undurti N. Das, Ke Yao
Summary: This study investigates the effects of ALA and LA on the metabolism, gut microbiota, and inflammatory and retinal indices in T1DM animals. The results show that ALA and LA have significant benefits in reversing the changes seen in T1DM, suggesting their importance in diabetes mellitus.
FRONTIERS IN NUTRITION
(2022)
Article
Medicine, Research & Experimental
Mohamed Sadek Abdel-Bakky, Abdulmajeed Alqasoumi, Waleed Mohammad Altowayan, Elham Amin, Mostafa Assem Darwish
Summary: The study investigated the roles of ADAM10 and ADAM17 in T1DM, and the protective effects of SIM in STZ-induced T1DM. The results showed that SIM could reduce pancreatic inflammation and cell apoptosis, and decrease the expression of key proteins, alleviating the pathological damage of T1DM.
Article
Pharmacology & Pharmacy
Jieming Fu, Moe H. Aung, Megan C. Prunty, Adam M. Hanif, Lauren M. Hutson, Jeffrey H. Boatright, Machelle T. Pardue
Summary: The study demonstrated that early treatment with TUDCA preserved visual and retinal function in a mouse model of early diabetic retinopathy, with better outcomes seen with early treatment compared to late treatment.
Article
Cell Biology
Luiz A. D. Queiroz, Josiane B. Assis, Joao P. T. Guimaraes, Emanuella S. A. Sousa, Analia C. Milhomem, Karen K. S. Sunahara, Anderson Sa-Nunes, Joilson O. Martins
Summary: Alloxan (ALX) and streptozotocin (STZ) are commonly used to induce type 1 diabetes in animal models, but they lead to different immune responses. STZ-induced diabetes showed significant immune cell parameter changes in vivo and in vitro early in the disease, while ALX-induced diabetes resulted in alterations predominantly in the chronic phase, leading to increased mortality rates.
MEDIATORS OF INFLAMMATION
(2021)
Article
Biochemistry & Molecular Biology
Camila Moreno Rosa, Dijon Henrique Salome Campos, David Rafael Abreu Reyes, Felipe Cesar Damatto, Lucas Yamada Kurosaki, Luana Urbano Pagan, Mariana Janini Gomes, Camila Renata Correa, Ana Angelica Henrique Fernandes, Marina Politi Okoshi, Katashi Okoshi
Summary: Clinical trials have shown that SGLT2 inhibitors improve clinical outcomes in patients with diabetes mellitus. This study aimed to analyze the effects of the SGLT2 inhibitor dapagliflozin on cardiac remodeling in a rat model of Type 1 diabetes mellitus. The results demonstrated that dapagliflozin improved blood pressure, glycemia, and cardiac function, and attenuated cardiac changes in the experimental rat model.
Article
Neurosciences
Aiwen Xu, Michael Beyeler
Summary: This study used a biophysical model to simulate the retina and investigated the impact of neuroanatomical changes on cellular responses during retinal degeneration. The findings revealed that retinal cell activity increased and electrical stimulation thresholds also increased during retinal degeneration, and testable predictions about the underlying neuroanatomical mechanisms were proposed.
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Multidisciplinary Sciences
M. Parravano, L. Ziccardi, E. Borrelli, E. Costanzo, S. Frontoni, F. Picconi, V. Parisi, R. Sacconi, A. Di Renzo, M. Varano, G. Querques
Summary: The study revealed differences in outer retina function and CC perfusion characteristics between NPDR and NoDR T1D patients, with significantly greater flow deficit average area in NPDR eyes compared to NoDR eyes, while mfERG-RADs were similar in both groups. In addition, a positive correlation between outer retina dysfunction and CC impairment was observed in NPDR eyes, while a negative correlation between photoreceptor reflectivity and CC abnormalities was seen in NoDR eyes.
SCIENTIFIC REPORTS
(2021)
Article
Neurosciences
Tong Zhu, Yingao Li, Lilin Zhu, Jinyuan Xu, Zijun Feng, Hao Chen, Si Shi, Caiying Liu, Qingjian Ou, Furong Gao, Jieping Zhang, Caixia Jin, Jingying Xu, Jiao Li, Jingfa Zhang, Yanlong Bi, Guo-tong Xu, Juan Wang, Haibin Tian, Lixia Lu
Summary: This study identified GMFB as a sensitive and functional gliosis marker for diabetic retinal degeneration. Knockout of Gmfb improved visual function, attenuated gliosis, and reduced apoptosis in diabetic retinas. RNA sequencing analysis revealed the involvement of TGF-beta 3 in the protective effect of Gmfb knockout. The study provides a novel therapeutic strategy for treating retinal degeneration in patients with diabetic retinopathy.
Article
Biochemistry & Molecular Biology
Fabiola Marino, Nadia Salerno, Mariangela Scalise, Luca Salerno, Annalaura Torella, Claudia Molinaro, Antonio Chiefalo, Andrea Filardo, Chiara Siracusa, Giuseppe Panuccio, Carlo Ferravante, Giorgio Giurato, Francesca Rizzo, Michele Torella, Maria Donniacuo, Antonella De Angelis, Giuseppe Viglietto, Konrad Urbanek, Alessandro Weisz, Daniele Torella, Eleonora Cianflone
Summary: Diabetic cardiomyopathy (DCM) is a major cause of cardiovascular complications in diabetes mellitus (DM). Two different types of diabetes, type 1 and type 2, exhibit different effects on cardiac structure, function and gene expression. Animal models induced by different glucose regulators have been used to compare and study these effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xianrong Zhou, Guan-sheng Shang, Qian Tan, Qin He, Xiaoyu Tan, Kun-Young Park, Xin Zhao
Summary: L. fermentum TKSN041 shows potential preventive and therapeutic effects on type 2 diabetes in rats, reducing water intake, controlling blood glucose levels, decreasing harmful substances, and protecting organ functions.
Article
Ophthalmology
Vivian Schreur, Heijan Ng, Giels Nijpels, Einar Stefansson, Cees J. Tack, B. Jeroen Klevering, Eiko K. de Jong, Carel B. Hoyng, Jan E. E. Keunen, Amber A. van der Heijden
Summary: This study validated a model for predicting diabetic retinopathy in persons with type 1 diabetes, demonstrating that a personalised screening program could reduce screening frequency and had good discriminatory ability.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2021)
Article
Immunology
Zachary J. J. Morse, Rachel L. L. Simister, Sean A. A. Crowe, Marc S. S. Horwitz, Lisa C. C. Osborne
Summary: Autoimmune disorders are complex diseases whose etiology is unclear. Evidence suggests that genetic susceptibility and environmental factors play a critical role. In type 1 diabetes (T1D), enterovirus infection and disruption of the intestinal microbiota have been independently associated with T1D onset. However, the interaction between viral infection and the intestinal microbiota remains unknown.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Marlon R. Schneider, Silja Zettler, Birgit Rathkolb, Maik Dahlhoff
Summary: TXNIP deficiency promotes beta cell survival, while overexpression of TXNIP can lead to increased blood glucose levels, impaired glucose tolerance, and worsen the severity of type 1 diabetes.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Deokho Lee, Yohei Tomita, Lizhu Yang, Kazuno Negishi, Toshihide Kurihara
Summary: This article reviews the pathophysiology of ocular ischemic syndrome, including experimental models and clinical cases. It also discusses current treatment options and promising preclinical treatments with recent advancements.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Tomoki Maruyama, Erisa Yotsukura, Hidemasa Torii, Kiwako Mori, Mikako Inokuchi, Mitsuaki Tokumura, Debabrata Hazra, Mamoru Ogawa, Akiko Hanyuda, Kazuo Tsubota, Toshihide Kurihara, Kazuno Negishi
Summary: The prevalence of myopia is high among Japanese schoolchildren, with almost half of preschoolers being myopic. The time spent using digital devices is positively associated with lens thickness, while reading time is negatively associated with lens thickness, spherical equivalent, axial length, and vitreous chamber depth.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Deokho Lee, Yohei Tomita, Yukihiro Miwa, Ari Shinojima, Norimitsu Ban, Shintaro Yamaguchi, Ken Nishioka, Kazuno Negishi, Jun Yoshino, Toshihide Kurihara
Summary: The study suggests that NMN administration offers protection against retinal ischemia/reperfusion injury by suppressing inflammation, protecting retinal neuronal cells, and activating the antioxidant pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Shin-ichi Ikeda, Toshihide Kurihara, Xiaoyan Jiang, Yukihiro Miwa, Deokho Lee, Naho Serizawa, Heonuk Jeong, Kiwako Mori, Yusaku Katada, Hiromitsu Kunimi, Nobuhiro Ozawa, Chiho Shoda, Mari Ibuki, Kazuno Negishi, Hidemasa Torii, Kazuo Tsubota
Summary: Axial length is the primary determinant of eye size and is elongated in myopia. This study reveals that endoplasmic reticulum (ER) stress in the sclera and the PERK/ATF6 pathway are involved in the axial elongation during myopia development. The researchers also demonstrate that 4-PBA eye drops show promise as a drug for myopia suppression/treatment.
NATURE COMMUNICATIONS
(2022)
Article
Medicine, General & Internal
Hidemasa Torii, Kiwako Mori, Takashi Okano, Shinichiro Kondo, Hao-Yung Yang, Erisa Yotsukura, Akiko Hanyuda, Mamoru Ogawa, Kazuno Negishi, Toshihide Kurihara, Kazuo Tsubota
Summary: This study investigated the safety and efficacy of eyeglasses emitting violet light for myopia suppression. The results showed that VL-emitting eyeglass frames had short-term safety and effectiveness against myopia progression, with significant changes observed in axial elongation, choroidal thickness, and cycloplegic refractions in a subgroup of 8- to 10-year-old children.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Deokho Lee, Yohei Tomita, Yukihiro Miwa, Heonuk Jeong, Ari Shinojima, Norimitsu Ban, Shintaro Yamaguchi, Ken Nishioka, Kazuno Negishi, Jun Yoshino, Toshihide Kurihara
Summary: This study demonstrated for the first time the protective effect of NMN in cardiovascular disease-induced retinal ischemia in a mouse model, suppressing retinal dysfunction and pathological gliosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Ophthalmology
Heonuk Jeong, Toshihide Kurihara, Xiaoyan Jiang, Shinichiro Kondo, Yusuke Ueno, Yuki Hayashi, Deokho Lee, Shin-Ichi Ikeda, Kiwako Mori, Hidemasa Torii, Kazuno Negishi, Kazuo Tsubota
Summary: The prevalence of myopia has been increasing steadily for decades, causing significant medical and economic issues in society. Exposure to violet light (VL) has been proposed as an effective treatment for preventing and suppressing myopia development. This study investigated the effects of VL on myopia progression, and found that different transmittances of VL can lessen the myopic shift and axial length difference. VL exposure also suppressed choroidal thinning observed in myopia. The findings suggest that managing myopia progression can be achieved through VL transmittance.
EXPERIMENTAL EYE RESEARCH
(2023)
Review
Ophthalmology
Deokho Lee, Yohei Tomita, Ari Shinojima, Norimitsu Ban, Shintaro Yamaguchi, Ken Nishioka, Kazuno Negishi, Jun Yoshino, Toshihide Kurihara
Summary: The burden of ocular diseases is increasing worldwide. Nicotinamide mononucleotide (NMN), a bioactive molecule, has potential therapeutic roles in ocular diseases by modulating inflammation, oxidative stress, and metabolic dysregulation. NMN treatment may be beneficial for the prevention and protection from various ocular diseases.
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yifan Liang, Shin-ichi Ikeda, Junhan Chen, Yan Zhang, Kazuno Negishi, Kazuo Tsubota, Toshihide Kurihara
Summary: Myopia is a leading cause of vision impairment, and an effective intervention is needed. This study examined the effects of different forms of lactoferrin (LF) on myopia in mice and found that digested LF and holo-LF were more effective in suppressing myopia compared to native LF. The results also revealed that mice given digested LF or holo-LF had a less elongated axial length and thinner choroid, as well as lower levels of certain cytokines and growth factors associated with myopia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Satoshi Imanishi, Yohei Tomita, Kazuno Negishi, Kazuo Tsubota, Toshihide Kurihara
Summary: Disorders in the development and regulation of blood vessels are involved in various ocular disorders. Understanding the developing choroidal circulation system expands our knowledge of ocular development and supports our understanding of ocular disorders. This review examines studies on regulating the developing choroidal circulation system and discusses its relevance to human diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Ophthalmology
Ziyan Ma, Heonuk Jeong, Yajing Yang, Xiaoyan Jiang, Shin-ichi Ikeda, Kazuno Negishi, Toshihide Kurihara, Kazuo Tsubota
Summary: This study aimed to investigate the contralateral effects of monocular experimental myopia and recovery, which could not be explained by local mechanisms. The results showed that complete recovery of the eyes that wore -30 D lenses was observed after 1 week of lens removal. Both eyes in the PLC and NLC groups exhibited a myopic shift and choroidal thinning after lens removal. The eye wearing the -30 D lens had a significant increase in axial length after 3 weeks of lens wear, while the fellow eyes in the PLC and NLC groups continued to increase in axial length and the binocular axial lengths gradually converged.
OPHTHALMIC AND PHYSIOLOGICAL OPTICS
(2023)
Article
Biochemistry & Molecular Biology
Sayoko Kuroha, Yusaku Katada, Yosuke Isobe, Haruki Uchino, Kyosuke Shishikura, Takashi Nirasawa, Kazuo Tsubota, Kazuno Negishi, Toshihide Kurihara, Makoto Arita
Summary: Docosahexaenoic acid (DHA) and ultra-long-chain polyunsaturated fatty acids (ULC-PUFAs) are enriched in retinal photoreceptor membrane phospholipids. The enzyme ACSL6 plays a crucial role in the synthesis and enrichment of these lipids. Genetic deletion of ACSL6 leads to depletion of di-DHA and ULC-PUFA-containing phospholipids, resulting in visual impairment and progressive decrease of retinal thickness.
Article
Medicine, Research & Experimental
Yusaku Katada, Hiromitsu Kunimi, Naho Serizawa, Deokho Lee, Kenta Kobayashi, Kazuno Negishi, Hideyuki Okano, Kenji F. Tanaka, Kazuo Tsubota, Toshihide Kurihara
Summary: By using the improved KENGE-tet system, researchers successfully expressed the channelrhodopsin gene in RGCs and amacrine cells in a murine model, leading to an enhanced visual restorative effect.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2023)
Article
Ophthalmology
Longdan Kang, Shin-ichi Ikeda, Yajing Yang, Heonuk Jeong, Junhan Chen, Yan Zhang, Kazuno Negishi, Kazuo Tsubota, Toshihide Kurihara
Summary: Recent studies have shown a strong correlation between ER stress and myopia, with eyedrops containing the ER stress inducer Tm inducing myopic changes in mice. The study aimed to create a new myopia model using Tm eyedrops and explore the mechanism of ER stress-mediated myopia development. Tm eyedrops were found to induce myopic changes, decrease scleral collagen expression, and upregulate the expression of matrix metalloproteinase-2, leading to collagen degradation in the sclera.
Article
Multidisciplinary Sciences
Yusaku Katada, Kazuho Yoshida, Naho Serizawa, Deokho Lee, Kenta Kobayashi, Kazuno Negishi, Hideyuki Okano, Hideki Kandori, Kazuo Tsubota, Toshihide Kurihara
Summary: This study generated GHCR by incorporating human rhodopsin into Gloeobacter rhodopsin, which has important implications for the treatment of retinal disorders.