Review
Endocrinology & Metabolism
Ila Tewari Jasra, Nerea Cuesta-Gomez, Kevin Verhoeff, Braulio A. Marfil-Garza, Nidheesh Dadheech, A. M. James Shapiro
Summary: This review summarizes the roles and mechanisms of mitochondria in somatic cell reprogramming to iPSCs and the metabolic shift associated with directed differentiation into pancreatic beta-like cells.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jiangtao Ren, Xuhua Zhang, Zhenhui Zhang, Jiafeng Pan, Zhexue Hao, Jin Li, Jun Liu
Summary: The complex and costly manufacturing process of CAR-T cell therapy has hindered its widespread adoption. Induced pluripotent stem cells (iPSCs) have potential as an alternative cellular immunotherapy due to their unlimited self-renewal potential and ability to differentiate into functional immune cells. Careful selection of the original cell for iPSC seed preparation is crucial due to the presence of epigenetic imprint. Our research shows that lymphocyte-origin cells are superior to embryonic stem cells in differentiating into functional immune cells. Blocking Fas-FasL induced apoptosis in T cells significantly improves iPSC generation without affecting their differentiation potential.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Genetics & Heredity
Huajin Li, Wenting Xu, Sijia Xiang, Leiting Tao, Wen Fu, Jinhui Liu, Wenbin Liu, Yamei Xiao, Liangyue Peng
Summary: Pluripotency is a transient state in early embryos regulated by a network of pluripotency-related genes. This study identified four pluripotent genes in zebrafish and crucian carp and verified that three of them can be used as markers for pluripotency in induced pluripotent stem cells in fish.
FRONTIERS IN GENETICS
(2022)
Article
Cell & Tissue Engineering
Kazuki Morita, Akihiro Nakamura, Masakazu Machida, Tomoyuki Kawasaki, Rina Nakanishi, Justin Ichida, Takanori Iwata, Akihiro Umezawa, Hidenori Akutsu
Summary: Chemical inhibition of the NOTCH signaling pathway and DOT1L promotes the generation of hiPSCs from keratinocytes and significantly increases iPSC colony generation from human fibroblast cells via mRNA reprogramming. The user-friendly reprogramming approach using a single-cell sorting system under efficient conditions opens up possibilities for industrial applications in disease modeling and drug screening.
REGENERATIVE THERAPY
(2022)
Article
Computer Science, Interdisciplinary Applications
Slo-Li Chu, Kazuhiro Sudo, Hideo Yokota, Kuniya Abe, Yukio Nakamura, Ming -Dar Tsai
Summary: This study proposes a method using several deep learning models to efficiently select the reprogramming cells possibly forming hiPSCs and to predict the shapes of growing hiPSC colonies, with the aim of improving the efficiency when establishing a new hiPSC line culture.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Masaaki Ishida, Sunao Sugita, Kenichi Makabe, Shota Fujii, Yoko Futatsugi, Hiroyuki Kamao, Suguru Yamasaki, Noriko Sakai, Akiko Maeda, Michiko Mandai, Masayo Takahashi
Summary: Research has shown that the addition of Y-27632 in vitro can suppress apoptosis, promote cell adhesion and proliferation, and increase the survival rate of transplants. In vivo experiments with human iPS-RPE cell transplantation into monkey eyes showed no obvious retinal toxicity caused by Y-27632.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Shogo Hashimoto, Narihito Nagoshi, Munehisa Shinozaki, Katsuyuki Nakanishi, Yu Suematsu, Takahiro Shibata, Momotaro Kawai, Takahiro Kitagawa, Kentaro Ago, Yasuhiro Kamata, Kaori Yasutake, Ikuko Koya, Yoshinari Ando, Aki Minoda, Tomoko Shindo, Shinsuke Shibata, Morio Matsumoto, Masaya Nakamura, Hideyuki Okano
Summary: In this study, the therapeutic effect of environmental modulation on severe chronic spinal cord injury (SCI) was explored. The use of a clinically relevant hepatocyte growth factor (HGF)-releasing scaffold and human iPS cell-derived neural stem/progenitor cell (hNS/PCs) transplantation led to functional recovery. This study provides evidence for the feasibility of regenerative medicine strategies using clinically relevant materials in the treatment of severe chronic SCI.
Article
Chemistry, Multidisciplinary
Yanan Li, Menghuan Li, Li Liu, Chencheng Xue, Yang Fei, Xuan Wang, Yuchen Zhang, Kaiyong Cai, Yanli Zhao, Zhong Luo
Summary: This study reports a combinational strategy based on clinically tested constituents to selectively induce ferroptosis in metabolically reprogrammed tumor cells, providing a generally applicable therapy.
Article
Biochemistry & Molecular Biology
Haruka Kuriyama, Satoshi Fukushima, Toshihiro Kimura, Hisashi Kanemaru, Azusa Miyashita, Etsuko Okada, Yosuke Kubo, Satoshi Nakahara, Aki Tokuzumi, Yuki Nishimura, Ikko Kajihara, Katsunari Makino, Jun Aoi, Shinichi Masuguchi, Hirotake Tsukamoto, Takashi Inozume, Rong Zhang, Tetsuya Nakatsura, Yasushi Uemura, Satoru Senju, Hironobu Ihn
Summary: By introducing the 4-1BBL gene into iPS-ML, we were able to increase the expression of CD86, promote the upregulation of various cytokines, and enhance the number of antigen-specific CD8(+) T cells. Peritoneal injections of iPS-ML-41BBL not only inhibited tumor growth but also prolonged the survival of mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Kota Suzuki, Shigeru Miyagawa, Li Liu, Takuji Kawamura, Junjun Li, Xiang Qu, Akima Harada, Koichi Toda, Daisuke Yoshioka, Satoshi Kainuma, Ai Kawamura, Yoshiki Sawa
Summary: The study demonstrates that transplantation of aligned cardiac tissue derived from iPS cells improves cardiac function with angiogenesis and antifibrotic effects in a porcine cardiomyopathy model. In both in vitro and in vivo studies, the aligned cardiac tissue containing high purity cardiomyocytes showed homogeneous contraction and excellent mechanical properties, leading to significant improvement in left ventricular ejection fraction compared to the control group. Limited engraftment of the transplanted tissue was observed, but the therapeutic effects were still prominent.
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2021)
Article
Multidisciplinary Sciences
Koki Yoshida, Osamu Uehara, Yoshihito Kurashige, Durga Paudel, Aya Onishi, Puja Neopane, Daichi Hiraki, Tetsuro Morikawa, Fumiya Harada, Rie Takai, Jun Sato, Masato Saitoh, Yoshihiro Abiko
Summary: The combination of a DNA demethylating agent and a histone deacetylase inhibitor can enhance the dedifferentiation of Malassez epithelial cell rests into stem-like cells, with the ability to differentiate into mesenchymal cells. This suggests a potential use for epigenetic agents in promoting periodontal tissue regeneration.
SCIENTIFIC REPORTS
(2021)
Article
Developmental Biology
Prim B. Singh, Assem Zhakupova
Summary: 'Age reprogramming' is a process that rejuvenates the molecular and cellular pathways of a cell without going through an embryonic stage. It helps to understand how to reverse aging while retaining cellular identity and function, which is beneficial for regenerative medicine.
Article
Surgery
Anil Kumar Mavi, Sonal Gaur, Gauri Gaur, Pragya Babita, Pragya Jindal, Neelesh Kumar, Umesh Kumar
Summary: CAR-T cell therapy is a major breakthrough in cancer treatment, but it faces various challenges in solid tumors and hematological malignancies. Severe toxicities, poor anti-tumor effectiveness, antigen escape, restricted trafficking, and limited tumor penetration are barriers to successful CAR-T cell treatment. Additionally, the interactions between CAR-T cells and the host and tumor microenvironment significantly impact their activity. Therefore, innovative methodologies are needed to engineer more potent CAR-T cells with greater anti-tumor activity and less toxicity.
ASIAN JOURNAL OF SURGERY
(2023)
Article
Chemistry, Multidisciplinary
Yunqi Pan, Meiyu Shao, Pan Li, Chen Xu, Jingjun Nie, Kai Zhang, Sen Wu, Dandan Sui, Fu-Jian Xu
Summary: This study proposes a gene therapy strategy for the treatment of diabetes by generating new beta cells from autologous cells. The strategy involves delivering therapeutic genes to the liver using functionalized polymers and inducing the reprogramming of liver cells into beta-like cells. This approach helps alleviate diabetes and its complications by reestablishing endogenous insulin expression.
JOURNAL OF CONTROLLED RELEASE
(2022)
Review
Biochemistry & Molecular Biology
Chenchen Hu, Yuxin Xuan, Xiyang Zhang, Yang Liu, Shuya Yang, Kun Yang
Summary: Energy metabolism is crucial for immune response and studying immune cell metabolism provides valuable insights into immune cell function and disease progression, opening up new possibilities for targeted treatment of inflammatory diseases.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Koichi Oshima, Norikazu Saiki, Michihiro Tanaka, Hiromi Imamura, Akira Niwa, Ayako Tanimura, Ayako Nagahashi, Akiyoshi Hirayama, Keisuke Okita, Akitsu Hotta, Shuichi Kitayama, Mitsujiro Osawa, Shin Kaneko, Akira Watanabe, Isao Asaka, Wataru Fujibuchi, Kohsuke Imai, Hiromasa Yabe, Yoshiro Kamachi, Junichi Hara, Seiji Kojima, Masaru Tomita, Tomoyoshi Soga, Takafumi Noma, Shigeaki Nonoyama, Tatsutoshi Nakahata, Megumu K. Saito
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2018)
Article
Multidisciplinary Sciences
Takashi Ikeda, Takafusa Hikichi, Hisashi Miura, Hirofumi Shibata, Kanae Mitsunaga, Yosuke Yamada, Knut Woltjen, Kei Miyamoto, Ichiro Hiratani, Yasuhiro Yamada, Akitsu Hotta, Takuya Yamamoto, Keisuke Okita, Shinji Masui
NATURE COMMUNICATIONS
(2018)
Article
Cell & Tissue Engineering
Leqian Yu, Junjun Li, Jiayin Hong, Yasuhiro Takashima, Nanae Fujimoto, Minako Nakajima, Akihisa Yamamoto, Xiaofeng Dong, Yujiao Dang, Yu Hou, Wei Yang, Itsunari Minami, Keisuke Okita, Motomu Tanaka, Chunxiong Luo, Fuchou Tang, Yong Chen, Chao Tang, Hidetoshi Kotera, Li Liu
Review
Physiology
Peter Karagiannis, Kazutoshi Takahashi, Megumu Saito, Yoshinori Yoshida, Keisuke Okita, Akira Watanabe, Haruhisa Inoue, Jun K. Yamashita, Masaya Todani, Masato Nakagawa, Mitsujiro Osawa, Yoshimi Yashiro, Shinya Yamanaka, Kenji Osafune
PHYSIOLOGICAL REVIEWS
(2019)
Article
Cell & Tissue Engineering
Huaigeng Xu, Bo Wang, Miyuki Ono, Akihiro Kagita, Kaho Fujii, Noriko Sasakawa, Tatsuki Ueda, Peter Gee, Misato Nishikawa, Masaki Nomura, Fumiyo Kitaoka, Tomoko Takahashi, Keisuke Okita, Yoshinori Yoshida, Shin Kaneko, Akitsu Hotta
Article
Cell & Tissue Engineering
Masaki Yagi, Mio Kabata, Tomoyo Ukai, Sho Ohta, Akito Tanaka, Yui Shimada, Michihiko Sugimoto, Kimi Araki, Keisuke Okita, Knut Woltjen, Konrad Hochedlinger, Takuya Yamamoto, Yasuhiro Yamada
Article
Cell & Tissue Engineering
Yutaka Arai, Miho Takami, Yuri An, Mami Matsuo-Takasaki, Yasuko Hemmi, Tamami Wakabayashi, Jun Inoue, Michiya Noguchi, Yukio Nakamura, Keisuke Sugimoto, Tsukasa Takemura, Keisuke Okita, Kenji Osafune, Minoru Takasato, Tadayoshi Hayata, Yohei Hayashi
STEM CELL RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Tatsuya Shimizu, Shin-Ichi Mae, Toshikazu Araoka, Keisuke Okita, Akitsu Hotta, Kunihiro Yamagata, Kenji Osafune
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Takayuki Hamabata, Katsutsugu Umeda, Kagehiro Kouzuki, Takayuki Tanaka, Tomoo Daifu, Seishiro Nodomi, Satoshi Saida, Itaru Kato, Shiro Baba, Hidefumi Hiramatsu, Mitsujiro Osawa, Akira Niwa, Megumu K. Saito, Yasuhiko Kamikubo, Souichi Adachi, Yoshiko Hashii, Akira Shimada, Hiroyoshi Watanabe, Kenji Osafune, Keisuke Okita, Tatsutoshi Nakahata, Kenichiro Watanabe, Junko Takita, Toshio Heike
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Yuta Yamamura, Kengo Furuichi, Yasuhiro Murakawa, Shigeki Hirabayashi, Masahito Yoshihara, Keisuke Sako, Shinji Kitajima, Tadashi Toyama, Yasunori Iwata, Norihiko Sakai, Kazuyoshi Hosomichi, Philip M. Murphy, Atsushi Tajima, Keisuke Okita, Kenji Osafune, Shuichi Kaneko, Takashi Wada
Summary: This study analyzed gene expression in iPSCs from patients with RCS carrying PAX2 mutations, identifying differentially activated candidate promoters and enhancers, and prioritized 17 candidate genes, with PBX1, POSTN, and ITGA9 ranked as the top three. The identification of these genes may provide important information for clarifying the pathogenesis of RCS, human kidney development, and kidney regeneration.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Shihori Yokobayashi, Yukihiro Yabuta, Masato Nakagawa, Keisuke Okita, Bo Hu, Yusuke Murase, Tomonori Nakamura, Guillaume Bourque, Jacek Majewski, Takuya Yamamoto, Mitinori Saitou
Summary: The research demonstrates significant epigenomic heterogeneity in hiPSCs, with different germline potential correlating with X chromosome inactivation. This heterogeneity is primarily determined by genomic properties.
Article
Cell & Tissue Engineering
Yoshinori Endo, Ken-ichiro Kamei, Kouichi Hasegawa, Keisuke Okita, Hideyuki Ito, Shiho Terada, Miho Inoue-Murayama
Summary: This study generated iPSCs from the endangered Grevy's zebra and described their molecular characteristics. The results suggest evolutionary conservation between these iPSCs and those from humans and mice, to some extent.
STEM CELLS AND DEVELOPMENT
(2022)
Article
Reproductive Biology
Shihori Yokobayashi, Keisuke Okita, Masato Nakagawa, Tomonori Nakamura, Yukihiro Yabuta, Takuya Yamamoto, Mitinori Saitou
BIOLOGY OF REPRODUCTION
(2017)
Article
Biochemistry & Molecular Biology
Elena Yukie Yoshitoshi-Uebayashi, Taro Toyoda, Katsutaro Yasuda, Maki Kotaka, Keiko Nomoto, Keisuke Okita, Kentaro Yasuchika, Shinya Okamoto, Noriyuki Takubo, Toshiya Nishikubo, Tomoyoshi Soga, Shinji Uemoto, Kenji Osafune
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2017)
Article
Cell Biology
Masamitsu Sone, Nobuhiro Morone, Tomonori Nakamura, Akito Tanaka, Keisuke Okita, Knut Woltjen, Masato Nakagawa, John E. Heuser, Yasuhiro Yamada, Shinya Yamanaka, Takuya Yamamoto
