期刊
EXPERIMENTAL AND MOLECULAR MEDICINE
卷 44, 期 2, 页码 130-137出版社
KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY
DOI: 10.3858/emm.2012.44.2.008
关键词
calcium; chemotaxis; neutrophils; peptide library; peptides; receptors, formyl peptide
资金
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2010-0020940]
- Korean government (MEST) [2009 0093198]
- Sungkyunkwan University
- National Research Foundation of Korea [2010-0020940, 2009-0093198] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Neutrophils play a key role in innate immunity, and the identification of new stimuli that stimulate neutrophil activity is a very important issue. In this study, we identified three novel peptides by screening a synthetic hexapeptide combinatorial library. The identified peptides GMMWAI, MMHWAM, and MMHWFM caused an increase in intracellular Ca2+ in a concentration-dependent manner via phospholipase C activity in human neutrophils. The three peptides acted specifically on neutrophils and monocytes and not on other non-leukocytic cells. As a physiological characteristic of the peptides, we observed that the three peptides induced chemotactic migration of neutrophils as well as stimulated superoxide anion production. Studying receptor specificity, we observed that two of the peptides (GMMWAI and MMHWFM) acted on formyl peptide receptor (FPR)1 while the other peptide (MMHWAM) acted on FPR2. Since the three novel peptides were specific agonists for FPR1 or FPR2, they might be useful tools to study FPR1- or FPR2-mediated immune response and signaling.
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