Review
Biochemistry & Molecular Biology
Hao Wei, Ji-Yang Wang
Summary: Transcytosis of polymeric IgA and IgM mediated by pIgR is essential for mucosal immunity. The J chain plays a crucial role in the formation and recognition of polymeric IgA and IgM by pIgR, while MZB1 may act as a regulator to facilitate IgA and IgM transcytosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Malte Johannes Hannich, Alexander Dressel, Kathrin Budde, Astrid Petersmann, Matthias Nauck, Marie Suesse
Summary: In this study, researchers investigated the synthesis of intrathecal FLC-k in cerebrospinal fluid samples from 296 patients. They found that FLC-k synthesis can occur independently of the antibody class produced, and can help distinguish between an inflammatory process and blood contamination. This suggests that additional determination of FLC-k in quotient diagrams may aid in clinical diagnostics.
Article
Immunology
Nienke Oskam, Pleuni Ooijevaar-de Heer, Ninotska I. L. Derksen, Simone Kruithof, Steven W. de Taeye, Gestur Vidarsson, Sanne Reijm, Theresa Kissel, Rene E. M. Toes, Theo Rispens
Summary: This study systematically investigated human IgM-mediated complement activation and found that hexameric IgM outperformed pentameric IgM and IgG1 in complement activation at low antigen densities, highlighting the importance of IgM in immunity.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sven T. Sowa, Antti Moilanen, Ekaterina Biterova, Mirva J. Saaranen, Lari Lehtio, Lloyd W. Ruddock
Summary: The protein pERp1 plays a crucial role in folding IgA, IgM, integrins, and maintaining ER calcium homeostasis and plasma cell mobility. Upregulated in autoimmune diseases, pERp1 is a potential therapeutic target. Belonging to the CNPY family, these ER resident saposin-like proteins are implicated in protein folding, despite lacking lipid-binding activity typically associated with saposins. The high-resolution crystal structure of human pERp1 reveals unique structural elements and sheds light on the role of CNPY proteins in ER protein folding.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Immunology
Christopher M. Skopnik, Khlowd Al-Qaisi, Rosaleen A. Calvert, Philipp Enghard, Andreas Radbruch, Brian J. Sutton, Hiromi Kubagawa
Summary: This study identified critical amino acid residues of human Fc mu R for binding to IgM Fc, as well as the effects of certain residues on receptor expression and IgM binding. Results suggest specific sites and stretches within the Fc mu R structure that are essential for its proper function and interaction with IgM.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biology
J. Porter Hunt, Tyler J. Free, Jackelyn Galiardi, Kevin M. Watt, David W. Wood, Bradley C. Bundy
Summary: Thyroid receptor signaling plays a crucial role in physiological processes and its disruption can lead to severe disorders. Detection of thyroid receptor ligands is important for toxicology and pharmacology. A biosensor system was developed using cell-free protein synthesis and reengineered with different reporter systems, reducing the cost by 60%. This could potentially be a promising platform for high throughput screening and portable detection of human thyroid receptor ligands.
Review
Chemistry, Multidisciplinary
Huan Wang, Shiqi Lin, Xiying Wu, Kuan Jiang, Huiping Lu, Changyou Zhan
Summary: This review summarizes the intricate relationship between liposomes and their biological environments, with a focus on the regulatory effects of immunoglobulin M (IgM) on the in vivo performance of liposomes. Corresponding solutions to evade IgM-mediated opsonization for safe and efficient drug delivery are also discussed.
Article
Immunology
Xiaoxue Yin, Xiaoyu Li, Liangliang Mu, Hao Bai, Yanjian Yang, Nuo Chen, Liting Wu, Shengli Fu, Jun Li, Wantao Ying, Jianmin Ye
Summary: This study reveals the association between site-specific high-mannose modification, IgM antibody affinity, and structural disulfide polymerization. The combination of IgM and mannose receptor amplifies the phagocytic ability of phagocytes, contributing to the immune defense against microbial infection.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Reetesh Raj Akhouri, Suchi Goel, Ulf Skoglund
Summary: A study reveals that the binding of human pentameric immunoglobulin M (IgM) to VAR2CSA can inhibit the occurrence of placental malaria by creating a barrier that hinders its interaction with its receptor.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
James M. Heather, Matthew J. Spindler, Marta Herrero Alonso, Yifang Ivana Shui, David G. Millar, David S. Johnson, Mark Cobbold, Aaron N. Hata
Summary: The study and manipulation of T cell receptors (TCRs) are crucial in immunology research. Stitchr is a software tool that can generate complete TCR coding sequences based on minimal V/J/CDR3 information, increasing the speed and reproducibility of TCR research.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Maxime Fieux, Sandra Le Quellec, Sophie Battier, Andre Coste, Bruno Louis, Caroline Giroudon, Mikail Nourredine, Emilie Bequignon
Summary: FcRn has been shown to efficiently transport biologics across the nasal epithelial barrier in multiple studies reviewed. Limited studies have evaluated the potential toxicity of intranasal delivery of biologics, but they all indicate its safety. Further research is needed to fully understand the pharmacokinetic and pharmacodynamic properties of biologics after intranasal delivery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
William M. Shaw, Yunfeng Zhang, Xinyu Lu, Ahmad S. Khalil, Graham Ladds, Xiaozhou Luo, Tom Ellis
Summary: The study developed a yeast-based biosensor for screening microbial mutant libraries producing Delta 9-THC, aiming to improve production efficiency. Utilizing the cannabinoid biosensor, THC can be detected in microbial cell culture, providing a new tool for screening high producers.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Mark Chernyshev, Mateusz Kaduk, Martin Corcoran, Gunilla B. Karlsson Hedestam
Summary: This study quantified VDJ gene usage levels in the unmutated IgM repertoires of 45 macaques, revealing similar patterns of gene usage among macaque subgroups and individuals. Unlike humans, macaques showed a higher usage of IGHV4 family genes and evidence of evolutionary expansion of this gene family.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Angus Li, Rosemary Thwaite, Stuart Kellie, Andrew C. Barnes
Summary: The study identified two IgM heavy chain sub-isotypes and two light chain variants in the serum of giant grouper, purified by different methods. Through genomic contig and cDNA sequencing, the research revealed molecular differences in the IgM structure of giant grouper. Additionally, the study corrected NCBI annotations and proposed the presence of IgM sub-isotypes as a conserved trait among species of the Epinephelus genus.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Ana Carolina Serafim Vilela, Camila Alves Costa, Suzane Aparecida Oliveira, Menira Borges Lima Dias Souza, Fabiola Souza Fiaccadori, Claudio Rodrigues Leles, Nadia Lago Costa
Summary: The study assessed the accuracy of three rapid tests for detecting SARS-CoV-2 antibodies in saliva and plasma samples. The tests showed higher accuracy in plasma samples. Saliva can be used as a simple supplementary method, especially in severe and critical cases, with important sensitivity and reliability.
Article
Biochemistry & Molecular Biology
Salvatore Santamaria, Kazuhiro Yamamoto, Kenneth Botkjaer, Christopher Tape, Michael R. Dyson, John McCafferty, Gillian Murphy, Hideaki Nagase
BIOCHEMICAL JOURNAL
(2015)
Article
Pharmacology & Pharmacy
M. R. Dyson
DRUGS OF THE FUTURE
(2015)
Article
Biochemical Research Methods
Michael R. Dyson, Yong Zheng, Cunjie Zhang, Karen Colwill, Kritika Pershad, Brian K. Kay, Tony Pawson, John McCafferty
ANALYTICAL BIOCHEMISTRY
(2011)
Article
Biochemistry & Molecular Biology
Michael R. Dyson
BIOCHEMICAL SOCIETY TRANSACTIONS
(2010)
Article
Biochemical Research Methods
Ronny Falk, Anna Falk, Michael R. Dyson, Anna N. Melidoni, Kothai Parthiban, Joyce L. Young, Wendy Roake, John McCafferty
Article
Biochemical Research Methods
Karen Colwill, Susanne Graslund
Article
Biochemistry & Molecular Biology
Michael R. Dyson, Rajika L. Perera, S. Paul Shadbolt, Lynn Biderman, Krystyna Bromek, Natalia V. Murzina, John McCafferty
NUCLEIC ACIDS RESEARCH
(2008)
Article
Multidisciplinary Sciences
Anna N. Melidoni, Michael R. Dyson, Sam Wormald, John McCafferty
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2013)
Article
Biochemistry & Molecular Biology
K. Pershad, J. D. Pavlovic, S. Graslund, P. Nilsson, K. Colwill, A. Karatt-Vellatt, D. J. Schofield, M. R. Dyson, T. Pawson, B. K. Kay, J. McCafferty
PROTEIN ENGINEERING DESIGN & SELECTION
(2010)
Article
Biochemistry & Molecular Biology
Hyung-Kwon Lim, Thomas J. Mansell, Stephen W. Linderman, Adam C. Fisher, Michael R. Dyson, Matthew P. DeLisa
Article
Rheumatology
Susan R. Aungier, Alison J. Cartwright, Anja Schwenzer, Jennifer L. Marshall, Michael R. Dyson, Peter Slavny, Kothai Parthiban, Aneesh Karatt-Vellatt, Ilfita Sahbudin, Eric Culbert, Patrick Hextall, Felix I. L. Clanchy, Richard Williams, Brian D. Marsden, Karim Raza, Andrew Filer, Christopher Dominic Buckley, John McCafferty, Kim S. Midwood
ANNALS OF THE RHEUMATIC DISEASES
(2019)
Letter
Rheumatology
Susan Aungier, Alison J. Cartwright, Anja Schwenzer, Jennifer Marshall, Mike R. Dyson, Peter Slavny, Kothai Parthiban, Aneesh Karatt-Vellatt, Ilfita Sahbudin, Eric Culbert, Patrick Hextall, Felix I. L. Clanchy, Richard Williams, Brian D. Marsden, Karim Raza, Andrew Filer, Christopher D. Buckley, John McCafferty, Kim S. Midwood
ANNALS OF THE RHEUMATIC DISEASES
(2020)
Article
Medicine, Research & Experimental
Kothai Parthiban, Rajika L. Perera, Maheen Sattar, Yanchao Huang, Sophie Mayle, Edward Masters, Daniel Griffiths, Sachin Surade, Rachael Leah, Michael R. Dyson, John McCafferty
Article
Medicine, Research & Experimental
Michael R. Dyson, Edward Masters, Deividas Pazeraitis, Rajika L. Perera, Johanna L. Syrjanen, Sachin Surade, Nels Thorsteinson, Kothai Parthiban, Philip C. Jones, Maheen Sattar, Gordana Wozniak-Knopp, Florian Rueker, Rachael Leah, John McCafferty
