Article
Immunology
Faezzah Baharom, Ramiro A. Ramirez-Valdez, Kennedy K. S. Tobin, Hidehiro Yamane, Charles-Antoine Dutertre, Ahad Khalilnezhad, Glennys Reynoso, Vincent L. Coble, Geoffrey M. Lynn, Matthew P. Mule, Andrew J. Martins, John P. Finnigan, Xiao Meng Zhang, Jessica A. Hamerman, Nina Bhardwaj, John S. Tsang, Heather D. Hickman, Florent Ginhoux, Andrew S. Ishizuka, Robert A. Seder
Summary: The study demonstrates that using a self-assembling nanoparticle vaccine and adjuvant can expand stem-like CD8(+) T cells, optimize anti-tumor immunity, and enhance the strength and effectiveness of the anti-tumor response.
Article
Biochemistry & Molecular Biology
Faezzah Baharom, Ramiro A. Ramirez-Valdez, Ahad Khalilnezhad, Shabnam Khalilnezhad, Marlon Dillon, Dalton Hermans, Sloane Fussell, Kennedy K. S. Tobin, Charles-Antoine Dutertre, Geoffrey M. Lynn, Soren Muller, Florent Ginhoux, Andrew S. Ishizuka, Robert A. Seder
Summary: Therapeutic cancer vaccines aim to enhance tumor-specific T cell immunity, but suppressive mechanisms within the tumor microenvironment can limit T cell function. This study investigated how different routes of vaccination impact intratumoral myeloid cells and found that intravenous administration of a nanoparticle vaccine induced tumor regression mediated by systemic type I interferon, leading to a reduction in intratumoral monocytes expressing immune-regulatory genes. In humans, these gene signatures are associated with worse outcomes. These results suggest that combining the generation of tumor-specific CD8+ T cells with remodeling of the tumor microenvironment holds promise for tumor immunotherapy.
Article
Immunology
Ella Bhagyaraj, Hongbin Wang, Xinghong Yang, Carol Hoffman, Ali Akgul, Zakia Goodwin, David W. Pascual
Summary: znBAZ infection induces an increase and activation of lung NK cells in mice, which play a crucial role in promoting lung DC activation, migration, and the development of protective CD8(+) T cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Virology
Roberta Manco, Luciana D'Apice, Maria Trovato, Lucia Lione, Erika Salvatori, Eleonora Pinto, Mirco Compagnone, Luigi Aurisicchio, Piergiuseppe De Berardinis, Rossella Sartorius
Summary: Research shows that using filamentous bacteriophage to express peptides derived from tumor-associated antigens (TAAs) and displaying them at a high density on the viral coat proteins can enhance the immunogenicity of TAAs, triggering effective anti-tumor responses. Furthermore, expressing a CD8+ peptide derived from the human cancer germline antigen NY-ESO-1 on filamentous bacteriophages and decorating them with alpha-GalactosylCeramide (alpha-GalCer) lipid can further enhance the efficacy of anti-tumor vaccines, as demonstrated in experiments.
Article
Biochemistry & Molecular Biology
Benjamin S. Gully, Hariprasad Venugopal, Alex J. Fulcher, Zhihui Fu, Jessica Li, Felix A. Deuss, Carmen Llerena, William R. Heath, Mireille H. Lahoud, Irina Caminschi, Jamie Rossjohn, Richard Berry
Summary: DEC-205, a key receptor of dendritic cells, has a compact structure consisting of two intercalated rings of C-type lectinlike domains. The tetrameric assembly and disruption of oligomers in a cellular setting provide insights into the structural arrangement of DEC-205.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Oncology
Takayoshi Yamauchi, Toshifumi Hoki, Takaaki Oba, Ryutaro Kajihara, Kristopher Attwood, Xuefang Cao, Fumito Ito
Summary: The use of tumor mutation-derived neoantigens in cancer vaccines shows promise, but overall clinical efficacy is limited. The frequency of circulating CX3CR1(+) CD8(+) T cells and specific signaling molecules are correlated with the antitumor efficacy of neoantigen vaccines. While the CX3CR1(+) subset may not be essential for antitumor CD8(+) T cell responses, it can serve as a predictive T-cell biomarker for effective priming of CD8(+) T cells.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Marta Fortunato, Giada Amodio, Silvia Gregori
Summary: Tolerogenic dendritic cells (tolDC) play a central role in immune homeostasis and peripheral tolerance. We developed a protocol to generate genetically engineered tolDC overexpressing IL-10 (DCIL-10) and found that they can modulate cytotoxic CD8(+) T cell responses by reducing proliferation and activation, and inducing anergic CD8(+) T cells without signs of exhaustion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Yana Hackler, Frank Siebenhaar, Max Loehning, Marcus Maurer, Melba Munoz
Summary: MCs play a critical role in the activation and expansion of virus-specific CD8(+) T cells through proper dendritic cell (DC) activation, and are essential for antiviral functions during viral infections. Depletion of MCs impairs CD8(+) T cell effector phenotype, antiviral cytokine production, and DC activation, resulting in increased viral loads and reduced type-I interferon levels.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Bingjie Zhang, Rabi Upadhyay, Yuhan Hao, Marie I. Samanovic, Ramin S. Herati, John D. Blair, Jordan Axelrad, Mark J. Mulligan, Dan R. Littman, Rahul Satija
Summary: This study analyzed the T cell response after BNT162b2 vaccination using multimodal sequencing technologies. The researchers identified CD8(+) T cell subpopulations and explored their transcriptome, chromatin landscape, and immunophenotype. They also found that the frequency and differentiation outcomes of these CD8(+) T cell subpopulations were predictive of clinical outcomes in COVID-19 patients.
Article
Immunology
Adele Friot, Sophia Djebali, Severine Valsesia, Peggy Parroche, Maxence Dubois, Jessica Baude, Francois Vandenesch, Jacqueline Marvel, Yann Leverrier
Summary: Staphylococcus aureus is a pathogen associated with various diseases and the emergence of antibiotic-resistant strains has raised concerns. Developing vaccines may help overcome these resistant strains, but the ability of S. aureus to internalize into cells poses a challenge. This study explores the potential of CD8(+) T cells to recognize and kill S. aureus-infected dendritic cells, paving the way for CD8(+) T cell-based therapies against S. aureus.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Spencer E. E. Brightman, Angelica Becker, Rukman R. R. Thota, Martin S. S. Naradikian, Leila Chihab, Karla Soria Zavala, Ryan Q. Q. Griswold, Joseph S. S. Dolina, Ezra E. W. Cohen, Aaron M. M. Miller, Bjoern Peters, Stephen P. P. Schoenberger
Summary: CD4(+) T cells have important roles in immune responses, either directly or through accessory cells such as CD8(+) T lymphocytes. While the role of NeoAg-specific CD8(+) T cells in cancer has been extensively studied, the role of NeoAg-specific CD4(+) T cells is less well understood.
Article
Immunology
Tyler G. Normile, Antonella Rella, Maurizio Del Poeta
Summary: The study found that a C. neoformans mutant, Delta sgl1, accumulating sterylglucosides (SGs), can provide complete protection under immunodeficient conditions. The mutant decreases levels of SGs post-fungal clearance.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Gastroenterology & Hepatology
Tobias Boettler, Benedikt Csernalabics, Henrike Salie, Hendrik Luxenburger, Lara Wischer, Elahe Salimi Alizei, Katharina Zoldan, Laurenz Krimmel, Peter Bronsert, Marius Schwabenland, Marco Prinz, Carolin Mogler, Christoph Neumann-Haefelin, Robert Thimme, Maike Hofmann, Bertram Bengsch
Summary: This case report highlights the occurrence of autoimmune hepatitis episodes following COVID-19 vaccination in a patient with distinctive T cell-dominant immune-mediated hepatitis. Liver tissue analysis revealed an immune infiltrate with activated cytotoxic CD8 T cells predominantly distributed, indicating a unique pathomechanism associated with vaccination-induced antigens.
JOURNAL OF HEPATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ferran Soldevila, Jane C. Edwards, Simon P. Graham, Helen R. Crooke, Dirk Werling, Falko Steinbach
Summary: The study found that the C-strain vaccine caused changes in the myeloid cell compartment of the tonsil, leading to an increase and activation of specific immune cells. Additionally, the C-strain vaccine also promoted the activation of conventional dendritic cells 1 (cDC1) in the tonsil, which coincided with the induction of CSFV-specific CD8 T cell responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
N. Balneger, L. A. M. Cornelissen, M. Wassink, S. J. Moons, T. J. Boltje, Y. E. Bar-Ephraim, K. K. Das, J. N. Sondergaard, C. Bull, G. J. Adema
Summary: The study reveals that sialic acids play a crucial role in regulating the activation and interaction of dendritic cells. Blocking sialic acids enhances the immune activation ability and antigen-induced T cell interactions of dendritic cells. These findings have important implications for understanding the mechanisms of immune responses.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Immunology
Valentina Poli, Marco Di Gioia, Martha Sola-Visner, Francesca Granucci, Andrew L. Frelinger, Alan D. Michelson, Ivan Zanoni
Summary: Thrombin signaling induces NFAT activation in platelets, and inhibiting NFAT enhances platelet activation, aggregation, and interactions with neutrophils. NFAT inhibition promotes disease severity during gram-negative septicemia by increasing disseminated coagulation and NETosis. These findings provide new therapeutic targets for clinical needs.
Review
Chemistry, Multidisciplinary
Esmaeel Sharifi, Ashkan Bigham, Satar Yousefiasl, Maria Trovato, Matineh Ghomi, Yasaman Esmaeili, Pouria Samadi, Ali Zarrabi, Milad Ashrafizadeh, Shokrollah Sharifi, Rossella Sartorius, Farnaz Dabbagh Moghaddam, Aziz Maleki, Hao Song, Tarun Agarwal, Tapas Kumar Maiti, Nasser Nikfarjam, Colin Burvill, Virgilio Mattoli, Maria Grazia Raucci, Kai Zheng, Aldo R. Boccaccini, Luigi Ambrosio, Pooyan Makvandi
Summary: Mesoporous bioactive glasses (MBGs) as a multifunctional platform have shown promising potential as theranostic systems for cancer imaging and therapy. While MBGs have not fully reached their potential in practice, recent research has highlighted their excellent capabilities in the field of tumor treatment and imaging.
Article
Biochemistry & Molecular Biology
Federica Farina, Laura Pisapia, Mariavittoria Laezza, Gloria Serena, Antonio Rispo, Simona Ricciolino, Carmen Gianfrani, Alessio Fasano, Giovanna Del Pozzo
Summary: Macrophages play a crucial role in the development of celiac disease by expressing CD-associated HLA-DQ2.5 risk alleles differently from other antigen-presenting cells. Gliadin stimulation increases the expression of DQA1*05:01 and decreases the expression of DQB1*02:01 in macrophages, along with downregulation of DRB1 genes and CIITA transactivator.
Review
Cell Biology
P. Sahu, A. Balakrishnan, R. Di Martino, A. Luini, D. Russo
Summary: Tumorigenesis is associated with aberrant glycosylation, but the exact cause and mechanisms are still unclear. The levels and localization of glycosylation enzymes determine the glycosylation potential, and the cisternal maturation mechanism plays a key role in Golgi glycan synthesis. This mechanism is proposed to be a combination of pathways that control functionally related glycosylation enzymes. The identification of oncogenes among the cisternal maturation machinery provides insights into the regulation of aberrant glycosylation in cancer cells and potential targets for anticancer treatments.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Simona Del Giudice, Valentina De Luca, Seyedehnegar Parizadeh, Domenico Russo, Alberto Luini, Rosaria Di Martino
Summary: This article reviews the autoregulatory circuits acting on the Golgi complex, with a focus on the role of specific signaling molecules in cancer. It proposes to draw awareness to the Golgi-localized regulatory systems as potential targets in cancer therapy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Esmaeel Sharifi, Seyede Athar Sadati, Satar Yousefiasl, Rossella Sartorius, Mahdi Zafari, Leila Rezakhani, Morteza Alizadeh, Ehsan Nazarzadeh Zare, Shadi Omidghaemi, Fatemeh Ghanavatinejad, Mohammad-Saeid Jami, Erfan Salahinejad, Hadi Samadian, Ana Claudia Paiva-Santos, Piergiuseppe De Berardinis, Abbas Shafiee, Franklin R. Tay, Samiramis Pourmotabed, Pooyan Makvandi
Summary: This study developed a tissue-engineered dermal substitute with antibacterial and wound healing-promoting properties. The substitute consisted of nanofibrous scaffolds and silver-doped glass-ceramic. The substitute exhibited good biocompatibility and hemocompatibility, promoting cell attachment and proliferation without inducing an inflammatory response. The degradation rate of the substitute matched the rate of skin regeneration under in vivo conditions. In a mouse model, the substitute promoted angiogenesis, collagen synthesis, and regeneration of sebaceous glands and hair follicles.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Editorial Material
Immunology
Jens Geginat, Francesca Granucci
Summary: Regulatory T-cells (Tregs) are crucial for preventing autoimmunity and also play a role in immune stimulation. A recent study discovered that Tregs are a physiological source of IL-15, which is important for maintaining CD8(+) memory T-cells.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Valentina Poli, Ivan Zanoni
Summary: Neutrophil extracellular traps (NETs) are formed by a web-like structure of DNA and serve to protect the host against microbial infections, but they can also cause tissue damage and contribute to inflammatory diseases. The induction, release, and degradation of NETs must be tightly regulated. This article discusses the intrinsic pathways in neutrophils that regulate NETosis and reviews the latest findings on how extrinsic factors from bystander cells contribute to NET formation. The potential of harnessing these extrinsic processes to control excessive inflammation caused by uncontrolled NET release is also discussed.
TRENDS IN MICROBIOLOGY
(2023)
Article
Surgery
Jifu Ge, Weikang Pan, Noel J. Feeney, Leah Ott, Emily Anderson, Alessandro Alessandrini, Ivan Zanoni, James F. Markmann, Alex G. Cuenca
Summary: Advances in immunosuppression have been limited, and long-term morbidity associated with immunosuppression regimens remains a problem for transplant recipients. A novel administration strategy using the adjuvant alum was discovered, which expanded MDSCs and suppressed T cell proliferation. These MDSCs also facilitated the differentiation of thorn T cells into regulatory T cells. Adjuvant treatment significantly delayed alloislet rejection, and the increased IL-10 production by AC MDSCs played a critical role in their suppressor function and protective effect. Therapeutics aimed at expanding MDSCs could be a useful strategy to prevent transplant rejection and reduce the use of toxic immunosuppressive regimens.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Virology
Andrea Del Mastro, Stefania Picascia, Luciana D'Apice, Maria Trovato, Pasquale Barba, Immacolata Di Biase, Sebastiano Di Biase, Marco Laccetti, Antonello Belli, Gerardino Amato, Potito Di Muro, Olga Credendino, Alessandra Picardi, Piergiuseppe De Berardinis, Giovanna Del Pozzo, Carmen Gianfrani
Summary: Kidney transplanted recipients (KTR) are at high risk of severe SARS-CoV-2 infection due to immunosuppressive therapy. Studies analyzed the immune response in KTR and healthy controls after the second and third dose of the mRNA vaccine. The third dose increased neutralizing antibody titers in both groups, but KTR had lower levels and showed low immunity to Omicron variant.
Article
Virology
Roberta Manco, Luciana D'Apice, Maria Trovato, Lucia Lione, Erika Salvatori, Eleonora Pinto, Mirco Compagnone, Luigi Aurisicchio, Piergiuseppe De Berardinis, Rossella Sartorius
Summary: Research shows that using filamentous bacteriophage to express peptides derived from tumor-associated antigens (TAAs) and displaying them at a high density on the viral coat proteins can enhance the immunogenicity of TAAs, triggering effective anti-tumor responses. Furthermore, expressing a CD8+ peptide derived from the human cancer germline antigen NY-ESO-1 on filamentous bacteriophages and decorating them with alpha-GalactosylCeramide (alpha-GalCer) lipid can further enhance the efficacy of anti-tumor vaccines, as demonstrated in experiments.
Article
Chemistry, Multidisciplinary
Rezvan Jamaledin, Rossella Sartorius, Concetta Di Natale, Valentina Onesto, Roberta Manco, Valentina Mollo, Raffaele Vecchione, Piergiuseppe De Berardinis, Paolo Antonio Netti
Summary: Bacteriophages have been widely studied in various research areas such as tissue engineering and therapeutic applications. Encapsulation of bacteriophages in polymeric microparticles can improve their stability and release control. This study demonstrated that encapsulated bacteriophages preserved their structure, remained immunologically active, and had prolonged stability. Furthermore, in silico predictions were used to regulate the release of bacteriophages. These findings lay the foundation for a versatile bacteriophage-based vaccine delivery system with sustained immune response.
JOURNAL OF NANOSTRUCTURE IN CHEMISTRY
(2023)
Review
Nanoscience & Nanotechnology
Atefeh Malek-Khatabi, Zahra Tabandeh, Akram Nouri, Elaheh Mozayan, Rossella Sartorius, Shahnaz Rahimi, Rezvan Jamaledin
Summary: Biodegradable polymers are widely used in the biomedical field due to their high biocompatibility and negligible toxicity. This review introduces the use of different biodegradable polymers and their degradation mechanism, highlights different types of vaccines and their interaction with the immune system, and discusses the application of natural and synthetic biodegradable micro-/nanoplatforms, hydrogels, and scaffolds for local or targeted and controlled vaccine release.
ACS APPLIED BIO MATERIALS
(2022)