Article
Biochemistry & Molecular Biology
Pinduo Liu, Anping Wu, Hui Li, Jun Zhang, Junjun Ni, Zhenzhen Quan, Hong Qing
Summary: This study revealed the degradation pathways of Rab21 protein through the ubiquitin-proteasome pathway and the autophagy-lysosome pathway, and demonstrated increased ubiquitination of Rab21 protein in the AD model. Moreover, the study suggested the involvement of the autophagy-lysosome pathway in maintaining the protein level of Rab21.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
M. Roths, M. A. Abeyta, B. Wilson, T. E. Rudolph, M. B. Hudson, R. P. Rhoads, L. H. Baumgard, J. T. Selsby
Summary: Heat stress does not increase skeletal muscle proteolysis, contrary to previous beliefs, which calls into question the assumption that increased skeletal muscle proteolysis drives increased amino acid mobilization.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Microbiology
Bingmei Dong, Feng Li, Jinliang Wang, Sufang Lv, Lizhong Miao, Guangjun Guo, Zhiqiang Shen
Summary: This study investigated the roles of ubiquitin-proteasome system (UPS) and autophagy-lysosome pathway (ALP) in Brucella.suis (B.suis) infection in murine macrophages. The results showed that B.suis activated ALP and UPS, and both pathways contributed to the intracellular proliferation of B.suis. Furthermore, the study demonstrated that ALP was activated following UPS inhibition, and UPS had a stronger ability than ALP in promoting intracellular proliferation of B.suis.
VETERINARY MICROBIOLOGY
(2023)
Review
Cell Biology
Sara E. Hanley, Katrina F. Cooper
Summary: Protein homeostasis is maintained by three major pathways including the ubiquitin-proteasome system, autophagy-lysosomal pathway, and endo-lysosomal pathway. Sorting nexins, a family of membrane-associated proteins, play important roles in protein trafficking and maintaining protein quality control mechanisms by connecting with ubiquitin. Defects in sorting nexins are associated with a range of human diseases, highlighting their critical roles in cellular function.
Article
Biochemistry & Molecular Biology
Ajay R. Wagh, Prasad Sulakshane, Michael H. Glickman
Summary: Misfolded protein aggregation is the main cause of Alzheimer's disease. The accumulation of UBB+1 in extracellular plaques suggests a role of the ubiquitin-proteasome system in Alzheimer's disease. The mechanism of extracellular secretion of UBB+1 remains unknown.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2023)
Review
Biochemistry & Molecular Biology
Na Yang, Bo Kong, Zhaohong Zhu, Fei Huang, Liliang Zhang, Tao Lu, Yadong Chen, Yanmin Zhang, Yulei Jiang
Summary: Targeted protein degradation (TPD) technology, such as PROTACs, has become widespread in the past 20 years and greatly accelerates the development of disease treatment. Unlike small inhibitors, targeted protein degraders can target undruggable targets and overcome drug resistance through ubiquitin-proteasome pathway (UPP) and lysosome pathway. This review discusses different degradation technologies and provides a basis for future research.
MOLECULAR DIVERSITY
(2023)
Review
Biochemistry & Molecular Biology
Ah Jung Heo, Su Bin Kim, Yong Tae Kwon, Chang Hoon Ji
Summary: The N-degron pathway is a degradative system that regulates protein and biological material half-lives through N-terminal amino acids called N-degrons. N-degrons are recognized by N-recognins, which link them to the ubiquitin-proteasome system (UPS) or autophagy-lysosome system (ALS). The UPS targets N-terminal arginine (Nt-Arg) and other N-degrons for proteasomal proteolysis, while the ALS induces degradation of substrates and various cargoes through N-recognition. This interaction involves reprogramming of the Ub code. Eukaryotic cells have developed diverse mechanisms for targeting all 20 amino acids for degradation. This article discusses the components, regulation, and functions of N-degron pathways, with a focus on Arg/N-degrons and N-recognins' basic mechanisms and therapeutic applications.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2023)
Review
Biochemistry & Molecular Biology
Yaping Liu, Runrong Ding, Ze Xu, Yuan Xue, Dongdong Zhang, Yujing Zhang, Wenjie Li, Xing Li
Summary: Alzheimer's disease is characterized by the formation of senile plaques and neurofibrillary tangles in the brain, but these pathologic biomarkers can only be detected after a sustained disease progression. The challenge in AD treatment is the lack of effective biomarkers for early diagnosis. The protein quality control system plays a crucial role in AD, as the accumulation of abnormal proteins is closely related to its dysfunction. Therefore, focusing on the protein quality control system can provide novel therapeutic strategies for preventing AD development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Olga Sokolova, Gunter Maubach, Michael Naumann
Summary: Helicobacter pylori infection induces a strong inflammatory response in gastric mucosa and alters epithelial integrity and function. The activation of NF-κB transcription factors, specifically by the bacterial heptose metabolites, triggers this innate immune response. Our study reveals that the activation of ALPK1-dependent TIFA in H. pylori-infected gastric epithelial cells is followed by TIFA degradation, which is inhibited by proteasomal and lysosomal inhibitors. TRAF2, TRAF6, TAK1, or NEMO are not involved in TIFA degradation, and H. pylori promotes the interaction of TIFA with protein adaptors involved in lysosomal trafficking.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2023)
Review
Pharmacology & Pharmacy
Huanjie Zhu, Jin Wang, Qingqing Zhang, Xiaoyan Pan, Jie Zhang
Summary: Targeted Protein Degradation (TPD) is a powerful technique for designing and treating new drugs, particularly in overcoming drug resistance in cancer. However, traditional PROTACs have shown potential disadvantages, such as poor oral bioavailability and pharmacokinetics. Scientists are now focused on developing new TPD technologies to overcome these drawbacks.
PHARMACOLOGY & THERAPEUTICS
(2023)
Article
Cell Biology
Yusuke Nishimura, Jitpisute Chunthorng-Orn, Samuel Lord, Ibrahim Musa, Peter Dawson, Lars Holm, Yu-Chiang Lai
Summary: In this study, the researchers showed that Atrogin-1 and MuRF1 protein contents are regulated by different mechanisms, specifically the downstream of Akt, and that Atrogin-1 protein content can be regulated by the rapamycin-sensitive mTOR-S6K1-dependent signaling pathway.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Microbiology
Priyadharshini Ramachandran, J. Beslin Joshi, Julie A. Maupin-Furlow, Sivakumar Uthandi
Summary: Plant pathogenic Gram-negative bacteria evade the host plant immune system by secreting Type III and Type IV effector proteins, which mimic components of the ubiquitin-265 proteasome system to control plant cellular activities and establish pathogenicity.
MICROBIOLOGICAL RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Deepa Kumari, Jeffrey L. Brodsky
Summary: Protein quality control processes are crucial for cellular and organism health, particularly for polypeptides entering the endoplasmic reticulum (ER). ER-associated degradation (ERAD) is a multi-step pathway that targets inefficiently matured proteins for degradation, providing a mechanism to regulate protein levels and activities in the ER.
Review
Environmental Sciences
Amit Gupta, Tapan Behl, Lotfi Aleya, Md. Habibur Rahman, Harlokesh Narayan Yadav, Giridhari Pal, Ishnoor Kaur, Sandeep Arora
Summary: Type 2 diabetes is a common metabolic disorder, and therapies targeting UPP pathway may have promising results in treating diabetes and associated complications. The role of ubiquitin molecules extends beyond neurodegenerative disorders to play a critical role in diabetes progression.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Mikhail Rudinskiy, Maurizio Molinari
Summary: Conserved catabolic pathways operate to remove aberrant polypeptides from the endoplasmic reticulum (ER), utilizing ERAD pathways and ER-phagy. ERAD pathways control retrotranslocation of misfolded proteins across the ER membrane, while ER-phagy segregates proteins into ER subdomains that eventually vesiculate. These ER-derived vesicles can be captured by autophagosomes, endolysosomes/vacuoles, or fused with degradative organelles.
Article
Cardiac & Cardiovascular Systems
Marisol Gouveia, Cristine Schmidt, Manuel Teixeira, Mario Lopes, Susana S. Aveiro, Pedro Domingues, Ke Xia, Wilfredo Colon, Rui Vitorino, Rita Ferreira, Mario Santos, Sandra Vieira, Fernando Ribeiro
Summary: This study characterizes the plasma levels and composition of SDS-resistant aggregates (SRAs) in patients with heart failure with preserved ejection fraction (HFpEF) and identifies the molecular pathways associated with disease and/or proteostasis disruption. The study found that patients with HFpEF have higher levels of SRAs, which are composed of carbonylated and ubiquitinated proteins and enriched in proteins related to cardiovascular function/disease and proteostasis machinery. Furthermore, the study suggests that the reduced proteostasis capacity in HFpEF may contribute to the higher accumulation of SRAs.
JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH
(2023)
Review
Sport Sciences
Laura Freitas, Andrea Bezerra, Tania Amorim, Ricardo J. Fernandes, Jose Duarte, Helder Fonseca
Summary: This study analyzed the effect of swimming on bone mass, cortical geometry, and trabecular microarchitecture. The results showed that swimmers have lower bone mass compared to athletes engaged in high-impact sports, but similar to those engaged in moderate- and non-impact sports as well as non-athletes. Swimmers' cortical geometry and trabecular microarchitecture were also found to be similar to those of athletes and non-athletes. However, the evidence was of poor quality.
GERMAN JOURNAL OF EXERCISE AND SPORT RESEARCH
(2023)
Article
Food Science & Technology
Manyou Yu, Irene Gouvinhas, Maria J. Pires, Maria J. Neuparth, Rui M. Gil da Costa, Rui Medeiros, Margarida M. S. M. Bastos, Helena Vala, Luis Felix, Carlos Venancio, Ana I. R. N. A. Barros, Paula A. Oliveira
Summary: A study found that pomegranate leaf infusion has anti-neoplastic and toxicological properties in transgenic mice carrying HPV16 oncogenes. It can reduce lesions in the liver, kidney, and skin. This suggests that pomegranate leaf infusion may have potential in preventing and treating HPV16-induced lesions, but further research is needed for confirmation.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Review
Sport Sciences
Rita Pinho Ferreira, Jose Alberto Duarte
Summary: Skeletal muscle is a highly adaptable tissue that can change its mass and function in response to various stimuli. Protein synthesis and breakdown, regulated by multiple signaling pathways, play a crucial role in skeletal muscle mass. Different signaling pathways, such as Akt/mTOR signaling, ubiquitin-proteasome pathway, and autophagy, contribute differently to protein turnover and muscle mass depending on the condition and type of muscle. PGC-1α, by modulating mitochondria biogenesis, influences the cell's bioenergetic status and protein turnover. This review aims to provide an updated understanding of the molecular pathways involved in protein turnover regulation in skeletal muscle, with a focus on how lifestyle interventions, particularly exercise training, modulate various muscle proteins. An in-depth analysis of the anabolic effects of exercise programs will pave the way for personalized management of muscle wasting conditions.
INTERNATIONAL JOURNAL OF SPORTS MEDICINE
(2023)
Article
Oncology
Rui Vitorino, Antonio S. Barros, Sofia Guedes, Douglas C. Caixeta, Robinson Sabino-Silva
Summary: NIR spectroscopy is a valuable tool for cancer diagnosis and treatment. It reveals inherent differences between tumor and normal tissue and provides insights into cancer patterns and physiology. The integration of NIR spectroscopy with advanced algorithms makes it a cutting-edge technology with great potential in medical applications.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2023)
Article
Biochemical Research Methods
Maria J. Cardoso Ferreira, Tania Soares Martins, Steven R. Alves, Ilka Martins Rosa, Jonathan Vogelgsang, Niels Hansen, Jens Wiltfang, Odete A. B. da Cruz e Silva, Rui Vitorino, Ana Gabriela Henriques
Summary: Aging is the main risk factor for age-related neurodegenerative diseases, such as Alzheimer's disease (AD), which is characterized by the presence of senile plaques (SPs) and neurofibrillary tangles (NFTs). This study conducted a literature review to identify the proteomes of SPs and NFTs, followed by a bioinformatic analysis and validation of potential biomarkers.
Article
Biochemistry & Molecular Biology
Sofia Reis Brandao, Ana Reis-Mendes, Margarida Duarte-Araujo, Maria Joao Neuparth, Hugo Rocha, Felix Carvalho, Rita Ferreira, Vera Marisa Costa
Article
Biochemistry & Molecular Biology
Andreia F. R. Silva, Rita Silva-Reis, Rita Ferreira, Paula A. Oliveira, Ana I. Faustino-Rocha, Maria de Lurdes Pinto, Manuel A. Coimbra, Artur M. S. Silva, Susana M. Cardoso
Summary: The World Health Organization aims to prevent diabetes through diet, and incorporating resveratrol (RSV) into bread is a suitable way to make it more accessible. This study evaluated the effect of RSV-enriched bread in preventing early type 2 diabetes cardiomyopathy. The results showed that an RSV bread diet can decrease polydipsia and body weight loss in the early stages of the disease.
Review
Biochemistry & Molecular Biology
Mafalda Barbosa Pedrosa, Samuel Barbosa, Rui Vitorino, Rita Ferreira, Daniel Moreira-Goncalves, Lucio Lara Santos
Summary: Chemotherapy exacerbates skeletal-muscle wasting in cancer patients, but the molecular effects of chemotherapy on muscle have not been fully understood, making the development of new anti-wasting strategies difficult. Therefore, further investigation on molecular mechanisms and clinical studies are necessary.
Article
Cell Biology
Javad Alizadeh, Xiaohui Weng, Joadi Jacobs, Shahrokh Lorzadeh, Amir Ravandi, Rui Vitorino, Stevan Pecic, Aleksandra Zivkovic, Holger Stark, Shahla Shojaei, Saeid Ghavami
Summary: This article discusses the role of ceramide synthases and ceramides in regulating apoptosis and autophagy in cancer cells. It highlights the context-dependent mechanisms of regulation and the potential applications in cancer therapies.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2023)
Editorial Material
Biochemistry & Molecular Biology
Rui Vitorino
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Giulia Guadalupi, Cristina Contini, Federica Iavarone, Massimo Castagnola, Irene Messana, Gavino Faa, Simona Onali, Luchino Chessa, Rui Vitorino, Francisco Amado, Giacomo Diaz, Barbara Manconi, Tiziana Cabras, Alessandra Olianas
Summary: This study investigated the quantitative variations on the salivary proteome in autoimmune hepatitis (AIH) and primary biliary cholangitis (PBC) patients. Several differentially expressed proteins related to the diseases were identified. Machine learning analysis confirmed the potential of the salivary proteome to discriminate AIH and PBC patients. The functional enrichment analysis highlighted the involvement of the immune system, liver fibrosis process, and antimicrobial activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Rita Ferreira, Francisco Amado, Rui Vitorino
Summary: Bioinformatics plays a critical role in advancing peptidomics by providing powerful tools for accelerating peptide discovery, understanding their functions, and gaining insights into complex molecular interactions.
Article
Health Care Sciences & Services
Ana Cristina Correa Figueira, Ana Pereira, Luis Leitao, Rita Ferreira, Paula A. Oliveira, Jose Alberto Duarte
Summary: The purpose of this study is to determine the severity of cancer-induced muscle wasting in different types of muscles and to investigate whether exercise training can attenuate this muscle dysfunction. The results showed that the cross-sectional area of the gastrocnemius muscle in sedentary tumor-bearing animals significantly decreased, and interstitial fibrosis increased. Exercise training was found to prevent this muscle dysfunction.
Article
Pharmacology & Pharmacy
Vera Marisa Costa, Joao Paulo Capela, Maria Lourdes Bastos, Fernando Remiao, Kurt James Varner, Jose Alberto Duarte, Felix Carvalho
Summary: Prolonged overexposure to catecholamines causes toxicity due to continuous adrenoceptor stimulation, autoxidation, and reactive pro-oxidant species formation. This study investigated the contribution of oxidative stress in adrenaline-induced neurotoxicity using non-differentiated SH-SY5Y cells. Results showed that Adrenaline caused concentration- and time-dependent cytotoxicity in the cells, and N-acetyl-cysteine prevented the toxicity while the antioxidant Tiron was non-protective. Further analysis is needed to understand the involvement of these processes in catecholamine-induced peripheral neuropathy.
BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
