期刊
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
卷 232, 期 2, 页码 231-240出版社
SPRINGER
DOI: 10.1007/s00217-010-1382-8
关键词
Bifidobacterium animalis; Exopolysaccharides; Composition; Antioxidant activity
资金
- China Agricultural University [KYCX2010062]
- China National 863 Program [2008AA10Z324]
- Beijing Natural Science Foundation [5072025]
The major exopolysaccharide fraction (EPSa) of Bifidobacterium animalis RH was purified to illustrate the chemical characterization and the antioxidant activity in vitro. The molecular weight of EPSa was 2.31 x 10(4) Da determined by the gel permeation chromatography (GPC). Fourier-transform infrared spectra (FT-IR) and the monosaccharide analysis of high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) showed that it was a heteropolysaccharide mainly composed of d-glucose, d-mannose, d-galactose, l-arabinose, d-fructose, and l-rhamnose in molar ratios of 43:34:18:4:1:1. In antioxidant assays, the EPSa had a higher activity for antilipid peroxidation and a stronger scavenging activity of hydroxyl radical and superoxide radical than ascorbic acid. It also had a similar alpha,alpha-diphenyl-beta-picrylhydrazyl (DPPH) radical-scavenging activity with ascorbic acid at low concentration and had concentration-dependent inhibitory ability on erythrocyte hemolysis. These results indicated that the EPSa fraction had strong antioxidant activities and could be explored as novel potential antioxidants.
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