期刊
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
卷 229, 期 3, 页码 533-538出版社
SPRINGER
DOI: 10.1007/s00217-009-1075-3
关键词
Biogenic amines; Decarboxylation broth; Ion-exchange chromatography; Lactic acid bacteria; PCR
资金
- Ministry of Education, Youth and Sports of the Czech Republic [MSM7088352101, MSM0021622416]
The aim of this paper was to study the biogenic amines (histamine, tyramine, putrescine, cadaverine, agmatine, spermine and spermidine) production of selected technological important lactic acid bacteria (strains of the genera Lactococcus, Lactobacillus and Streptococcus). Three methods (ion-exchange chromatography (IEC), PCR and cultivation method with pH indicator) were used. Within the 39 strains of lactic acid bacteria tested, the production of tyramine (formed by tyrosine decarboxylase) was detected in eight strains (3 strains of Lactococcus lactis subsp. lactis, three strains of Lactococcus lactis subsp. cremoris, 1 strain of Streptococcus thermophilus and 1 strain of Lactobacillus delbrueckii subsp. bulgaricus). The other tested biogenic amines were not detected. Cultivation in decarboxylation broth seems to be the least accurate method for the detection of biogenic amines due to enhanced risk of false-positive reactions. Therefore, in order to detect bacteria producing biogenic amines, the combination of PCR and chromatographic methods (e.g. IEC) can be recommended.
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