Article
Plant Sciences
Ramadan A. Arafa, Said M. Kamel, Dalia Taher, Svein O. Solberg, Mohamed T. Rakha
Summary: Late blight-resistant wild tomato produces a higher number of volatile organic compounds (VOCs) compared to susceptible genotypes. This study provides promising insights for the management of late blight and suggests that metabolic profiling can contribute to a better understanding of resistance mechanisms.
Article
Agronomy
Palanisamy Muthukumar, Gograj Singh Jat, Pritam Kalia, Chitra Devi Pandey, Raman Selvakumar, Suman Lata, Shilpi Kumari, Sachin Kumar, Bhoopal Singh Tomar
Summary: This study aimed to screen wild tomato Solanum habrochaites accessions for resistance to late blight and characterize their morphological characters. Three accessions (LA1777, LA2167, and LA2556) were found to be resistant to late blight. LA1777 showed the highest resistance and could be used as a source for breeding blight-resistant tomatoes. Hybrid combinations using LA1777 as a male parent also showed encouraging results. The morphological characterization of the accessions provided valuable information for breeding superior tomato cultivars.
GENETIC RESOURCES AND CROP EVOLUTION
(2023)
Article
Agronomy
Pasquale Tripodi, Antonella Vitiello, Bruno D'Onofrio, Mario Parisi, Maria Cammareri
Summary: Exotic libraries have been a powerful tool for exploring wild relatives and quantitative trait loci detection in crop species, with an introgression line population of wild tomato Solanum habrochaites (SH) acc. LA1777 developed in the early 2000s. Evaluation of 19 traits on a set of 39 ILs grown in three field seasons showed lower productivity compared to control genotypes, with major QTLs increasing soluble solids, pericarp thickness, and trichome density identified on specific chromosomes. The findings suggest the potential of wild tomatoes like SH for novel allele discovery and breeding purposes.
Article
Microbiology
Artemii A. Ivanov, Egor O. Ukladov, Tatiana S. Golubeva
Summary: Phytophthora infestans is a major pathogen in agriculture, with significant impact on Solanaceae species like potatoes and tomatoes. Research shows annual crop losses caused by this pathogen, particularly affecting potatoes. Further studies are needed to delve deeper into this interaction.
Article
Biochemistry & Molecular Biology
Chunxin Liu, Yiyao Zhang, Yinxiao Tan, Tingting Zhao, Xiangyang Xu, Huanhuan Yang, Jingfu Li
Summary: The SlMYBS2 gene is a positive regulator of tomato resistance to P. infestans infection by regulating reactive oxygen species (ROS) levels and pathogenesis-related (PR) gene expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Amira A. Al Harethi, Qais Y. M. Abdullah, Hala J. Al Jobory, Samar A. Al Aquil, Ramadan A. Arafa
Summary: This study successfully isolated and identified Phytophthora infestans in Yemen using advanced methods, providing fundamental data for the prevention and control of late blight.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Xiaoqing Huang, Ziyue You, Yang Luo, Chengji Yang, Jie Ren, Yanlin Liu, Guangjing Wei, Pan Dong, Maozhi Ren
Summary: Chitosan shows significant inhibition on the mycelial growth and spore germination of Phytophthora infestans, reducing its resistance to adverse conditions and having synergistic effects with pesticides. It also induces resistance in potatoes to the pathogen, with transcriptome analysis showing effects on cell growth and key pathways. This study provides new insights for the development of chitosan as an eco-friendly solution for controlling potato late blight.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Agriculture, Multidisciplinary
Suo-meng Dong, Shao-qun Zhou
Summary: This article summarizes the molecular pathogenesis of potato late blight and current control strategies, emphasizing the importance of continuous improvement in efficient and sustainable management.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Biochemistry & Molecular Biology
Kai-Ting Fan, Yang Hsu, Ching-Fang Yeh, Chi-Hsin Chang, Wei-Hung Chang, Yet-Ran Chen
Summary: This study used a data-independent acquisition (DIA) proteomics approach to investigate the dynamic regulation of tomato proteins by Phytophthora infestans at different stages of pathogenesis. It revealed the comprehensive regulation of tomato proteins involved in immunity, signaling, defense, and metabolism pathways during P. infestans infection. Several proteins related to plant defense responses were found to be differentially regulated at the transcriptional or translational levels across different pathogenesis phases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Agronomy
Weizhen Wang, Youhua Long
Summary: Phytophthora infestans causes significant economic impact on agriculture by causing late blight on potatoes and tomatoes. The application of synthetic fungicides for late blight management is not a sustainable solution. Biocontrol strategies show potential but face challenges in field applications.
PEST MANAGEMENT SCIENCE
(2023)
Article
Plant Sciences
Xinyi Hu, Kristian Persson Hoden, Zhen Liao, Anna Asman, Christina Dixelius
Summary: Phytophthora spp. cause damage to plants by using effector proteins and small RNAs. The study found that the sRNA encoded by P. infestans can affect potato mRNA, expanding our understanding of the strategies used by this species to facilitate infection.
Article
Plant Sciences
Haishan Zhu, Minghua Deng, Zhengan Yang, Lianzhen Mao, Shurui Jiang, Yanling Yue, Kai Zhao
Summary: This study identified 36 differentially expressed genes, including nucleotide binding site-leucine-rich repeat and pathogenesis-related disease resistance genes, in highly resistant and susceptible tomato inbred lines using transcriptome sequencing. Two pathogenesis-related genes, Solyc08g080660 and Solyc08g080670, were found to have unique responses to Phytophthora infestans and were confirmed to play roles in late blight resistance through gene overexpression and editing techniques. Additionally, co-transformation of both genes showed a greater effect on disease resistance compared to individual genes.
Article
Biochemistry & Molecular Biology
Ruiling Li, Alex Maioli, Zhe Yan, Yuling Bai, Danila Valentino, Anna Maria Milani, Valerio Pompili, Cinzia Comino, Sergio Lanteri, Andrea Moglia, Alberto Acquadro
Summary: The knockout of the PMR4 gene in tomato can reduce susceptibility to Phytophthora infestans and provide broad-spectrum protection against pathogens.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Yuan-Yuan Zhang, Yu-Hui Hong, Ya-Rong Liu, Jun Cui, Yu-Shi Luan
Summary: miR394 plays a negative role in tomato resistance to late blight, with lncRNA40787 acting as an eTM to regulate LCR. Tthough the expression level of miR394 changes significantly after the infection of various pathogens, our study revealed that overexpression of lncRNA40787 can reduce the expression of miR394 and enhance disease resistance in tomato.
PLANT CELL REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Saptarathi Deb, Maria Cristina Della Lucia, Samathmika Ravi, Giovanni Bertoldo, Piergiorgio Stevanato
Summary: In order to manage late blight in tomato plants, researchers identified disease-associated SNP markers through transcriptome sequencing and validated their effectiveness in 31 tomato varieties with varying disease phenotypes. These markers can be used for molecular screening in tomato breeding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Jose Blanca, Clara Pons, Javier Montero-Pau, David Sanchez-Matarredona, Peio Ziarsolo, Lilian Fontanet, Josef Fisher, Mariola Plazas, Joan Casals, Jose Luis Rambla, Alessandro Riccini, Samuela Pombarella, Alessandra Ruggiero, Maria Sulli, Stephania Grillo, Angelos Kanellis, Giovanni Giuliano, Richard Finkers, Maria Cammareri, Silvana Grandillo, Andrea Mazzucato, Mathilde Causse, Maria Jose Diez, Jaime Prohens, Dani Zamir, Joaquin Canizares, Antonio Jose Monforte, Antonio Granell
Summary: A study on European traditional tomato varieties found that high phenotypic diversity was created by traditional farmer-driven selection, inadvertently combining a few polymorphic loci. Analysis of 1254 tomato accessions revealed a continuous genetic gradient between traditional and modern varieties, with low genetic diversity and distinct genetic groups among traditional European tomatoes. Spanish and Italian varieties showed higher genetic diversity, possibly indicating independent secondary centers of diversity, while other varieties may be the result of recent migrations and hybridizations among European regions. A few highly polymorphic loci were associated with fruit morphological traits in the European traditional collection, suggesting a history of balancing selection by tomato farmers to maintain morphological variation.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Clara Pons, Joan Casals, Samuela Palombieri, Lilian Fontanet, Alessandro Riccini, Jose Luis Rambla, Alessandra Ruggiero, Maria del Rosario Figas, Mariola Plazas, Athanasios Koukounaras, Maurizio E. Picarella, Maria Sulli, Josef Fisher, Peio Ziarsolo, Jose Blanca, Joaquin Canizares, Maria Cammareri, Antonella Vitiello, Giorgia Batelli, Angelos Kanellis, Matthijs Brouwer, Richard Finkers, Konstantinos Nikoloudis, Salvador Soler, Giovanni Giuliano, Stephania Grillo, Silvana Grandillo, Dani Zamir, Andrea Mazzucato, Mathilde Causse, Maria Jose Diez, Jaime Prohens, Antonio Jose Monforte, Antonio Granell
Summary: This study evaluated a large traditional tomato collection from Southern Europe, revealing a wide range of phenotypic variability and differences among countries. By integrating phenotypic data and origin information, eight main tomato types were defined and 159 novel loci associations were identified.
HORTICULTURE RESEARCH
(2022)
Article
Multidisciplinary Sciences
Sven Schrinner, Rebecca Serra Mari, Richard Finkers, Paul Arens, Bjoern Usadel, Tobias Marschall, Gunnar W. Klau
Summary: This article presents a genetic phasing method for plant breeding that can phase the haplotype sequences of parental samples using a large number of low-depth progeny samples. By integrating weak Mendelian progeny signals with a Bayesian log-likelihood model and utilizing an interval scheduling approach, the method can compute high-quality sparse phasings.
Article
Agronomy
Efstathios Roumeliotis, Bjorn Kloosterman, Marian Oortwijn, Wouter Kohlen, Harro J. Bouwmeester, Christian W. B. Bachem, Richard G. F. Visser
Summary: This study investigates the effects of altered auxin content on potato development by cloning and overexpressing the StYUCCA8 gene. The transgenic plants exhibited increased shoot and stolon branching, reduced leaf size, lower average tuber fresh weight, and enhanced adventitious and lateral root formation. Despite the high expression of the StYUCCA8 gene, the concentration of auxin was not altered in the shoot apex but significantly lower in the basal part of the stem.
Review
Genetics & Heredity
Faisal Saeed, Usman Khalid Chaudhry, Ali Raza, Sidra Charagh, Allah Bakhsh, Abhishek Bohra, Sumbul Ali, Annapurna Chitikineni, Yasir Saeed, Richard G. F. Visser, Kadambot H. M. Siddique, Rajeev K. Varshney
Summary: Climate change directly impacts the yield of vegetables through rising temperatures. Breeding for climate-resilient crops requires significant time and effort, but advancements in omics technologies have improved the efficiency of identifying pathways for high-temperature stress resilience in vegetable crops. Genomics-assisted breeding, gene editing, and speed breeding offer new approaches to develop temperature-resilient vegetables more efficiently. This review discusses the effects of heat stress on vegetables and highlights the use of omics and genome editing in producing temperature-resilient vegetables.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Article
Plant Sciences
Chengcheng Cai, Alexandre Pele, Johan Bucher, Richard Finkers, Guusje Bonnema
Summary: Meiotic recombination is crucial for proper chromosome segregation and allelic combination generation. In this study, we analyzed 20 crosses of Brassica oleracea and produced high-resolution recombination maps. The results revealed similar recombination landscapes among different cross combinations and between sexes. We also found that gene density and large structural variations strongly influenced recombination formation in B. oleracea, and there were extensive variations in recombination number depending on the initial parents crossed with.
Article
Agronomy
Corentin R. Clot, Xulan Wang, Joey Koopman, Alejandro Therese Navarro, Johan Bucher, Richard G. F. Visser, Richard Finkers, Herman J. van Eck
Summary: The reinvention of potato as a diploid hybrid crop requires understanding of mutational load, recombination landscape, and genetic basis of fertility. In this study, the OutcrossSeq pipeline was implemented in R for analyzing a large diploid potato population. This pipeline allowed for marker inference, linkage map construction, and QTL mapping, leading to new insights for diploid potato breeding.
Article
Genetics & Heredity
Alejandro Therese Navarro, Peter M. Bourke, Eric van de Weg, Corentin R. Clot, Paul Arens, Richard Finkers, Chris Maliepaard
Summary: Linkage mapping is a method for ordering markers based on recombination events, but genotyping errors are common in high-throughput genotyping data. In this study, an algorithm called Smooth Descent (SD) was developed to improve the accuracy of genetic mapping and correct genotyping errors. SD is able to handle any marker type and autopolyploids, and it performs well in the presence of genotyping errors with error rates between 5% and 20% and non-homogeneous error rates among markers or individuals.
FRONTIERS IN GENETICS
(2023)
Article
Agronomy
Eleni Koseoglou, Matthijs Brouwer, Derek Mudadirwa, Jan M. van der Wolf, Richard G. F. Visser, Yuling Bai
Summary: Research indicates that tolerance to Clavibacter michiganensis (Cm) in tomatoes is controlled by multiple loci, with a major locus located on chromosome 7. However, further investigation reveals that in addition to chromosome 7, there are also other loci on chromosomes 2 and 4 associated with tolerance to Cm.
Article
Plant Sciences
Clara Pons, Joan Casals, Matthijs Brower, Adriana Sacco, Alessandro Riccini, Patrick Hendrickx, Maria del Rosario Figas, Josef Fisher, Silvana Grandillo, Andrea Mazzucato, Salvador Soler, Dani Zamir, Mathilde Causse, Maria Jose Diez, Richard Finkers, Jaime Prohens, Antonio Jose Monforte, Antonio Granell
Summary: European traditional tomato varieties have shown excellent agronomic performance and stability, even outperforming modern varieties. Genome-wide association studies have identified 141 QTLs associated with agro-morphological traits and revealed the genetic basis of stability. This study enhances our understanding of the genetic diversity available in European traditional tomato germplasm.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Multidisciplinary Sciences
Evangelia A. Papoutsoglou, Ioannis N. Athanasiadis, Richard G. F. Visser, Richard Finkers
Summary: Plant phenotyping experiments produce heterogeneous and poorly documented data, making it difficult to reuse. This study explores the challenges and benefits of making data FAIR, using potato developmental traits as a case study. By integrating and verifying datasets with environmental data, we discuss the potential for reproducibility and reusability of FAIR data, along with the challenges encountered in this process.
Article
Genetics & Heredity
Corentin R. Clot, Dennis Klein, Joey Koopman, Cees Schuit, Christel J. M. Engelen, Ronald C. B. Hutten, Matthijs Brouwer, Richard G. F. Visser, Martina Juranic, Herman J. van Eck
Summary: The balanced segregation of homologous chromosomes is crucial for fertility during meiosis. A reduction in crossover (CO) number can lead to unpaired chromosomes and the production of unbalanced, sterile gametes. However, if combined with another meiotic alteration, it can result in uniform, non-recombinant gametes. In potato, a recessive trait known as CO shortage has been mapped to chromosome 8. This study has identified StMSH4 as a candidate gene for CO shortage and discovered nonfunctional alleles in potato cultivars, providing breeders with options for improving fertility or producing uniform gametes.
Article
Plant Sciences
Emily M. S. Ter Steeg, Paul C. Struik, Richard G. F. Visser, Pim Lindhout
Summary: This article analyzes how biological and economic factors determine whether a commercial plant breeder will choose a hybrid breeding system, highlighting the cost of seed production as a key factor. It emphasizes the effectiveness of hybrid breeding in crop genetic improvement, but also points out that high seed production costs are the main hindrance to its commercial application.
Article
Horticulture
Naser Askari, Sasan Aliniaeifard, Richard G. F. Visser
Summary: CO2 starvation in tissue culture has a negative impact on the growth of regenerated plantlets, with or without sucrose in the medium, indicating that reduced CO2 concentrations in vitro result in disrupted photosynthesis functionality and the generation of reactive oxygen species.