Article
Multidisciplinary Sciences
Hua Kui, Bo Ran, Maosen Yang, Xin Shi, Yingyu Luo, Yujie Wang, Tao Wang, Diyan Li, Surong Shuai, Mingzhou Li
Summary: Chickens are important sources of protein for human diet and are used in biomedical research. The skeletal muscles of chickens have diverse origins, shapes, metabolic features, and physical functions, making increasing meat production a key goal in the poultry industry. Previous gene expression studies have overlooked the differences among various types of skeletal muscles. In this study, the researchers sequenced 58 samples from 10 different skeletal muscles of White Plymouth Rock chickens and generated a comprehensive transcriptional map, providing valuable information on muscle fiber characteristics.
Article
Environmental Sciences
Jun Zhu, Yi Zhang, Yawen Xu, Li Wang, Qian Wu, Zhan Zhang, Lei Li
Summary: It has been found in this study that microplastics (MPs) can absorb methylmercury (MeHg) and enhance its accumulation in zebrafish, resulting in reduced locomotor activity and downregulation of neurotransmitter-related genes. This disrupts the health of zebrafish by excessive oxidative stress. This study provides scientific evidence for improving health risk assessment of environmental pollutants.
MARINE ENVIRONMENTAL RESEARCH
(2022)
Article
Genetics & Heredity
Xiangqi Zhong, Jiaze Gu, Siying Zhang, Xiangning Chen, Jingjing Zhang, Jintao Miao, Zhujin Ding, Jianhe Xu, Hanliang Cheng
Summary: In this study, a diet-induced obesity model was developed in zebrafish to investigate the impact of peripheral 5-HT on muscle tissue transcription characteristics. The results demonstrated that peripheral 5-HT affected body weight and gene expression in metabolic pathways related to muscle metabolism in zebrafish.
Article
Environmental Sciences
Ruihong Wang, Shaojuan Guo, Bolun Kang, Lixin Yang
Summary: Methylmercury significantly affected gene expression in zebrafish embryos, particularly the genes involved in visual and sensory perception, phototransduction, ferroptosis, and GABAergic synapse. It also disrupted the expression of genes involved in metabolism, such as fatty acid, amino acid, and glutathione metabolism. Furthermore, genes associated with important developmental signaling pathways like Wnt, Shh, and Notch pathways were also affected. We identified 21 genes that were significantly altered by MeHg independent of concentration and stage, and these genes may serve as signatures for developmental toxicity and/or teratogenic effects.
Article
Genetics & Heredity
Sarah I. Alto, Chih-Ning Chang, Kevin Brown, Chrissa Kioussi, Theresa M. Filtz
Summary: Next-generation sequencing was utilized to analyze gene expression patterns in the soleus and tibialis anterior skeletal muscles, revealing differences in lipid metabolism, glucose metabolism, and muscle contraction. These genetic distinctions explain skeletal muscle specialization and susceptibility to disease and drugs.
Article
Cell Biology
Monika Imierska, Piotr Zabielski, Kamila Roszczyc-Owsiejczuk, Emilia Sokolowska, Karolina Pogodzinska, Iwona Kojta, Agnieszka Blachnio-Zabielska
Summary: Skeletal muscles play a crucial role in insulin-stimulated glucose uptake, and a high-fat diet can lead to metabolic changes in muscles, including the development of insulin resistance. Accumulation of biologically active lipids, particularly ceramides, in muscles is shown to be important in the development of insulin resistance. Silencing the gene responsible for ceramide synthesis can reduce ceramide accumulation and improve the insulin signaling pathway.
Article
Biology
Zherui Xiong, Harriet P. Lo, Kerrie-Ann Mcmahon, Nick Martel, Alun Jones, Michelle M. Hill, Robert G. Parton, Thomas E. Hall
Summary: This method utilizes proximity-dependent biotin labelling in zebrafish to conduct tissue-specific proteomic profiling, demonstrating its applicability in diverse cell types. Using this system, specific interaction networks within muscle cells for closely related Cavin4 and Cavin1 proteins were successfully defined.
Article
Biochemistry & Molecular Biology
Paola De Sanctis, Giuseppe Filardo, Provvidenza Maria Abruzzo, Annalisa Astolfi, Alessandra Bolotta, Valentina Indio, Alessandro Di Martino, Christian Hofer, Helmut Kern, Stefan Loefler, Maurilio Marcacci, Marina Marini, Sandra Zampieri, Cinzia Zucchini
Summary: The study found that exercise training helped skeletal muscle counteract age-related sarcopenia by inducing a wide range of adaptations, sustained by the expression of protein-coding genes. Non-coding RNAs also play a crucial role in orchestrating gene expression during this process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Niclas E. Bengtsson, Hichem Tasfaout, Stephen D. Hauschka, Jeffrey S. Chamberlain
Summary: This study examines the persistence of gene-edited dystrophin expression in different types of muscles in DMD patients. The results indicate that stable dystrophin expression in skeletal muscles will require highly efficient gene editing or cotreatments that decrease skeletal muscle degeneration.
Article
Biochemistry & Molecular Biology
Fuka Takagi, Shozo Tomonaga, Masayuki Funaba, Tohru Matsui
Summary: This study investigated the effects of diets with varying magnesium (Mg) content on the metabolism of rat kidneys and skeletal muscles. The findings suggest that Mg restriction in the diet can lead to alterations in the levels of various metabolites in the kidneys and muscles, potentially resulting in metabolic disorders.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Sanjeeva Metikala, Satish Casie Chetty, Saulius Sumanas
Summary: In this study, single-cell RNA-seq was used to analyze over 20,000 cells disaggregated from the trunk region of zebrafish embryos at the 30 hpf stage, identifying and annotating transcriptional signatures of 27 different cell types and subtypes. This dataset will serve as a valuable resource for researchers in developmental and cellular biology, aiding in the understanding of molecular mechanisms that regulate cell lineage choices during development.
Article
Fisheries
Yu-Cheng Qian, Xue Wang, Jiong Ren, Jie Wang, Samwel Mchele Limbu, Rui-Xin Li, Wen-Hao Zhou, Fang Qiao, Mei-Ling Zhang, Zhen-Yu Du
Summary: The study found that adding 50 mg/kg or 200 mg/kg of TP can improve the adverse effects of high-fat diets on tilapia, reducing hepatic lipid deposition and increasing fish survival rates.
Review
Biochemistry & Molecular Biology
Takuya Kobayashi, Nagomi Kurebayashi, Takashi Murayama
Summary: The Ryanodine receptor (RyR) is a crucial Ca2+ release channel in skeletal and cardiac muscles, regulated by various intracellular factors. Changes in conditions such as exercise, fatigue, and disease states can alter RyR channel activity, indicating its potential role as a sensor for intracellular environment changes in muscles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Alice Printzi, George Koumoundouros, Vincent Fournier, Lauriane Madec, Jose-Luis Zambonino-Infante, David Mazurais
Summary: This study examined the effects of small protein fractions on fish larval and post-larval skeleton. The results showed that 12% shrimp di- and tripeptides were beneficial for growth, survival, and early skeletal quality. They also increased the musculoskeletal resistance of post-larval skeleton. However, the inclusion of live food overshadowed the effects of peptides on fish performance.
Article
Nutrition & Dietetics
Jiaqi Cui, Lin Song, Rui Wang, Shuyuan Hu, Zhao Yang, Zengtie Zhang, Bo Sun, Wei Cui
Summary: This study suggests that maternal metformin during gestation and lactation has the potential to overcome the negative effects of perinatal exposure to a high-fat diet in offspring. This is achieved by altering myogenesis, mitochondrial biogenesis, and dynamics through the AMPK/mTOR pathways in skeletal muscle.
Article
Environmental Sciences
Huong Mai, Jerome Cachot, Christelle Clerandeau, Caroline Martin, Nicolas Mazzela, Patrice Gonzalez, Benedicte Morin
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2020)
Article
Chemistry, Multidisciplinary
Laurie Laloux, Donika Kastrati, Sebastien Cambier, Arno C. Gutleb, Yves-Jacques Schneider
Article
Chemistry, Multidisciplinary
Opeoluwa Ogunsuyi, Olusegun Ogunsuyi, Olubukola Akanni, Okunola Alabi, Chibuisi Alimba, Oluwatosin Adaramoye, Sebastien Cambier, Santhana Eswara, Arno C. Gutleb, Adekunle Bakare
Summary: The study investigated the in vivo toxic effect of a mixture of TiO(2)NPs and ZnO NPs, revealing that the mixture induced a higher systemic toxicity than when TiO(2)NPs or ZnO NPs were used individually. Comprehensive risk assessments should be carried out on NP mixtures before their utilization in consumer products.
DRUG AND CHEMICAL TOXICOLOGY
(2022)
Article
Environmental Sciences
Eliska Kuchovska, Benedicte Morin, Rocio Lopez-Cabeza, Mathilde Barre, Corentin Gouffier, Lucie Blahova, Jerome Cachot, Ludek Blaha, Patrice Gonzalez
Summary: This study investigated the impacts of commonly detected insecticide and fungicide on the early development of Pacific oysters in the Arcachon Bay, France. Results suggest that current pesticide concentrations are safe for oyster early development but may have small direct effects through altered gene expressions.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Review
Environmental Sciences
Amalia Rosner, Jean Armengaud, Loriano Ballarin, Stephanie Barnay-Verdier, Francesca Cima, Ana Varela Coelho, Isabelle Domart-Coulon, Damjana Drobne, Anne-Marie Geneviere, Anita Jemec Kokalj, Ewa Kotlarska, Daniel Mark Lyons, Tali Mass, Guy Paz, Ksenia Pazdro, Lorena Peric, Andreja Ramsak, Sebastian Rakers, Baruch Rinkevich, Antonietta Spagnuolo, Michela Sugni, Sebastien Cambier
Summary: Assessment of environmental stressors involves quantifying their impacts on various indicators such as species density, growth rates, reproduction, behavior, and physiology, as well as host-pathogen interactions and immunocompetence. Environmental stress may induce sublethal effects like mutations and epigenetic signatures, affecting offspring via transgenerational inheritance.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Kahina Mehennaoui, Sebastien Cambier, Laetitia Minguez, Tommaso Serchi, Francois Guerold, Arno C. Gutleb, Laure Giamberini
Summary: The study aimed to evaluate the sub-chronic effects of silver (AgNPs) and gold nanoparticles (AuNPs) of 40 nm on the freshwater invertebrate Gammarus fossarum. The exposure led to significant uptake of Ag and Au in the animals but below toxic levels, while causing up-regulation of specific gene expressions and a more developed lysosomal system in response to stress. The nanoparticles also significantly affected locomotor activity of G. fossarum without impacting haemolymphatic ions and ventilation.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Environmental Sciences
Kevin Gillois, Charlotte Stoffels, Mathilde Leveque, Isabelle Fourquaux, Justine Blesson, Valerie Mils, Sebastien Cambier, Julien Vignard, Helene Terrisse, Gladys Mirey, Jean-Nicolas Audinot, Vassilia Theodorou, Marie-Helene Ropers, Herve Robert, Muriel Mercier-Bonin
Summary: The study found that two colloidal silver products, Mesosilver and AgC, exhibit different levels of gut toxicity due to the distinct forms of silver they contain. AgC, composed solely of ionic silver, showed more toxic effects on cells compared to Mesosilver, which contains a mix of AgNPs and ionic silver. The presence of mucus in the co-culture model slightly mitigated the toxic effects on cell viability.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Multidisciplinary Sciences
Syed Shaheen Shah, Mohammed Ameen Ahmed Qasem, Roberto Berni, Cecilia Del Casino, Giampiero Cai, Servane Contal, Irshad Ahmad, Khawar Sohail Siddiqui, Edoardo Gatti, Stefano Predieri, Jean-Francois Hausman, Sebastien Cambier, Gea Guerriero, Md Abdul Aziz
Summary: Nettle can be used for the preparation of carbon nanosheets, offering advantages such as fast growth and homogenous material propagation. Research shows that leaf carbon nanosheets have no inhibitory effects on pollen germination, but inhibit algal growth at higher concentrations.
SCIENTIFIC REPORTS
(2021)
Article
Environmental Sciences
Laure Laffont, Johanna Menges, Sylvaine Goix, Sophie Gentes, Regine Maury-Brachet, Jeroen E. Sonke, Alexia Legeay, Patrice Gonzalez, Raphaelle Rinaldo, Laurence Maurice
Summary: The study in French Guiana aimed to investigate the impact of gold-mining activities on mercury concentrations and isotopic signatures in freshwater fish consumed by local residents. Analysis of Hg concentrations and isotopic ratios in fish muscles revealed differences in biogeochemical processes between fish from impacted and pristine areas, indicating potential influences of suspended particles on mercury concentrations. The study also highlighted the potential of Hg isotopes in fish as complementary tracers to understand the sources and processes of mercury accumulation in aquatic ecosystems.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Environmental Sciences
Nhung H. A. Nguyen, Yehia S. El-Temsah, Sebastien Cambier, Magdalena Calusinska, Pavel Hrabak, Miloslav Pouzar, Martin Boruvka, Pavel Kejzlar, Totka Bakalova, Arno C. Gutleb, Alena Sevcu
Summary: This study compared the impacts of different bio-based plastics on natural bacterial communities in seawater and freshwater, revealing significant differences between microbial communities forming biofilms on different plastics and planktonic bacteria. Different bacterial groups were found to dominate on different bio-based plastics in both marine and freshwater environments.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Analytical
Olivier De Castro, Antje Biesemeier, Eduardo Serralta, Olivier Bouton, Rachid Barrahma, Quang H. Hoang, Sebastien Cambier, Tatjana Taubitz, Nico Klingner, Gregor Hlawacek, Serge Duarte Pinto, Peter Gnauck, Falk Lucas, Cecilia Bebeacua, Tom Wirtz
Summary: Nanoparticles have become essential in various scientific and technological fields, but their characterization usually requires complex workflows. The development of a new in situ correlative instrument has shown to significantly increase efficiency and shorten the duration of studies by combining various microscopic and analytical capabilities. The novel instrument's performance and usefulness in nanoparticle investigations have been demonstrated, opening up possibilities for future applications, particularly in nanotoxicological research.
ANALYTICAL CHEMISTRY
(2021)
Article
Green & Sustainable Science & Technology
Melanie Guiton, David Suarez-Montes, Ruben Sanchez, Paul Baustert, Christos Soukoulis, Burcu Saner Okan, Tommaso Serchi, Sebastien Cambier, Enrico Benetto
Summary: Microalgae has emerged as an alternative resource to terrestrial plant-based biomass. This study presents a comprehensive Life Cycle Assessment (LCA) of microalgae-based Metal Working Fluids (MWF) and compares it with vegetable and mineral alternatives. The results show that significant improvements are needed to make microalgae-based MWF more environmentally friendly compared to its alternatives.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Chemistry, Applied
Thierry Hellebois, Claire Gaiani, Sebastien Cambier, Anais Noo, Christos Soukoulis
Summary: This study investigated the structuring and stabilising potential of flaxseed gum in whey protein isolate cryo-hydrogels. The presence of flaxseed gum induced segregative phase separation in heat-treated WPI dispersions, and cryotropic processing led to the formation of diverse macroporous protein gel networks. Covalent and non-covalent interactions played roles in mediating cryogel formation and stabilisation. Flaxseed gum improved the homogeneity and connectivity of the protein network, reduced its colloidal instability, and macroporosity.
CARBOHYDRATE POLYMERS
(2022)
Article
Toxicology
Aline Chary, Katherine Groff, Andreas O. Stucki, Servane Contal, Charlotte Stoffels, Sebastien Cambier, Monita Sharma, Arno C. Gutleb, Amy J. Clippinger
Summary: The research demonstrated successful transition of A549 cells from FBS-containing to FBS-free media, showing differences in cellular characteristics between the two FBS-free media.
TOXICOLOGY IN VITRO
(2022)
Article
Chemistry, Multidisciplinary
Caterina Minelli, Magdalena Wywijas, Dorota Bartczak, Susana Cuello-Nunez, Heidi Goenaga Infante, Jerome Deumer, Christian Gollwitzer, Michael Krumrey, Karen E. Murphy, Monique E. Johnson, Antonio R. Montoro Bustos, Ingo H. Strenge, Bertrand Faure, Peter Hoghoj, Vivian Tong, Loic Burr, Karin Norling, Fredrik Hook, Matthias Roesslein, Jovana Kocic, Lyndsey Hendriks, Vikram Kestens, Yannic Ramaye, Maria C. Contreras Lopez, Guy Auclair, Dora Mehn, Douglas Gilliland, Annegret Potthoff, Kathrin Oelschlagel, Jutta Tentschert, Harald Jungnickel, Benjamin C. Krause, Yves U. Hachenberger, Philipp Reichardt, Andreas Luch, Thomas E. Whittaker, Molly M. Stevens, Shalini Gupta, Akash Singh, Fang-hsin Lin, Yi-Hung Liu, Anna Luisa Costa, Carlo Baldisserri, Rid Jawad, Samir E. L. Andaloussi, Margaret N. Holme, Tae Geol Lee, Minjeong Kwak, Jaeseok Kim, Johanna Ziebel, Cedric Guignard, Sebastien Cambier, Servane Contal, Arno C. Gutleb, Jan Kuba Tatarkiewicz, Bartlomiej J. Jankiewicz, Bartosz Bartosewicz, Xiaochun Wu, Jeffrey A. Fagan, Elisabeth Elje, Elise Runden-Pran, Maria Dusinska, Inder Preet Kaur, David Price, Ian Nesbitt, Sarah O'Reilly, Ruud J. B. Peters, Guillaume Bucher, Dennis Coleman, Angela J. Harrison, Antoine Ghanem, Anne Gering, Eileen McCarron, Niamh Fitzgerald, Geert Cornelis, Jani Tuoriniemi, Midori Sakai, Hidehisa Tsuchida, Ciaran Maguire, Adriele Prina-Mello, Alan J. Lawlor, Jessica Adams, Carolin L. Schultz, Doru Constantin, Nguyen Thi Kim Thanh, Le Duc Tung, Luca Panariello, Spyridon Damilos, Asterios Gavriilidis, Iseult Lynch, Benjamin Fryer, Ana Carrazco Quevedo, Emily Guggenheim, Sophie Briffa, Eugenia Valsami-Jones, Yuxiong Huang, Arturo A. Keller, Virva-Tuuli Kinnunen, Siiri Peramaki, Zeljka Krpetic, Michael Greenwood, Alexander G. Shard
Summary: This study evaluates the repeatability and reproducibility of different measurement methods for the particle number concentration of colloidal nanoparticles. The results show that population-averaging methods have higher repeatability and reproducibility than particle-counting methods.