Article
Microbiology
Tania Henriquez, Heinrich Jung
Summary: The MxtR/ErdR system is essential for acetate utilization in Pseudomonas putida KT2440, stimulating the expression of specific genes, but does not play a role in regulating siderophore production and the expression of RND transporters.
Article
Microbiology
Chenchen Xu, Qiao Cao, Lefu Lan
Summary: Research shows that the periplasmic binding protein GltB plays a crucial role in activating GtrS-GltR in response to glucose in P. aeruginosa and together they regulate the virulence of P. aeruginosa in response to glucose. Furthermore, the activation of GltB is closely related to the virulence of P. aeruginosa.
Article
Biochemistry & Molecular Biology
Shreya Ghosh, Anam Ejaz, Lucas Repeta, Stewart Shuman
Summary: The study identifies Pseudomonas putida MPE as a novel DNA endonuclease capable of sensing and cutting double-stranded DNA, and hydrolyzing the single-stranded DNA backbone in different orientations to generate distinct cleavage products.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Engineering, Environmental
Xiaoqing Meng, Hua Li, Su Yan, Zengping Ning, Quan Yuan, Chengshuai Liu, Lixiang Zhou, Guanyu Zheng
Summary: The present study found that pre-acidification greatly promoted the inactivation of a multi-drug resistant bacterium and the degradation of carried antibiotic resistance genes (ARGs) in wastewater. Pre-acidification led to cell lysis and the release of ARGs, which were then degraded by the generated radicals. This study highlights the importance of efficiently lysing ARB cells to promote the degradation of carried ARGs and reduce antibiotic resistance in wastewater.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Hyun Gyu Lim, Kevin Rychel, Anand Sastry, Gayle J. Bentley, Joshua Mueller, Heidi S. Schindel, Peter E. Larsen, Philip D. Laible, Adam M. Guss, Wei Niu, Christopher W. Johnson, Gregg T. Beckham, Adam M. Feist, Bernhard O. Palsson
Summary: This study applies a machine-learning approach to uncover the genome-scale transcriptional regulatory network in Pseudomonas putida KT2440, providing a basis for understanding its physiological processes and engineering it for practical use.
METABOLIC ENGINEERING
(2022)
Article
Multidisciplinary Sciences
Joshua R. Elmore, Gara N. Dexter, Davinia Salvachua, Jessica Martinez-Baird, E. Anne Hatmaker, Jay D. Huenemann, Dawn M. Klingeman, George L. Peabody, Darren J. Peterson, Christine Singer, Gregg T. Beckham, Adam M. Guss
Summary: Expanding lignin-derived product portfolio is crucial for sustainable bio-based economy. Researchers engineered Pseudomonas putida to produce itaconic acid at high yields from lignin-derived compounds. Dynamic metabolic control rerouting carbon in central metabolism enabled high yields of valuable chemicals from lignin.
NATURE COMMUNICATIONS
(2021)
Article
Agricultural Engineering
Chunzhe Lu, Nina Leitner, Rene H. Wijffels, Vitor A. P. Martins dos Santos, Ruud A. Weusthuis
Summary: This study developed a biocatalyst for the production of mcl-diols from n-alkanes under mild conditions. The engineered strain P. putida KT2440, with overexpression of Est12, can efficiently hydrolyze esterified diols, enabling the production of mcl-diols.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Truus de Vrije, Ricardo M. Nagtegaal, Ruud M. Veloo, Frans H. J. Kappen, Frits A. de Wolf
Summary: By feeding ethanol to Pseudomonas putida growing in liquid obtained from acidogenic digestion of organic municipal solid waste, production of medium chain length polyhydroxyalkanoate (mcl-PHA) up to about 6 g.L-1 was achieved. Washing the wet, heat-inactivated Pseudomonas cells with ethanol at the end of the fermentation eliminated the need for drying the biomass and allowed the removal of contaminating lipids before solvent-mediated extraction of PHA. 'Green' solvents were used to extract 90 to near 100% of the mcl-PHA, and purities of 71-78% were obtained by centrifugation and decantation without further filtration for biomass removal.
BIORESOURCE TECHNOLOGY
(2023)
Article
Food Science & Technology
Lin Wang, Xueli Cao, Hairun Pei, Ping Liu, Ya Song, Yulun Wu
Summary: Chlorogenic acid has an anti-biofilm effect on drinking water bacteria, attenuates biofilm structure, and affects metabolic pathways. It may act as a quorum sensing inhibitor and has the potential to prevent contaminated drinking water.
Article
Agricultural Engineering
Jin Li, Cheng Yue, Wenping Wei, Yanzhe Shang, Ping Zhang, Bang-Ce Ye
Summary: This study successfully developed an auto-regulatory system to address the challenging issues in lignin valorization, demonstrating its effectiveness and economic advantages.
BIORESOURCE TECHNOLOGY
(2022)
Article
Microbiology
Di-Yin Li, Jian-Ting Han, Meng-Yuan Zhang, Xu Yan, Nannan Zhang, Honghua Ge, Zhiping Wang, Yong-Xing He
Summary: The two-component system RstA/RstB regulates the expression of multidrug resistance efflux pumps and enzymes involved in anaerobic nitrate respiration and pyoverdine biosynthesis, enhancing bacterial tolerance to antibiotics and hypoxic environments.
Article
Microbiology
Ruolin Qin, Tao Xu, Xiaoqiang Jia
Summary: Phenanthrene biodegradation is often limited by its low water solubility and dissolution rate. In this study, we engineered a strain of Pseudomonas putida to produce rhamnolipid biosurfactants, which promote the biodegradation of phenanthrene by an engineered strain of Escherichia coli. The two-species consortium exhibited a synergistic effect in degrading phenanthrene, resulting in a significant increase in the degradation ratio.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Multidisciplinary
Jorge Arreola-Vargas, Xianzhi Meng, Yun-yan Wang, Arthur J. Ragauskas, Joshua S. Yuan
Summary: The conversion of lignin to mcl-PHA presents a promising alternative to petroleum-based plastics, particularly when combined with treated lignocellulosic biomass. Optimal fermentation conditions and the combination of lignin stream with enzymatic hydrolysate can significantly increase mcl-PHA production, highlighting the potential of utilizing different fractions of lignocellulosic biomass for bioplastic production.
Article
Microbiology
Tania Henriquez, Jyh-Shiuan Hsu, Jakob Sebastian Hernandez, Sonja Kuppermann, Michelle Eder, Heinrich Jung
Summary: This study investigates the function and physiological significance of the MxtR/ErdR two-component system and its target genes in Pseudomonas putida KT2440. The findings suggest that the target gene scpC is important for the bacterium to cope with high concentrations of acetate, while pp_0353 and pp_0354 are not essential for growth on acetate. Additionally, MxtR/ErdR is involved in the metabolism of pyruvate. These findings expand our understanding of the role of the MxtR/ErdR two-component system in carbon source utilization.
MICROBIOLOGY SPECTRUM
(2023)
Article
Virology
Guanhua Xuan, Hong Lin, Xiaoyan Li, Jiuna Kong, Jingxue Wang
Summary: Our study reported that the complex multisensing regulatory networks RetS-GacS/GacA of Pseudomonas aeruginosa PAO1 play key roles in controlling phage infection. The main observation was that the mutation in RetS could result in increased phage resistance by reducing the type IV pilus-mediated phage adsorption. The bacterial defense strategy is generally applicable to various phages since many P. aeruginosa phages can use type IV pilus as their receptors. The results also suggest that the phage infection is likely to be regulated dynamically, which depends on the environmental stimuli. Reduction of the signals that RetS favors would increase phage resistance. Our study is particularly remarkable for uncovering a signal transduction system that was involved in phage infection, which may help in filling some knowledge gaps in this field.
JOURNAL OF VIROLOGY
(2022)
Review
Genetics & Heredity
Rita Horak, Hedvig Tamman
Article
Medicine, Research & Experimental
Liis Preem, Mohammad Mahmoudzadeh, Marta Putrins, Andres Meos, Ivo Laidmae, Tavo Romann, Jaan Aruvali, Riinu Harmas, Artturi Koivuniemi, Alex Bunker, Tanel Tenson, Karin Kogermann
MOLECULAR PHARMACEUTICS
(2017)
Article
Biochemical Research Methods
Ariel Talavera, Hedvig Tamman, Andres Ainelo, San Hadzi, Abel Garcia-Pino, Rita Horak, Albert Konijnenberg, Remy Loris
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2017)
Article
Pharmacology & Pharmacy
Spela Zupancic, Liis Preem, Julijana Kristl, Marta Putrins, Tanel Tenson, Petra Kocbek, Karin Kogermann
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2018)
Article
Microbiology
Frederic Goormaghtigh, Nathan Fraikin, Marta Putrins, Thibaut Hallaert, Vasili Hauryliuk, Abel Garcia-Pino, Andreas Sjodin, Sergo Kasvandik, Klas Udekwu, Tanel Tenson, Niilo Kaldalu, Laurence Van Melderen
Letter
Microbiology
Frederic Goormaghtigh, Nathan Fraikin, Marta Putrins, Vasili Hauryliuk, Abel Garcia-Pino, Klas Udekwu, Tanel Tenson, Niilo Kaldalu, Laurence Van Melderen
Article
Biochemistry & Molecular Biology
Victoriia Murinat, Marje Kasari, Hiraku Takada, Mariliis Hinnu, Chayan Kumar Saha, James W. Grimshaw, Takahiro Seki, Michael Reith, Marta Putrins, Tanel Tenson, Henrik Strahl, Vasili Hauryliuk, Gemma Catherine Atkinson
JOURNAL OF MOLECULAR BIOLOGY
(2019)
Article
Food Science & Technology
Andres Ainelo, Rando Porosk, Kalle Kilk, Sirli Rosendahl, Jaanus Remme, Rita Horak
Article
Microbiology
Kadi Ainsaar, Hedvig Tamman, Sergo Kasvandik, Tanel Tenson, Rita Horak
JOURNAL OF BACTERIOLOGY
(2019)
Article
Genetics & Heredity
Katren Mikkel, Mari Tagel, Kart Ukkivi, Heili Ilves, Maia Kivisaar
Article
Microbiology
Mari Tagel, Heili Ilves, Margus Leppik, Karl Jurgenstein, Jaanus Remme, Maia Kivisaar
Summary: The study demonstrates a link between tRNA pseudouridylation and mutation frequency in Pseudomonas species, showing that deficiency in TruA-dependent pseudouridylation increases mutation frequency. This mutagenic effect is not attributed to error-prone DNA polymerases or malfunctioning of DNA repair pathways. It suggests that the elevated mutation frequency in the absence of tRNA pseudouridylation could be due to a more specific response or a cumulative effect of several small effects.
Article
Microbiology
Sirli Rosendahl, Andres Ainelo, Rita Horak
Summary: The translation explains the importance of chaperone protein DnaK in interacting with the toxin GraT and modulating its activity, particularly through the negatively charged motif in the C-terminus. This interaction is crucial for P. putida's competitive fitness at different temperatures.
Article
Microbiology
Ivana Kerkez, Paul M. Tulkens, Tanel Tenson, Francoise Van Bambeke, Marta Putrins
Summary: Research shows that commonly used antibiotics for urinary tract infections are much less effective against intracellular UPEC compared to extracellular UPEC. Except fluoroquinolones, most antibiotics were unable to achieve a bactericidal effect intracellularly at clinically achievable concentrations. Fluoroquinolones ciprofloxacin and finafloxacin killed almost all extracellular bacteria at concentrations close to the MIC, but significantly higher concentrations were needed for intracellular bacteria.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Karl Jurgenstein, Mari Tagel, Heili Ilves, Margus Leppik, Maia Kivisaar, Jaanus Remme
Summary: Variations in the translational machinery affect protein synthesis efficiency in different organisms. The study shows that species more prone to translational frameshifting are not necessarily more prone to stop codon readthrough. The presence of pseudouridines in the anticodon stem-loop of tRNA influences translational fidelity in a context-specific manner, but this effect differs between species.
Review
Microbiology
Niilo Kaldalu, Vasili Hauryliuk, Kathryn Jane Turnbull, Agnese La Mensa, Marta Putrin, Tanel Tenson
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2020)