Article
Chemistry, Applied
Alejandra Aragon-Leon, Lorena Moreno-Vilet, Marisela Gonzalez-Avila, Pedro Martin Mondragon-Cortez, Guilherme Lanzi Sassaki, Raul Balam Martinez-Perez, Rosa Maria Camacho-Ruiz
Summary: This study evaluated the production, chemical characterization, biological and technological properties of inulin biosynthesized by a halophilic archaeon. The inulin showed high production, emulsifying index, and prebiotic capacity.
CARBOHYDRATE POLYMERS
(2023)
Article
Biochemistry & Molecular Biology
Jing Hou, Xue-Meng Yin, Yang Li, Dong Han, Bu Lu, Jia-Yi Zhang, Heng-Lin Cui
Summary: The extracellular protease HlyA from Halococcus salifodinae exhibited good temperature, pH, and salinity tolerance, with significant enhancements in activity by Ca2+, Mg2+, and Sr2+. It was completely inhibited by phenylmethanesulfonyl fluoride (PMSF), indicating it is a serine protease. Additionally, HlyA showed good tolerance to some surfactants and organic solvents, making it a promising candidate for industrial applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Food Science & Technology
Wei Wei, Xinyu Hu, Sai Yang, Kaili Wang, Chunlin Zeng, Zhaozhi Hou, Henglin Cui, Shenkui Liu, Lin Zhu
Summary: This study identified the microbial groups responsible for the natural degradation of nitrite in salted radish and elucidated the associated metabolic mechanism. Halophilic archaea from pickling salt dominate the denitrification pathway, with key genera including Haloarcula, Halolamina, and Halobacterium. These archaea cope with high salt stress through a salt-in mechanism and efficiently reduce nitrite to nitrogen, providing important insights for nitrite hazard prevention in high-salinity salted vegetables.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biotechnology & Applied Microbiology
Magdalena Kowalewicz-Kulbat, Krzysztof T. Krawczyk, Izabela Szulc-Kielbik, Sebastian Rykowski, Marta Denel-Bobrowska, Agnieszka B. Olejniczak, Camille Locht, Magdalena Klink
Summary: Halorhabdus rudnickae and Natrinema salaciae, isolated from a Polish salt mine and Lake Medee in the Mediterranean Sea, secrete metabolites that are active against ovarian cancer cells, including those that are resistant to cisplatin, suggesting potential new possibilities for the treatment of these gynaecological cancers.
MICROBIAL CELL FACTORIES
(2023)
Article
Biotechnology & Applied Microbiology
Jing Hou, Si-Ya Li, Yang-Jie Zhao, Heng-Lin Cui
Summary: This study identified a novel type of halolysin (Hly(Hap)) without C-terminal extension (CTE) from the extremely halophilic archaeon Haladaptatus sp. DYF46. Hly(Hap) exhibited stability and optimal activity in a low salinity condition despite being derived from an extremely halophilic archaeon. It was also found that Ca2+ and Mg2+ significantly promoted the activity of Hly(Hap). Moreover, Hly(Hap) showed stability in the presence of organic solvents and detergents.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Komal Ghauri, Tjaard Pijning, Nayla Munawar, Hazrat Ali, Muhammad A. Ghauri, Munir A. Anwar, Russell Wallis
Summary: The crystal structure of an inulin-type fructan producing enzyme from the archaeon Halalkalicoccus jeotgali was resolved in this study, revealing unique features compared to previously reported FTFs. The high negative electrostatic surface potential of InuHj likely contributes to its halophilicity. The findings provide targets for future structure-function studies of FTF enzymes, especially those from archaea.
Article
Genetics & Heredity
Clifton P. Bueno de Mesquita, Jinglie Zhou, Susanna M. Theroux, Susannah G. Tringe
Summary: Anaerobic archaeal methanogens, specifically an unclassified Methanosarcinaceae methanogen named Methanosalis, play a key role in the global carbon cycle by decomposing organic matter in hypersaline environments. The genome of Methanosalis, isolated from sediments of an industrial saltern, contains pathways for methylotrophic methanogenesis and transport of compatible solutes, indicating its importance as a methane source. This MAG was more abundant in industrial salterns with high salinity levels compared to nearby wetlands with lower methane emissions.
Article
Biochemistry & Molecular Biology
Mar Martinez Pastor, Saaz Sakrikar, Deyra N. Rodriguez, Amy K. Schmid
Summary: Due to the lack of commercial and specifically designed rRNA depletion kits, the study of genome-scale gene expression in archaea has faced challenges. Different hybridization-based kits from different suppliers have varying efficiency in removing rRNA from archaea. The selection of appropriate reagents and strategies is crucial for the success of transcriptomic research in archaea.
Article
Biochemistry & Molecular Biology
Roberto Marin-Paredes, Hermes H. Bolivar-Torres, Alberto Coronel-Gaytan, Esperanza Martinez-Romero, Luis E. Servin-Garciduenas
Summary: This study sequenced a metagenome from a sediment sample from Los Azufres National Park and identified a diverse microbial community consisting of bacteria, archaea, eukarya, and viruses. The functional annotation of the metagenome revealed genes related to various important biological processes. Two metagenome-assembled genomes from Ferrimicrobium and Cuniculiplasma were also recovered from the sediment sample.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Microbiology
Zhichao Zhou, Emily St. John, Karthik Anantharaman, Anna-Louise Reysenbach
Summary: This study explores the genomic diversity in 42 metagenomes from deep-sea hydrothermal vents and documents their potential implications in biogeochemical cycles. The findings reveal a significant expansion of microbial genomic diversity associated with hydrothermal vent deposits and highlight the metabolic adaptation of taxonomic guilds.
Article
Microbiology
Galina Slobodkina, Maxime Allioux, Alexander Merkel, Marie-Anne Cambon-Bonavita, Karine Alain, Mohamed Jebbar, Alexander Slobodkin
Summary: A new species of Archaeoglobus, Archaeoglobus neptunius, has been isolated and characterized from a deep-sea hydrothermal vent. The novel isolate exhibits unique growth conditions and metabolic features, suggesting potential biotechnological applications. Genomic analysis supports the reclassification of species within the genus Archaeoglobus.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Xianhui S. Wan, Lei Hou, Shuh-Ji Kao, Yao Zhang, Hua-Xia Sheng, Hui Shen, Senwei Tong, Wei Qin, Bess B. Ward
Summary: The ocean is a net source of nitrous oxide, a greenhouse gas and ozone-depleting substance. It is primarily produced during ammonia oxidation by dominant marine archaea. The pathways and kinetics of nitrous oxide production are not fully understood.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Microbiology
Ana Sofia Vazquez-Madrigal, Alejandra Barbachano-Torres, Melchor Arellano-Plaza, Manuel Reinhart Kirchmayr, Ilaria Finore, Annarita Poli, Barbara Nicolaus, Susana De la Torre Zavala, Rosa Maria Camacho-Ruiz
Summary: This study identified seventeen halophilic archaea from the Santa Barbara saltern in Sonora, Mexico, belonging to Haloarcula, Halolamina, and Halorubrum genera. The presence of neutral lipids and different phospholipids in these strains was confirmed. Carotenoid production in response to glucose, succinic acid, and glutamic acid varied among the strains, with Halorubrum sp. M5 showing negative effects from certain carbon sources. This work highlights the diversity in growth and carotenoid production of haloarchaea under different carbon sources.
Article
Microbiology
Hye Seon Song, Juseok Kim, Yeon Bee Kim, Se Hee Lee, Tae Woong Whon, Seong Woon Roh
Summary: Two novel halophilic archaeal strains were isolated from solar salt in South Korea. Through genetic, physiological, and biochemical analyses, they were found to represent a new genus and species in the family Halobacteriaceae.
JOURNAL OF MICROBIOLOGY
(2022)
Article
Environmental Sciences
Bing-Bing Liu, Rajivgandhi Govindan, Maruthupandy Muthuchamy, Shuang Cheng, Xuebin Li, Lijing Ye, Lai-you Wang, Shu-xian Guo, Wen-Jun Li, Naiyf S. Alharbi, Jamal M. Khaled, Shine Kadaikunnan
Summary: In this study, 75 halophilic archaeal strains were isolated from Wuyongbulake salt lake sediment sample using phenol enrichment method. The majority of the identified strains were phenol-tolerant. The suspension of strains 869-1 and 121-1 exhibited the highest phenol degradation rate, while the supernatant of strain 630-3 showed the highest rate in a simulated artificial wastewater experiment. The EPS produced by strains A00135, 558-1, 869-1, 121-1, and A387 showed excellent phenol removal ability.