Article
Biotechnology & Applied Microbiology
Jing Hou, Si-Ya Li, Yang-Jie Zhao, Heng-Lin Cui
Summary: This study identified a novel type of halolysin (Hly(Hap)) without C-terminal extension (CTE) from the extremely halophilic archaeon Haladaptatus sp. DYF46. Hly(Hap) exhibited stability and optimal activity in a low salinity condition despite being derived from an extremely halophilic archaeon. It was also found that Ca2+ and Mg2+ significantly promoted the activity of Hly(Hap). Moreover, Hly(Hap) showed stability in the presence of organic solvents and detergents.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Applied
Alejandra Aragon-Leon, Lorena Moreno-Vilet, Marisela Gonzalez-Avila, Pedro Martin Mondragon-Cortez, Guilherme Lanzi Sassaki, Raul Balam Martinez-Perez, Rosa Maria Camacho-Ruiz
Summary: This study evaluated the production, chemical characterization, biological and technological properties of inulin biosynthesized by a halophilic archaeon. The inulin showed high production, emulsifying index, and prebiotic capacity.
CARBOHYDRATE POLYMERS
(2023)
Article
Environmental Sciences
Bing-Bing Liu, Rajivgandhi Govindan, Maruthupandy Muthuchamy, Shuang Cheng, Xuebin Li, Lijing Ye, Lai-you Wang, Shu-xian Guo, Wen-Jun Li, Naiyf S. Alharbi, Jamal M. Khaled, Shine Kadaikunnan
Summary: In this study, 75 halophilic archaeal strains were isolated from Wuyongbulake salt lake sediment sample using phenol enrichment method. The majority of the identified strains were phenol-tolerant. The suspension of strains 869-1 and 121-1 exhibited the highest phenol degradation rate, while the supernatant of strain 630-3 showed the highest rate in a simulated artificial wastewater experiment. The EPS produced by strains A00135, 558-1, 869-1, 121-1, and A387 showed excellent phenol removal ability.
Article
Biotechnology & Applied Microbiology
Magdalena Kowalewicz-Kulbat, Krzysztof T. Krawczyk, Izabela Szulc-Kielbik, Sebastian Rykowski, Marta Denel-Bobrowska, Agnieszka B. Olejniczak, Camille Locht, Magdalena Klink
Summary: Halorhabdus rudnickae and Natrinema salaciae, isolated from a Polish salt mine and Lake Medee in the Mediterranean Sea, secrete metabolites that are active against ovarian cancer cells, including those that are resistant to cisplatin, suggesting potential new possibilities for the treatment of these gynaecological cancers.
MICROBIAL CELL FACTORIES
(2023)
Article
Biochemistry & Molecular Biology
Hoang Thi Hong Anh, Esmaeil Shahsavari, Nathan J. Bott, Andrew S. Ball
Summary: This study investigated the co-culture of two halophilic bacteria, Marinirhabdus sp. and Marinobacter hydrocarbonoclasticus, and found that the co-culture resulted in increased protease activity and biomass production. Importantly, the protease activity in the co-culture remained active over a broader range of environmental conditions, making it an economical and practical approach for enhanced biotechnological applications.
Article
Multidisciplinary Sciences
Ibrahim Ahmed Shaikh, Bhagya Turakani, Jay Malpani, Susmita Goudar, Mater H. Mahnashi, Rasha Hamed Al-Serwi, Mohammed M. Ghoneim, Mohamed El-Sherbiny, Basheerahmed Abdulaziz Mannasaheb, Fahad Alsaikhan, Vaishani Sindagimath, Aejaz Abdullatif Khan, Uday M. Muddapur, Solafa Azzouz, Tasneem Mohammed, S. M. Shakeel Iqubal
Summary: The study aimed to isolate bacteria that synthesize protease enzymes from a soil sample in Dandeli, Karnataka, India. Screening, production, and optimization of medium components were conducted to maximize protease activity. Partial purification of crude enzymes and application of protease produced by Bacillus nakamurai were carried out. The optimized parameters for protease production can enhance its application in various industries.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Fabian Falkenberg, Leonie Voss, Michael Bott, Johannes Bongaerts, Petra Siegert
Summary: The aim of this study was to characterize four new subtilisins from halotolerant and halophilic microorganisms. The proteases showed activity over a wide pH range and exhibited high stability towards SDS and high concentrations of NaCl. These enzymes demonstrate potential for future biotechnological applications.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mar Martinez Pastor, Saaz Sakrikar, Deyra N. Rodriguez, Amy K. Schmid
Summary: Due to the lack of commercial and specifically designed rRNA depletion kits, the study of genome-scale gene expression in archaea has faced challenges. Different hybridization-based kits from different suppliers have varying efficiency in removing rRNA from archaea. The selection of appropriate reagents and strategies is crucial for the success of transcriptomic research in archaea.
Article
Biotechnology & Applied Microbiology
Shenao Zhang, Feilong Chen, Juntao Ke, Yuling Hao, Ruru Pan, Tao Hong, Yongpei Dai, Shaoxing Chen
Summary: Extracellular proteases of haloarchaea have the ability to adapt to high salt concentrations and can be utilized in industrial or biotechnology applications under hypersaline conditions. However, the diversity of these proteases is largely unknown despite the availability of many sequenced genomes. In this study, the gene encoding the extracellular protease Hly176B from Haloarchaeobius sp. FL176 was cloned and expressed in Escherichia coli, revealing its catalytic triad and unique properties. This research enhances our understanding of extracellular proteases and broadens their potential industrial uses.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Essam Kotb, Baher A. El-Nogoumy, Haifa A. Alqahtani, Asmaa A. Ahmed, Hussah A. Al-shwyeh, Sakina M. Algarudi, Hanan Almahasheer
Summary: A putative virulence exoprotease UcB5 was purified from Salmonella typhimurium with high purity and recovery. It exhibited serine protease-type characteristics and had broad substrate specificity. UcB5 was found to cause subcellular proteolysis and liver necrosis, suggesting its potential role in microbial diseases. Future research should focus on combination therapy using external antiproteases and antimicrobial agents.
SCIENTIFIC REPORTS
(2023)
Article
Food Science & Technology
Yue Shen, Fangchao Cui, Dangfeng Wang, Tingting Li, Jianrong Li
Summary: A new QS quenching enzyme, PF-1240, was discovered in this study, which can effectively inhibit spoilage phenotype of spoilage bacteria in aquatic products and has potential applications in the preservation of aquatic products.
Article
Food Science & Technology
Wei Wei, Xinyu Hu, Sai Yang, Kaili Wang, Chunlin Zeng, Zhaozhi Hou, Henglin Cui, Shenkui Liu, Lin Zhu
Summary: This study identified the microbial groups responsible for the natural degradation of nitrite in salted radish and elucidated the associated metabolic mechanism. Halophilic archaea from pickling salt dominate the denitrification pathway, with key genera including Haloarcula, Halolamina, and Halobacterium. These archaea cope with high salt stress through a salt-in mechanism and efficiently reduce nitrite to nitrogen, providing important insights for nitrite hazard prevention in high-salinity salted vegetables.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Food Science & Technology
Guangyu Wang, Qing Li, Wenqian Tang, Fang Ma, Huhu Wang, Xinglian Xu, Weifen Qiu
Summary: The study revealed that aprD plays a crucial role in surface-associated behaviors of bacteria, impacting autoaggregation, swimming motility, and biofilm formation of a Delta aprD mutant. aprD is also involved in spoilage activities of P. fragi by influencing extracellular protease activity, total volatile basic nitrogen production, pH, and sensory traits of meat samples. These findings suggest aprD-mediated regulation profiles in P. fragi and offer new insights into chilled meat preservation.
Article
Biochemistry & Molecular Biology
Hongchang Qian, Juantao Zhang, Tianyu Cui, Lei Fan, Xudong Chen, Wenlong Liu, Weiwei Chang, Cuiwei Du, Dawei Zhang
Summary: Increasing NaCl concentration promotes corrosion of carbon steel and microbiologically influenced corrosion. However, a high concentration of chloride ions interferes with oxygen adsorption and cell attachment, leading to a reduced corrosion rate and inhibited MIC.
BIOELECTROCHEMISTRY
(2021)
Article
Immunology
Krzysztof T. Krawczyk, Camille Locht, Magdalena Kowalewicz-Kulbat
Summary: This study reveals for the first time that halophilic archaea can activate specific cells in the human immune system, leading to cytokine production and T cell activation. This finding may contribute to understanding the mechanisms by which halotherapy affects immune balance in the human body.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)