Review
Agriculture, Multidisciplinary
Jianting Ning, Mei Yang, Wanting Liu, Xue Luo, Xiqing Yue
Summary: Cow's milk is commonly used in infant formulas but can cause allergic reactions. Finding alternatives and filling the nutritional gap with human milk is crucial for infant health. Proteomic and peptidomic techniques have been used to study the differences in human, cow, and donkey milk. Donkey milk proteins are closer to human milk, have lower allergenicity, and may be developed as novel raw materials for formula milk powders.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Engineering, Chemical
Siyu Pu, Kunn Hadinoto
Summary: This study compared antisolvent and salting-out precipitations in terms of precipitation efficiency and product qualities. Antisolvent precipitation produced heavy precipitates composed of nanoparticles, while salting-out precipitation produced crystalline microparticles. At their respective optimal conditions, antisolvent precipitation had significantly higher yield than salting-out, but both precipitates exhibited comparable purity, peptide secondary structures, thermal stability, and antimicrobial activity.
Article
Chemistry, Applied
Qiang Cui, Zhiqing Zhang, Meiliang Li, Man Zhou, Xiaomeng Sun
Summary: This study analyzed the peptide profiles and potential allergenicity of milk protein concentrate hydrolysates using the peptidomics approach. The degree of hydrolysis and peptide profiles of the hydrolysates varied depending on the hydrolysis time and enzymes used. Allergic peptides were identified in the hydrolysates, but their allergenicity was significantly reduced compared to the original concentrate. This research supports the use of these hydrolysates in hypoallergenic formula foods.
Article
Chemistry, Applied
Qing Ren, Mattia Boiani, Tao He, Harry J. Wichers, Kasper A. Hettinga
Summary: This study analyzed the effects of heating on the digestion of skimmed goat milk proteins, and found that different heating temperatures affected the digestion rate, clot formation, and bioactive peptide abundance of the proteins.
Article
Chemistry, Applied
Jounghyun Um, Jean Manguy, Joao Anes, Jean-Christophe Jacquier, Daniel Hurley, Eugene T. Dillon, Kieran Wynne, Seamus Fanning, Michael O'Sullivan, Denis C. Shields
Summary: Research enriching cationic antimicrobial peptides from milk hydrolysates using pectin/alginate gels showed strong antimicrobial effects against various bacterial strains, highlighting the potential of pectin/alginate food-gels based processes as safe and cost-effective methods for separating and enriching antimicrobial peptides.
Article
Food Science & Technology
Hongji Ye, Xinyi Zhang, Yang Jiang, Min Guo, Xiaoming Liu, Jianxin Zhao, Hao Zhang, Wei Chen
Summary: This study investigated the peptidomic patterns of fermented milk by different strains of Lactobacillus delbrueckii ssp. bulgaricus and ssp. lactis before and after simulated gastrointestinal digestion. Variations in peptidomics were found among samples, with different subspecies displaying distinct peptide profiles. Digestion appeared to reduce peptidomics variations and the strain L. delbrueckii ssp. bulgaricus DQHXNS8L6 was the most efficient in generating bioactive peptides.
Article
Food Science & Technology
Shuman Gao, Yang Jiang, Xinyi Zhang, Shumao Cui, Xiaoming Liu, Jianxin Zhao, Hao Zhang, Wei Chen
Summary: This study investigated the proteolysis patterns of different Lactobacillus helveticus strains with different cell envelope proteinase (CEP) genes during milk fermentation using peptidomics. The results showed that variations in peptide patterns of casein fractions were correlated with CEP genotypes, and the bioactive peptides derived from the common hydrolysis region exhibited antihypertensive effects.
Article
Food Science & Technology
Tora Asledottir, Gerd Elisabeth Vegarud, Gianluca Picariello, Gianfranco Mamone, Tor Erling Lea, Arne Roseth, Pasquale Ferranti, Tove Gulbrandsen Devold
Summary: We investigated the peptide profiles of pea and faba bean proteins and identified over 400 unique peptides using high-resolution mass spectrometry. In silico analysis predicted that 24 peptides could potentially have bioactive properties. We also identified recognized bioactive peptides and allergens in the digests. Four selected peptides showed significant immune-modulating properties, with a specific pea peptide reducing the IL-8 response by up to 40% during IL-1 beta induction. The findings suggest the need to introduce legumes in the diet for their nutritional benefits and immune-modulatory properties, especially in Nordic countries where legumes are underutilized as a food category.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Biochemistry & Molecular Biology
Wararat Chiangjong, Jirawan Panachan, Thitinee Vanichapol, Nutkridta Pongsakul, Pongpak Pongphitcha, Teerapong Siriboonpiputtana, Tassanee Lerksuthirat, Pracha Nuntnarumit, Sarayut Supapannachart, Chantragan Srisomsap, Jisnuson Svasti, Suradej Hongeng, Somchai Chutipongtanate
Summary: HMP-S7, a novel anti-leukemic peptide with 3+ charges and an alpha helical structure, exhibits selective cytotoxic activity against leukemic cells while sparing solid malignancies and normal cells. It induces leukemic cell death by penetrating the plasma membrane to enter the cytoplasm and cause the leakage of lactate dehydrogenase. Further validation in preclinical and clinical studies is needed to confirm its anti-leukemic effects.
Article
Agriculture, Dairy & Animal Science
X. S. Liang, Y. X. Yue, Y. L. Zhao, Y. M. Guo, X. Y. Guo, B. L. Shi, S. M. Yan
Summary: This experiment aimed to study the effects of dietary concentrate-to-forage ratio on lactating donkey milk production performance, milk protein synthesis, and milk amino acid composition. The results showed that replacing low-quality roughage with alfalfa improved the protein and energy metabolism, increased milk protein synthesis efficiency, milk production efficiency, and optimized the amino acid composition of donkey milk.
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Mohan Li, Yushi Dong, Weixuan Li, Xinyu Shen, Rayhnigul Abdlla, Jiali Chen, Xueyan Cao, Xiqing Yue
Summary: This study conducted a qualitative proteomic analysis of donkey and bovine whey proteins, revealing significant differences in their composition and identifying unique whey proteins in each source. The analysis of protein functions and pathways provides a theoretical basis for the development of whey protein nutrition.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Yue Sun, Rongchun Wang, Qiming Li, Ying Ma
Summary: This study aimed to investigate the changes in protein composition and plasmin in UHT and C-UHT milk. The digestion characteristics, composition, and peptide spectrum of milk protein sterilized with the two technologies were studied using a simulated human stomach. The results showed that C-UHT milk had higher protein degradation compared to UHT milk, resulting in different derived peptides during digestion. There was no significant impact of UHT and C-UHT on the peptide spectrum of milk proteins, but C-UHT released relatively more bioactive peptides and free amino acids.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Food Science & Technology
Hao Yin, Yongli Jiang, Xuefu Zhou, Yu Zhong, Danfeng Wang, Yun Deng, Haoyan Xue
Summary: In this study, radio frequency (RF) technology was used to assist enzymatic hydrolysis, and the effects of different physical assistance methods (RF, ultrasound, and microwave) were compared. The results showed that all three methods improved hydrolysis efficiency and increased antioxidant activity of the hydrolysates. RF-assisted hydrolysis had comparable effects as microwave-assisted hydrolysis, but RF had the advantages of high penetration ability, good uniformity, and low energy consumption. Physical assistance methods were effective in improving enzymatic hydrolysis efficiency and producing novel antioxidant peptides.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Food Science & Technology
Tullia Tedeschi, Maria Aspri, Cecilia Loffi, Luca Dellafiora, Gianni Galaverna, Photis Papademas
Summary: Non-thermal processing, specifically UV-C radiation combined with turbulent flow, shows promise as a method for reducing bacterial populations in milk while preserving the bioactive properties of dairy products. In the case of freeze-dried donkey milk powder, UV-C treatment retains protein quality and bioactivities comparable to raw milk, unlike pasteurization which leads to some deterioration.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Applied
Isabela Claudia Barbosa dos Santos, Adriano Henrique do Nascimento Rangel, Claudio Vaz Di Mambro Ribeiro, Chiara Albano de Araujo Oliveira
Summary: This study evaluated the influence of lactation stage and animal age on the milk composition of grazing donkeys. The results showed that milk total solids, non-fat solids, protein, casein, and nitrogen fractions decreased as the lactation stage progressed, whereas aging was associated with increased protein and fat concentration in milk.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Biochemical Research Methods
Andrea Cerrato, Susy Piovesana, Sara Elsa Aita, Chiara Cavaliere, Simona Felletti, Aldo Lagana, Carmela Maria Montone, Celia Vargas-de-la-Cruz, Anna Laura Capriotti
Summary: This study investigated the phenolic composition of Vaccinium floribundum Kunth, a species endemic to the Andean regions. High-resolution mass spectrometry analysis revealed that Andean berries are a rich source of phenolic compounds, and the molecular transformations of these compounds during fermentation were comprehensively studied for the first time.
PHYTOCHEMICAL ANALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Andrea Cerrato, Sara Elsa Aita, Anna Laura Capriotti, Chiara Cavaliere, Angela Michela Immacolata Montone, Carmela Maria Montone, Aldo Lagana
Summary: In this study, a suspect screening approach based on high-resolution mass spectrometry was used to comprehensively characterize the short endogenous peptidome in dry-cured ham samples. A total of 236 short peptide sequences, including 173 natural short peptides and 63 sequences containing non-proteinogenic amino acids, were tentatively identified. Several modified short peptides, such as pyroglutamination and lactoyl conjugation, were also observed, suggesting reactions occurring over time.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Pilar Gonzalez-Garcia, Maria Elena Diaz-Casado, Agustin Hidalgo-Gutierrez, Laura Jimenez-Sanchez, Mohammed Bakkali, Eliana Barriocanal-Casado, Germaine Escames, Riccardo Zenezini Chiozzi, Franziska Vollmy, Esther A. Zaal, Celia R. Berkers, Albert J. R. Heck, Luis C. Lopez
Summary: The study reveals that CoQ deficiency leads to extensive changes in mitochondrial proteome and metabolism, as well as neuroinflammatory response and encephalopathic phenotype. Treatment with analogs of CoQ biosynthetic precursor partially restores CoQ metabolism and improves mitochondrial proteome and metabolism, resulting in reductions in gliosis, neuroinflammation, and spongiosis.
Article
Oncology
Damiano Caputo, Alessandro Coppola, Erica Quagliarini, Riccardo Di Santo, Anna Laura Capriotti, Roberto Cammarata, Aldo Lagana, Massimiliano Papi, Luca Digiacomo, Roberto Coppola, Daniela Pozzi, Giulio Caracciolo
Summary: In this study, a multiplexed strategy combining a nanoparticle-enabled blood test and serum levels of acute-phase proteins demonstrated the ability to distinguish pancreatic cancer patients from healthy controls. The study suggests the potential role of acute-phase proteins as biomarkers for pancreatic cancer and provides a new avenue for the development of multiplexed technologies for early cancer detection.
Article
Clinical Neurology
Francesca Magrinelli, Clarissa Rocca, Roberto Simone, Riccardo Zenezini Chiozzi, Zane Jaunmuktane, Niccolo E. Mencacci, Michele Tinazzi, Sandeep Jayawant, Andrea H. H. Nemeth, German Demidov, Henry Houlden, Kailash P. P. Bhatia
Summary: This study identified a novel exonic AluYa5 insertion causing NKX2-1-related disorder, which is challenging to detect in routine diagnostics. MEI analysis of short-read sequencing data or targeted long-read sequencing could reveal the molecular diagnosis of unresolved movement disorder cases.
MOVEMENT DISORDERS
(2023)
Article
Chemistry, Analytical
Andrea Cerrato, Chiara Cavaliere, Carmela Maria Montone, Susy Piovesana
Summary: The paper presents the preparation and characterization of a new HILIC material for the enrichment of N-linked glycopeptides. The material was prepared using specific monomer and cross-linker, and by fine-tuning the polymer properties, it displayed superior performance in terms of the number of enriched glycopeptides and selectivity. These results not only provide a new HILIC material for glycopeptide enrichment but also pave the way for the use of hydrogel materials in this field.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Miguel Ricardo Leung, Jianwei Zeng, Xiangli Wang, Marc C. Roelofs, Wei Huang, Riccardo Zenezini Chiozzi, Johannes F. Hevler, Albert J. R. Heck, Susan K. Dutcher, Alan Brown, Rui Zhang, Tzviya Zeev-Ben-Mordehai
Summary: Sperm motility is crucial for successful reproduction, but the ornamentation of axonemal microtubules and its support for motility in different fertilization environments are unclear. This study investigates native axonemal doublet microtubules (DMTs) in sea urchin and bovine sperm and identifies over 60 proteins decorating DMTs, including those linked to infertility. Through analyzing DMTs across species and cell types, the study defines core microtubule inner proteins (MIPs) and examines the evolution of the tektin bundle. Furthermore, a testis-specific serine/threonine kinase that connects DMTs to outer dense fibers in mammalian sperm is identified. The study provides structural insights into sperm evolution, motility, and dysfunction at a molecular level.
Article
Biochemical Research Methods
Andrea Cerrato, Sara Elsa Aita, Giuseppe Cannazza, Anna Laura Capriotti, Chiara Cavaliere, Cinzia Citti, Chiara Dal Bosco, Alessandra Gentili, Carmela Maria Montone, Roberta Paris, Aldo Lagana
Summary: Industrial hemp is being widely used for pharmaceutical and nutraceutical purposes, but the detailed composition of hemp remains poorly understood. In this study, the carotenoids and fat-soluble vitamins in the inflorescences of seven Italian industrial hemp varieties were analyzed. The most abundant carotenoids were found to be phytoene, phytofluene, and all-trans -a-carotene. In addition, several other carotenoids were tentatively identified using liquid chromatography coupled with photodiode-array detection.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Carmela Maria Montone, Benedetta Giannelli Moneta, Sara Elsa Aita, Anna Laura Capriotti, Andrea Cerrato, Aldo Lagan, Angela Marchetti, Susy Piovesana, Marianna Villano, Chiara Cavaliere
Summary: The presence of pharmaceuticals in the aquatic environment is mainly due to their release from wastewater treatment plants. Sulfonamides, a class of synthetic antibacterials, have often been found in surface water, contributing to antibiotic resistance. Monitoring their transformation products (TPs) is important, as they may still exert pharmaceutical activity. In this study, three commonly used sulfonamides were incubated with activated sludge, and their TPs were identified using mass spectrometry. The identified TPs were then used to analyze wastewater treatment plant samples. Acetylation was found to be the predominant transformation pathway.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Microbiology
Yvette S. Levray, Bianca Bana, Sarah J. Tarr, Emilia J. McLaughlin, Peter Rossi-Smith, Anita Waltho, Georgina H. Charlton, Riccardo Zenezini Chiozzi, Colin R. Straton, Konstantinos Thalassinos, Andrew R. Osborne
Summary: During malaria infection, the malaria parasite exports proteins into human red blood cells. Some of these proteins become embedded in membranes and are referred to as membrane proteins. The mechanism of how these membrane proteins are transported is poorly understood. This study reveals that a subset of membrane proteins enter into the lumen of the endoplasmic reticulum and may represent a unique drug target.
Article
Andrology
Min Zhang, Riccardo Zenezini Chiozzi, Elizabeth G. Bromfield, Albert J. R. Heck, J. Bernd Helms, Bart M. Gadella
Summary: This study aimed to identify the interacting partners of CRISP2. The interactions of these binding partners were investigated under different conditions. The results suggest that CRISP2 may act as a scaffold for protein complex formation and dissociation to ensure the correct positioning of proteins required for the acrosome reaction and zona pellucida penetration.
Article
Chemistry, Analytical
Andrea Cerrato, Alessandra Biancolillo, Giuseppe Cannazza, Chiara Cavaliere, Cinzia Citti, Aldo Lagana, Federico Marini, Massimo Montanari, Carmela Maria Montone, Roberta Paris, Nino Virzi, Anna Laura Capriotti
Summary: This study conducted a comprehensive analysis of industrial hemp samples from different regions in Italy and different cultivars using metabolomics. The study found that cannabinoids, flavonoids, and phenolic acids play a key role in differentiating hemp samples and determining their biological activities.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Guillermo Santos-Sanchez, Ivan Cruz-Chamorro, Carlotta Bollati, Martina Bartolomei, Justo Pedroche, Francisco Millan, Maria del Carmen Millan-Linares, Anna Laura Capriotti, Andrea Cerrato, Aldo Lagana, Anna Arnoldi, Antonio Carrillo-Vico, Carmen Lammi
Summary: This study elucidated the molecular mechanism underlying the hypocholesterolemic effects of Lupin protein hydrolysates (LPHs) in an in vivo model. The results showed that LPH treatment reduced de novo cholesterol synthesis through the regulation of HMGCoAR and LDLR protein levels. LPHs also controlled the PSCK9 signal pathway, leading to decreased cholesterol absorption. These findings highlight LPHs as a potential ingredient for developing new functional foods.
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Yinhua Pan, Quanqing Zou, Wu Yin, Zhen Huang, Yingzhu Zhao, Zongming Mo, Lihui Li, Jianrong Yang
Summary: A prognostic model of BC based on LNM-related genes was established in this study, providing guidance for prognosis evaluation and precise treatment of BC. The genes related to lymph node metastasis in BC are largely unknown and need further exploration. Searching for potential lymph node metastasis-related genes of BC will provide meaningful biomarkers for BC treatment. Our model, based on TCGA-BRCA data, effectively predicted patient outcomes and classified BC patients. The identified feature genes might have a predictive function in immunotherapy. The results of this study provide a new reference for the prognosis and treatment of BC patients with lymph node metastasis.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Qiushi Liang, Joon M. Pijnenburg, Erikjan Rijkers, Arnold G. Vulto, Ans T. van der Ploeg, Niek P. van Til, Eva C. Vlaar, Jeroen A. A. Demmers, W. W. M. Pim Pijnappel
Summary: Pompe disease is a lysosomal storage disorder characterized by glycogen accumulation in skeletal muscle, leading to profound pathology. This study showed that lentiviral gene therapy with LV-IGF2.GAAco can nearly completely correct disease-associated proteomic changes, supporting its future clinical development as a new treatment option for Pompe disease.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Chuang Liu, Wenjing Zhang, Qianli Dong, Haipeng Liu
Summary: This study fills the knowledge gap about the matrix proteins involved in the formation of mineralized exoskeletons in decapod crustaceans. By comparing with well-studied molluscan biomineralization proteins, it is proposed that decapod crustaceans evolved novel proteins while sharing some proteins with molluscs. This study provides insights into the evolution and adaptation of crustaceans and molluscs in forming their exoskeletons.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Rania D. Baka, Josipa Kules, Angelo Beletic, Vladimir Farkas, Dina Resetar Maslov, Blanka Beer Ljubic, Ivana Rubic, Vladimir Mrljak, Mark McLaughlin, David Eckersall, Zoe Polizopoulou
Summary: This study analyzed serum samples from dogs with epilepsy and identified potential markers of epilepsy, proteins involved in nerve tissue regeneration, and proteins altered by antiepileptic medication. The findings provide insight into the pathogenesis of epilepsy and the effects of medication on extracellular matrix proteins.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Viviane M. Codognoto, Fabiana F. de Souza, Thais R. Cataldi, Carlos A. Labate, Laiza S. de Camargo, Pedro H. Esteves Trindade, Roberto R. da Rosa Filho, Diego J. B. de Oliveira, Eunice Oba
Summary: This study aimed to compare urine proteomics from non- and pregnant buffaloes to identify potential biomarkers of early pregnancy. A total of 798 proteins were reported in the urine considering all groups, and the differential proteins play essential roles during pregnancy.
JOURNAL OF PROTEOMICS
(2024)