Article
Pharmacology & Pharmacy
Han Xia, Yueyang Tian, Yile Lin, Qia Huang, Yuan Xue
Summary: This study found that Insulin-like growth factor-binding protein 7 (IGFBP7) plays an important role in mediating the anabolic effects of parathyroid hormone (PTH). Intermittent PTH administration increased the expression of IGFBP7 in mesenchymal stem cells (MSCs) and pre-osteoblasts. Knockdown of IGFBP7 interrupted the anabolic effects of PTH, while supplementation with IGFBP7 protein enhanced the bone-forming efficacy of PTH. In a mouse model of fracture, administration of IGFBP7 along with PTH accelerated bone healing.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Liang Ma, Zeyue Gao, Jiegen Wu, Bijunyao Zhong, Yuchen Xie, Wen Huang, Yihan Lin
Summary: The coactivator p300 can dynamically coordinate its functions by forming clusters with transcription factors, enabling spatially defined activation of its catalytic activity. This co-condensation mechanism modulates the transcriptional initiation rate and burst duration of target genes, contributing to nonlinear gene regulation. The results suggest a mechanism where TF and p300 co-condensation contributes to transcriptional bursting regulation and cooperative gene control.
Review
Oncology
Swarnalatha Manickavinayaham, Briana K. Dennehey, David G. Johnson
Summary: RB and E2F proteins collaborate to regulate the cell cycle in eukaryotes and also have important functions in DNA repair. In some organisms, other proteins have taken over the cell cycle regulation function of RB and E2F. Apart from cell cycle regulation, E2F and RB homologs in various organisms also possess non-canonical functions.
Article
Biology
Deng Tan, Rui Chen, Yuejian Mo, Shu Gu, Jiao Ma, Wei Xu, Xibin Lu, Huiyu He, Fan Jiang, Weimin Fan, Yili Wang, Xi Chen, Wei Huang
Summary: Noise in gene expression is critical for cellular processes, and this study explored the mechanisms behind large fluctuations induced by single transcriptional activators. The findings suggest that an interplay between transcriptional activators and co-activators/histone acetyltransferases contributes to heterogeneity in gene expression. Modulation of transcriptional activator binding can reduce noise, potentially by altering epigenetic states.
Article
Biochemistry & Molecular Biology
R. Hari Krishnan, Lakshana Sadu, R. L. Akshaya, K. Gomathi, I. Saranya, Das Udipt Ranjan, Sneha Satishkumar, N. Selvamurugan
Summary: Parathyroid hormone (PTH) regulates bone remodeling genes by enhancing Runx2 activity in osteoblasts. This study showed that PTH can regulate Runx2 activity through non-coding RNAs (ncRNAs)-mediated p300 expression. Potential regulation of p300 by miR-130b-5p and circ_CUX1 was identified. The circ_CUX1/miR-130b-5p/p300 axis may provide new insights for diagnostic and therapeutic approaches in bone-related diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Multidisciplinary Sciences
Jin Wu, Yingying Han, Hao Xu, Hongyang Sun, Rui Wang, Haigang Ren, Guanghui Wang
Summary: This study investigates the role of chaperone-mediated autophagy (CMA) in neuroinflammation and its impact on microglia activation and neuronal damage. The researchers found that CMA activation can inhibit microglial activation and inflammation through the p300-associated NF-kappa B signaling pathway. Additionally, they demonstrated that CMA dysfunction worsens microglial activation and inflammation. These findings provide insight into the mechanistic link between CMA and neuroinflammation.
Article
Biochemistry & Molecular Biology
Zehua Zou, Ruixuan Liu, Yiwen Wang, Yufei Xing, Zuowei Shi, Kaifu Wang, Daming Dong
Summary: IL1RN is constantly expressed in MC3T3-E1 and C3H10T1/2 cells, with its overexpression enhancing osteogenic effects. It interacts with integrin beta 3 to activate beta-catenin signaling, ultimately regulating osteoblast differentiation. This study suggests IL1RN as a potential therapeutic target for osteoporosis treatment.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2021)
Article
Endocrinology & Metabolism
Linlin Zhang, Kecheng Zhu, Jingzun Xu, Xiaojing Chen, Chunxiang Sheng, Deng Zhang, Yuying Yang, Lihao Sun, Hongyan Zhao, Xiao Wang, Bei Tao, Libin Zhou, Jianmin Liu
Summary: CBP and p300, as important histone acetyltransferases, have been found to affect bone density and strength. The study further demonstrates that CBP/p300 HAT regulates osteogenic gene expression by modulating transcription of beta-catenin and Stat1. These findings reveal an epigenetic mechanism responsible for maintaining bone homeostasis.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Developmental Biology
Susanna Fraguas, Sheila Carcel, Coral Vivancos, Ma Dolores Molina, Jordi Gines, Judith Mazariegos, Thileepan Sekaran, Kerstin Bartscherer, Rafael Romero, Francesc Cebria
Summary: CBP/p300 play important roles in stem cell proliferation and differentiation. The study in planarian Schmidtea mediterranea identified 5 distinct Smed-cbp genes with different expression patterns. Functional analysis showed the essential role of Smed-cbp-2 in stem cell maintenance and the impact of silencing Smed-cbp-3 on the differentiation of various cell lineages. Furthermore, the predicted interactomes of CBP-2 and CBP-3 were analyzed to better understand their functions in planarian stem cell biology.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Alexandra-Gela Lazar, Mihaela-Loredana Vlad, Adrian Manea, Maya Simionescu, Simona-Adriana Manea
Summary: Accumulating evidence supports the involvement of histone acetylation-based epigenetic mechanisms in the pathoetiology of diabetes-associated micro-/macrovascular complications, with a focus on diabetic kidney disease. Pharmacological inhibition of p300/CBP shows promise in down-regulating the expression of Nox subtypes, inflammation, and fibrosis in the diabetic kidney, potentially reducing the burden of DKD.
Article
Medicine, Research & Experimental
N. Akshaya, N. Srinaath, M. Rohini, R. Ilangovan, N. Selvamurugan
Summary: In this study, the authors investigated the role of PTH-responsive miR-290 in the regulation of Runx2-mediated MMP-13 expression in rat osteoblastic cells. The results suggested that miR-290 may serve as a molecular marker or target for bone and bone-related diseases.
CURRENT MOLECULAR MEDICINE
(2022)
Article
Oncology
Yongguang Zhang, Danfeng Lin, Yongwei Zheng, Yuhong Chen, Mei Yu, Dongya Cui, Miaohui Huang, Xinlin Su, Yong Sun, Yabing Chen, Zhijian Qian, Karen-Sue Carlson, Renren Wen, Demin Wang
Summary: Deletion of miR-9-1 results in smaller size and postweaning lethality in mice, as well as lymphopenia and impaired osteoblastic bone formation.
Review
Nutrition & Dietetics
James C. Fleet
Summary: Vitamin D plays a critical role in regulating calcium and bone homeostasis, especially in the absorption of calcium in the intestines. The intestinal absorption of calcium is regulated by the hormone 1,25 dihydroxyvitamin D, which activates gene transcription by binding to the intestinal vitamin D receptor. Various physiological and disease states can affect the regulation of intestinal calcium absorption by vitamin D.
Article
Biochemistry & Molecular Biology
Meghan E. Breen, Stephen T. Joy, Omari J. Baruti, Matthew S. Beyersdorf, Madeleine J. Henley, Samantha N. De Salle, Peter D. Ycas, Ayza Croskey, Tomasz Cierpicki, William C. K. Pomerantz, Anna K. Mapp
Summary: Natural product garcinolic acid blocks CBP/p300 KIX PPI network by strong interaction with a non-canonical binding site, inhibiting KIX-dependent transcription in leukemia. It selectively engages CBP/p300 KIX domain, disrupting its protein-protein interactions and downregulating essential transcriptional circuits for cMyb-dependent leukemias.
Review
Biochemistry & Molecular Biology
Hadla Hariri, Rene St-Arnaud
Summary: This review highlights recent research on the regulatory functions of ubiquitin-specific peptidases (USPs) in osteoblasts and provides insight into the molecular mechanisms governing their actions. It also discusses the role of USP53 in osteoblasts and the complex layers of regulation exerted by USPs on osteoblast signaling. Further studies using knockout mouse models are necessary for a full understanding of the mechanisms underpinning USPs actions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yiwen Qin, Yuanzhen Peng, Wei Zhao, Jianping Pan, Hanna Ksiezak-Reding, Christopher Cardozo, Yingjie Wu, Paola Divieti Pajevic, Lynda F. Bonewald, William A. Bauman, Weiping Qin
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Endocrinology & Metabolism
Niloufar Ansari, Patricia W. M. Ho, Blessing Crimeen-Irwin, Ingrid J. Poulton, Athena R. Brunt, Mark R. Forwood, Paola Divieti Pajevic, Jonathan H. Gooi, T. John Martin, Natalie A. Sims
JOURNAL OF BONE AND MINERAL RESEARCH
(2018)
Article
Medicine, Research & Experimental
Guojun Tong, Yue Meng, Song Hao, Shaoyu Hu, Youhua He, Wenjuan Yan, Dehong Yang
MEDICAL SCIENCE MONITOR
(2017)
Review
Endocrinology & Metabolism
Paola Divieti Pajevic, Daniela S. Krause
Article
Orthopedics
Wei Li, Liang Yuan, Guojun Tong, Youhua He, Yue Meng, Song Hao, Jianting Chen, Jun Guo, Richard Bringhurst, Dehong Yang
BMC MUSCULOSKELETAL DISORDERS
(2018)
Article
Endocrinology & Metabolism
Christopher Dedic, Tin Shing Hung, Alan M. Shipley, Akira Maeda, Thomas Gardella, Andrew L. Miller, Paola Divieti Pajevic, Joseph G. Kunkel, Alessandro Rubinacci
Article
Endocrinology & Metabolism
Keertik Fulzele, Christopher Dedic, Forest Lai, Mary Bouxsein, Sutada Lotinun, Roland Baron, Paola Divieti Pajevic
Article
Clinical Neurology
Dehong Yang, Xiaoliang Wu, Minghui Zheng, Jixing Wang
WORLD NEUROSURGERY
(2018)
Article
Biochemistry & Molecular Biology
Ningyuan Sun, Yuhei Uda, Ehab Azab, Alejandro Kochen, Roberto Nunes Campos E. Santos, Chao Shi, Tokio Kobayashi, Marc N. Wein, Paola Divieti Pajevic
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Endocrinology & Metabolism
Yangyang Ma, Xiaoliang Wu, Xiao Xiao, Yao Ma, Lan Feng, Wenjuan Yan, Jianting Chen, Dehong Yang
Article
Endocrinology & Metabolism
Youhua He, Minghan Li, Guojun Tong, Yue Meng, Song Hao, Shaoyu Hu, Wenjuan Yan, Dehong Yang
Article
Clinical Neurology
Xiaoliang Wu, Yangyang Ma, Ruoting Ding, Xiao Xiao, Dehong Yang
Summary: The study found that the reoperation rate of untreated asymptomatic lumbar disc herniation (asLDH) after open fusion or percutaneous endoscopic lumbar discectomy (PELD) is closely related to the severity of herniation. A high possibility of reoperation should be clearly discussed with the patient when facing an asLDH graded as G2, regardless of open fusion or PELD techniques.
Article
Orthopedics
Shangbo Niu, Dehong Yang, Yangyang Ma, Shengliang Lin, Xuhao Xu
Summary: This study aimed to investigate the impact of internal fixation removal on the quality of life in patients who underwent successful lumbar intervertebral fusion. The results showed that internal fixation removal could improve the quality of life in patients experiencing discomfort. However, in patients without pre-surgery discomfort, the removal of internal fixation had limited or even negative effects, and should not be performed routinely.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Engineering, Biomedical
Zhang Yanli, Mo Jiayao, Zheng Chunqing, Zeng Yuting, Zhou Zhiyan, Zhang Yulin, Li Minghan, Shao Longquan, Yang Dehong, Yan Wenjuan
Summary: The dynamic changing of extracellular matrix (ECM) can activate the driving force of osteoblast cells and accelerate bone regeneration. In this study, a novel peptide MY-1 is designed and synthesized, and nano-hydroxyapatite (nHA) is used as the carrier for sustained release of MY-1. The results show that sustained release of MY-1 regulates the synthesis and secretion of ECM, promoting cell migration and osteogenic differentiation, which benefits bone regeneration.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Cell Biology
Chunhao Zhou, Donghua Guan, Jialiang Guo, Shangbo Niu, Zhihai Cai, Chengfu Li, Chenghe Qin, Wenjuan Yan, Dehong Yang
Summary: This study demonstrated that MY-1 accelerates skin wound re-epithelialization by inducing keratinocyte migration and EMT via PTHR1-PI3K/AKT axis activation.
CELL COMMUNICATION AND SIGNALING
(2023)