Review
Plant Sciences
Shumei Fang, Xue Hou, Xilong Liang
Summary: SUMOylation is a crucial posttranslational modification in eukaryotes, involved in metabolic regulation under various stresses. SIZ1, a SUMO E3 ligase, plays a vital role in substrate-specific recognition and facilitates the binding of SUMO proteins to target proteins. This review provides insights into the characteristics of SUMOylation and the role of SIZ1 in plant adaptation to abiotic stresses, offering valuable references for crop improvement and future research on plant responses to environmental stress.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Review
Biochemistry & Molecular Biology
Nieves Lara-Urena, Vahid Jafari, Mario Garcia-Dominguez
Summary: SUMOylation is an important post-translational modification that regulates diverse physiological processes. Dysregulation of the SUMO pathway, involving proteases and ligases, is associated with cancer initiation and progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Jie Li, Gregory J. Krause, Qi Gui, Susmita Kaushik, Gergely Rona, Qingyue Zhang, Feng-Xia Liang, Avantika Dhabaria, Carlos Anerillas, Jennifer L. Martindale, Nikita Vasilyev, Manor Askenazi, Beatrix Ueberheide, Evgeny Nudler, Myriam Gorospe, Ana Maria Cuervo, Michele Pagano
Summary: This study identifies a new function for mammalian SKP1 in the endolysosomes, which is independent of its established role as a component of ubiquitin ligases. SKP1 is found to be phosphorylated under nutrient-poor conditions, promoting V-ATPase assembly and acidification of late endosomes for degradation. Under nutrient-rich conditions, phosphorylated SKP1 is SUMOylated and allows unconventional secretion through interaction with SEC22B.
Review
Cell Biology
Jara Lascorz, Joan Codina-Fabra, David Reverter, Jordi Torres-Rosell
Summary: Post-translational modification by SUMO proteins involves non-covalent interactions with other proteins, which play crucial roles in regulating protein activity, localization, and stability.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Plant Sciences
Ruiqiang Lai, Jieming Jiang, Jun Wang, Jinju Du, Jianbin Lai, Chengwei Yang
Summary: SUMOylation is a crucial post-translational modification that regulates protein substrates. The maize SIZ1 homologs ZmSIZ1a, ZmSIZ1b, and ZmSIZ1c were functionally characterized in this study, showing potential roles in development regulation and stress responses. Overexpression of ZmSIZ1c in Arabidopsis resulted in early flowering and improved salt tolerance, suggesting distinct functions among the ZmSIZ1 members.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Samuel Smalley, Hanjo Hellmann
Summary: This review provides an overview of how ubiquitin and similar proteins modify target proteins in plants to rapidly change their stability and activity, with a focus on their contribution to stress tolerance. The study also explores the potential use of these pathways for generating more robust crop plants to cope with stress situations.
Review
Biochemistry & Molecular Biology
Nicolette S. Jansen, Alfred C. O. Vertegaal
Summary: SUMO polymers regulate various nuclear processes through dynamic signal transduction, including protein degradation. The fate of target proteins is influenced by the structure of SUMO polymers. Recent studies have demonstrated the diversity of SUMO polymer signal transduction for proteolytic and nonproteolytic purposes.
TRENDS IN BIOCHEMICAL SCIENCES
(2021)
Article
Plant Sciences
Jieming Jiang, Yun Xie, Jinju Du, Chengwei Yang, Jianbin Lai
Summary: SUMOylation plays a crucial role in regulating diverse cellular processes in eukaryotes. OsMMS21, a SUMO E3 ligase in rice, is involved in developmental processes and auxin signaling transduction. The OsMMS21 mutant displays insensitivity to auxin, resulting in a short-root and dwarfism phenotype.
JOURNAL OF PLANT PHYSIOLOGY
(2021)
Article
Cell Biology
Vasco C. Silveirinha, Hong Lin, Shota Tanifuji, Sumiko Mochida, Graeme S. Cottrell, Helena Cimarosti, Gary J. Stephens
Summary: SUMOylation is an important post-translational modification process that involves the covalent attachment of SUMO protein to target proteins. The study found that SUMO-1 protein can modulate the function of the Cav2.2 voltage-gated calcium channel, affecting neurotransmitter release and providing novel targets for SUMOylation pathways.
Article
Biochemistry & Molecular Biology
Jiawei Xiong, Fabin Yang, Fan Wei, Feng Yang, Honghui Lin, Dawei Zhang
Summary: HLS1 plays a key role in the development of the apical hook in plants, and SIZ1 regulates the rapid opening of the apical hook by mediating HLS1 SUMOylation. The opening of the apical hook is associated with a decrease in SIZ1 transcript levels, leading to reduced HLS1 SUMOylation. In addition, HY5 promotes the rapid opening of the apical hook by inhibiting SIZ1 transcription.
Article
Cell Biology
Sujuan Gao, Xueqin Zeng, Jianhao Wang, Yingchao Xu, Chunwei Yu, Yishui Huang, Feng Wang, Keqiang Wu, Songguang Yang
Summary: The study reveals that the Arabidopsis SUMO E3 ligase SIZ1 interacts with HDA6 and negatively regulates the function of HDA6 by affecting the acetylation level of histone H3 and the binding of HDA6 to its target genes.
Article
Biochemistry & Molecular Biology
Luyao Wei, Wantao Wang, Junxia Yao, Zhengyu Cui, Zihang Xu, Hanqing Ding, Xiaojun Wu, Deheng Wang, Jia Luo, Zun-Ji Ke
Summary: This study identifies protein activator of the interferon-induced protein kinase (PACT) as a key player in basal-like breast cancer (BLBC) metastasis. High expression of PACT is associated with poor prognosis in BLBC patients. It is found that PACT regulates BLBC metastasis by interacting with SUMO-conjugating enzyme Ubc9.
Article
Multidisciplinary Sciences
Terry R. Suk, Marilyn T. Tirard, Zoe A. Fisk, Miso Mitkovski, Haley M. Geertsma, Jean-Louis A. Parmasad, Meghan M. Heer, Steve M. Callaghan, Fritz Benseler, Nils Brose, Marilyn Tirard, Maxime W. C. Rousseaux
Summary: SUMOylation is a protein modification widely present in evolutionarily conserved eukaryotes and has broad biological significance. To differentiate between SUMO paralogs and uncover their specific functions in vivo, His6-HA-Sumo2 and HA-Sumo2 knockin mouse lines were generated, expanding upon the existing His6-HA-Sumo1 mouse line. By utilizing the specificity of the HA epitope, regional differences in Sumo1 and Sumo2 expression were observed through whole-brain imaging. Immunoprecipitation coupled with mass spectrometry identified shared and specific neuronal targets of Sumo1 and Sumo2, and proximity ligation assays provided insight into the subcellular distribution of neuronal Sumo2-conjugates. These mouse models and associated datasets provide a powerful framework to determine the native SUMO code in cells of the central nervous system.
Article
Biochemistry & Molecular Biology
Ayan Chanda, Yoshiho Ikeuchi, Kunal Karve, Anusi Sarkar, Amrita Singh Chandhoke, Lili Deng, Azad Bonni, Shirin Bonni
Summary: Studies show that SUMO E3 ligases PIAS1 and TIF1γ collaborate to promote the SUMOylation of SnoN, suppressing EMT in breast cell-derived tissue organoids. These E3 ligases act in an interdependent manner in the regulation process.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Biochemistry & Molecular Biology
Maria Elisa Sabatini, Micaela Compagnoni, Fausto Maffini, Claudia Miccolo, Fabio Pagni, Mariano Lombardi, Virginia Brambilla, Daniela Lepanto, Marta Tagliabue, Mohssen Ansarin, Simona Citro, Susanna Chiocca
Summary: Functional loss of E-cadherin, often occurring through proteolytic cleavage, is common during tumor progression. This downregulation leads to a more malignant phenotype by promoting Epithelial to Mesenchymal Transition (EMT). The UBC9/SUMO pathway has been shown to be involved in regulating EMT in different cancers. In this study, increased expression of UBC9 was found in the progression of Head and Neck Cancer (HNC), and a role for UBC9/SUMO in inhibiting HPV-mediated E-cadherin cleavage in HNC was uncovered.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
S. Stern, R. Santos, M. C. Marchetto, A. P. D. Mendes, G. A. Rouleau, S. Biesmans, Q-W Wang, J. Yao, P. Charnay, A. G. Bang, M. Alda, F. H. Gage
MOLECULAR PSYCHIATRY
(2018)
Article
Genetics & Heredity
Patrick Torbey, Elodie Thierion, Samuel Collombet, Anne de Cian, Carole Desmarquet-Trin-Dinh, Mathilde Dura, Jean-Paul Concordet, Patrick Charnay, Pascale Gilardi-Hebenstreit
Article
Oncology
Katarzyna J. Radomska, Fanny Coulpier, Aurelie Gresset, Alain Schmitt, Amal Debbiche, Sophie Lemoine, Pierre Wolkenstein, Jean-Michel Vallat, Patrick Charnay, Piotr Topilko
Article
Cell Biology
Noelia Luna-Pelaez, Rosana March-Diaz, Maria Ceballos-Chavez, Jose A. Guerrero-Martinez, Paolo Grazioli, Pablo Garcia-Gutierrez, Thomas Vaccari, Valentina Massa, Jose C. Reyes, Mario Garcia-Dominguez
CELL DEATH & DISEASE
(2019)
Article
Biochemistry & Molecular Biology
M. Moreno-Onate, A. M. Herrero-Ruiz, M. Garcia-Dominguez, F. Cortes-Ledesma, J. F. Ruiz
JOURNAL OF MOLECULAR BIOLOGY
(2020)
Article
Cell Biology
Juan F. Correa-Vazquez, Francisco Juarez-Vicente, Pablo Garcia-Gutierrez, Sina Barysch, Frauke Melchior, Mario Garcia-Dominguez
Summary: Post-translational modification through Sumo plays a crucial role in regulating neurogenesis. The sumoylation of key proteins can either positively or negatively affect the process of neurogenesis, highlighting the importance of balanced sumoylation for proper development.
CELL DEATH & DISEASE
(2021)
Article
Chemistry, Multidisciplinary
Maria Jose Garcia-Jimenez, Sergio Gil-Caballero, Susana Maza, Francisco Corzana, Francisco Juarez-Vicente, Jonathan R. Miles, Kazuma Sakamoto, Kenji Kadomatsu, Mario Garcia-Dominguez, Jose L. de Paz, Pedro M. Nieto
Summary: This study illustrates the interactions between Midkine and chondroitin sulfate, demonstrating that their basic shape is controlled by the geometry of the glycosidic linkages. The ligand orientation within the complex is shown to be highly variable, with Docking analysis confirming the participation of aromatic residues from Midkine. Additionally, the study tested the biological activity of increasing MK expression using CS tetrasaccharides, enhancing the growth stimulation effect of MK in NIH3T3 cells.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Editorial Material
Cell Biology
Cristina Gervasini, Mario Garcia-Dominguez, Valentina Massa
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Nieves Lara-Urena, Mario Garcia-Dominguez
Summary: The recent COVID-19 pandemic has caused a significant global impact, with research showing an interaction between the virus' E protein and BET proteins, providing new insights into viral infection studies.
Review
Biochemistry & Molecular Biology
Pablo Garcia-Gutierrez, Mario Garcia-Dominguez
Summary: CdLS is a human developmental syndrome that may be a transcriptomopathy related to transcriptional regulation, altered cohesin complex function, and mutations in NIPBL. The pathogenesis of CdLS is likely primarily based on changes in gene expression programs.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Nieves Lara-Urena, Vahid Jafari, Mario Garcia-Dominguez
Summary: SUMOylation is an important post-translational modification that regulates diverse physiological processes. Dysregulation of the SUMO pathway, involving proteases and ligases, is associated with cancer initiation and progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Biochemistry & Molecular Biology
Francisco Gallardo-Chamizo, Nieves Lara-Urena, Juan F. Correa-Vazquez, Jose C. Reyes, Benoit R. Gauthier, Mario Garcia-Dominguez
Article
Developmental Biology
Gaelle Odelin, Emilie Faure, Fanny Coulpier, Maria Di Bonito, Fanny Bajolle, Michele Studer, Jean-Francois Avierinos, Patrick Charnay, Piotr Topilko, Stephane Zaffran