Review
Biochemistry & Molecular Biology
Raymond B. Yu, Joselito P. Quirino
Summary: This review examines the use of bile salts as chiral selectors in chiral micellar electrokinetic chromatography (MEKC) over the past 20 years, discussing their aggregation in chiral recognition and separation, as well as their use in combination with other chiral selectors. Advanced techniques like partial-filling MEKC, stacking, and single-drop microextraction are considered, along with potential applications to real samples such as enantiomeric impurity analysis.
Article
Biochemistry & Molecular Biology
Shaoqiang Hu, Tao Sun, Rui Li, Dongdong Zhang, Yonghua Zhang, Zhuo Yang, Ge Feng, Xuming Guo
Summary: This study measured and compared the parameters and results of different bile salts in the separation of PALO stereoisomers by MEKC. The structure has a significant impact on the separation, with the steroidal ring of bile salts having a greater influence than the side chain structure. A model was introduced to explain the differences in separation results caused by different bile salts.
Article
Chemistry, Analytical
Raymond B. Yu, Joselito P. Quirino
Summary: Pseudophase microextraction (PPME) is presented as a simple in-line sample concentration technique in micellar electrokinetic chromatography (MEKC). Compared to popular electric-field driven stacking techniques in MEKC, such as sweeping, PPME is pressure-driven and can greatly improve peak heights for target analytes. The entire capillary is loaded with the sample and undergoes pressure-driven concentration, leading to effective detection of low levels of analytes in drinking water and natural water samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Yikun Liu, Wei Zhou, Wenqi Sun, Zilin Chen
Summary: Micellar electrokinetic chromatography (MEKC) is an efficient and selective separation technique with simple preparation process. By hyphenating MEKC with mass spectrometry (MS) using volatile ammonium perfluorooctanoate as a surfactant, this study successfully analyzed two herbicides in lake water samples.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Food Science & Technology
Feiya Sheng, Songsong Wang, Xiao Luo, Jianbo Xiao, Linfeng Hu, Peng Li
Summary: This study establishes a micellar electrokinetic chromatography (MEKC) method for the analysis of nucleosides and bases in Ganoderma, and successfully applies it in real sample analysis. The results indicate significant variation in the contents of nucleosides and bases in different Ganoderma samples, with geographical origin being a key factor affecting their accumulation.
FOOD SCIENCE AND HUMAN WELLNESS
(2022)
Article
Biochemical Research Methods
Muhandiramge Ranasinghe, Hiranya Karunaratne, Joselito P. Quirino
Summary: Analytical performance of RP-LC and Sweeping-MEKC methods were compared for the determination of coumarin in complex curry samples. RP-LC showed better linear range and limit of quantitation, while Sweeping-MEKC demonstrated higher limit of detection.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Samuel Bernardo-Bermejo, Sandra Adamez-Rodriguez, Elena Sanchez-Lopez, Maria Castro-Puyana, Maria Luisa Marina
Summary: This study developed a chiral methodology for the separation of the four stereo-isomers of 4-hydroxyproline using capillary electrophoresis. Methyl-gamma-CD was chosen as the chiral selector to separate the 4-hydroxyproline stereoisomers in a phosphate buffer. The developed method showed potential for quality control of nutricosmetic supplements, with the ability to detect enantiomeric impurities and accurately determine the content of trans-4-L-Hyp.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemical Research Methods
Raymond B. Yu, Joselito P. Quirino
Summary: The application of ionic liquids as additives in EKC plays an important role in chiral separations, as evidenced by numerous research articles. Studies show that the use of ionic liquids can improve separation efficiency, with further research needed to validate their mechanisms of action.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Krzesimir Ciura, Monika Pastewska, Szymon Ulenberg, Hanna Kapica, Piotr Kawczak, Tomasz Baczek
Summary: The study focuses on developing new micellar electro-kinetic chromatographic methods for rapid assessment of complex biological properties, aiming to characterize retention mechanisms and evaluate predictive capabilities for intestinal absorption. Using a set of 45 compounds and quantitative structure-retention relationship (QSRR) approach, the support vector machine (SVM) model was found to have better predictive abilities.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Huynh N. P. Dang, Joselito P. Quirino
Summary: In this study, modern reversed-phase high performance liquid chromatography (HPLC) and stacking-micellar electrokinetic chromatography (MEKC) methods were developed and compared for the determination of alkenylbenzenes in food flavouring ingredients. The HPLC method showed better analytical performance and faster total run time compared to the stacking-MEKC method. The developed analytical methodology was successfully applied to actual food samples, which is crucial for future studies on determining alkenylbenzenes in food.
Article
Chemistry, Analytical
Krzesimir Ciura, Izabela Fryca, Maciej Gromelski
Summary: Capillary electrophoresis (CE) is widely used in various laboratories. This study aims to fill the gap in understanding the quantitative structure retention relationship (QSRR) in the cetrimonium bromide (CTAB) micellar electrokinetic chromatography (MEKC) system. The results show that machine learning models, specifically GA-PLS, can be used to predict retention in the CTAB-MEKC system.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Hanan Alatawi, Anna Hogan, Ibtihaj Albalawi, Samia Alsefri, Eric Moore
Summary: Recently, the increased use of non-steroidal anti-inflammatory drugs (NSAIDs) in humans and animals has raised concerns about their impact on aquatic environments. In this study, a highly efficient and sensitive micellar electrokinetic chromatography (MEKC) method was developed to detect five NSAIDs in environmental water samples. The optimized method showed excellent separation using a borate buffer, SDS, and methanol, and achieved high theoretical plates and great sensitivity for NSAIDs. Furthermore, the solid phase extraction method was successfully validated for wastewater samples with excellent recovery rates.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Laura Garcia-Cansino, Karina Boltes, Maria Luisa Marina, Maria Angeles Garcia
Summary: Two chiral methods for the separation of ibrutinib enantiomers were developed using Electro-kinetic Chromatography. The use of a single cyclodextrin system achieved a separation time of 4.2 min with an enantioresolution value of 1.5. The addition of a chiral ionic liquid to the cyclodextrin system improved the separation, resulting in a separation time of 8.1 min and an enantioresolution value of 3.3.
Article
Biochemical Research Methods
Maria Angeles Garcia, Sara Jimenez-Jimenez, Maria Luisa Marina
Summary: This work developed an Electrokinetic Chromatography (EKC) method that successfully separated the four stereoisomers of the acetamide herbicide dimethenamid. Different anionic cyclodextrins (CDs) were screened, and the use of a dual CD system improved the chiral separation. The addition of deep eutectic solvents showed beneficial effects on the separation in terms of resolution.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Hai Yen Ta, Lucie Perquis, Stephane Balayssac, Christophe Dejugnat, Alexandre Wodrinski, Fabrice Collin, Veronique Gilard, Francois Couderc
Summary: Ammonium perfluorooctanoate (APFOA) was used as a surfactant to separate free unsaturated C18 fatty acids by micellar electrokinetic chromatography. The separation conditions were optimized and the process of separation was described.
Article
Engineering, Environmental
Yuhui Liu, Yaning Lu, Shuang Zhang, Xiaoyan Li, Zhibin Zhang, Liya Ge, MengYu Chang, Yunhai Liu, Grzegorz Lisak, Sheng Deng
Summary: In this study, an amphiphilic ligand NND was successfully synthesized and used to enhance the adsorption capacity of MoS2 particles for UO22+ by replacing H2O through hydrogen bonding. The NND-modified MoS2 showed higher adsorption capacity and stronger stability under extreme conditions.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Medicine, Research & Experimental
Chen Huei Leo, Jamie Li Min Ou, Eng Shi Ong, Cheng Xue Qin, Rebecca H. Ritchie, Laura J. Parry, Hooi Hooi Ng
Summary: This study evaluates the protective effects of relaxin on diabetic kidney complications. The results show that relaxin treatment can reduce key markers of kidney fibrosis, oxidative stress, and inflammation in a diabetic mouse model, and increase the levels of bile acid metabolites. Therefore, relaxin may serve as an adjunctive treatment for diabetic kidney complications.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Editorial Material
Biochemistry & Molecular Biology
Eng Shi Ong
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Analytical
Eng Shi Ong
Summary: Bioactive compounds in functional foods, medicinal plants and others are considered valuable for their wide range of bioactivity. Urine is an effective biofluid for evaluating and monitoring changes in homeostasis and metabolism. The review discusses the formation of urine, sampling and analysis of urinary metabolites, and recent developments in metabolite profiling. Advances in LC/MS/MS, chemical fragmentation libraries, computational resources, and data analysis approaches have made urine profiling an attractive option. Profiling urinary metabolites provides insights into diet-related biomarkers and their biological effects.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2023)
Review
Food Science & Technology
Chen Huei Leo, Eng Shi Ong
Summary: Functional foods and their by-products have various bioactive components that offer health benefits and can enhance public health and well-being. Environmentally friendly, sustainable, and cost-effective methods are needed to extract flavonoids, phenolic compounds, and other substances from functional food and agri-food wastes to achieve a circular economy. This research covers extraction methods that eliminate the use of organic solvents and are suitable for laboratory and commercial production. Analytical methods are used to determine marker or bioactive compounds and chemical fingerprints to control the composition variation of functional food products. Combining chemical standardization with antioxidant assays provides information on extract quality. Cellular models and other experimental endpoints, such as metabolomics, are used to validate the antioxidant properties of the extracts.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Review
Environmental Sciences
Maria Hasnain, Rida Zainab, Faraz Ali, Zainul Abideen, Jean Wan Hong Yong, Ali El-Keblawy, Saud Hashmi, Emanuele Radicetti
Summary: CO2 emissions from fossil fuels and coal combustion contribute to global climate change and warming, while heavy metals and metalloids pose a threat to ecological functionality and human life. Microalgae thrive in harsh environments and offer environmentally friendly and cost-effective CO2 remediation solutions. The interaction between microalgae and bacteria can enhance their performance in wastewater treatment and algal production systems.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Biochemistry & Molecular Biology
Okanlawon L. Jolayemi, Ali H. Malik, Ramesh R. Vetukuri, Ganapathi V. Saripella, Pruthvi B. Kalyandurg, Tobias Ekblad, Jean W. H. Yong, Marie E. Olsson, Eva Johansson
Summary: Protein-based biostimulants (PBBs) enhance plant development by upregulating genes related to protein and photosynthesis. Hydrolyzed wheat gluten (HWG) and potato protein film (PF) were effective PBBs for promoting sugar beet growth and improving protein-related traits. Sucrose accumulation in the roots was mainly influenced by the availability of nitrogen.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Sakeena Si Yu Tan, Meyammai Shanmugham, Yu Ling Chin, Jia An, Chee Kai Chua, Eng Shi Ong, Chen Huei Leo
Summary: This study extracts phytochemical compounds from mangosteen pericarp using pressurized hot water extraction (PHWE) and investigates its biological effects on endothelial cells using RNA sequencing. Results show that extraction at a temperature of 120 degrees Celsius yields the highest concentrations of key phytochemical compounds and improves antioxidant capacity. Treatment of endothelial cells with the extract activates the AMPK signaling pathway and affects biological processes related to steroid biosynthesis and metabolism. Overall, this study provides important insights into the potential beneficial effects of phytochemical compounds from mangosteen pericarp on cardiometabolic health.
Article
Engineering, Biomedical
Jian Da Tan, Cheng Pau Lee, Su Yi Foo, Joseph Choon Wee Tan, Sakeena Si Yu Tan, Eng Shi Ong, Chen Huei Leo, Michinao Hashimoto
Summary: This study explores the use of 3D printing technology to transform orange peel waste into edible, healthy snacks, aiming to address the detrimental environmental and economic impacts of discarding such waste. Through direct ink writing, edible constructs containing orange peel waste were successfully created. Biochemical analysis and cell viability tests revealed that the nutritional and antioxidant content of the orange peel waste was not affected during the manufacturing process. This study highlights the potential of revalorizing food waste and achieving more sustainable and circular food manufacturing and consumption through 3D printing.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Forestry
Aobing He, Zhibo Ma, Yunfei Li, Chen Huang, Jean Wan Hong Yong, Jian Huang
Summary: Soil salinity is a major constraint for jujube production in China. Wild jujube is used as the rootstock to overcome saline conditions. This study investigated the adaptive mechanism of wild jujube under salt stress through spatiotemporal, physiological, and transcriptomic analyses. The results showed that salt treatments had dose and duration effects on biomass, photosynthesis, and ion accumulation. Differential expression genes were identified and functional enrichment analysis revealed altered physiological activities. Certain genes, including nitrogen and potassium transporters and WRKY/MYB/bHLH transcription factors, were identified as hub genes responding to salt stress.
Article
Biochemistry & Molecular Biology
Bushra Ahmed Alhammad, Khansa Saleem, Muhammad Ahsan Asghar, Ali Raza, Abd Ullah, Taimoor Hassan Farooq, Jean W. H. Yong, Fei Xu, Mahmoud F. Seleiman, Aamir Riaz
Summary: This study evaluated the effects of cobalt and titanium on the morphological, biochemical, nutritional, and metabolic profile of Pennisetum divisum plants under salinity stress. The results showed that cobalt and titanium treatment could alleviate the increase in methylglyoxal concentration caused by salinity stress, improve antioxidant activity and ion balance, and have a positive impact on the growth and development of P. divisum.
Article
Environmental Sciences
Haifa Abdulaziz Sakit Alhaithloul, Baber Ali, Suliman Mohammed Suliman Alghanem, Faisal Zulfiqar, Sami Asir Al-Robai, Sezai Ercisli, Jean Wan Hong Yong, Anam Moosa, Effa Irfan, Qasim Ali, Muhammad Atif Irshad, Amany H. A. Abeed
Summary: This study investigated the effect of copper oxide nanoparticles (CuO NPs) on cadmium (Cd) accumulation in wheat plants. The results showed that CuO NPs improved the growth and physiology of wheat plants, reduced Cd content, and enhanced the uptake of essential nutrients. This can help reduce the health risks associated with excessive Cd exposure. However, further research is needed to explore different plant species and application methods of CuO NPs.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Environmental Sciences
Sanaullah Jalil, Muhammad Mudassir Nazir, Arwa Abdulkreem AL-Huqail, Baber Ali, Rahmah N. Al-Qthanin, Muhammad A. U. Asad, Mohamed A. Eweda, Faisal Zulfiqar, Nilguen Onursal, Hafiza Ayesha Masood, Jean Wan Hong Yong, Xiaoli Jin
Summary: This study investigated the physiological and molecular responses of rice genotype '9311' to Cd stress and the mitigating effects of silicon oxide nanoparticles (SiO NPs). SiO NPs supplementation showed promise in enhancing Cd tolerance in rice plants while maintaining essential physiological functions.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Review
Green & Sustainable Science & Technology
Annie Drottberger, Yizhi Zhang, Jean Wan Hong Yong, Marie-Claude Dubois
Summary: The environmental impacts of food systems will increase with rapid urban population growth. Alternative solutions, such as urban agriculture, are needed to achieve the UN Sustainable Development Goals. Among these solutions, rooftop farming and rooftop greenhouses offer promising technologies that optimize land use, increase profitability, and reduce energy consumption.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2023)
Article
Environmental Sciences
Md. Nasir Hossain Sani, Mehedi Amin, Abu Bakar Siddique, Saifullah Omar Nasif, Bhim Bahadur Ghaley, Liya Ge, Feng Wang, Jean Wan Hong Yong
Summary: This article explores the potential of waste-derived nano-biochar for sustainable agriculture and environmental applications, including improving soil properties, promoting crop growth, and carbon sequestration and emission reduction. However, more field investigations are needed to assess the potential risks of nano-biochar, such as toxicity.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
