期刊
ELECTROCHIMICA ACTA
卷 130, 期 -, 页码 141-147出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2014.03.014
关键词
Electron transfer; Bioelectrocatalysis; Oxygen reduction; Gold nanoparticle; Laccase
资金
- Swedish Research Council
- Gustaf Th. Ohlsson foundation
- High technology development programme for of Agency for Science, Innovation and Technology, Lithuania
Bioelectrocatalytic reduction of oxygen to water at electrodes modified with gold nanoparticles and a new laccase from Trichaptum abietinum (TaLc) was studied. The bioelectrocatalytic current was found to be much higher at TaLc modified electrodes than at similarly prepared electrodes modified with a broadly used laccase from Trametes hirsuta (ThLc). To explain this difference the bioelectrocatalysis was described in terms of kinetic rate constants based on simultaneous cyclic voltammetry and quartz crystal microbalance measurements. From analysis of the rate constants both laccases appeared to possess similar rates (k(0)) of direct electron transfer. However, the enzyme turnover (k(cat)) was about three-fold higher for gold nanoparticle bound TaLc than for ThLc, calculated using surface concentration of enzyme established by QCM-D. Near reversible potential-induced reorientation of adsorbed proteins was observed by surface enhanced Raman spectroscopy. (C) 2014 Elsevier Ltd. All rights reserved.
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