期刊
ELECTROCHIMICA ACTA
卷 104, 期 -, 页码 178-184出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2013.04.099
关键词
Glucose biosensor; Layer-by-layer assembly; Graphene; Copper phthalocyanine; Noncovalent functionalization
资金
- National Natural Science Foundation of China [21263002, 21161003]
- Guangxi Natural Science Foundation of China [2013GXNSFAA019024, 2013GXNSFGA019001, 2011 GXNSFA018044, 2010GXNSFF013001]
- Foundation Project of Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University), Ministry of Education of China [CMEMR2012-A3, CMEMR2012-A12]
A negatively charged glassy carbon electrode (GCE) was formed through the electrochemical modification of sulfanilic acid (ABS). Subsequently, graphene composites functionalized with copper phthalocyanine-3,4',4 '',4'''-tetrasulfonic acid tetrasodium salt (TSCuPc) or alcian blue pyridine variant (AB) were assembled layer-by-layer (LbL) via alternate electrostatic adsorption onto the ABS/GCE surface to obtain a uniform and stable graphene multilayer film modified electrode. With glucose oxidase (GOD) as an enzyme model, a novel GOD/Nafion/(LbL)(3.5)/ABS/GCE electrochemical biosensor has been developed. SEM and Raman spectra were utilized to characterize the functionalized graphene nanocomposites and modified electrodes. The electrochemical performance of the biosensor was investigated by cyclic voltammetry. The results demonstrated that the graphene multilayer film significantly enhanced the electrocatalytic activity of the modified electrode toward O-2 reduction. Based on the O-2 consumption during the oxidation process of glucose, the as-prepared biosensor exhibited a low detection limit of 0.05 mmol L-1, excellent reproducibility, stability, sensitivity and selectivity, and its response was linear up to 8 mmol L-1 glucose concentration. Accordingly, the multilayer film consisting of copper phthalocyanine functionalized graphene nanocomposites offers a novel and effective platform for the electrochemical biosensing applications. (C) 2013 Elsevier Ltd. All rights reserved.
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