期刊
ELECTROCHIMICA ACTA
卷 55, 期 9, 页码 3206-3212出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2010.01.061
关键词
Alternating current electrophoretic deposition; Saccharomyces cerevisiae; Cell biofilm
资金
- Research Fund KU Leuven [GOA/08/007]
- Belgian Federal Science Policy Office (BELSPO) through the IUAP [P6/17]
In this work, we have explored the possibility of using asymmetrical alternating current electrophoretic deposition (AC-EPD) process, which we have previously reported for enzyme deposition, to immobilize Saccharomyces cerevisiae (S. cerevisiae) cells onto stainless steel substrates. The deposition of S. cerevisiae cells at 30 Hz and 200 V(p-p) permits the formation of 89 +/- 16 mu m thick cell layers in 30 min. The mass of the deposited cells is shown to increase quasi-linearly with the deposition time and the applied amplitude. In order to increase the mechanical stability of the immobilized cells, a thin layer of polyurethane was applied after the AC-EPD of S. cerevisiae cells. The viability of the immobilized cells was tested in the production of ethanol. The results showed that the fermentation process with the immobilized S. cerevisiae cells is more efficient than the fermentation carried out with similarly treated free cells. (C) 2010 Elsevier Ltd. All rights reserved.
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