期刊
ELECTROCHIMICA ACTA
卷 54, 期 23, 页码 5598-5604出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2009.04.064
关键词
Wire beam electrode; Glucose oxidase; Potential/current distribution; Hydrogen peroxide; Biofilm
资金
- National Natural Science Foundation of China (NSFC) [40506019]
The wire beam electrode (WBE) method was first used to study the activity of local glucose oxidase (GOD) on stainless steel surface in seawater. Glucose oxidase was immobilized in calcium alginate gel capsules, which were embedded in a layer of artificial biofilm (calcium alginate gel) on the WBE surface. The potential/current distributions on the WBE surface were mapped using a newly developed device for the WBE method in our lab. The results demonstrated that the catalysis of H(2)O(2) formation by GOD can produce local noble potential peaks and cathodic current zones on the stainless steel surface. An interesting fluctuant current distribution around cathodic zones was observed the first time. The potential and current maps showed that the enzyme heterogeneity of the artificial biofilm caused a corresponding electrochemical heterogeneity at the biofilm/metal interface. The application of the WBE method to ennoblement study enables us to observe the heterogeneous electrochemistry at biofilm/stainless steel interface directly, providing us with a powerful tool to investigate other biofilm-related processes such as microbially influenced corrosion (MIC). (C) 2009 Elsevier Ltd. All rights reserved.
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