Article
Pharmacology & Pharmacy
Fang-Ling Zhao, Qing Zhang, Shuang-Hu Wang, Yun Hong, Shan Zhou, Quan Zhou, Pei-Wu Geng, Qing-Feng Luo, Jie-Fu Yang, Hao Chen, Jian-Ping Cai, Da-Peng Dai
Summary: Cytochrome 2C9 (CYP2C9), an important drug metabolic enzyme in the human hepatic P450 superfamily, has high genetic polymorphism. This study identified four new variants of CYP2C9 in the Chinese Han population, which exhibited reduced enzyme activity compared to the wild-type enzyme.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Marco Lucchino, Anne Billet, Siau-Kun Bai, Estelle Dransart, Justine Hadjerci, Frederic Schmidt, Christian Wunder, Ludger Johannes
Summary: A sensitive and robust assay has been developed for quantifying the relative or absolute numbers of macromolecular drugs in the cytosol, facilitating the optimization of drug delivery vectors and enhancing understanding of the translocation process.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Medicinal
Yong Shi, Deru Meng, Shuanghu Wang, Peiwu Geng, Tao Xu, Quan Zhou, Yunfang Zhou, Wanshu Li, Xugao Chen
Summary: This study found that avitinib has inhibitory effects on CYP450 enzymes, especially on cyp2b1, cyp1a2, and CYP2C9. Therefore, caution should be taken when using avitinib concurrently with drugs metabolized by CYP450 enzymes to avoid potential drug interactions.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Wei Zong, Xiaotong Shao, Yunhe Chai, Xiuwen Wang, Shuang Han, Hongtao Chu, Chuntao Zhu, Xunan Zhang
Summary: An artificial cytosol-contained cell model was constructed using agarose and sucrose, showing increased inhibition efficiency for drug delivery compared to free doxorubicin. The unique release profile of agarose-sucrose@LUVs proved suitable as a drug carrier, demonstrating potential for therapeutic applications.
BIOPHYSICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Kazuya Yokota, Yuki Matsuzawa, Shinji Fukuda, Hideshige Takada, Kaoruko Mizukawa
Summary: Through environmental observations and in vitro experiments, it was found that Cyprinidae have a higher debromination ability, while debromination ability varies widely within Gobiidae. Kinetic parameters show that Cyprinidae fish species have higher vmax values, while vmax values diverge significantly among Gobiidae species.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Estela Villanueva-Badenas, M. Teresa Donato, Laia Tolosa
Summary: Hepatotoxicity or drug-induced liver injury (DILI) is a significant problem in drug development, leading to drug failure and regulatory measures. The development of a co-culture system involving hepatocytes and other cell types has been shown to enhance the understanding and prediction of iDILI. In this study, a co-culture system using HepG2 cells and THP-1-derived macrophages was established and used to evaluate the effects of model iDILI compounds on viability and oxidative stress induction.
Article
Chemistry, Multidisciplinary
Laura Sutarlie, Dave Siak-Wie Ow, Say Kong Ng, Yuansheng Yang, Xiaodi Su
Summary: This study presents a method for quantifying monoclonal antibodies using gold nanoparticles and fluorescently labeled protein G. The method is fast, high throughput, and accurate, suitable for improving mAb production efficiency.
CHEMISTRY-AN ASIAN JOURNAL
(2021)
Article
Medicine, Research & Experimental
Camilo Rey-Bedon, Peony Banik, Aslihan Gokaltun, O. Hofheinz, Martin. L. Yarmush, M. Korkut Uygun, O. Berk Usta
Summary: This study investigates the impact of fatty liver on drug-drug interactions. Dysregulation of enzyme systems observed in fatty liver may increase the risk of unexpected drug interactions. An in vitro model was developed to study enzyme regulation in fatty liver, and it was found that fatty liver might exacerbate drug interactions related to CYP2B6 and CYP2C9 enzymes.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Yixiang Zhong, Jun Sang Yu, Xiaoqiong Wang, Bert Binas, Hye Hyun Yoo
Summary: Our comparison between 5C and 3D PHHs showed that the 5C PHH monolayers perform at least as well as the 3D PHHs in terms of functionality and maintainability, suggesting that the 5C PHH monolayer format combines the advantages of traditional monolayer and spheroid formats.
Article
Oncology
Fabiola Cecchi, Karen Rex, Joanna Schmidt, Cathy D. D. Vocke, Young H. H. Lee, Sandra Burkett, Daniel Baker, Michael A. A. Damore, Angela Coxon, Teresa L. L. Burgess, Donald P. P. Bottaro
Summary: Drug resistance is a major challenge in cancer treatment and it is particularly problematic for targeted therapies that have shown significant successes. In this study, researchers investigated the resistance mechanism to a HGF-neutralizing antibody drug candidate in glioblastoma cells. They found that resistance was acquired through HGF overproduction and misfolding, which led to stress response signaling and redirected transport inside cells. Understanding this mechanism provides insights into drug resistance and suggests strategies for early detection and intervention.
Article
Plant Sciences
Richa Babbar, Barbara Karpinska, Anil Grover, Christine H. Foyer
Summary: Heat stress leads to significant oxidation of both the nuclei and cytosol in plant cells, causing large shifts in glutathione redox potentials. Heat-induced markers such as heat shock protein and ascorbate peroxidase peak after 15 minutes of heat treatment. RNA sequencing analysis reveals significant changes in transcripts encoding heat shock proteins, mitochondrial proteins, transcription factors, and other nuclear localized components upon heat treatment.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Engineering, Biomedical
Zahra Heydari, Ibrahim Zarkesh, Mohammad-Hossein Ghanian, Mahdokht H. Aghdaei, Svetlana Kotova, Ensieh Zahmatkesh, Zahra Farzaneh, Abbas Piryaei, Iman Akbarzadeh, Anastasia Shpichka, Roberto Gramignoli, Peter Timashev, Hossein Baharvand, Massoud Vosough
Summary: Co-culturing primary human hepatocytes with mesenchymal stromal cells and umbilical vein endothelial cells in the presence of liver extracellular matrix-derived microparticles can enhance liver microtissue functionality and drug sensitivity. This approach of tissue-specific microparticle incorporation within a multicellular microtissue may lead to the development of a scalable platform representing multiple functional biofabricated microtissues from different organs in the near future.
BIO-DESIGN AND MANUFACTURING
(2021)
Editorial Material
Physics, Multidisciplinary
Mark Tame
Summary: It has been commonly believed that the quantum statistics of light are maintained when photons interact with plasmons. However, an analysis of the scattering process reveals that light can actually blend different plasmonic pathways, indicating that this assumption is not always true.
Article
Microbiology
Shivani Gargvanshi, William G. Gutheil
Summary: This study screened 1,000 FDA-approved drugs and their metabolites against vancomycin-resistant bacteria and identified potentially synergistic combinations with vancomycin. Several drugs were confirmed to have synergistic effects in follow-up assays.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Adili Keranmu, Li-Bin Pan, Jie Fu, Pei Han, Hang Yu, Zheng-Wei Zhang, Hui Xu, Xin-Yu Yang, Jia-Chun Hu, Hao-Jian Zhang, Meng-Meng Bu, Jian-Dong Jiang, Nian-Zeng Xing, Yan Wang
Summary: The bioavailability of flavonoids is generally low after oral administration, possibly due to the metabolic transformation by gut microbiota. This study identified three possible metabolites of liquiritigenin, an important compound found in Glycyrrhiza uralensis, which may have pharmacological activities. The study compared the metabolic pathways of liquiritigenin by gut microbiota and liver microsomes, providing a theoretical basis for studying pharmacologically active substances in vivo.