Article
Microbiology
Chaoxiong Yue, Chenlu Hu, Peng Xiang, Siming Zhang, Hongde Xiao, Wei Zhou, Hui Jin, Deshi Shi, Jinquan Li, Lang Xu, Yushan Chen, Yan Zeng
Summary: Streptococcus suis (S. suis) is a significant swine and human pathogen that can cause severe meningitis with high mortality. This study demonstrates that autophagy serves as a cellular defense mechanism in microglial cells following S. suis infection, aiding in limiting bacterial invasion and microglial inflammation.
VETERINARY MICROBIOLOGY
(2021)
Article
Microbiology
Shujie Wang, Gang Wang, Yan-Dong Tang, Siqi Li, Lei Qin, Menghang Wang, Yong-Bo Yang, Marcelo Gottschalk, Xuehui Cai
Summary: This study reveals the mechanisms underlying splenic lesions caused by Streptococcus suis infection, including apoptosis and activation of inflammatory responses. The findings provide new insights into the pathogenesis of S. suis.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
Roel M. van Harten, Johanna L. M. Tjeerdsma-van Bokhoven, Astrid de Greeff, Melanie D. Balhuizen, Albert van Dijk, Edwin J. A. Veldhuizen, Henk P. Haagsman, Maaike R. Scheenstra
Summary: The study evaluates the prophylactic capacity of D-CATH-2 and its derivatives in mammalian cells, and finds that they possess strong antibacterial capacity and immunomodulatory function. Administering D-C(1-21) provides partial protection against S. suis infection and reduces disease severity and mortality.
JOURNAL OF ADVANCED RESEARCH
(2022)
Article
Veterinary Sciences
Hongtao Liu, Siyu Lei, Li Jia, Xiaojing Xia, Yingying Sun, Hexiang Jiang, Rining Zhu, Shuguang Li, Guanggang Qu, Jingmin Gu, Changjiang Sun, Xin Feng, Wenyu Han, Paul R. Langford, Liancheng Lei
Summary: This study identifies the binding of SS2 Eno to RPSA on the surface of porcine brain microvascular endothelial cells, leading to activation of signaling pathways that promote intracellular expression of molecules associated with host-cell apoptosis and increased BBB permeability. The findings reveal novel functions for RPSA and HSPD1 in BBB integrity and provide insights for new therapeutic strategies in meningitis.
VETERINARY RESEARCH
(2021)
Article
Microbiology
Xianfeng Hui, Zhongmin Xu, Lei Cao, Liang Liu, Xian Lin, Yong Yang, Xiaomei Sun, Qiang Zhang, Meilin Jin
Summary: The protein HP0487 of Streptococcus suis serotype 2 was found to play a significant role in adhesion and anti-phagocytosis to neutrophils, influencing the pathogenicity and virulence of SS2.
VETERINARY MICROBIOLOGY
(2021)
Article
Genetics & Heredity
Nattinee Kittiwan, Jessica K. Calland, Evangelos Mourkas, Matthew D. Hitchings, Susan Murray, Pakpoom Tadee, Phacharaporn Tadee, Kwanjit Duangsonk, Guillaume Meric, Samuel K. Sheppard, Prapas Patchanee, Ben Pascoe
Summary: This study samples and sequences isolates from healthy pigs in Northern Thailand, revealing extensive genetic diversity and antimicrobial resistance in Streptococcus suis. The presence of antimicrobial resistance genes on integrative and conjugative elements suggests a mobile gene pool that can be accessed by invasive disease isolates.
MICROBIAL GENOMICS
(2022)
Article
Biophysics
Kanjana Kunpatee, Kannika Khantasup, Kittinan Komolpis, Abdulhadee Yakoh, Suphachai Nuanualsuwan, Mohini M. Sain, Sudkate Chaiyo
Summary: This study developed an electrochemical lateral flow immunoassay (eLFIA) strip with high reproducibility for rapid and accurate detection of Streptococcus suis serotype 2. The strip integrated ratiometric electrochemical detection and LFIA, resulting in improved reproducibility compared to a single electrode. The biosensor showed good linearity and a low limit of detection, and was successfully applied in human serum samples.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Immunology
Mei-Fang Tan, Jia Tan, Fan-Fan Zhang, Hai-Qin Li, Hua-Yuan Ji, Shao-Pei Fang, Cheng-Cheng Wu, Yu-Ling Rao, Yan-Bin Zeng, Qun Yang
Summary: This study investigated the pleiotropic effects of exogenous glycogen on Streptococcus suis serotype 2 (SS2) through transcriptome sequencing. The results showed that glycogen induction significantly influenced the metabolic pathways and virulence-associated factors of SS2. Extracellular glycogen utilization enhanced the hemolytic activity, adhesion and invasion ability, and pathogenicity of SS2. These findings are important for understanding the adaptation and pathogenicity mechanisms of SS2.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Lu Wang, Jing Sun, Jiyu Zhao, Jieyu Bai, Yueling Zhang, Yao Zhu, Wanjiang Zhang, Chunlai Wang, Paul R. Langford, Siguo Liu, Gang Li
Summary: In this study, a high-fidelity detection and serotyping platform for Streptococcus suis serotype 2 was developed based on recombinase polymerase amplification (RPA) and a CRISPR-Cas12a system. The platform showed accurate and rapid detection with a detection limit of 10 CFU and the ability to differentiate serotypes. It is a suitable method for point-of-care detection.
Article
Immunology
Alexa N. Lauer, Rene Scholtysik, Andreas Beineke, Christoph Georg Baums, Kristin Klose, Peter Valentin-Weigand, Hiroshi Ishikawa, Horst Schroten, Ludger Klein-Hitpass, Christian Schwerk
Summary: This study utilized RNA-seq to compare global transcriptome profiles of S. suis-infected in vitro cells and in vivo infected pigs, identifying genes involved in inflammatory responses and hypoxia. The results suggest that similar cellular processes occur in infected human and porcine CP epithelial cells, particularly in terms of inflammatory response.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Servane Payen, David Roy, Anais Boa, Masatoshi Okura, Jean-Philippe Auger, Mariela Segura, Marcelo Gottschalk
Summary: The study evaluated the role of Lgt and Lsp enzymes in the pathogenesis of Streptococcus suis serotype 2 infections. Lack of these enzymes led to a significant reduction in S. suis' ability to activate phagocytic cells and induce pro-inflammatory mediators.
Article
Immunology
Quan Li, Xia Fei, Yuhang Zhang, Genglin Guo, Huoying Shi, Wei Zhang
Summary: The study identified a putative virulence-associated factor MutT unique to S. suis serotype 2 virulent strains, essential for the pathogenicity of SS2. MutT knockout mutant significantly decreased bacterial virulence and increased susceptibility to oxidative stress, while enhancing adhesion and invasion to host cells. Deletion of mutT also led to increased microbial clearance in infected host tissues, indicating the crucial biological role of MutT in bacterial pathogenesis during infection.
Article
Veterinary Sciences
Servane Payen, Jesus Aranda Rrodriguez, Mariela Segura, Marcelo Gottschalk
Summary: Streptococcus suis serotype 2 is a significant pathogen in swine, causing economic losses to the industry. The role of the Lmb protein in its pathogenesis was examined and found to have no effect on laminin-binding activity or bacterial adhesion and invasion. However, it was shown to activate cytokines in vitro and play a critical role in zinc acquisition from the host environment.
VETERINARY RESEARCH
(2023)
Article
Multidisciplinary Sciences
Anusak Kerdsin, Dan Takeuchi, Yukihiro Akeda, Shota Nakamura, Marcelo Gottschalk, Kazunori Oishi
Summary: This study compared the genomes of ST1 and ST104 strains of Streptococcus suis and identified 58 unique genomic regions in the ST104 strain, primarily related to metabolism, cell functions, cell wall proteins, bacteriophages, transporters, signal transductions, and lantibiotic proteins. Some virulence genes found in ST1 strains were also present in the ST104 genome. Genomic comparison is a powerful tool for understanding the molecular basis of virulence in S. suis infections.
Article
Biochemical Research Methods
Weiyi Li, Yifan Yin, Yu Meng, Hong Zhou, Zhe Ma, Huixing Lin, Hongjie Fan
Summary: A SILAC comparative quantitative proteomic study revealed 241 upregulated and 81 downregulated differentially expressed proteins in bEnd.3 cells infected with the SS2 ZY05719 or Delta stk strain. These proteins are involved in RNA process, host cytoskeleton, tight junction disruption, and immune response. Some differentially expressed proteins were validated through qPCR and western blot assays, consistent with the quantitative proteomics results.
JOURNAL OF PROTEOMICS
(2021)
Article
Public, Environmental & Occupational Health
V. T. L. Huong, L. V. Thanh, V. D. Phu, D. T. Trinh, K. Inui, N. Tung, N. T. K. Oanh, N. V. Trung, N. T. Hoa, J. E. Bryant, P. W. Horby, N. V. Kinh, H. F. L. Wertheim
EPIDEMIOLOGY AND INFECTION
(2016)
Article
Infectious Diseases
Michael Osterholm, Kristine Moore, Julie Ostrowsky, Kathleen Kimball-Baker, Jeremy Farrar
LANCET INFECTIOUS DISEASES
(2016)
Article
Infectious Diseases
N. V. Cuong, V. N. T. Truc, N. T. Nhung, T. T. Thanh, T. T. B. Chieu, T. Q. Hieu, N. T. Men, H. H. Mai, H. T. Chi, M. F. Boni, H. R. van Doorn, G. E. Thwaites, J. J. Carrique-Mas, N. T. Hoa
TRANSBOUNDARY AND EMERGING DISEASES
(2016)
Article
Public, Environmental & Occupational Health
N. V. Trung, J. J. Carrique-Mas, N. H. Nghia, L. T. P. Tu, H. H. Mai, H. T. Tuyen, J. Campbell, N. T. Nhung, H. N. Nhung, P. V. Minh, T. T. B. Chieu, T. Q. Hieu, N. T. N. Mai, S. Baker, J. A. Wagenaar, N. T. Hoa, C. Schultsz
ZOONOSES AND PUBLIC HEALTH
(2017)
Article
Infectious Diseases
Vu T. L. Huong, Hoang B. Long, Nguyen V. Kinh, Ta T. D. Ngan, Vu T. V. Dung, Behzad Nadjm, H. Rogier van Doorn, Ngo T. Hoa, Peter Horby, Heiman F. L. Wertheim
JOURNAL OF INFECTION
(2018)
Article
Microbiology
Alison E. Mather, Tu Le Thi Phuong, Yunfeng Gao, Simon Clare, Subhankar Mukhopadhyay, David A. Goulding, Nhu Tran Do Hoang, Ha Thanh Tuyen, Nguyen Phu Huong Lan, Corinne N. Thompson, Nguyen Hoang Thu Trang, Juan Carrique-Mas, Ngo Tri Tue, James I. Campbell, Maia A. Rabaa, Duy Pham Thanh, Katherine Harcourt, Ngo Thi Hoa, Nguyen Vinh Trung, Constance Schultsz, Gabriel G. Perron, John E. Coia, Derek J. Brown, Chinyere Okoro, Julian Parkhill, Nicholas R. Thomson, Nguyen Van Vinh Chau, Guy E. Thwaites, Duncan J. Maskell, Gordon Dougan, Linda J. Kenney, Stephen Baker
Article
Multidisciplinary Sciences
Sebastien Matamoros, Jarne M. van Hattem, Maris S. Arcilla, Niels Willemse, Damian C. Melles, John Penders, Trung Nguyen Vinh, Ngo Thi Hoa, Menno D. de Jong, Constance Schultsz
SCIENTIFIC REPORTS
(2017)
Article
Biochemistry & Molecular Biology
John M. Atack, Lucy A. Weinert, Alexander W. Tucker, Asma U. Husna, Thomas M. Wileman, Nazreen F. Hadjirin, Ngo T. Hoa, Julian Parkhill, Duncan J. Maskell, Patrick J. Blackall, Michael P. Jennings
NUCLEIC ACIDS RESEARCH
(2018)
Letter
Medicine, General & Internal
Ngo Thi Hoa, Nguyen Thuy Thuong Thuong, Hannah E. Clapham, Tran Thi Anh Thu, Evelyne Kestelyn, C. Louise Thwaites
Article
Public, Environmental & Occupational Health
Vu Thi Lan Huong, Hugo C. Turner, Nguyen Van Kinh, Pham Quang Thai, Ngo Thi Hoa, Peter Horby, H. Rogier van Doorn, Heiman F. L. Wertheim
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE
(2019)
Correction
Multidisciplinary Sciences
Lucy A. Weinert, Roy R. Chaudhuri, Jinhong Wang, Sarah E. Peters, Jukka Corander, Thibaut Jombart, Abiyad Baig, Kate J. Howell, Minna Vehkala, Niko Valimaki, David Harris, Tran Thi Bich Chieu, Nguyen Van Vinh Chau, James Campbell, Constance Schultsz, Julian Parkhill, Stephen D. Bentley, Paul R. Langford, Andrew N. Rycroft, Brendan W. Wren, Jeremy Farrar, Stephen Baker, Ngo Thi Hoa, Matthew T. G. Holden, Alexander W. Tucker, Duncan J. Maskell, Janine T. Bosse, Yanwen Li, Gareth A. Maglennon, Dominic Matthews, Jon Cuccui, Vanessa Terra
NATURE COMMUNICATIONS
(2019)
Correction
Multidisciplinary Sciences
Sebastien Matamoros, Jarne M. van Hattem, Maris S. Arcilla, Niels Willemse, Damian C. Melles, John Penders, Trung Nguyen Vinh, Ngo Thi Hoa, Martin C. J. Bootsma, Perry J. van Genderen, Abraham Goorhuis, Martin Grobusch, Nicky Molhoek, Astrid M. L. Oude Lashof, Ellen E. Stobberingh, Henri A. Verbrugh, Menno D. de Jong, Constance Schultsz
SCIENTIFIC REPORTS
(2020)
Article
Immunology
Nguyen T. M. Thu, Jasper F. W. Chan, Vo Trieu Ly, Hoa T. Ngo, Ha T. A. Hien, Nguyen P. H. Lan, Nguyen V. V. Chau, Jian-Piao Cai, Patrick C. Y. Woo, Jeremy N. Day, Rogier van Doorn, Guy Thwaites, John Perfect, Kwok Yuen, Thuy Le
Summary: The study assessed the accuracy of Mp1p antigen-detecting enzyme immunoassay in diagnosing talaromycosis, showing high specificity and sensitivity compared to blood culture. Additionally, paired plasma and urine testing significantly increased sensitivity, providing a new tool for rapid diagnosis and potentially reducing mortality rates.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Health Care Sciences & Services
Hung Manh Than, Vuong Minh Nong, Cap Trung Nguyen, Khiem Phu Dong, Hoa Thi Ngo, Tra Thu Doan, Nga Thu Do, Trang Huyen Thi Nguyen, Thanh Van Do, Co Xuan Dao, Tuan Quang Nguyen, Thach Ngoc Pham, Cuong Duy Do
RISK MANAGEMENT AND HEALTHCARE POLICY
(2020)
Article
Immunology
Nguyen Vinh Trung, Sebastien Matamoros, Juan J. Carrique-Mas, Nguyen Huu Nghia, Nguyen Thi Nhung, Tran Thi Bich Chieu, Ho Huynh Mai, Willemien van Rooijen, James Campbell, Jaap A. Wagenaar, Anita Hardon, Nguyen Thi Nhu Mai, Thai Quoc Hieu, Guy Thwaites, Menno D. de Jong, Constance Schultsz, Ngo Thi Hoa
EMERGING INFECTIOUS DISEASES
(2017)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
