Article
Public, Environmental & Occupational Health
Dirk L. Christensen, Theonest K. Mutabingwa, Ib C. Bygbjerg, Allan A. Vaag, Louise G. Grunnet, Fanny Lajeunesse-Trempe, Jannie Nielsen, Christentze Schmiegelow, Kaushik L. Ramaiya, Kathryn H. Myburgh
Summary: This study assessed muscle fibre distribution 20 years after placental and/or peripheral in-utero malaria exposure. The results showed that there were no differences in muscle fibre types or enzymatic activity between the malaria-exposed and non-exposed groups. The findings supported the idea that the mild elevations of plasma glucose levels in people exposed to placental malaria in pregnancy were due to compromised pancreatic insulin secretion rather than insulin resistance.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Biochemistry & Molecular Biology
Bo Song, Yating Cheng, Md. Abul Kalam Azad, Sujuan Ding, Kang Yao, Xiangfeng Kong
Summary: This study investigated the molecular basis for differences in meat yield and quality between Duroc, Taoyuan black (TB), and Xiangcun black (XB) pigs. The results show that TB pigs have higher fat percentage, intramuscular fat content, and antioxidant capacity, but lower carcass weight, lean percentage, pH decline, and glycolytic potential compared to Duroc pigs. Moreover, TB pigs have lower expression of protein synthesis and lipolysis genes in their muscles. Targeted metabolome analysis revealed significant differences in 24 metabolites among the three pig breeds. Correlation analysis suggests that l-malic acid and β-alanine contents in muscles are closely related to meat quality. These findings suggest that the excellent meat quality of TB pigs is attributed to muscle metabolism and fiber characteristics, while lower protein synthesis and lipolysis contribute to less meat yield.
Review
Biochemistry & Molecular Biology
Gabryela Kuhnen, Tiago Guedes Russomanno, Marta Murgia, Nicolas J. Pillon, Martin Schoenfelder, Henning Wackerhage
Summary: Adult skeletal muscle fibres can be classified into different types based on the expression of specific genes. The proportions of these muscle fibre types can be altered through gene gain or loss-of-function. In particular, transcriptional regulators play a significant role in regulating the expression of muscle fibre-specific genes. After exercise, the expression of certain genes increases or decreases, suggesting their involvement in muscle fibre adaptation. DNA sequence variants of muscle fibre genes may contribute to the variation in muscle fibre type proportions in the human population.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Anushree Bharadwaj, Jaydeep Sharma, Jagriti Singh, Mahima Kumari, Tanushri Dargar, Bhargab Kalita, Sam J. Mathew
Summary: Mutations in MYH3 gene cause congenital contracture syndromes, particularly recessive MYH3 mutations lead to spondylocarpotarsal synostosis (SCTS) characterized by vertebral fusions and scoliosis. Knockout of Myh3 in mice showed SCTS phenotypes along with reduced body weight, muscle weight, myofiber size, grip strength, changes in muscle fiber type, altered satellite cell numbers, and increased muscle fibrosis. Activation of YAP signaling pathway was identified as a key contributor to the musculoskeletal defects in Myh3 knockout mice, and inhibiting YAP signaling can rescue these defects.
EMBO MOLECULAR MEDICINE
(2023)
Article
Cell Biology
Masafumi Tanji, Keitaro Wada, Keita Sakamoto, Yudai Ono, Masafumi Inui
Summary: Myosin heavy chains (MyHCs) are the most abundant proteins in myofiber and are encoded by myosin heavy chain (Myh) genes. The expression patterns of Myh genes/MyHC proteins are considered as molecular signatures of specific fiber types or the regenerative status of mammalian skeletal muscles. Immunohistochemistry is costly and not ideal for whole-mount samples, while in situ hybridization (ISH) is not commonly applied for Myh genes due to their highly homologous sequences. However, a new method of ISH analysis using the untranslated region (UTR) sequence of Myh genes has been shown to be cost-effective and specific for analyzing Myh gene expression in whole-mount samples.
DEVELOPMENT GROWTH & DIFFERENTIATION
(2023)
Article
Biochemistry & Molecular Biology
Keisuke Hitachi, Yuri Kiyofuji, Hisateru Yamaguchi, Masashi Nakatani, Masafumi Inui, Kunihiro Tsuchida
Summary: This study investigated the effects of the loss of MyHC-IIb and MyHC-IIx on skeletal muscle function using a mouse model. The results showed that the simultaneous loss of IIb and IIx led to severe muscle hypoplasia after 2 weeks of age in mice. Increased expression of other MyHCs was observed in 3-week-old mice, but it was not sufficient to compensate for the loss of IIb and IIx. Aberrant sarcomere structure and altered expression of sarcomere components were also observed. These findings contribute to the understanding of molecular mechanisms underlying skeletal muscle wasting disorders caused by the loss of skeletal muscle MyHCs.
Article
Biochemistry & Molecular Biology
Pankaj Kumar, Aatifa Zehra, Masum Saini, Sam J. Mathew
Summary: Myh3 gene encodes a skeletal muscle-specific contractile protein, which is crucial for proper myogenic differentiation and function. We identified a promoter-enhancer region that drives Myh3 transcription, and found that Zeb1 and Tle3 proteins are essential trans-factors that differentially regulate Myh3 expression and C2C12 cell myogenic differentiation. Tle3 functions upstream of Zeb1 in regulating myogenic differentiation, and both proteins have post-transcriptional regulatory roles in MyoG expression.
Article
Biochemistry & Molecular Biology
Izumi Matsuba, Rikako Fujita, Kaoruko Iida
Summary: In this study, the effect of palmitic acid (PA), the most common fatty acid component in human fat, on muscle fiber type was assessed, focusing on the expression of fiber-type-specific myosin heavy chain (MHC). The study found that PA selectively suppresses the mRNA and protein expression of fast-twitch MHC, potentially providing a pathogenic mechanism for age-related sarcopenia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Atsuki Fukutani, Shiho Kunimatsu, Tadao Isaka
Summary: This study examined the influence of caffeine on cross-bridge dynamics and found that the effect of caffeine on cross-bridge dynamics is negligible. The ergogenic effect of caffeine is primarily achieved through facilitating Ca2+ release.
SCIENTIFIC REPORTS
(2022)
Article
Endocrinology & Metabolism
Kohei Kido, Tatsuro Egawa, Shinya Watanabe, Kentaro Kawanaka, Jonas T. Treebak, Tatsuya Hayashi
Summary: This study demonstrated the synergistic enhancement of insulin-stimulated TBC1D4 phosphorylation and glucose uptake by fasting and prior muscle contraction, which is associated with augmented AMPK pathway activation.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2022)
Article
Biochemistry & Molecular Biology
Shoji Tanaka, Naotoshi Sugimoto, Takako Ohno-Shosaku, Sachiko Madokoro, Pleiades Tiharu Inaoka, Toshiaki Yamazaki
Summary: This study examined the effects of theobromine on skeletal muscles in rats. The results showed that long-term consumption of a 0.05% theobromine diet had no significant effect on muscle hypertrophy and contractile properties. However, theobromine had similar effects to clenbuterol in upregulating the expression of myosin heavy chain 2x and myogenic differentiation 1.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Cell Biology
Jessica L. Halle, Brittany R. Counts-Franch, Rose M. Prince, James A. Carson
Summary: The study found that in myotubes pre-incubated with C26 CM, the induction of mechanical signaling can still provide a growth stimulus and preserve MyHC-Fast protein expression independent of changes in mRNA expression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Jessica L. Halle, Brittany R. Counts-Franch, Rose M. Prince, James A. Carson
Summary: This study showed that mechanical signaling induced growth stimulus and preserved MyHC-Fast protein expression in myotubes pre-incubated with conditioned media, independent of changes in mRNA expression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Travis Ruan, Dylan Harney, Yen Chin Koay, Lipin Loo, Mark Larance, Leslie Caron
Summary: Skeletal muscle weakness is associated with negative health outcomes. Current research lacks adequate cellular models, but this study developed a scalable monolayer system to differentiate human embryonic stem cells into mature skeletal muscle cells within 26 days. The combination treatment of testosterone, follistatin, and a cocktail of myokines further enhanced muscle cell differentiation and fusion. This cellular model provides a powerful tool for studying muscle development and drug discovery.
Article
Biology
Katharina Hennig, David Hardman, David M. B. Barata, Ines I. B. B. Martins, Miguel O. Bernabeu, Edgar R. Gomes, William Roman
Summary: In this study, a quantitative contractility assay based on optogenetics and particle image velocimetry was designed to induce fiber specification in vitro. Long-term intermittent light-stimulation patterns were applied to cultured myotubes, resulting in enhanced contractile functionality and advanced maturation. This strategy can be used to study fiber specification and refine muscle disease modelling.
LIFE SCIENCE ALLIANCE
(2023)
Article
Endocrinology & Metabolism
Carlos M. Castorena, Edward B. Arias, Naveen Sharma, Jonathan S. Bogan, Gregory D. Cartee
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2015)
Article
Endocrinology & Metabolism
Naveen Sharma, Haiyan Wang, Edward B. Arias, Carlos M. Castorena, Gregory D. Cartee
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2015)
Article
Nutrition & Dietetics
Carlos M. Castorena, Edward B. Arias, Naveen Sharma, Gregory D. Cartee
APPLIED PHYSIOLOGY NUTRITION AND METABOLISM
(2015)
Article
Geriatrics & Gerontology
Yuanyuan Xiao, Naveen Sharma, Edward B. Arias, Carlos M. Castorena, Gregory D. Cartee
Article
Endocrinology & Metabolism
Ognian C. Ikonomov, Diego Sbrissa, Khortnal Delvecchio, Han-Zhong Feng, Gregory D. Cartee, Jian-Ping Jin, Assia Shisheva
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2013)
Article
Endocrinology & Metabolism
Carlos M. Castorena, Edward B. Arias, Naveen Sharma, Gregory D. Cartee
Review
Endocrinology & Metabolism
Gregory D. Cartee
Article
Physiology
George G. Schweitzer, Edward B. Arias, Gregory D. Cartee
JOURNAL OF APPLIED PHYSIOLOGY
(2012)
Article
Biochemistry & Molecular Biology
Michael G. Loeffler, Andreas L. Birkenfeld, Katerina M. Philbrick, Jonathan P. Belman, Estifanos N. Habtemichael, Carmen J. Booth, Carlos M. Castorena, Cheol Soo Choi, Francois R. Jornayvaz, Brandon M. Gassaway, Hui-Young Lee, Gregory D. Cartee, William Philbrick, Gerald I. Shulman, Varman T. Samuel, Jonathan S. Bogan
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Editorial Material
Neurosciences
Gregory D. Cartee
JOURNAL OF PHYSIOLOGY-LONDON
(2014)
Article
Geriatrics & Gerontology
James G. MacKrell, Edward B. Arias, Gregory D. Cartee
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2012)
Article
Geriatrics & Gerontology
Donel A. Sequea, Naveen Sharma, Edward B. Arias, Gregory D. Cartee
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2012)
Article
Cell Biology
Donel A. Sequea, Naveen Sharma, Edward B. Arias, Gregory D. Cartee
MECHANISMS OF AGEING AND DEVELOPMENT
(2013)
Article
Nutrition & Dietetics
Naveen Sharma, Carlos M. Castorena, Gregory D. Cartee
NUTRITION & METABOLISM
(2012)
Article
Multidisciplinary Sciences
Naveen Sharma, Donel A. Sequea, Carlos M. Castorena, Edward B. Arias, Nathan R. Qi, Gregory D. Cartee