Review
Dermatology
Victor Augusti Negri, Fiona M. Watt
Summary: This article discusses the existence of human epidermal stem cells and their clinical application in ex vivo expansion for tissue repair. It also mentions the increasing availability of single-cell RNA-sequencing datasets, which provides new opportunities for studying the nature of epidermal stem cells and testing in vitro experimental models.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Multidisciplinary Sciences
Ryan S. Ziffra, Chang N. Kim, Jayden M. Ross, Amy Wilfert, Tychele N. Turner, Maximilian Haeussler, Alex M. Casella, Pawel F. Przytycki, Kathleen C. Keough, David Shin, Derek Bogdanoff, Anat Kreimer, Katherine S. Pollard, Seth A. Ament, Evan E. Eichler, Nadav Ahituv, Tomasz J. Nowakowski
Summary: During mammalian development, differences in chromatin state play a crucial role in cell fate specification and defining cell identity in the developing brain. Through studying chromatin accessibility patterns, it was found that cerebral organoids recapitulate most cell-type-specific enhancer accessibility patterns but lack some open chromatin regions found in vivo. Systematic comparison of chromatin accessibility across brain regions revealed unexpected diversity among neural progenitor cells and highlighted the role of retinoic acid signaling in neuronal lineage specification in the prefrontal cortex.
Article
Medicine, Research & Experimental
Qingyang Li, Zhenlai Zhu, Lei Wang, Yiting Lin, Hui Fang, Jie Lei, Tianyu Cao, Gang Wang, Erle Dang
Summary: This study utilized single-cell RNA sequencing to investigate the characteristics and heterogeneity of vascular endothelial cells in human dermis, revealing unique features in metabolism, cytokine signaling, chemotaxis, and cell adhesion. Different subtypes of dermal endothelial cells were identified, each showing specific molecular signatures and biological functions. The study demonstrates potential implications for future research on disease-specific alterations of dermal endothelial cells.
Article
Pharmacology & Pharmacy
Minhua Huang, Ning Hua, Siyi Zhuang, Qiuyuan Fang, Jiangming Shang, Zhen Wang, Xiaohua Tao, Jianguo Niu, Xiangyao Li, Peilin Yu, Wei Yang
Summary: This study identified a novel proliferative basal cell (PBC) state that exclusively expresses the transcription factor Cux1 in pathological dry skin. It showed that Cux1 is crucial for keratinocyte proliferation by regulating cyclin-dependent kinases (CDKs) and cyclins. The increased presence of Cux1+ PBCs in patients with psoriasis suggests its importance in epidermal hyperplasia.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Review
Biochemistry & Molecular Biology
Maria Pia Polito, Grazia Marini, Michele Palamenghi, Elena Enzo
Summary: The epidermis is a protective tissue in the human body, consisting of proliferative basal cells and differentiating suprabasal layers. Understanding the molecular mechanisms and pathways involved in keratinocytes' organization and regeneration is crucial for therapeutic approaches. Single-cell techniques have identified disease-specific drivers and therapeutic targets, advancing personalized therapies. This review summarizes the latest findings on transcriptomic and epigenetic profiling of human epidermal cells under different skin conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Chen Weng, Anniya Gu, Shanshan Zhang, Leina Lu, Luxin Ke, Peidong Gao, Xiaoxiao Liu, Yuntong Wang, Peinan Hu, Dylan Plummer, Elise Macdonald, Saixian Zhang, Jiajia Xi, Sisi Lai, Konstantin Leskov, Kyle Yuan, Fulai Jin, Yan Li
Summary: This study identifies type II diabetes-associated pancreatic ss-cell heterogeneity at both transcriptomic and epigenomic levels through comprehensive analysis of single-cell transcriptome, single-nuclei chromatin accessibility, and cell-type specific 3Dgenome profiles. The researchers develop a computational method to dissect the intra-donor and inter-donor heterogeneity between single ss-cells, revealing distinct mechanisms of type II diabetes pathogenesis. Integrative transcriptomic and epigenomic analysis uncovers HNF1A as a principal driver of intra-donor heterogeneity and reduced expression in ss-cells from type II diabetes donors.
NATURE COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Ankush Sharma, Akshay Akshay, Marie Rogne, Ragnhild Eskeland
Summary: The developed ShinyArchR.UiO application facilitates better understanding of gene regulatory mechanisms and sharing of massive chromatin accessibility data, improving data accessibility. Additionally, it also streamlines collaborative efforts among researchers.
Article
Oncology
Zhenyuan Yu, Yufang Lv, Cheng Su, Wenhao Lu, RuiRui Zhang, Jiaping Li, Bingqian Guo, Haibiao Yan, Deyun Liu, Zhanbin Yang, Hua Mi, Linjian Mo, Yi Guo, Wenyu Feng, Haotian Xu, Wenyi Peng, Jiwen Cheng, Aruo Nan, Zengnan Mo
Summary: This study performed single-cell RNA sequencing (scRNA-seq) and single-cell assay for transposase-accessible chromatin using sequencing (scATAC-seq) on 19 ccRCC samples to construct single-cell transcriptome and chromatin accessibility maps. It revealed the regulatory characteristics of different tumor cell subtypes in ccRCC and identified two long noncoding RNAs that promote ccRCC invasion and migration. This comprehensive analysis provides new insights into the biology and treatment of ccRCC.
Article
Genetics & Heredity
Caleb A. Lareau, Sonia M. Dubois, Frank A. Buquicchio, Yu-Hsin Hsieh, Kopal Garg, Pauline Kautz, Lena Nitsch, Samantha D. Praktiknjo, Patrick Maschmeyer, Jeffrey M. Verboon, Jacob C. Gutierrez, Yajie Yin, Evgenij Fiskin, Wendy Luo, Eleni P. Mimitou, Christoph Muus, Rhea Malhotra, Sumit Parikh, Mark D. Fleming, Lena Oevermann, Johannes Schulte, Cornelia Eckert, Anshul Kundaje, Peter Smibert, Santosha A. Vardhana, Ansuman T. Satpathy, Aviv Regev, Vijay G. Sankaran, Suneet Agarwal, Leif S. Ludwig
Summary: This study establishes a multi-omics approach to quantify deletions in mtDNA in single cells and reveals the dynamics of pathogenic mtDNA deletion heteroplasmy consistent with purifying selection and distinct metabolic vulnerabilities across T-cell states.
Article
Allergy
Natalia Alkon, Wolfgang M. Bauer, Thomas Krausgruber, Issac Goh, Johannes Griss, Vy Nguyen, Baerbel Reininger, Christine Bangert, Clement Staud, Patrick M. Brunner, Christoph Bock, Muzlifah Haniffa, Georg Stingl
Summary: This study investigates the phenotypic and molecular features of cutaneous innate lymphoid cells (ILCs) to better understand the biological role of these cells. The findings demonstrate the flexibility of the skin immune system, with ILCs in diseased skin expressing genes characteristic of different immune types. This suggests that the skin immune system can adjust its function to adapt to the environment.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Jing Wang, Cai Zhou, Shuai Gao, Xiuling Song, Xinyan Yang, Jiaqi Fan, Shaofang Ren, Linzi Ma, Jiexiang Zhao, Manman Cui, Ke Song, Mei Wang, Chaohui Li, Yi Zheng, Fang Luo, Kai Miao, Xiaochun Bai, Andrew P. Hutchins, Lin Li, Gang Chang, Xiao-Yang Zhao
Summary: Round spermatid injection (ROSI) technique shows promise for treating infertility in some men. However, the developmental potential of ROSI embryos is compromised and the mechanisms are not fully understood. This study explores the transcriptome, chromatin accessibility, and DNA methylation patterns of ROSI embryos derived from early-stage round spermatids using single-cell multiomics sequencing. It identifies reprogramming defects in ROSI embryos at the pronuclear stages, primarily associated with misexpression of a group of minor zygotic genome activation genes. The study also discovers a small compound, A366, that can improve the developmental potential of ROSI embryos by correcting epigenetic and transcriptomic states.
Article
Biotechnology & Applied Microbiology
Kunal Jindal, Mohd Tayyab Adil, Naoto Yamaguchi, Xue Yang, Helen C. Wang, Kenji Kamimoto, Guillermo C. Rivera-Gonzalez, Samantha A. Morris
Summary: This study presents a new method called CellTag-multi, which allows the direct capture of heritable random barcodes expressed as polyadenylated transcripts in single-cell RNA sequencing and single-cell Assay for Transposase Accessible Chromatin using sequencing assays. The CellTag-multi method enables independent clonal tracking of transcriptional and epigenomic cell states and has been validated in studying progenitor cell lineage priming and reprogramming processes. The findings demonstrate the utility of CellTag-multi in revealing gene regulatory changes and providing mechanistic insights into cell fate.
NATURE BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xuyang Shi, Wen Ma, Shanshan Duan, Quan Shi, Shuo Wu, Shijie Hao, Guoyi Dong, Jinxiu Li, Yumo Song, Chang Liu, Xiumei Lin, Yue Yuan, Qiuting Deng, Jiangshan Xu, Shiyi Bai, Yong Hou, Chuanyu Liu, Longqi Liu
Summary: A comprehensive analysis using single-cell RNA sequencing and single-cell sequencing assay for transposase-accessible chromatin was conducted to profile neonatal umbilical cord blood cells and adult peripheral blood mononuclear cells and identify their composition and differentially expressed genes. Key genes were found to be expressed in the immune cells of neonatal umbilical cord blood, while high cytotoxic gene expression was observed in natural killer and T cells from adult peripheral blood. The results provide valuable insights into the regulatory mechanisms of different immune cell types and the potential underlying mechanisms for neonatal immune tolerance.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Ryan M. Mulqueen, Dmitry Pokholok, Brendan L. O'Connell, Casey A. Thornton, Fan Zhang, Brian J. O'Roak, Jason Link, Galip Gurkan Yardimci, Rosalie C. Sears, Frank J. Steemers, Andrew C. Adey
Summary: The s3 technology, using a uracil-based adapter switching approach, significantly improves the usable reads per cell in single-cell genomics assays, especially in chromatin accessibility analysis. Compared with other methods, s3 demonstrates higher efficiency and resolution in single-cell whole-genome sequencing and chromatin conformation analysis.
NATURE BIOTECHNOLOGY
(2021)
Article
Immunology
Fabio F. Rosa, Cristiana F. Pires, Ilia Kurochkin, Evelyn Halitzki, Tasnim Zahan, Nejc Arh, Olga Zimmermannova, Alexandra G. Ferreira, Hongzhe Li, Stefan Karlsson, Stefan Scheding, Carlos-Filipe Pereira
Summary: Researchers have identified key gene regulatory networks driving the differentiation and reprogramming of human cDC1 cells, improving the efficiency of reprogramming and providing a new platform for generating patient-specific cDC1 cells for cancer immunotherapy vaccination strategies.
SCIENCE IMMUNOLOGY
(2022)
Article
Cell Biology
Jaimee E. Hoefert, Glen A. Bjerke, Dongmei Wang, Rui Yi
JOURNAL OF CELL BIOLOGY
(2018)
Article
Developmental Biology
Sarah J. Jackson, Zhaojie Zhang, Dejiang Feng, Meaghan Flagg, Evan O'Loughlin, Dongmei Wang, Nicole Stokes, Elaine Fuchs, Rui Yi
Article
Cell Biology
Dongmei Wang, Zhaojie Zhang, Evan O'Loughlin, Thomas Lee, Stephane Houel, Donal O'Carroll, Alexander Tarakhovsky, Natalie G. Ahn, Rui Yi
GENES & DEVELOPMENT
(2012)
Article
Oncology
Dongmei Wang, Zhenwen Zhao, Andrea Caperell-Grant, Gong Yang, Samuel C. Mok, Jinsong Liu, Robert M. Bigsby, Yan Xu
MOLECULAR CANCER THERAPEUTICS
(2008)
Article
Cell Biology
Dongmei Wang, Zhaojie Zhang, Evan O'Loughlin, Li Wang, Xiying Fan, Eric C. Lai, Rui Yi
NATURE CELL BIOLOGY
(2013)
Editorial Material
Cell Biology
Dongmei Wang, Rui Yi
GENES & DEVELOPMENT
(2019)
Article
Biochemistry & Molecular Biology
D Wang, H Li, H Yuan, M Zheng, C Bai, L Chen, X Pei