Article
Developmental Biology
Jessica M. Kraus, Dion Giovannone, Renata Rydzik, Jeremy L. Balsbaugh, Isaac L. Moss, Jennifer L. Schwedler, Julien Y. Bertrand, David Traver, Kurt D. Hankenson, J. Gage Crump, Daniel W. Youngstrom
Summary: This study shows that Notch signaling is induced during the early stages of bone healing and its modulation can affect the size and quality of bone regeneration. The findings suggest that targeting Notch signaling may provide a new strategy to enhance bone healing in mammals.
Article
Biochemistry & Molecular Biology
Hung-Yu Shih, Chia-Wei Chang, Yi-Chieh Chen, Yi-Chuan Cheng
Summary: Bone morphogenetic protein (BMP) signaling plays a crucial role in the regulation of neural induction, neuronal specification, and neuronal differentiation. However, its role in neural progenitors is still not well understood. By manipulating BMP signaling in zebrafish embryos, researchers discovered that inhibiting BMP signaling during gastrulation can increase the proliferation of neural progenitors and decrease the number of neuronal precursors. This study also revealed that there is a fine balance between BMP and Notch signaling in regulating the proliferation of neural progenitor cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Chao Liu, Zhenhao Ying, Zifa Li, Long Zhang, Xin Li, Wenbo Gong, Jiang Sun, Xuejing Fan, Ke Yang, Xingchen Wang, Sheng Wei, Ning Dong
Summary: This study confirmed the association between GAD and abnormal expression of the Notch signaling pathway, and demonstrated that DZXYS can promote hippocampal neuronal regeneration by regulating the Notch signaling pathway to alleviate anxiety behavior.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Developmental Biology
Yukino Wada, Hikaru Tsukatani, Chihiro Kuroda, Yurika Miyazaki, Miku Otoshi, Isao Kobayashi
Summary: This study identifies a previously unknown spatiotemporal regulatory mechanism of hematopoietic stem cell specification by somites in zebrafish, in which Jag2b-induced Notch signaling leads to the expression of wnt16 and HSC markers.
Article
Toxicology
Dou Yang, Juhua Xiao, Mengqi Wan, Jieping Liu, Ling Huang, Xue Li, Li Zhang, Fasheng Liu, Desheng Liang, Yongliang Zheng, Baogang Xie, Xinjun Liao, Guanghua Xiong, Huiqiang Lu, Zigang Cao, Shouhua Zhang
Summary: This study investigated the effects of roxadustat on early embryonic development using zebrafish. The results showed that roxadustat exposure caused abnormal embryonic development and liver metabolic imbalance in zebrafish embryos. Additionally, roxadustat down-regulated Notch signaling, which contributed to hepatotoxicity. This research provides valuable insights for the clinical use of roxadustat.
JOURNAL OF APPLIED TOXICOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Chia-Chen Hsu, Andrea Serio, Sahana Gopal, Amy Gelmi, Ciro Chiappini, Ravi A. Desai, Molly M. Stevens
Summary: Surface topography, such as microgrooves, can alter the behavior and differentiation patterns of stem cells by enhancing alignment, modifying nuclear geometry, increasing cellular stiffness, and inducing epigenetic changes. In this study, microgrooves were found to promote neuronal differentiation of neural stem cells by downregulating Notch signaling and modifying the epigenetic landscape.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Yanjun Zong, Yuanyao Chen, Yongfeng Wang, Jingming Wang, Zhiquan Yu, Zixuan Ou, Jinyu Chen, Huiping Zhang, Chunyan Liu
Summary: This study reveals the cardiotoxicity of p,p'-DDE in zebrafish embryos and suggests its association with the JAK-STAT and Notch signaling pathways.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Review
Cell Biology
Frederick Allen, Ivan Maillard
Summary: The Notch signaling pathway has been investigated as a therapeutic target for cancers and immune/inflammatory disorders, orchestrating cell fate decisions and regulating immune responses through cell-to-cell contacts. It has shown potential in inhibiting tumor growth and preventing/reversing inflammatory disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Meimei Lin, Hengfen Dai, Lanlan Zheng, Peiqing Yu, Yan Chen, Ru Hong, Caiyun Zheng
Summary: This study found that Tryptanthrin can inhibit tumor growth by down-regulating Dll4 protein expression and up-regulating the expression of desma and Tnnt2c genes.
TRANSLATIONAL CANCER RESEARCH
(2023)
Article
Biology
Tanya L. Brown, Emma C. Horton, Evan W. Craig, Camille E. A. Goo, Erik C. Black, Madeleine N. Hewitt, Nathaniel G. Yee, Everett T. Fan, David W. Raible, Jeffrey P. Rasmussen, Marianne E. Bronner
Summary: This study discovered Merkel cells in zebrafish epidermis and identified Atoh1a as a marker for zebrafish Merkel cells. Merkel cells in zebrafish develop from basal keratinocytes and exhibit neurosecretory and mechanosensory machinery, actin-rich microvilli, and complex with somatosensory axons, similar to mammalian Merkel cells. The study also found region-specific densities and distribution patterns of Merkel cells in different skin compartments.
Article
Developmental Biology
Oren Gozlan, David Sprinzak
Summary: Notch signaling is a highly conserved pathway that plays a crucial role in the development and homeostasis of various organisms. It is activated by direct cell-cell contact and mechanical pulling of receptors. Notch signaling coordinates the differentiation into distinct cell fates of neighboring cells during development, and its activation is regulated at multiple levels. This article provides an overview of the activation mechanism and regulation of the Notch pathway, as well as its importance in various developmental processes.
Article
Multidisciplinary Sciences
Lei Liu, Tianyu Tao, Shihua Liu, Xia Yang, Xuwei Chen, Jiaer Liang, Ruohui Hong, Wenting Wang, Yi Yang, Xiaoyi Li, Youhong Zhang, Quanfeng Li, Shujun Liang, Haocheng Yu, Yun Wu, Xinyu Guo, Yan Lai, Xiaofan Ding, Hongyu Guan, Jueheng Wu, Xun Zhu, Jie Yuan, Jun Li, Shicheng Su, Mengfeng Li, Xiuyu Cai, Junchao Cai, Han Tian
Summary: The study reveals that the positive feedback loop between RFC4 and NICD1 plays a critical role in linking NSCLC metastasis and stemness properties, with potential therapeutic and diagnostic/prognostic implications for NSCLC therapy.
NATURE COMMUNICATIONS
(2021)
Article
Gastroenterology & Hepatology
Seung-Hoon Lee, Juhoon So, Donghun Shin
Summary: This study established a zebrafish model for the conversion of hepatocytes to biliary epithelial cells (BECs). The conversion process involves sequential induction of BEC markers and disappearance of hepatocyte markers. Live time-lapse imaging revealed the transdifferentiation of hepatocytes into BECs without an intermediate proliferative state.
Article
Pharmacology & Pharmacy
Chao Chen, Yongliang Zheng, Xue Li, Li Zhang, Kangyu Liu, Sujie Sun, Zilin Zhong, Hongmei Hu, Fasheng Liu, Guanghua Xiong, Xinjun Liao, Huiqiang Lu, Yanlong Bi, Jianjun Chen, Zigang Cao
Summary: In this study, a zebrafish model was used to evaluate the effects of cysteamine on skeletal development. The results showed that cysteamine caused skeletal defects, reduced locomotion activity, and increased oxidative stress in the skeleton. Additionally, cysteamine affected the expression of Notch signaling and disrupted notochordal cells. These findings suggest that cysteamine has potential skeletal toxicity and the underlying mechanisms involve the inhibition of Notch signaling and disruption of notochordal cells.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Neurosciences
Takashi Shimazaki, Masashi Tanimoto, Yoichi Oda, Shin-ichi Higashijima
JOURNAL OF NEUROSCIENCE
(2019)
Article
Multidisciplinary Sciences
Yukiko Kimura, Shin-ichi Higashijima
NATURE COMMUNICATIONS
(2019)
Article
Neurosciences
Thomas Frank, Nila R. Monig, Chie Satou, Shin-ichi Higashijima, Rainer W. Friedrich
NATURE NEUROSCIENCE
(2019)
Article
Neurosciences
Yuto Uemura, Kagayaki Kato, Koichi Kawakami, Yukiko Kimura, Yoichi Oda, Shin-ichi Higashijima
JOURNAL OF NEUROSCIENCE
(2020)
Article
Developmental Biology
Takamasa Mizoguchi, Michi Fukada, Miku Iihama, Xuehui Song, Shun Fukagawa, Shuhei Kuwabara, Shuhei Omaru, Shin-ichi Higashijima, Motoyuki Itoh
Article
Neurosciences
Zhikai Liu, Yukiko Kimura, Shin-ichi Higashijima, David G. C. Hildebrand, Joshua L. Morgan, Martha W. Bagnall
Article
Chemistry, Medicinal
Siripat Chaichit, Takuto Sato, Huiqing Yu, Yu-ki Tanaka, Yasumitsu Ogra, Takamasa Mizoguchi, Motoyuki Itoh
Summary: A rapid dexamethasone-induced osteoporosis animal model using zebrafish scales was demonstrated, along with a method to measure the activities of bone-related enzymes. Dexamethasone treatment increased osteoclast activity but did not significantly affect the activities of TRAP and ALP.
Article
Neurosciences
Urs L. Bohm, Yukiko Kimura, Takashi Kawashima, Misha B. Ahrens, Shin-ichi Higashijima, Florian Engert, Adam E. Cohen
Summary: The activity of ventral V3 neurons in zebrafish is positively correlated with swimming strength and bout length. Activation of these neurons leads to stronger and longer swimming, but does not affect tail beat frequency. Genetic ablation experiments further confirm the critical role of V3 neurons in locomotor adaptation.
Article
Biology
Chie Satou, Rachael L. Neve, Hassana K. Oyibo, Pawel Zmarz, Kuo-Hua Huang, Estelle Arn Bouldoires, Takuma Mori, Shin-ichi Higashijima, Georg B. Keller, Rainer W. Friedrich
Summary: This study developed methods for gene transfer and retrograde tracing in zebrafish, as well as efficient transneuronal tracing. Herpes simplex viruses and modified rabies viruses were used to visualize and manipulate neurons in zebrafish.
Article
Multidisciplinary Sciences
Masashi Tanimoto, Ikuko Watakabe, Shin-Ichi Higashijima
Summary: Spatio-temporal information about head orientation and movement is crucial for balance and motion perception. In this study, we used in vivo Ca2+ imaging to investigate the response patterns of hair cells and ganglion neurons in larval zebrafish to static tilt and vibration. Our results suggest that different types of hair cells in the otolith organs respond selectively to head vibration and static tilt, while maintaining a spatially ordered direction preference that is preserved in ganglion neurons. This demonstrates the presence of topographically organized selectivity for the direction and dynamics of head orientation/movement in the vestibular periphery.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Takamasa Mizoguchi, Shohei Mikami, Mari Yatou, Yui Kondo, Shuhei Omaru, Shuhei Kuwabara, Wataru Okura, Syouta Noda, Takeshi Tenno, Hidekazu Hiroaki, Motoyuki Itoh
Summary: Two small molecules, NPL1010 and NPL3008, have been found to modulate BMP signaling and affect dorsal-ventral patterning and bone formation in zebrafish embryos. They bind to BMP1 and selectively inhibit Chordin cleavage, thereby acting as valuable inhibitors of BMP signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Takumi Sugioka, Masashi Tanimoto, Shin-ichi Higashijima
Summary: This study demonstrates that larval zebrafish can finely control their posture through a body bend reflex. The authors also identify the neural circuits and muscles involved in this reflex.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Tohgo Kanoh, Jingyu Lu, Takamasa Mizoguchi, Motoyuki Itoh
Summary: Breast cancer, a common and invasive cancer among women, is often difficult to treat due to metastasis. This study reveals the relationship between breast cancer cell migration and the E3 ubiquitin ligase Mind bomb1 (MIB1). The downregulation of MIB1 promotes cell migration and impairs E-cadherin membrane localization in breast cancer cells, suggesting a potential role of MIB1 in suppressing breast cancer metastasis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Neurosciences
Irene Pallucchi, Maria Bertuzzi, David Madrid, Pierre Fontanel, Shin-ichi Higashijima, Abdeljabbar El Manira
Summary: This study links the molecular diversity of motoneurons and V2a interneurons with the modular circuit organization that controls locomotor speed in adult zebrafish. It reveals functional segregation and shared molecular signatures among different subtypes of neurons, providing important insights into the molecular mechanisms underlying motor circuit modularity.
NATURE NEUROSCIENCE
(2023)
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)
