Article
Developmental Biology
Kayt Scott, Rebecca O'Rourke, Caitlin C. Winkler, Christina A. Kearns, Bruce Appel
Summary: Single-cell RNA sequencing of pMN cells in embryonic zebrafish trunk tissue revealed two distinct progenitor populations: one generating neurons and the other generating OPCs. A subpopulation called Pre-OPCs, marked by the expression of the homeobox transcription factor gene gsx2, was identified in the spinal cord prior to canonical OPC marker gene expression.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Eveljn Scarian, Matteo Bordoni, Valentina Fantini, Emanuela Jacchetti, Manuela Teresa Raimondi, Luca Diamanti, Stephana Carelli, Cristina Cereda, Orietta Pansarasa
Summary: This study demonstrates the robustness of 3D cultures for the differentiation of stem cells in studying ALS, using bioprinting and iPSCs. The results show that the hydrogel-based constructs do not interfere with the differentiation process and electrophysiological features of the cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Cathy Danesin, Romain Darche-Gabinaud, Nathalie Escalas, Vanessa Bouguetoch, Philippe Cochard, Amir Al Oustah, David Ohayon, Bruno Glise, Cathy Soula
Summary: Sulf2a, as a member of the extracellular sulfatases Sulf family, plays a crucial role in regulating the cell type specification during spinal cord development by modulating Sonic Hedgehog signaling pathway. Depletion of Sulf2a leads to overproduction of V3 interneurons at the expense of motor neurons and impedes generation of oligodendrocyte precursor cells. Thus, Sulf2a acts by maintaining correct patterning and specification of ventral progenitors, ensuring the proper production of motor neurons and OPCs dependent on Shh signaling.
SCIENTIFIC REPORTS
(2021)
Article
Developmental Biology
Matthew Wind, Antigoni Gogolou, Ichcha Manipur, Ilaria Granata, Larissa Butler, Peter W. Andrews, Ivana Barbaric, Ke Ning, Mario R. Guarracino, Marysia Placzek, Anestis Tsakiridis
Summary: This study identified the signals guiding the transition of human NMP-like cells towards thoracic ventral spinal cord neurectoderm and defined an optimized protocol for generating thoracic MNs, facilitating the comparison of hPSC-derived spinal cord cells of distinct axial identities.
Article
Neurosciences
Michael Jay, Malcolm A. MacIver, David L. McLean
Summary: This study investigates how spinal circuits contribute to direction control during ongoing locomotion in larval zebrafish. The results show that directed swimming involves changes in motor output duration and increased recruitment of motor neurons, without affecting the timing of spiking along the body. The study also reveals different recruitment patterns in excitatory and inhibitory interneurons, supporting the modular control of steering and propulsion by spinal premotor circuits.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Multidisciplinary Sciences
Curtis A. Benson, Kai-Lan Olson, Siraj Patwa, Marike L. Reimer, Lakshmi Bangalore, Myriam Hill, Stephen G. Waxman, Andrew M. Tan
Summary: Research using viral intervention techniques in mice after spinal cord injury found that knocking out Rac1 protein can restore dendritic spine development and reduce H-reflex excitability, thereby reversing spasticity. This indicates a potential mechanistic relationship between abnormal motor neuron dendritic spines and spasticity induced by SCI.
SCIENTIFIC REPORTS
(2021)
Article
Neurosciences
Latoya L. Allen, Nicole L. Nichols, Zachary A. Asa, Anna T. Emery, Marissa C. Ciesla, Juliet V. Santiago, Ashley E. Holland, Gordon S. Mitchell, Elisa J. Gonzalez-Rothi
Summary: The study tested the hypothesis that C2 spinal hemisection (C2Hx) minimally impacts phrenic motor neuron (PMN) survival and found no evidence of PMN loss at 2- or 8-weeks post-C2Hx. This insight is crucial for studying strategies for inducing plasticity and/or regeneration within the phrenic motor system after high cervical injury.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Biology
Saul Bello-Rojas, Martha W. Bagnall
Summary: The study found that sister V2a/b neurons in the zebrafish spinal cord receive input from distinct presynaptic sources, do not communicate with each other, and connect to largely distinct targets.
Article
Chemistry, Multidisciplinary
Jessica Aijia Liu, Kin Wai Tam, Yong Long Chen, Xianglan Feng, Christy Wing Lam Chan, Amos Lok Hang Lo, Kenneth Lap-Kei Wu, Man-Ning Hui, Ming-Hoi Wu, Ken Kwok-Keung Chan, May Pui Lai Cheung, Chi Wai Cheung, Daisy Kwok-Yan Shum, Ying-Shing Chan, Martin Cheung
Summary: The study found that neural stem cells derived from human pluripotent stem cells have therapeutic potential for spinal cord injury. By reducing the expression of the SOX9 gene, the neurogenic capacity of the transplanted cells is enhanced, leading to improved integration and functional recovery.
Article
Cell Biology
Jianjian Zhu, Rashmi Patel, Anna Trofka, Brian D. Harfe, Susan Mackem
Summary: This study demonstrates that a transient, early pulse of Sonic hedgehog (Shh) is necessary and sufficient for normal mouse limb development, bypassing the requirement for cell survival. Shh response is short range and limited to a specific time window and region, and it patterns digit identities indirectly.
DEVELOPMENTAL CELL
(2022)
Article
Chemistry, Analytical
Zheng Ao, Hongwei Cai, Zhuhao Wu, Jonathan Krzesniak, Chunhui Tian, Yvonne Y. Lai, Ken Mackie, Feng Guo
Summary: The study introduces a human spinal organoid-on-a-chip device for modeling the biology and electrophysiology of human nociceptive neurons and dorsal horn interneurons. The device allows for testing nociceptive modulators and is cost-efficient, scalable, and easy to use. By integrating human sensory-spinal-cord organoids, the method shows promise for screening and validating novel therapeutics for human pain medicine discovery.
ANALYTICAL CHEMISTRY
(2022)
Review
Neurosciences
Marissa C. Ciesla, Yasin B. Seven, Latoya L. Allen, Kristin N. Smith, Zachary A. Asa, Alec K. Simon, Ashley E. Holland, Juliet Santiago, Kelsey Stefan, Ashley Ross, Elisa J. Gonzalez-Rothi, Gordon S. Mitchell
Summary: The study found that there is substantial serotonergic reinnervation following cervical spinal cord injury, but intermittent hypoxia does not affect the extent of reinnervation.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Pharmacology & Pharmacy
Qiong Zhang, Mingfu Liu, Haibin Nong, Yanan Zhang, Yiguang Bai, Pan Liu, Shaohui Zong, Gaofeng Zeng
Summary: TFHL has shown to improve motor function recovery and promote spinal cord tissue repair in rats with spinal cord injury (SCI). It reduces apoptosis and improves the functional status of neurons. TFHL also improves cell morphology, microstructure, and axonal regeneration of damaged spinal motor neurons, and promotes the secretion of neurotrophic factors. Furthermore, TFHL promotes autophagy and related protein expression both in vivo and in vitro.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Medicine, Research & Experimental
Mohammed R. Shaker, Ju-Hyun Lee, Kyung Hyun Kim, Saeli Ban, Veronica Jihyun Kim, Joo Yeon Kim, Ji Yeoun Lee, Woong Sun
Summary: NMP progenitors initially located at the tail tip in mouse development, later found in the caudal neural tube, gradually contributing to the spinal cord during axial elongation. The derived NPCs preferentially contributed to the ventral side at the lumbar spinal cord level first, followed by the dorsal side.
Article
Biology
Evdokia Menelaou, Sandeep Kishore, David L. McLean, Markus Meister
Summary: This study unravels the impact of mixed synapses within the spinal locomotor circuitry of larval zebrafish and finds that differences in mixed synapse density can contribute to excitability underestimations and connectivity overestimations, explaining the "violations" of the size principle.
Article
Neurosciences
Xi Huang, Ye He, Adrian M. Dubuc, Rintaro Hashizume, Wei Zhang, Jueri Reimand, Huanghe Yang, Tongfei A. Wang, Samantha J. Stehbens, Susan Younger, Suzanne Barshow, Sijun Zhu, Michael K. Cooper, John Peacock, Vijay Ramaswamy, Livia Garzia, Xiaochong Wu, Marc Remke, Craig M. Forester, Charles C. Kim, William A. Weiss, C. David James, Marc A. Shuman, Gary D. Bader, Sabine Mueller, Michael D. Taylor, Yuh Nung Jan, Lily Yeh Jan
NATURE NEUROSCIENCE
(2015)
Article
Neurosciences
Yuanquan Song, David Sretavan, Ernesto A. Salegio, Jim Berg, Xi Huang, Tong Cheng, Xin Xiong, Shan Meltzer, Chun Han, Trong-Tuong Nguyen, Jacqueline C. Bresnahan, Michael S. Beattie, Lily Yeh Jan, Yuh Nung Jan
NATURE NEUROSCIENCE
(2015)
Article
Oncology
Sameer Agnihotri, Brian Golbourn, Xi Huang, Marc Remke, Susan Younger, Rob A. Cairns, Alan Chalil, Christian A. Smith, Stacey-Lynn Krumholtz, Danielle Mackenzie, Patricia Rakopoulos, Vijay Ramaswamy, Michael S. Taccone, Paul S. Mischel, Gregory N. Fuller, Cynthia Hawkins, William L. Stanford, Michael D. Taylor, Gelareh Zadeh, James T. Rutka
Article
Multidisciplinary Sciences
A. Sorana Morrissy, Livia Garzia, David J. H. Shih, Scott Zuyderduyn, Xi Huang, Patryk Skowron, Marc Remke, Florence M. G. Cavalli, Vijay Ramaswamy, Patricia E. Lindsay, Salomeh Jelveh, Laura K. Donovan, Xin Wang, Betty Luu, Kory Zayne, Yisu Li, Chelsea Mayoh, Nina Thiessen, Eloi Mercier, Karen L. Mungall, Yusanne Ma, Kane Tse, Thomas Zeng, Karey Shumansky, Andrew J. L. Roth, Sohrab Shah, Hamza Farooq, Noriyuki Kijima, Borja L. Holgado, John J. Y. Lee, Stuart Matan-Lithwick, Jessica Liu, Stephen C. Mack, Alex Manno, K. A. Michealraj, Carolina Nor, John Peacock, Lei Qin, Juri Reimand, Adi Rolider, Yuan Y. Thompson, Xiaochong Wu, Trevor Pugh, Adrian Ally, Mikhail Bilenky, Yaron S. N. Butterfield, Rebecca Carlsen, Young Cheng, Eric Chuah, Richard D. Corbett, Noreen Dhalla, An He, Darlene Lee, Haiyan I. Li, William Long, Michael Mayo, Patrick Plettner, Jenny Q. Qian, Jacqueline E. Schein, Angela Tam, Tina Wong, Inanc Birol, Yongjun Zhao, Claudia C. Faria, Jose Pimentel, Sofia Nunes, Tarek Shalaby, Michael Grotzer, Ian F. Pollack, Ronald L. Hamilton, Xiao-Nan Li, Anne E. Bendel, Daniel W. Fults, Andrew W. Walter, Toshihiro Kumabe, Teiji Tominaga, V. Peter Collins, Yoon-Jae Cho, Caitlin Hoffman, David Lyden, Jeffrey H. Wisoff, James H. Garvin, Duncan S. Stearns, Luca Massimi, Ulrich Schueller, Jaroslav Sterba, Karel Zitterbart, Stephanie Puget, Olivier Ayrault, Sandra E. Dunn, Daniela P. C. Tirapelli, Carlos G. Carlotti, Helen Wheeler, Andrew R. Hallahan, Wendy Ingram, Tobey J. MacDonald, Jeffrey J. Olson, Erwin G. Van Meir, Ji-Yeoun Lee, Kyu-Chang Wang, Seung-Ki Kim, Byung-Kyu Cho, Torsten Pietsch, Gudrun Fleischhack, Stephan Tippelt, Young Shin Ra, Simon Bailey, Janet C. Lindsey, Steven C. Clifford, Charles G. Eberhart, Michael K. Cooper, Roger J. Packer, Maura Massimino, Maria Luisa Garre, Ute Bartels, Uri Tabori, Cynthia E. Hawkins, Peter Dirks, Eric Bouffet, James T. Rutka, Robert J. Wechsler-Reya, William A. Weiss, Lara S. Collier, Adam J. Dupuy, Andrey Korshunov, David T. W. Jones, Marcel Kool, Paul A. Northcott, Stefan M. Pfister, David A. Largaespada, Andrew J. Mungall, Richard A. Moore, Nada Jabado, Gary D. Bader, Steven J. M. Jones, David Malkin, Marco A. Marra, Michael D. Taylor
Review
Cell Biology
Xi Huang, Lily Yeh Jan
JOURNAL OF CELL BIOLOGY
(2014)
Article
Multidisciplinary Sciences
Veronique Frattini, Stefano M. Pagnotta, Tala, Jerry J. Fan, Marco V. Russo, Sang Bae Lee, Luciano Garofano, Jing Zhang, Peiguo Shi, Genevieve Lewis, Heloise Sanson, Vanessa Frederick, Angelica M. Castano, Luigi Cerulo, Delphine C. M. Rolland, Raghvendra Mall, Karima Mokhtari, Kojo S. J. Elenitoba-Johnson, Marc Sanson, Xi Huang, Michele Ceccarelli, Anna Lasorella, Antonio Iavarone
Article
Biochemistry & Molecular Biology
Rintaro Hashizume, Noemi Andor, Yuichiro Ihara, Robin Lerner, Haiyun Gan, Xiaoyue Chen, Dong Fang, Xi Huang, Maxwell W. Tom, Vy Ngo, David Solomon, Sabine Mueller, Pamela L. Paris, Zhiguo Zhang, Claudia Petritsch, Nalin Gupta, Todd A. Waldman, C. David James
Article
Neurosciences
Xin Chen, Siyi Wanggou, Ankur Bodalia, Min Zhu, Weifan Dong, Jerry J. Fan, Wen Chi Yin, Hyun-Kee Min, Malini Hu, Diana Draghici, Wenkun Dou, Feng Li, Fiona J. Coutinho, Heather Whetstone, Michelle M. Kushida, Peter B. Dirks, Yuanquan Song, Chi-Chung Hui, Yu Sun, Lu-Yang Wang, Xuejun Li, Xi Huang
Article
Cell Biology
Beverly J. Piggott, Christian J. Peters, Ye He, Xi Huang, Susan Younger, Lily Yeh Jan, Yuh Nung Jan
GENES & DEVELOPMENT
(2019)
Article
Multidisciplinary Sciences
Hiromichi Suzuki, Sachin A. Kumar, Shimin Shuai, Ander Diaz-Navarro, Ana Gutierrez-Fernandez, Pasqualino De Antonellis, Florence M. G. Cavalli, Kyle Juraschka, Hamza Farooq, Ichiyo Shibahara, Maria C. Vladoiu, Jiao Zhang, Namal Abeysundara, David Przelicki, Patryk Skowron, Nicole Gauer, Betty Luu, Craig Daniels, Xiaochong Wu, Antoine Forget, Ali Momin, Jun Wang, Weifan Dong, Seung-Ki Kim, Wieslawa A. Grajkowska, Anne Jouvet, Michelle Fevre-Montange, Maria Luisa Garre, Amulya A. Nageswara Rao, Caterina Giannini, Johan M. Kros, Pim J. French, Nada Jabado, Ho-Keung Ng, Wai Sang Poon, Charles G. Eberhart, Ian F. Pollack, James M. Olson, William A. Weiss, Toshihiro Kumabe, Enrique Lopez-Aguilar, Boleslaw Lach, Maura Massimino, Erwin G. Van Meir, Joshua B. Rubin, Rajeev Vibhakar, Lola B. Chambless, Noriyuki Kijima, Almos Klekner, Laszlo Bognar, Jennifer A. Chan, Claudia C. Faria, Jiannis Ragoussis, Stefan M. Pfister, Anna Goldenberg, Robert J. Wechsler-Reya, Swneke D. Bailey, Livia Garzia, A. Sorana Morrissy, Marco A. Marra, Xi Huang, David Malkin, Olivier Ayrault, Vijay Ramaswamy, Xose S. Puente, John A. Calarco, Lincoln Stein, Michael D. Taylor
Article
Immunology
Michelle A. Francisco, Siyi Wanggou, Jerry J. Fan, Weifan Dong, Xin Chen, Ali Momin, Namal Abeysundara, Hyun-Kee Min, Jade Chan, Rochelle McAdam, Marian Sia, Ronwell J. Pusong, Shixuan Liu, Nish Patel, Vijay Ramaswamy, Noriyuki Kijima, Lu-Yang Wang, Yuanquan Song, Ran Kafri, Michael D. Taylor, Xuejun Li, Xi Huang
JOURNAL OF EXPERIMENTAL MEDICINE
(2020)
Review
Oncology
Uswa Shahzad, Michael S. Taccone, Sachin A. Kumar, Hidehiro Okura, Stacey Krumholtz, Joji Ishida, Coco Mine, Kyle Gouveia, Julia Edgar, Christian Smith, Madeline Hayes, Xi Huang, W. Brent Derry, Michael D. Taylor, James T. Rutka
Summary: This review discusses the benefits of using non-murine species in cancer research, such as fruit flies, nematodes, and zebrafish, to study molecular processes related to cell development and cancer. These organisms have been found to share high genetic homology with humans and offer advantages such as shorter lifespans and suitability for high-throughput drug screening. The proposal suggests that utilizing these powerful screening tools and strategies can expedite discoveries in the field of cancer research and neuro-oncology.
Review
Pharmacology & Pharmacy
Ali Momin, Shahrzad Bahrampour, Hyun-Kee Min, Xin Chen, Xian Wang, Yu Sun, Xi Huang
Summary: Cells generate, transmit, and sense mechanical forces, shaping the mechanical properties of cells and tissues; in the brain, disrupted mechanical properties are observed in cancer states. Mechanosensitive ion channels govern ion flux and cellular signaling in response to mechanical cues, offering insights for therapeutic opportunities in brain tumors at the interface of cancer, neuroscience, and mechanobiology.
TRENDS IN PHARMACOLOGICAL SCIENCES
(2021)
Article
Cell Biology
Michael Pryszlak, Mallory Wiggans, Xin Chen, Julia E. Jaramillo, Sarah E. Burns, Laura M. Richards, Trevor J. Pugh, David R. Kaplan, Xi Huang, Peter B. Dirks, Bret J. Pearson
Summary: By using a comparative genomics approach, researchers have identified and demonstrated the role of DEAD-box helicase DDX56 in regulating stemness in different cell systems. DDX56 affects ribosomal RNA expression and nucleolar integrity in planarians, mouse neural stem cells, human GSCs, and a fruit fly model, ultimately leading to stem cell death.
Article
Cell & Tissue Engineering
Nicole I. Park, Paul Guilhamon, Kinjal Desai, Rochelle F. McAdam, Ellen Langille, Madlen O'Connor, Xiaoyang Lan, Heather Whetstone, Fiona J. Coutinho, Robert J. Vanner, Erick Ling, Panagiotis Prinos, Lilian Lee, Hayden Selvadurai, Gurnit Atwal, Michelle Kushida, Ian D. Clarke, Veronique Voisin, Michael D. Cusimano, Mark Bernstein, Sunit Das, Gary Bader, Cheryl H. Arrowsmith, Stephane Angers, Xi Huang, Mathieu Lupien, Peter B. Dirks
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)