Article
Engineering, Biomedical
Soja Saghar Soman, Mano Govindraj, Noura Al Hashimi, Jiarui Zhou, Sanjairaj Vijayavenkataraman
Summary: Bioprinting of neural tissue is a significant challenge in the field, with a focus on promoting growth and differentiation. A novel bioink formulation enables seamless bioprinting and subsequent cell recovery and proliferation.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2022)
Article
Biochemistry & Molecular Biology
Yilin Han, Marianne King, Evgenii Tikhomirov, Povilas Barasa, Cleide Dos Santos Souza, Jonas Lindh, Daiva Baltriukiene, Laura Ferraiuolo, Mimoun Azzouz, Maurizio R. Gullo, Elena N. Kozlova
Summary: The study aims to determine the survival and differentiation of boundary cap neural crest stem cells (BC) in gelatin-based 3D bioprinted bioink scaffolds for the fabrication of spinal cord organoids on a chip. The gelatin-based and enzymatically cross-linked hydrogel was found to be a suitable bioink for building a multicellular, bioprinted spinal cord organoid, but further measures are needed to achieve uniform neural differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Guan-Yu Zhang, Zhu-Man Lv, Hao-Xin Ma, Yu Chen, Yuan Yuan, Ping-Xin Sun, Yu-Qi Feng, Ya-Wen Li, Wen-Jie Lu, Yu-Dong Yang, Cheng Yang, Xin-Lu Yu, Chao Wang, Shu-Long Liang, Ming-Liang Zhang, Hui-Liang Li, Wen-Lin Li
Summary: This study has developed an efficient method to induce pMN progenitors from human embryonic stem cells. These pMN progenitors can be stably maintained for multiple passages and retain their ability to rapidly produce spinal motor neurons and oligodendrocytes. These self-renewing pMN progenitors may have important clinical implications for the treatment of spinal cord injury and demyelinating disorders.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Engineering, Biomedical
Lothar Koch, Andrea Deiwick, Jordi Soriano, Boris Chichkov
Summary: Generation of human neuronal networks by 3D bioprinting using hiPSC-derived NSCs and neurons was studied. The inclusion of astrocytes in the printing process was found to affect NSCs but not neurons. Differentiation stage and droplet size were also found to impact cell viability and neuronal dendrite formation.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Nanoscience & Nanotechnology
Xingliang Dai, Yuxuan Shao, Xuefeng Tian, Xiaoyan Cao, Lei Ye, Peng Gao, Hongwei Cheng, Xianwen Wang
Summary: Cell fusion between GSCs and MSCs in 3D-bioprinted models promotes the progression of glioma by enhancing the malignant properties of the fused cells.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Milena Restan Perez, Ruchi Sharma, Nadia Zeina Masri, Stephanie Michelle Willerth
Summary: Current treatments for neurodegenerative diseases aim to alleviate symptoms but do not cure or prevent degeneration; Bioprinting engineered neural tissues from patient-derived MSCs shows potential for personalized disease models and drug screening.
Article
Engineering, Environmental
Chen Gao, Yuxuan Li, Xiaoyun Liu, Jie Huang, Zhijun Zhang
Summary: In this study, conductive hydrogels were developed using gelatin methacrylate (GelMA), hyaluronic acid methacrylate (HAMA) and poly(3,4-ethylenedioxythiophene): sulfonated lignin (PEDOT:LS). These hydrogels, incorporated into a biomimetic scaffold fabricated by 3D bioprinting, showed improved electrical conductivity and mechanical properties similar to native spinal cord tissues. In vitro and in vivo experiments demonstrated that the conductive biomimetic scaffold promoted neuronal differentiation, regeneration, and recovery of motor function in a rat spinal cord injury model. This study represents a promising approach for stem cell-based treatment of spinal cord injuries.
CHEMICAL ENGINEERING JOURNAL
(2023)
Review
Cell Biology
Atefeh Zarepour, Sara Hooshmand, Aylin Gokmen, Ali Zarrabi, Ebrahim Mostafavi
Summary: The combination of stem cells and 3D bioprinting shows promise for treating spinal cord injuries by replacing dead cells with multipotent stem cells and protecting them with bioprinted scaffolds to promote differentiation and functional integration at the injury site.
Article
Biology
Yunqin Wu, Xiaohua Peng, Song Ang, Yue Gao, Yue Chi, Jinling Wang, Chengcheng Tang, Xiaoqing Zhou, Yanxian Feng, Kun Zhang, Qingjian Zou, Min Chen
Summary: In this study, genetically modified neural stem cells (NSCs) were developed to enhance the survival and formation of motor neurons. The modified NSCs exhibited improved resistance to stress and toxicity, and successfully differentiated into motor neurons.
Article
Cell Biology
T. Hartung, M. Rhein, N. Kalmbach, N. Thau-Habermann, M. Naujock, L. Mueschen, H. Frieling, J. Sterneckert, A. Hermann, F. Wegner, S. Petri
Summary: ALS is a rapidly progressive disease affecting motor neurons, and epigenetic modifications of gene expression have been identified as potential mechanisms. This study found significant differences in FUS gene expression and promotor methylation in mutant FUS motor neurons compared to healthy controls, suggesting that targeting disturbed methylation could potentially be a novel therapeutic strategy for ALS.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Amanda C. Juraski, Sonali Sharma, Sydney Sparanese, Victor A. da Silva, Julie Wong, Zachary Laksman, Ryan Flannigan, Leili Rohani, Stephanie M. Willerth
Summary: This article introduces the application of 3D printing technology in drug discovery, focusing on automating tissue production for high-throughput drug screening. The authors also discuss the considerations when using 3D bioprinting to generate cell laden constructs for drug screening, as well as the evaluation of potential drug candidates. The article highlights the use of bioprinting in generating cardiac, neural, and testis tissue models.
EXPERT OPINION ON DRUG DISCOVERY
(2023)
Article
Developmental Biology
Kayt Scott, Rebecca O'Rourke, Caitlin C. Winkler, Christina A. Kearns, Bruce Appel
Summary: Single-cell RNA sequencing of pMN cells in embryonic zebrafish trunk tissue revealed two distinct progenitor populations: one generating neurons and the other generating OPCs. A subpopulation called Pre-OPCs, marked by the expression of the homeobox transcription factor gene gsx2, was identified in the spinal cord prior to canonical OPC marker gene expression.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Tosho Kondo, Ihori Ebinuma, Hirotaka Tanaka, Yukitoshi Nishikawa, Takaki Komiya, Mitsuru Ishikawa, Hideyuki Okano
Summary: In this study, an effective high-throughput screening system was established using induced pluripotent stem cells (iPSCs) to generate motor neurons. The generated motor neurons exhibited characteristics similar to ALS neurons and showed abnormal protein accumulation and hyperexcitability. Treatment with rapamycin and retigabine normalized activity and improved neuronal survival. This study provides a valuable platform for discovering novel ALS therapeutics and personalized medicine approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Jessica Aijia Liu, Kin Wai Tam, Yong Long Chen, Xianglan Feng, Christy Wing Lam Chan, Amos Lok Hang Lo, Kenneth Lap-Kei Wu, Man-Ning Hui, Ming-Hoi Wu, Ken Kwok-Keung Chan, May Pui Lai Cheung, Chi Wai Cheung, Daisy Kwok-Yan Shum, Ying-Shing Chan, Martin Cheung
Summary: The study found that neural stem cells derived from human pluripotent stem cells have therapeutic potential for spinal cord injury. By reducing the expression of the SOX9 gene, the neurogenic capacity of the transplanted cells is enhanced, leading to improved integration and functional recovery.
Article
Engineering, Biomedical
Xiaoyun Liu, Mingming Hao, Zhongjin Chen, Ting Zhang, Jie Huang, Jianwu Dai, Zhijun Zhang
Summary: 3D bioprinting has been used to fabricate NSC-laden scaffolds for in vivo SCI repair, utilizing a novel bioink with fast gelation and high NSC viability. This method promotes axon regeneration and reduces glial scar deposition, leading to significant locomotor recovery in SCI model rats. It represents a versatile strategy for precise engineering of the central nervous system and other neural tissues for regenerative medicine applications.
Review
Biochemistry & Molecular Biology
Federica Rey, Letizia Messa, Erika Maghraby, Giovanna Casili, Sara Ottolenghi, Bianca Barzaghini, Manuela Teresa Raimondi, Cristina Cereda, Salvatore Cuzzocrea, Gianvincenzo Zuccotti, Emanuela Esposito, Irene Paterniti, Stephana Carelli
Summary: Oxygen (O-2) sensing plays a crucial role in the pathogenesis of neurodegenerative diseases (NDs) such as Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis. Common pathways related to O-2 imbalance include hypoxia, hyperoxia, reactive oxygen species production, metabolism of metals, protein misfolding, and neuroinflammation.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Letter
Medical Laboratory Technology
Simona Ferraro, Cristina Cereda, Gianvincenzo Zuccotti, Elia Mario Biganzoli
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Simona Ferraro, Ester Luconi, Valeria Calcaterra, Erika Cordaro, Alice Bianchi, Cristina Cereda, Gianvincenzo Zuccotti, Peter Kavsak, Mario Plebani, Elia Mario Biganzoli, Giuseppe Marano, Patrizia Boracchi
Summary: A common lower reference limit (LRL) can be utilized for TSH, FT4, and FT3 in patients aged <= 18 years, while age-specific upper reference limits (URLs) are recommended.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Clinical Neurology
Eleonora Bonaventura, Luisella Alberti, Simona Lucchi, Laura Cappelletti, Salvatore Fazzone, Elisa Cattaneo, Matteo Bellini, Giana Izzo, Cecilia Parazzini, Alessandra Bosetti, Elisabetta Di Profio, Giulia Fiore, Matilde Ferrario, Chiara Mameli, Arianna Sangiorgio, Silvia Masnada, Gian Vincenzo Zuccotti, Pierangelo Veggiotti, Luigina Spaccini, Maria Iascone, Elvira Verduci, Cristina Cereda, Davide Tonduti
Summary: X-linked adrenoleukodystrophy (X-ALD) is a common genetic disorder caused by variants in the ABCD1 gene. Early diagnosis and treatment can improve patient outcomes and increase survival rates.
FRONTIERS IN NEUROLOGY
(2023)
Article
Genetics & Heredity
Alessia Mauri, Laura Assunta Saielli, Enrico Alfei, Maria Iascone, Daniela Marchetti, Elisa Cattaneo, Anna Di Lauro, Laura Antonelli, Luisella Alberti, Eleonora Bonaventura, Pierangelo Veggiotti, Luigina Spaccini, Cristina Cereda
Summary: In this study, we report an infant with both Menkes disease and Aminoacylase-1 deficiency, characterized by neurological symptoms such as seizures, neurodevelopmental delay, and hypotonia. Genetic sequencing revealed mutations in the ATP7A and ACY1 genes, contributing to the development of these two rare conditions. This research expands the mutation spectrum of the ATP7A gene.
FRONTIERS IN GENETICS
(2023)
Article
Cell Biology
Clarissa Berardo, Valeria Calcaterra, Alessia Mauri, Stephana Carelli, Letizia Messa, Francesca Destro, Federica Rey, Erika Cordaro, Gloria Pelizzo, Gianvincenzo Zuccotti, Cristina Cereda
Summary: The prevalence of pediatric obesity is increasing worldwide. This study investigates the transcriptional differences in the subcutaneous adipose tissue of children with different weight status. The results show significant deregulation in both coding and non-coding transcripts in the severe obesity group compared to other weight groups. The deregulation is mainly related to lipid metabolism and bioenergetic processes.
Review
Biochemistry & Molecular Biology
Federica Rey, Clarissa Berardo, Erika Maghraby, Alessia Mauri, Letizia Messa, Letizia Esposito, Giovanna Casili, Sara Ottolenghi, Eleonora Bonaventura, Salvatore Cuzzocrea, Gianvincenzo Zuccotti, Davide Tonduti, Emanuela Esposito, Irene Paterniti, Cristina Cereda, Stephana Carelli
Summary: Oxygen imbalance can have detrimental effects on the brain, leading to various neurological disorders. The dysfunctions caused by oxygen imbalance in neurodegenerative and pediatric neurological disorders result from redox imbalance. This review focuses on the underlying dysfunction in redox and potential therapeutic strategies for these conditions.
Article
Engineering, Biomedical
Francesca Donnaloja, Manuela Teresa Raimondi, Letizia Messa, Bianca Barzaghini, Federica Carnevali, Emanuele Colombo, Davide Mazza, Chiara Martinelli, Lucia Boeri, Federica Rey, Cristina Cereda, Roberto Osellame, Giulio Cerullo, Stephana Carelli, Monica Soncini, Emanuela Jacchetti
Summary: Mechanical stimuli from the extracellular environment affect cell morphology and functionality. The use of a custom-made 3D microscaffold, called the Nichoid, for cultivating mesenchymal stem cells (MSCs) has been shown to enhance the expression of stemness markers. This device appears to regulate gene activity by altering intracellular force transmission. The findings suggest that the Nichoid can influence MSC behavior and gene regulation through mechanical manipulation.
APL BIOENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Paola De Filippi, Edoardo Errichiello, Antonio Toscano, Tiziana Mongini, Maurizio Moggio, Sabrina Ravaglia, Massimiliano Filosto, Serenella Servidei, Olimpia Musumeci, Fabio Giannini, Alberto Piperno, Gabriele Siciliano, Giulia Ricci, Antonio Di Muzio, Miriam Rigoldi, Paola Tonin, Michele Giovanni Croce, Elena Pegoraro, Luisa Politano, Lorenzo Maggi, Roberta Telese, Alberto Lerario, Cristina Sancricca, Liliana Vercelli, Claudio Semplicini, Barbara Pasanisi, Bruno Bembi, Andrea Dardis, Ilaria Palmieri, Cristina Cereda, Enza Maria Valente, Cesare Danesino
Summary: Pompe disease is a genetic disorder caused by mutations in the GAA gene that result in glycogen storage in lysosomes, particularly in muscle tissue. The relationship between genotype and phenotype has been extensively discussed, suggesting that other genetic variants may influence the clinical presentation. In this study, we identified potentially relevant variants in genes related to glycogen synthesis and catabolism in late-onset Pompe disease patients.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Review
Chemistry, Medicinal
Cecilia Pandini, Federica Rey, Cristina Cereda, Stephana Carelli, Paolo Gandellini
Summary: Long non-coding RNAs (lncRNAs) play crucial roles in pediatric neurological diseases and have implications for neural development and disorders. Further research is needed to fully understand the involvement of lncRNAs in pediatric neurological diseases and brain tumors, offering potential therapeutic targets and biomarkers.
Meeting Abstract
Biochemistry & Molecular Biology
Alessia Mauri, Alessandra Duse, Sara Olivotto, Stefania Bova, Pierangelo Veggiotti, Cristina Cereda
EUROPEAN JOURNAL OF HUMAN GENETICS
(2023)
Meeting Abstract
Cell & Tissue Engineering
Bianca Barzaghini, Letizia Messa, Federica Rey, Maria Antonietta Avanzini, Emanuela Jacchetti, Erika Maghrabys, Clarissa Berardo, Gianvincenzo Zuccotti, Cristina Cereda, Valeria Calcaterra, Gloria Pelizzo, Manuela Teresa Raimondi, Stephana Carelli
TISSUE ENGINEERING PART A
(2023)
Meeting Abstract
Cell & Tissue Engineering
Bianca Barzaghini, Letizia Messa, Federica Rey, Francesca Fanizza, Maria Antonella Sforazzini, Letizia Esposito, Gianvincenzo Zuccotti, Cristina Cereda, Roberto Osellame, Giulio Cerullo, Emanuela Jacchetti, Stephana Carelli, Manuela Teresa Raimondi
TISSUE ENGINEERING PART A
(2023)