Article
Cell Biology
Anna Frappaolo, Roberto Piergentili, Maria Grazia Giansanti
Summary: Drosophila dividing spermatocytes provide a suitable cell system to study the reorganization of microtubule and actin cytoskeleton systems during cell division. The unique characteristics of Drosophila male meiotic cells make them amenable to investigate the molecular pathways regulating spindle microtubules and cytokinesis, with important implications for cancer and other diseases.
Article
Cell Biology
Wen Gao, Chen Zhang, Bichun Li, Jeong Su Oh
Summary: Azoxystrobin (AZO), a widely used fungicide in agriculture, has been found to impair mouse oocyte maturation by disturbing spindle formation, chromosome alignment, mitochondrial distribution, and increasing oxidative stress. The toxicity of AZO to oocytes can be relieved by melatonin supplementation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Ying Chen, Chen Pan, Yajuan Lu, Yilong Miao, Bo Xiong
Summary: In this study, we found that HDAC8 plays a key role in regulating spindle assembly during porcine oocyte meiotic maturation. Depletion or inhibition of HDAC8 led to oocyte meiotic failure, including reduced polar body extrusion rate and aberrant spindle morphologies. The results suggest that HDAC8 is essential for proper chromosome alignment and spindle dynamics in female germ cell development.
CELL PROLIFERATION
(2021)
Article
Engineering, Environmental
Jiyeon Leem, Seul Kim, Jae-Sung Kim, Jeong Su Oh
Summary: In this study, the effects of exposure to 9,10-phenanthrenequinone (9,10-PQ) were investigated using mouse oocytes as a model system. It was found that 9,10-PQ compromised meiotic maturation by impairing the assembly of the microtubule organizing center and subsequent spindle formation. Additionally, 9,10-PQ exposure inhibited cell cycle progression by disrupting the accumulation of cyclin B1 and the activation of Cdk1. Importantly, the meiotic defects induced by 9,10-PQ were not rescued by decreasing reactive oxygen species (ROS) levels, indicating that 9,10-PQ has ROS-independent activity in regulating cell cycle progression and spindle assembly.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Cell Biology
Yu-Jin Jo, Jeongwoo Kwon, Zhe-Long Jin, Suk Namgoong, Taeho Kwon, Seung-Bin Yoon, Dong-Ho Lee, Ji-Su Kim, Nam-Hyung Kim
Summary: WHAMM plays a crucial role in mammalian oocyte maturation by regulating spindle formation and chromosome alignment, ultimately impacting the symmetry of cell division.
Article
Biochemistry & Molecular Biology
Guangyi Cao
Summary: The correct assembly and regulation of the spindle apparatus are crucial for the precise separation of chromosomes in mouse oocytes. NUSAP1 is a spindle-associated protein that plays an important role in oocyte meiosis maturation. Its depletion leads to chromosome misalignment, aneuploidy, and abnormal spindle assembly.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Cell Biology
Hao Qin, Yi Qu, Yi-Feng Yuan, Yang-Yang Li, Jie Qiao
Summary: This study revealed that RBM14 is down-regulated in oocytes from old mice and shows overlapped localization patterns with alpha-tubulin in different stages of meiosis. Knockdown of RBM14 may result in symmetric division of oocytes, spindle defects, and chromosome abnormalities. Additionally, RBM14 interacts with endogenous alpha-tubulin and modulates oocyte meiotic maturation by affecting alpha-tubulin acetylation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Song-Hee Lee, Ming-Hong Sun, Dongjie Zhou, Wen-Jie Jiang, Xiao-Han Li, Geun Heo, Xiang-Shun Cui
Summary: Exposure to high temperature has been found to negatively affect the development of porcine oocytes. It induces mitochondrial dysfunction, ER stress, abnormal Golgi apparatus distribution, and lysosomal damage, leading to the failure of meiotic maturation in the oocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Reproductive Biology
Yue Wang, Chun-Hua Xing, Shun Chen, Shao-Chen Sun
Summary: The study showed that ZEN exposure disrupted organelle function in porcine oocyte meiotic maturation, affecting mitochondria, endoplasmic reticulum, Golgi apparatus, and lysosome.
Article
Cell Biology
Yumi Hoshino, Takafumi Uchida
Summary: Pin1, an enzyme that regulates the function of phosphorylated proteins, plays a crucial role in meiotic maturation. By altering the structure of proline peptide bond in proteins, Pin1 controls the progression of meiosis and affects the extrusion of polar bodies. The functional regulation of Pin1 is essential for oocyte production and holds potential applications in reproductive medicine and animal breeding.
Article
Reproductive Biology
Naru Zhou, Qiuchen Liu, Xin Qi, Xiangdong Zhang, Zhenyuan Ru, Yangyang Ma, Tong Yu, Mianqun Zhang, Yunsheng Li, Yunhai Zhang, Zubing Cao
Summary: Paraquat exposure has toxic effects on porcine oocyte maturation, inhibiting cumulus cell expansion, reducing the rate of first polar body extrusion, and preventing oocytes from developing to later stages. These effects are likely mediated through oxidative stress, as indicated by changes in spindle structure, chromosome alignment, F-actin distribution, mitochondrial activity, and levels of reactive oxygen species, rH2AX, and the autophagy marker LC3. Additionally, gene expression associated with cumulus cell expansion is affected by PQ exposure, but not apoptosis-related genes.
Article
Genetics & Heredity
Marilia Korbes Rockenbach, Lucas Rosa Fraga, Thayne Woycinck Kowalski, Maria Teresa Vieira Sanseverino
Summary: Gametes are specialized cells that give rise to a totipotent zygote during fertilization. This study explores the differential gene expression of meiosis-related genes in male and female gonads and gametes in normal and pathological conditions. The results provide insights into potential genes relevant to human fertility disorders.
FRONTIERS IN GENETICS
(2023)
Article
Environmental Sciences
Kang-Na Wei, Xin-Jie Wang, Zhao-Cheng Zeng, Ruo-Ting Gu, Shu-Zi Deng, Jiang Jiang, Chang-Long Xu, Wei Li, Hai-Long Wang
Summary: The study revealed the toxic effects of PFOS on mouse oocyte maturation, including reduced polar body extrusion rate and symmetrical cell division. PFOS caused abnormal cytoskeleton in oocytes, inhibited meiotic progression, and disrupted oocyte quality and histone modifications.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Review
Biochemistry & Molecular Biology
Pawel Kordowitzki, Gabriela Sokolowska, Marta Wasielak-Politowska, Agnieszka Skowronska, Mariusz T. Skowronski
Summary: The oocyte plays a crucial role in embryo development, with cell-to-cell and cell-to-matrix communications being key for oocyte's developmental competence. Pannexins and Connexins are families of channels that are important for various physiological and pathological processes related to oocyte development and fertility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Qi Jiang, Xin Qi, Chi Ding, Xingyu Liu, Yuanyuan Lei, Siying Li, Zubing Cao
Summary: Environmental pollutant dimethoate exposure impairs porcine oocyte maturation, but melatonin supplementation can restore meiotic maturation by suppressing the generation of reactive oxygen species, autophagy, and DNA damage accumulation. These findings suggest that melatonin could be a promising agent in improving the quality of dimethoate-exposed oocytes from humans and animals.
Review
Biochemistry & Molecular Biology
Anna Bielak-Zmijewska, Wioleta Grabowska, Agata Ciolko, Agnieszka Bojko, Grazyna Mosieniak, Lukasz Bijoch, Ewa Sikora
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Multidisciplinary Sciences
Katarzyna Filimonow, Nestor Saiz, Aneta Suwinska, Tomasz Wyszomirski, Joanna B. Grabarek, Elisabetta Ferretti, Anna Piliszek, Berenika Plusa, Marek Maleszewski
Article
Geriatrics & Gerontology
Wioleta Grabowska, Grazyna Mosieniak, Natalia Achtabowska, Robert Czochara, Grzegorz Litwinienko, Agnieszka Bojko, Ewa Sikora, Anna Bielak-Zmijewska
Review
Nutrition & Dietetics
Agnieszka Gadecka, Anna Bielak-Zmijewska
Article
Geriatrics & Gerontology
Kinga Jaworska, Marek Konop, Tomasz Hutsch, Karol Perlejewski, Marek Radkowski, Marta Grochowska, Anna Bielak-Zmijewska, Grazyna Mosieniak, Ewa Sikora, Marcin Ufnal
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2020)
Article
Food Science & Technology
Kinga Jaworska, Dagmara Hering, Grazyna Mosieniak, Anna Bielak-Zmijewska, Marta Pilz, Michal Konwerski, Aleksandra Gasecka, Agnieszka Kaplon-Cieslicka, Krzysztof Filipiak, Ewa Sikora, Robert Holyst, Marcin Ufnal
Article
Cell Biology
Ewa Sikora, Anna Bielak-Zmijewska, Grazyna Mosieniak
AGEING RESEARCH REVIEWS
(2019)
Article
Multidisciplinary Sciences
Aleksandra Bilska, Monika Kusio-Kobialka, Pawel S. Krawczyk, Olga Gewartowska, Bartosz Tarkowski, Kamil Kobylecki, Dominika Nowis, Jakub Golab, Jakub Gruchota, Ewa Borsuk, Andrzej Dziembowski, Seweryn Mroczek
NATURE COMMUNICATIONS
(2020)
Article
Reproductive Biology
Katarzyna Czajkowska, Agnieszka Walewska, Takao Ishikawa, Katarzyna Szczepanska, Anna Ajduk
BIOLOGY OF REPRODUCTION
(2020)
Article
Cell Biology
Wioleta Grabowska, Natalia Achtabowska, Agata Klejman, Krzysztof Skowronek, Malgorzata Calka, Anna Bielak-Zmijewska
MECHANISMS OF AGEING AND DEVELOPMENT
(2020)
Review
Geriatrics & Gerontology
Ewa Sikora, Anna Bielak-Zmijewska, Magdalena Dudkowska, Adam Krzystyniak, Grazyna Mosieniak, Malgorzata Wesierska, Jakub Wlodarczyk
Summary: Aging of the brain can result in memory and cognitive decline, often coinciding with changes in the structural plasticity of dendritic spines. Decreased number and maturity of spines in aged individuals, along with alterations in synaptic transmission, may reflect impaired brain functions. The involvement of cellular senescence in brain aging is being increasingly explored, with evidence suggesting a potential role in the aging process similar to other organs.
FRONTIERS IN AGING NEUROSCIENCE
(2021)
Review
Cell Biology
Ewa Sikora, Anna Bielak-Zmijewska, Grazyna Mosieniak
Summary: Cellular senescence is a significant biological phenomenon that mainly participates in aging and aging-related diseases through secretion activity and cell cycle arrest. In this process, DNA damage, nuclear changes, and chromatin rearrangement play important roles.
AGEING RESEARCH REVIEWS
(2021)
Article
Multidisciplinary Sciences
Katarzyna Krawczyk, Ewa Kosyl, Karolina Czescik-Lysyszyn, Tomasz Wyszomirski, Marek Maleszewski
Summary: The study found that twins and quadruplets differ in their ability to generate blastocyst lineages, with twins more likely to form diverse cell lineages in blastocysts while quadruplets have difficulty forming normal blastocysts. Some quadruplets even lack inner cell masses in blastocysts, which may explain the challenge of creating monozygotic twins and quadruplets from 2- and 4-cell stage embryos.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Katarzyna Krawczyk, Katarzyna Wilczak, Katarzyna Szczepanska, Marek Maleszewski, Aneta Suwinska
Summary: The early mammalian embryo has the potential for self-organization and correct patterning despite experimental perturbation. The restricted potential of the inner cell mass (ICM) can be compensated for by uncommitted blastomeres from 8-cell embryos, leading to the formation of a normally patterned chimaeric blastocyst. The presence and orientation of the polarized primitive endoderm layer in the ICM component play a role in maintaining developmental competence.
Article
Cell Biology
Adam Krzystyniak, Malgorzata Wesierska, Gregory Petrazzo, Agnieszka Gadecka, Magdalena Dudkowska, Anna Bielak-Zmijewska, Grazyna Mosieniak, Izabela Figiel, Jakub Wlodarczyk, Ewa Sikora
Summary: By conducting experiments on aged rats, it was found that the use of senolytic agents D+Q can improve the learning and memory abilities of aged rats, reduce peripheral inflammation, and regulate the structure of hippocampal neurons and histone modifications. Moreover, the benefits of this treatment are long-lasting, even after the cessation of drug administration.
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)