Article
Genetics & Heredity
Yi-Fan Liu, Ming Zhang, Yan-Ju Shan, Li-Chuan Pang, Gai-Ge Ji, Xiao-Jun Ju, Yun-Jie Tu, Shi-Ying Shi, Hao Bai, Jian-Min Zou, Jing-Ting Shu
Summary: This study investigates the effects of miR-499-5p and SOX6 on muscle fiber type and their regulatory mechanism. miR-499-5p overexpression leads to significant changes in gene expression, including genes related to slow-twitch muscle. Functional enrichment analysis shows that differentially expressed genes are mostly associated with muscle development and fiber-related processes.
FRONTIERS IN GENETICS
(2022)
Article
Multidisciplinary Sciences
Mi-Young Song, Chang Yeob Han, Young Jae Moon, Ju Hyung Lee, Eun Ju Bae, Byung-Hyun Park
Summary: Exercise can counteract obesity and its complications by switching muscle fibers to an oxidative type. Researchers have found that Sirtuin 6 overexpression in mice can induce this switch and may be a target for exercise mimetics. By activating Sirt6, skeletal muscle can be transformed into slow-twitch oxidative type, improving exercise capacity and mitigating obesity-related metabolic diseases.
NATURE COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Sharmin Hasan, Shuichi Asakawa, Shugo Watabe, Shigeharu Kinoshita
Summary: This study demonstrates the regulation of myh14 gene expression in zebrafish, including the importance of promoter regions in paralogs myh14-1 and myh14-3, as well as the essential role of miR-499 in guiding slow muscle fiber development during embryogenesis.
MARINE BIOTECHNOLOGY
(2021)
Article
Medicine, General & Internal
Xinyuan Han, Shunda Wang, Zhijun Yong, Xueting Zhang, Xuanqi Wang, Penghua You
Summary: This study investigates the mechanism of miR-499-5p in ameliorating atrial fibrillation (AF) in rats by regulating Sry-related high-mobility-group box 6 (SOX6). The results show that miR-499-5p is downregulated and SOX6 is upregulated in AF rats. Overexpression of miR-499-5p can shorten AF duration, alleviate atrial fibrosis, and decrease fibrosis markers. miR-499-5p targets SOX6 to suppress atrial fibrosis and downregulate p21, thus mitigating AF in rats.
Article
Biochemistry & Molecular Biology
Zihao Zhang, Shudai Lin, Wen Luo, Tuanhui Ren, Xing Huang, Wangyu Li, Xiquan Zhang
Summary: Adult skeletal muscle is divided into fast and slow-type muscles, and satellite cells play a crucial role in their development. The expression of Sox6 and Nfix partially explain the differences in development between satellite cells derived from fast and slow muscles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cardiac & Cardiovascular Systems
My-Anh Nguyen, Huy-Dung Hoang, Adil Rasheed, Anne-Claire Duchez, Hailey Wyatt, Mary Lynn Cottee, Tyson E. Graber, Leah Susser, Sabrina Robichaud, Ibrahim Berber, Michele Geoffrion, Mireille Ouimet, Hilal Kazan, Lars Maegdefessel, Erin E. Mulvihill, Tommy Alain, Katey J. Rayner
Summary: miR-223 acts as a key regulator in macrophages, protecting against atherosclerosis by modulating cholesterol metabolism and inflammation pathways.
CIRCULATION RESEARCH
(2022)
Article
Cell Biology
Xiwei Zhou, Bao Zheng, Lujun Pang, Yanjun Che, Xin Qi
Summary: Our study found upregulation of MALAT1 in SAH mice and OxyHb-stimulated neurons. Knockdown of MALAT1 reduced neurocyte apoptosis and ROS production, possibly through the miR-499-5p/SOX6 axis.
JOURNAL OF MOLECULAR HISTOLOGY
(2022)
Article
Oncology
Chao Liu, Wenhui Gao, Yijun Shi, Lu Lv, Weifeng Tang
Summary: This study investigated the relationship between miRNA rs2910164, rs11614913, and rs3746444 polymorphisms and the susceptibility to esophageal squamous cell cancer (ESCC) in the Chinese Han nationality. The results suggest that the rs3746444 GG genotype is associated with an increased risk of ESCC, while rs11614913 and rs2910164 polymorphisms are not associated with ESCC.
Article
Critical Care Medicine
Li Hu, Jie Wang, Huijie Huang, Yanfang Yu, Jingjing Ding, Youjia Yu, Kai Li, Dong Wei, Qing Ye, Fangzhu Wang, Bin Shen, Jingyu Chen, David J. R. Fulton, Feng Chen
Summary: This study demonstrates the involvement of m(6)A RNA modification and m(6)A effector proteins in pulmonary hypertension (PH), with YTHDF1 promoting PASMC proliferation and PH by enhancing MAGED1 translation. The findings suggest a novel mechanism for pathological changes in PASMCs and PH mediated by m(6)A RNA modification.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Farhad Tabasi, Vahed Hasanpour, Shamim Sarhadi, Mahmoud Ali Kaykhaei, Pouria Pourzand, Mehrdad Heravi, Ahmad Alinaghi Langari, Gholamreza Bahari, Mohsen Taheri, Mohammad Hashemi, Saeid Ghavami
Summary: The miR-499 rs3746444 polymorphism is positively associated with Hashimoto thyroiditis (HT) risk, and may contribute to the regulation of immune system pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Geriatrics & Gerontology
Ayoung Kim, Ja Hyun Koo, Xing Jin, Wondong Kim, Shi-Young Park, Sunghyouk Park, Eugene P. Rhee, Cheol Soo Choi, Sang Geon Kim
Summary: The research findings demonstrate the crucial role of USP21 in skeletal muscle in regulating myofibre type switch, muscle mass control, mitochondrial function, and heat generation, suggesting the potential of USP21 and its downstream substrates network as targets for treating and preventing muscle dysfunction and associated metabolic diseases.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2021)
Article
Geriatrics & Gerontology
Xiaoyan Shao, Wang Gong, Qianjin Wang, Pu Wang, Tianshu Shi, Abdurahman Mahmut, Jianghui Qin, Yao Yao, Wenjin Yan, Dongyang Chen, Xiang Chen, Qing Jiang, Baosheng Guo
Summary: This study found that sEV miR-690 derived from atrophic muscle fibers may inhibit satellite cell differentiation by targeting myocyte enhancer factor 2 during aging. This research contributes to understanding the mechanisms of muscle aging and atrophy, and has important implications for the prevention and treatment of muscle degenerative diseases.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2022)
Article
Multidisciplinary Sciences
Takumi Sugioka, Masashi Tanimoto, Shin-ichi Higashijima
Summary: This study demonstrates that larval zebrafish can finely control their posture through a body bend reflex. The authors also identify the neural circuits and muscles involved in this reflex.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Gabryela Kuhnen, Tiago Guedes Russomanno, Marta Murgia, Nicolas J. Pillon, Martin Schoenfelder, Henning Wackerhage
Summary: Adult skeletal muscle fibres can be classified into different types based on the expression of specific genes. The proportions of these muscle fibre types can be altered through gene gain or loss-of-function. In particular, transcriptional regulators play a significant role in regulating the expression of muscle fibre-specific genes. After exercise, the expression of certain genes increases or decreases, suggesting their involvement in muscle fibre adaptation. DNA sequence variants of muscle fibre genes may contribute to the variation in muscle fibre type proportions in the human population.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Michelle N. LeMaster, Robyn D. Warner, Surinder S. Chauhan, Darryl N. D'Souza, Frank R. Dunshea
Summary: This meta-regression analysis aimed to investigate the relationship between fibre type cross-sectional area (CSA) and frequency (%) and meat quality traits, specifically tenderness. The results showed that only pH, WBSF, and drip loss were significantly associated with fibre type frequency and CSA (p < 0.05). When limited to pork analysis, it was found that frequency of type I fibres was associated with decreased drip loss, increased cook loss, decreased lightness (L*), and increased sensory tenderness, while frequency of type IIb fibres was associated with increased drip loss (p < 0.05). Additionally, CSA of type I and IIb fibres was correlated with color traits of lightness and redness (p < 0.05). Further research should focus on different breeds and muscles to enhance understanding of the effects of fibre type frequency and CSA on meat quality.
Article
Biochemistry & Molecular Biology
Ganesh V. Pusapati, Jennifer H. Kong, Bhaven B. Patel, Mina Gouti, Andreas Sagner, Ria Sircar, Giovanni Luchetti, Philip W. Ingham, James Briscoe, Rajat Rohatgi
Article
Multidisciplinary Sciences
Irene Ojeda Naharros, Flavia B. Cristian, Jingjing Zang, Matthias Gesemann, Philip W. Ingham, Stephan C. F. Neuhauss, Ruxandra Bachmann-Gagescu
SCIENTIFIC REPORTS
(2018)
Article
Biochemistry & Molecular Biology
Ganesh V. Pusapati, Jennifer H. Kong, Bhaven B. Patel, Mina Gouti, Andreas Sagner, Ria Sircar, Giovanni Luchetti, Philip W. Ingham, James Briscoe, Rajat Rohatgi
Editorial Material
Biology
Timothy E. Saunders, Philip W. Ingham
Editorial Material
Cell Biology
Philip W. Ingham, Bruno Reversade
CURRENT OPINION IN GENETICS & DEVELOPMENT
(2019)
Article
Biology
Tsui Han Loo, Xiaoqian Ye, Ruth Jinfen Chai, Mitsuteru Ito, Gisele Bonne, Anne C. Ferguson-Smith, Colin L. Stewart
Article
Cell Biology
Josie F. Gibson, Tomasz K. Prajsnar, Christopher J. Hill, Amy K. Tooke, Justyna J. Serba, Rebecca D. Tonge, Simon J. Foster, Andrew J. Grierson, Philip W. Ingham, Stephen A. Renshaw, Simon A. Johnston
Summary: This study reveals the fate and location of Staphylococcus aureus within neutrophils, showing that recruitment of Lc3 and Sqstm1 to phagocytosed bacteria depends on bacterial location. Additionally, it demonstrates the key role of Sqstm1 in controlling cytosolic bacteria.
Article
Developmental Biology
Ilham J. J. Alshami, Yosuke Ono, Ana Correia, Christian Hacker, Anke Lange, Steffen Scholpp, Masashi Kawasaki, Philip W. Ingham, Tetsuhiro Kudoh
DEVELOPMENTAL BIOLOGY
(2020)
Article
Cell Biology
Bernadett Bosze, Yosuke Ono, Benjamin Mattes, Claude Sinner, A. Victor Gourain, Thomas Thumberger, Sham Tlili, Joachim Wittbrodt, Timothy E. Saundersz, Uwe Strahle, Alexander Schug, Steffen Scholpp
HISTOCHEMISTRY AND CELL BIOLOGY
(2020)
Article
Engineering, Biomedical
Cyrus W. Beh, Dionis S. Yew, Ruth J. Chai, Sau Yin Chin, Yiqi Seow, Shawn S. Hoon
Summary: The study introduces a hydrogel 3D printer that can print complex structures quickly and continuously, and is capable of multi-material printing. This innovative approach helps address the nutrient supply issue in bioprinting.
Article
Multidisciplinary Sciences
Ruth Jinfen Chai, Hendrikje Werner, Peter Yiqing Li, Yin Loon Lee, Khaing Thet Nyein, Irina Solovei, Tuan Danh Anh Luu, Bhavya Sharma, Raju Navasankari, Martina Maric, Lois Yu En Sim, Ying Jie Loh, Edita Aliwarga, Jason Wen Long Cheong, Alexandre Chojnowski, Matias Ilmari Autio, Yu Haiyang, Kenneth Kian Boon Tan, Choong Tat Keng, Shi Ling Ng, Wei Leong Chew, Michael Ferenczi, Brian Burke, Roger Sik Yin Foo, Colin L. Stewart
Summary: Mutations in the LaminA gene are a common cause of monogenic dilated cardiomyopathy. In mice, cardiomyocyte-specific expression of the SUN1 miniprotein can extend lifespan to more than one year, showing potential as a therapeutic option for treating this disease.
NATURE COMMUNICATIONS
(2021)
Article
Developmental Biology
Hussein A. Saud, Paul A. O'Neill, Yosuke Ono, Bas Verbruggen, Ronny Van Aerle, Jaebum Kim, Jae-Seong Lee, Brian C. Ring, Tetsuhiro Kudoh
Summary: This study characterized two mutants in self-fertilizing mangrove killifish, with identified mutated genes for rapid identification. The findings showcase the potential for accelerating future small-scale forward-genetic screening and mutation identification.
Article
Pharmacology & Pharmacy
Matthew J. Winter, Yosuke Ono, Jonathan S. Ball, Anna Walentinsson, Erik Michaelsson, Anna Tochwin, Steffen Scholpp, Charles R. Tyler, Steve Rees, Malcolm J. Hetheridge, Mohammad Bohlooly-Y
Summary: The clinical heterogeneity of heart failure poses challenges to the understanding of its genetic mechanisms. Large-scale patient DNA sequencing studies have become crucial for identifying potential genetic factors. Zebrafish provides a valuable model for high-throughput in vivo gene functional assessment, aiding target prioritization and facilitating gene selection for further experiments in mice. In this study, human patient data was analyzed using whole-exome sequencing and bioinformatics, and gene mutation analysis in zebrafish was conducted to investigate the potential roles of three genes in heart failure.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biology
Daniel Routledge, Sally Rogers, Yosuke Ono, Lucy Brunt, Valerie Meniel, Giusy Tornillo, Hassan Ashktorab, Toby J. Phesse, Steffen Scholpp
Summary: The study demonstrates that gastric cancer cells utilize cytonemes to transport Wnt3 protein intercellularly to promote cell proliferation and survival. Additionally, the membrane-bound scaffolding protein Flotillin-2 is identified as a regulator of these cytonemes in gastric cancer.